pycom05g05500

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
6651137 .. 6652016
880 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGAGAGAGGAAGCCGATGAAGCAATGTTATATGTTGGTGAAGTTACTTTCCCTGCAAAAAATTACAGCAAAATAACCCACCCATGTAGAAGCTCCCTTTGGATCTTCCATACATGGGCTGACCAATCAACCCAAAGGCCCAAATGGCCCGCAACAAAAACAAAAACTTGCTACACGACGTCGGTACTGGATACACCCCTCCCATCTCTTCCGCATTCACTCACCCTCCCCAAAACCCTCCTCCCTCCCTCTCCCGCCGTCATCCGATCCAGTGGTTCGTGCGGACTCCACAACCAAGTTTCGCTTCCAGATTGCACTCCACCTGTTTATGTAAGTGCATCTGACAACTCGAGTTTTCTAAATTTCAGGCGCCGAAGAAGGACAAGGCAACTCCGCCGTCGTCGAAGCCGGCCAAGTCCGGAGAAGGTCAACAACATGGTGCTCTTCGACCAGGGAAACTACGACAAGTTCCTGACTGAGGCGCCCAAGTACAAGTTGATCAGGCCGTCCATTCTCTCCGACCGCCTCAGGATCAACGGGTTGCTCGCGTGGAGGACGATCAGGGAGTTGATGGCGCGAGGGTTGATTCGGATGGTCTCGGCCCACGCCAGCCAGCAGACCTACGCCAGAGCCACCAACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S25

Protein Analysis

215

Amino Acids

24.47

Weight (kDa)

10.45

Isoelectric Point (pI)

