pycom05g02670

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
3150999 .. 3152547
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGCCTCCGATTCGCACCTCAAACACAAAGTCCATAAATAACACAACACAAAGTTGGATCAAAATATTGCAACCTACAATCCAAAAAAAAAATGGACGAGAAAGACGACAACAAAACCGGGGAAATCGAGGAAACGGGCCCAAAATCCGACGGGTCTTCTCCGAACCCGAATGCCAAAACTGCGAGGACGAAGGTTCCAGAAGTGGAAGTCTGCCTGTATCGACGGGGGAAAGGCCCGATCGACACGTTCAAGTCGGGTCTGGGGGGTTGGGACCAGAACCAGTTGGAGGTCGGAGACATTCTCGACAAGTATGGATTCAAATCGCTTTACGCTTTCAATACCGAATCGGGTCGCGGGGTTCATCGATCCGGTTTAACCCTAGAAACGGCAGGTCTATGCTGCCATATAGAGATGGAGCCACGGTTCAAATCGACGGAGAGCCTAAGGATTCATTGATCCAACCGGTGACGAAAATCTTGATTGGAGTAGCTTTTATGACCATCCTGATAACCTTGGTTTTGAGGGATCCTCCGCAATGGATCCAGAAATTAAACTTTACTGGAGGTAACTTCCCTCCATGGATCATCGCTCTCGTTCGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.59

Weight (kDa)

