pycom05g02740

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
3237644 .. 3238177
534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGGGGAACCCACCCCTTATCCCCTCACCCGTTCTTTCCTCTCCTGTCTTCCCCAAACCCAAATCCCACCCACCCTGCTCCCTTCTTCTTTGTGCTGATCATTGCCCGAGTCCCTCATCCCATAGTCCTCTCTTATCATCTTCGCTCCGCCAGACACTGCCTTCACCAAATCGGGTCAACCGGCCCTCTCTCTACCCCAGTACCACTTCTGCCCTCTTCGATCTCGAATTTACTCTGGCCACCCCCTCGCGAATTTACTCTGGCCACCCTCTTGTTGCCGAATCCGACGCCATACCTTACTTGTCTGAAACCTTCTTCAACTCCTCCCCTTCCCTCCAAGACGACGCCGAGGACACCACGGCGGTGATCGTGCATGTGGCCGAGTGCAGATTTGCTGGATCTCTTGACTGGGTCGAGCTTGAGGTGCAAGGAAAACGTGATGGGTGCACTGCTCAATTTGTCCATGTGCGGCAGAGAGAGGGCGTTGAGGGAGGTGAGGGAGGAGGGGCTAGGAGTGGCGGATGGGATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm

Protein Analysis

178

Amino Acids

18.93

Weight (kDa)

5.24

Isoelectric Point (pI)