11.38

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 381
AccII CGCG 1 cut(s) 355
AciI CCGC 2 cut(s) 355, 533
AclWI GGATC 8 cut(s) 65, 361, 451, 521, 534, 535, 548, 590
AfiI CCNNNNNNNGG 2 cut(s) 287, 386
AflIII ACRYGT 1 cut(s) 244
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 4 cut(s) 251, 320, 338, 428
AloI GAACNNNNNNTCC 2 cut(s) 408, 440
AluBI AGCT 1 cut(s) 491
AluI AGCT 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 8 cut(s) 65, 361, 451, 521, 534, 535, 548, 590
AoxI GGCC 2 cut(s) 137, 233
ApaI GGGCCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 400
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AsiGI ACCGGT 1 cut(s) 463
AspS9I GGNCC 4 cut(s) 137, 138, 234, 272
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 478
AvaII GGWCC 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 444
BaeGI GKGCMC 1 cut(s) 141
BamHI GGATCC 2 cut(s) 526, 540
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 387
BccI CCATC 2 cut(s) 407, 509
BceAI ACGGC 1 cut(s) 403
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 381
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
Bme1390I CCNGG 1 cut(s) 119
Bme18I GGWCC 1 cut(s) 272
BmgT120I GGNCC 4 cut(s) 137, 138, 234, 272
BmiI GGNNCC 6 cut(s) 139, 196, 273, 418, 528, 542
BmrFI CCNGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 148
BplI GAGNNNNNCTC 4 cut(s) 286, 318, 514, 546
BpmI CTGGAG 1 cut(s) 582
BpuMI CCSGG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 320
BsaJI CCNNGG 4 cut(s) 118, 420, 513, 578
BsaWI WCCGGW 2 cut(s) 369, 463
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 386
Bse118I RCCGGY 1 cut(s) 463
Bse1I ACTGG 2 cut(s) 281, 565
Bse21I CCTNAGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 542
Bse8I GATNNNNATC 1 cut(s) 320
BseCI ATCGAT 1 cut(s) 365
BseDI CCNNGG 4 cut(s) 118, 420, 513, 578
BseGI GGATG 1 cut(s) 501
BseJI GATNNNNATC 1 cut(s) 320
BseLI CCNNNNNNNGG 2 cut(s) 287, 386
BseMI GCAATG 1 cut(s) 542
BseNI ACTGG 2 cut(s) 281, 565
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 355
Bsh1285I CGRYCG 1 cut(s) 241
BshFI GGCC 2 cut(s) 139, 235
BshTI ACCGGT 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 365
BsiEI CGRYCG 1 cut(s) 241
BsiSI CCGG 3 cut(s) 118, 370, 464
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 2 cut(s) 287, 386
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 285
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 2 cut(s) 139, 235
Bsp120I GGGCCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 2 cut(s) 355, 533
BspANI GGCC 2 cut(s) 139, 235
BspDI ATCGAT 1 cut(s) 365
BspFNI CGCG 1 cut(s) 355
BspLI GGNNCC 6 cut(s) 139, 196, 273, 418, 528, 542
BspMI ACCTGC 1 cut(s) 381
BspPI GGATC 8 cut(s) 65, 361, 451, 521, 534, 535, 548, 590
BsrDI GCAATG 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 463
BsrI ACTGG 2 cut(s) 281, 565
BssAI RCCGGY 1 cut(s) 463
BssECI CCNNGG 4 cut(s) 118, 420, 513, 578
BssMI GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
BssT1I CCWWGG 2 cut(s) 513, 578
Bst4CI ACNGT 1 cut(s) 424
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 2 cut(s) 420, 578
BstF5I GGATG 1 cut(s) 501
BstFNI CGCG 1 cut(s) 355
BstKTI GATC 8 cut(s) 60, 241, 369, 459, 529, 543, 585, 602
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
BstMCI CGRYCG 1 cut(s) 241
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 355
BstV1I GCAGC 1 cut(s) 387
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 526, 540
BstYI RGATCY 2 cut(s) 526, 540
Bsu15I ATCGAT 1 cut(s) 365
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 2 cut(s) 139, 235
BsuTUI ATCGAT 1 cut(s) 365
BtgI CCRYGG 2 cut(s) 420, 578
BtgZI GCGATG 1 cut(s) 571
BtsCI GGATG 1 cut(s) 501
BveI ACCTGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 463
Cfr13I GGNCC 4 cut(s) 137, 138, 234, 272
ClaI ATCGAT 1 cut(s) 365
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 1 cut(s) 579
CviJI RGCY 5 cut(s) 139, 235, 419, 442, 491
CviKI_1 RGCY 5 cut(s) 139, 235, 419, 442, 491
DdeI CTNAG 1 cut(s) 444
DpnI GATC 8 cut(s) 59, 240, 368, 458, 528, 542, 584, 601
DpnII GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
Eco130I CCWWGG 2 cut(s) 513, 578
Eco24I GRGCYC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 272
Eco81I CCTNAGG 1 cut(s) 444
EcoT14I CCWWGG 2 cut(s) 513, 578
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 513, 578
FaeI CATG 1 cut(s) 582
FaiI YATR 8 cut(s) 35, 313, 398, 406, 408, 497, 580, 604
FaqI GGGAC 1 cut(s) 285
FatI CATG 1 cut(s) 578
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 1 cut(s) 488
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 1 cut(s) 381
GluI GCNGC 1 cut(s) 401
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 2 cut(s) 139, 235
HapII CCGG 3 cut(s) 118, 370, 464
Hin1II CATG 1 cut(s) 582
HinfI GANTC 4 cut(s) 10, 316, 345, 449
HpaII CCGG 3 cut(s) 118, 370, 464
HphI GGTGA 1 cut(s) 478
Hpy188I TCNGA 4 cut(s) 9, 149, 163, 294
Hpy188III TCNNGA 5 cut(s) 198, 303, 478, 505, 544
Hpy99I CGWCG 3 cut(s) 153, 226, 437
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 1 cut(s) 70
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 1 cut(s) 582
Kzo9I GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 1 cut(s) 387
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 466, 566
MalI GATC 8 cut(s) 59, 240, 368, 458, 528, 542, 584, 601
MboI GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
MboII GAAGA 1 cut(s) 148
MflI RGATCY 2 cut(s) 526, 540
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 1 cut(s) 548
MmeI TCCRAC 5 cut(s) 35, 172, 265, 272, 484
MnlI CCTC 9 cut(s) 15, 28, 122, 178, 281, 516, 540, 557, 585
MseI TTAA 2 cut(s) 375, 551
MspI CCGG 3 cut(s) 118, 370, 464
MspR9I CCNGG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 176
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 180
NciI CCSGG 1 cut(s) 119
NcoI CCATGG 1 cut(s) 578
NdeII GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
NlaIII CATG 1 cut(s) 582
NlaIV GGNNCC 6 cut(s) 139, 196, 273, 418, 528, 542
NmuCI GTSAC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 103
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 4 cut(s) 10, 316, 345, 449
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 1 cut(s) 402
Ple19I CGATCG 1 cut(s) 241
PspN4I GGNNCC 6 cut(s) 139, 196, 273, 418, 528, 542
PspOMI GGGCCC 1 cut(s) 137
PspPI GGNCC 4 cut(s) 137, 138, 234, 272
PsuI RGATCY 2 cut(s) 526, 540
PvuI CGATCG 1 cut(s) 241
SaqAI TTAA 2 cut(s) 375, 551
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 8 cut(s) 57, 238, 366, 456, 526, 540, 582, 599
Sau96I GGNCC 4 cut(s) 137, 138, 234, 272
ScrFI CCNGG 1 cut(s) 119
SduI GDGCHC 1 cut(s) 141
SetI ASST 9 cut(s) 20, 76, 196, 249, 292, 395, 493, 515, 568
SinI GGWCC 1 cut(s) 272
Sse9I AATT 1 cut(s) 548
SsiI CCGC 2 cut(s) 355, 533
SspI AATATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 117
StyI CCWWGG 2 cut(s) 513, 578
TaaI ACNGT 1 cut(s) 424
TaiI ACGT 1 cut(s) 249
TaqI TCGA 7 cut(s) 127, 221, 241, 304, 365, 432, 598
TasI AATT 1 cut(s) 548
TfiI GAWTC 4 cut(s) 10, 316, 345, 449
Tru1I TTAA 2 cut(s) 375, 551
Tru9I TTAA 2 cut(s) 375, 551
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 2 cut(s) 351, 441
TspGWI ACGGA 1 cut(s) 450
VpaK11BI GGWCC 1 cut(s) 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.