66.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 250
AciI CCGC 4 cut(s) 148, 362, 469, 519
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 3 cut(s) 237, 262, 378
AcsI RAATTY 2 cut(s) 227, 252
AcyI GRCGYC 2 cut(s) 288, 345
AfaI GTAC 1 cut(s) 202
AgsI TTSAA 1 cut(s) 319
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 1 cut(s) 418
AluI AGCT 1 cut(s) 418
Alw21I GWGCWC 1 cut(s) 449
Alw44I GTGCAC 1 cut(s) 445
AlwI GGATC 1 cut(s) 406
AlwNI CAGNNNCTG 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 4 cut(s) 182, 237, 262, 378
ApaLI GTGCAC 1 cut(s) 445
ApoI RAATTY 2 cut(s) 227, 252
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 4 cut(s) 18, 156, 376, 506
AvaI CYCGRG 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 449
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BalI TGGCCA 2 cut(s) 239, 264
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 449
BccI CCATC 2 cut(s) 434, 516
BceAI ACGGC 1 cut(s) 375
BclI TGATCA 1 cut(s) 97
BfaI CTAG 1 cut(s) 510
BisI GCNGC 1 cut(s) 470
BlsI GCNGC 1 cut(s) 471
BmeT110I CYCGRG 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 8
BmrI ACTGGG 2 cut(s) 192, 418
BmuI ACTGGG 2 cut(s) 192, 418
BpiI GAAGAC 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 440
BsaHI GRCGYC 2 cut(s) 288, 345
BsaJI CCNNGG 2 cut(s) 348, 357
Bse118I RCCGGY 1 cut(s) 180
Bse1I ACTGG 2 cut(s) 198, 413
Bse3DI GCAATG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 348, 357
BseGI GGATG 2 cut(s) 116, 527
BseMI GCAATG 1 cut(s) 100
BseNI ACTGG 2 cut(s) 198, 413
BseRI GAGGAG 2 cut(s) 313, 516
BseSI GKGCMC 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 406
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 4 cut(s) 184, 239, 264, 380
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 1 cut(s) 181
BslFI GGGAC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 4 cut(s) 184, 239, 264, 380
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 4 cut(s) 97, 220, 366, 398
Bsp68I TCGCGA 1 cut(s) 250
BspACI CCGC 4 cut(s) 148, 362, 469, 519
BspANI GGCC 4 cut(s) 184, 239, 264, 380
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 8
BspPI GGATC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 180
BsrI ACTGG 2 cut(s) 198, 413
BssAI RCCGGY 1 cut(s) 180
BssECI CCNNGG 2 cut(s) 348, 357
BssMI GATC 4 cut(s) 97, 220, 366, 398
BssNI GRCGYC 2 cut(s) 288, 345
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 2 cut(s) 288, 345
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 2 cut(s) 116, 527
BstFNI CGCG 1 cut(s) 250
BstKTI GATC 4 cut(s) 100, 223, 369, 401
BstMBI GATC 4 cut(s) 97, 220, 366, 398
BstMWI GCNNNNNNNGC 1 cut(s) 424
BstNSI RCATGY 1 cut(s) 377
BstSLI GKGCMC 1 cut(s) 449
BstUI CGCG 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 4 cut(s) 184, 239, 264, 380
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 2 cut(s) 116, 527
BtsI GCAGTG 2 cut(s) 155, 447
BtsIMutI CAGTG 2 cut(s) 155, 447
BtuMI TCGCGA 1 cut(s) 250
CaiI CAGNNNCTG 1 cut(s) 157
Cfr10I RCCGGY 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 2 cut(s) 296, 353
Csp6I GTAC 1 cut(s) 201
CviAII CATG 2 cut(s) 374, 464
CviJI RGCY 6 cut(s) 184, 239, 264, 380, 418, 509
CviKI_1 RGCY 6 cut(s) 184, 239, 264, 380, 418, 509
CviQI GTAC 1 cut(s) 201
DpnI GATC 4 cut(s) 99, 222, 368, 400
DpnII GATC 4 cut(s) 97, 220, 366, 398
EaeI YGGCCR 3 cut(s) 237, 262, 378
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
EciI GGCGGA 2 cut(s) 137, 534
Eco88I CYCGRG 1 cut(s) 106
FaeI CATG 2 cut(s) 377, 467
FaiI YATR 4 cut(s) 123, 293, 375, 465
FaqI GGGAC 1 cut(s) 96
FatI CATG 2 cut(s) 373, 463
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 470
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 1 cut(s) 470
FspBI CTAG 1 cut(s) 510
GluI GCNGC 1 cut(s) 470
HaeIII GGCC 4 cut(s) 184, 239, 264, 380
HapII CCGG 1 cut(s) 181
HgaI GACGC 2 cut(s) 296, 353
Hin1I GRCGYC 2 cut(s) 288, 345
Hin1II CATG 2 cut(s) 377, 467
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 109, 281
HpaII CCGG 1 cut(s) 181
HphI GGTGA 4 cut(s) 18, 156, 376, 506
Hpy166II GTNNAC 2 cut(s) 178, 447
Hpy188I TCNGA 2 cut(s) 286, 307
Hpy188III TCNNGA 3 cut(s) 224, 249, 404
Hpy8I GTNNAC 2 cut(s) 178, 447
Hpy99I CGWCG 2 cut(s) 290, 347
HpyAV CCTTC 4 cut(s) 92, 171, 322, 339
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 4 cut(s) 373, 387, 427, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 424
HpySE526I ACGT 1 cut(s) 436
Hsp92I GRCGYC 2 cut(s) 288, 345
Hsp92II CATG 2 cut(s) 377, 467
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 4 cut(s) 97, 220, 366, 398
LmnI GCTCC 2 cut(s) 83, 150
LpnPI CCDG 9 cut(s) 57, 88, 164, 194, 211, 221, 246, 381, 394
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 436
MalI GATC 4 cut(s) 99, 222, 368, 400
MboI GATC 4 cut(s) 97, 220, 366, 398
MboII GAAGA 5 cut(s) 40, 77, 132, 208, 307
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 449
MlsI TGGCCA 2 cut(s) 239, 264
MluCI AATT 3 cut(s) 227, 252, 455
MluNI TGGCCA 2 cut(s) 239, 264
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 309
Mox20I TGGCCA 2 cut(s) 239, 264
MscI TGGCCA 2 cut(s) 239, 264
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 2 cut(s) 239, 264
MspI CCGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 424
NdeII GATC 4 cut(s) 97, 220, 366, 398
NlaIII CATG 2 cut(s) 377, 467
NlaIV GGNNCC 1 cut(s) 8
NmeAIII GCCGAG 2 cut(s) 373, 406
NruI TCGCGA 1 cut(s) 250
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 1 cut(s) 362
PfeI GAWTC 1 cut(s) 281
PflFI GACNNNGTC 1 cut(s) 410
PkrI GCNGC 1 cut(s) 471
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
PspN4I GGNNCC 1 cut(s) 8
PspPI GGNCC 1 cut(s) 183
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 410
RruI TCGCGA 1 cut(s) 250
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
RseI CAYNNNNRTG 1 cut(s) 362
SatI GCNGC 1 cut(s) 470
Sau3AI GATC 4 cut(s) 97, 220, 366, 398
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 118
SduI GDGCHC 1 cut(s) 449
SetI ASST 6 cut(s) 298, 314, 420, 426, 439, 496
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
Sse9I AATT 3 cut(s) 227, 252, 455
SsiI CCGC 4 cut(s) 148, 362, 469, 519
SspMI CTAG 1 cut(s) 510
TaiI ACGT 1 cut(s) 439
TaqI TCGA 3 cut(s) 219, 225, 414
TasI AATT 3 cut(s) 227, 252, 455
TauI GCSGC 1 cut(s) 472
TfiI GAWTC 1 cut(s) 281
TscAI CASTG 2 cut(s) 162, 454
TspRI CASTG 2 cut(s) 162, 454
Tth111I GACNNNGTC 1 cut(s) 410
VneI GTGCAC 1 cut(s) 445
XapI RAATTY 2 cut(s) 227, 252
XceI RCATGY 1 cut(s) 377
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.