67.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S25 PF03297 139 - 210 6.3e-22 S25 ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 182
AccB1I GGYRCC 2 cut(s) 369, 481
AccII CGCG 2 cut(s) 548, 577
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 6 cut(s) 150, 212, 255, 282, 394, 523
AclWI GGATC 3 cut(s) 110, 261, 539
AcoI YGGCCR 1 cut(s) 410
AcsI RAATTY 1 cut(s) 361
AcyI GRCGYC 3 cut(s) 179, 370, 482
AfaI GTAC 2 cut(s) 186, 491
AfiI CCNNNNNNNGG 2 cut(s) 115, 478
AjnI CCWGG 1 cut(s) 450
AjuI GAANNNNNNNTTGG 4 cut(s) 82, 114, 288, 320
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 601
AlwI GGATC 3 cut(s) 110, 261, 539
Ama87I CYCGRG 1 cut(s) 349
Aor13HI TCCGGA 1 cut(s) 418
AoxI GGCC 5 cut(s) 137, 146, 410, 503, 600
ApoI RAATTY 1 cut(s) 361
AspLEI GCGC 3 cut(s) 372, 484, 577
AspS9I GGNCC 3 cut(s) 138, 147, 601
AsuHPI GGTGA 2 cut(s) 50, 214
AvaI CYCGRG 1 cut(s) 349
AxyI CCTNAGG 1 cut(s) 527
BanI GGYRCC 2 cut(s) 369, 481
Bbv12I GWGCWC 1 cut(s) 444
BccI CCATC 3 cut(s) 211, 565, 586
BceAI ACGGC 3 cut(s) 242, 381, 490
BciT130I CCWGG 1 cut(s) 452
BciVI GTATCC 1 cut(s) 184
BclI TGATCA 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 1 cut(s) 643
BfoI RGCGCY 2 cut(s) 373, 485
BfuI GTATCC 1 cut(s) 184
BglI GCCNNNNNGGC 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 452
BmeT110I CYCGRG 1 cut(s) 349
BmgT120I GGNCC 3 cut(s) 138, 147, 601
BmiI GGNNCC 2 cut(s) 371, 483
BmrFI CCNGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 347
BsaHI GRCGYC 3 cut(s) 179, 370, 482
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 418
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 478
Bse118I RCCGGY 1 cut(s) 408
Bse1I ACTGG 2 cut(s) 192, 270
Bse21I CCTNAGG 1 cut(s) 527
Bse3DI GCAATG 1 cut(s) 30
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 2 cut(s) 261, 597
BseLI CCNNNNNNNGG 2 cut(s) 115, 478
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 468, 541
BseNI ACTGG 2 cut(s) 192, 270
BseRI GAGGAG 1 cut(s) 230
Bsh1236I CGCG 2 cut(s) 548, 577
Bsh1285I CGRYCG 1 cut(s) 523
BshFI GGCC 5 cut(s) 139, 148, 412, 505, 602
BshNI GGYRCC 2 cut(s) 369, 481
BsiEI CGRYCG 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 2 cut(s) 409, 419
BslI CCNNNNNNNGG 2 cut(s) 115, 478
BsmAI GTCTC 1 cut(s) 601
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 5 cut(s) 139, 148, 412, 505, 602
Bso31I GGTCTC 1 cut(s) 601
BsoBI CYCGRG 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 444
Bsp13I TCCGGA 1 cut(s) 418
Bsp143I GATC 5 cut(s) 102, 266, 498, 531, 558
BspACI CCGC 6 cut(s) 150, 212, 255, 282, 394, 523
BspANI GGCC 5 cut(s) 139, 148, 412, 505, 602
BspCNI CTCAG 2 cut(s) 469, 540
BspEI TCCGGA 1 cut(s) 418
BspFNI CGCG 2 cut(s) 548, 577
BspLI GGNNCC 2 cut(s) 371, 483
BspPI GGATC 3 cut(s) 110, 261, 539
BspQI GCTCTTC 1 cut(s) 449
BspT107I GGYRCC 2 cut(s) 369, 481
BspTNI GGTCTC 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 30
BsrFI RCCGGY 1 cut(s) 408
BsrI ACTGG 2 cut(s) 192, 270
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 5 cut(s) 102, 266, 498, 531, 558
BssNI GRCGYC 3 cut(s) 179, 370, 482
Bst2UI CCWGG 1 cut(s) 452
Bst6I CTCTTC 2 cut(s) 213, 449
BstACI GRCGYC 3 cut(s) 179, 370, 482
BstC8I GCNNGC 5 cut(s) 150, 410, 546, 610, 614
BstDEI CTNAG 2 cut(s) 477, 527
BstENI CCTNNNNNAGG 1 cut(s) 476
BstF5I GGATG 2 cut(s) 261, 597
BstFNI CGCG 2 cut(s) 548, 577
BstH2I RGCGCY 2 cut(s) 373, 485
BstHHI GCGC 3 cut(s) 372, 484, 577
BstKTI GATC 5 cut(s) 105, 269, 501, 534, 561
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 5 cut(s) 102, 266, 498, 531, 558
BstMCI CGRYCG 1 cut(s) 523
BstMWI GCNNNNNNNGC 2 cut(s) 20, 145
BstNI CCWGG 1 cut(s) 452
BstSCI CCNGG 1 cut(s) 450
BstUI CGCG 2 cut(s) 548, 577
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
Bsu36I CCTNAGG 1 cut(s) 527
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 5 cut(s) 139, 148, 412, 505, 602
BtsCI GGATG 2 cut(s) 261, 597
BtsIMutI CAGTG 1 cut(s) 277
Cac8I GCNNGC 5 cut(s) 150, 410, 546, 610, 614
CfoI GCGC 3 cut(s) 372, 484, 577
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 3 cut(s) 138, 147, 601
Csp6I GTAC 2 cut(s) 185, 490
CviAII CATG 3 cut(s) 84, 114, 436
CviQI GTAC 2 cut(s) 185, 490
DdeI CTNAG 2 cut(s) 477, 527
DinI GGCGCC 2 cut(s) 371, 483
DpnI GATC 5 cut(s) 104, 268, 500, 533, 560
DpnII GATC 5 cut(s) 102, 266, 498, 531, 558
EaeI YGGCCR 1 cut(s) 410
Eam1104I CTCTTC 2 cut(s) 213, 449
EarI CTCTTC 2 cut(s) 213, 449
EciI GGCGGA 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 601
Eco81I CCTNAGG 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 450
EgeI GGCGCC 2 cut(s) 371, 483
EheI GGCGCC 2 cut(s) 371, 483
FaeI CATG 3 cut(s) 87, 117, 439
FaiI YATR 7 cut(s) 31, 33, 85, 111, 115, 330, 437
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FatI CATG 3 cut(s) 83, 113, 435
FauI CCCGC 2 cut(s) 157, 262
FbaI TGATCA 1 cut(s) 498
FokI GGATG 2 cut(s) 248, 604
FspBI CTAG 1 cut(s) 643
GlaI GCGC 3 cut(s) 371, 483, 576
HaeII RGCGCY 2 cut(s) 373, 485
HaeIII GGCC 5 cut(s) 139, 148, 412, 505, 602
HapII CCGG 2 cut(s) 409, 419
HhaI GCGC 3 cut(s) 372, 484, 577
Hin1I GRCGYC 3 cut(s) 179, 370, 482
Hin1II CATG 3 cut(s) 87, 117, 439
Hin6I GCGC 3 cut(s) 370, 482, 575
HinP1I GCGC 3 cut(s) 370, 482, 575
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 2 cut(s) 285, 586
HpaII CCGG 2 cut(s) 409, 419
HphI GGTGA 2 cut(s) 50, 214
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 4 cut(s) 266, 343, 520, 591
Hpy188III TCNNGA 4 cut(s) 308, 419, 472, 529
Hpy8I GTNNAC 1 cut(s) 430
Hpy99I CGWCG 4 cut(s) 181, 184, 402, 405
HpyAV CCTTC 2 cut(s) 372, 418
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 3 cut(s) 56, 315, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 145
HpyF3I CTNAG 2 cut(s) 477, 527
HpySE526I ACGT 1 cut(s) 179
Hsp92I GRCGYC 3 cut(s) 179, 370, 482
Hsp92II CATG 3 cut(s) 87, 117, 439
HspAI GCGC 3 cut(s) 370, 482, 575
KasI GGCGCC 2 cut(s) 369, 481
Kpn2I TCCGGA 1 cut(s) 418
KroI GCCGGC 1 cut(s) 408
KroNI GCCGGC 1 cut(s) 410
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 5 cut(s) 102, 266, 498, 531, 558
LguI GCTCTTC 1 cut(s) 449
LmnI GCTCC 1 cut(s) 98
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 643
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 43
MalI GATC 5 cut(s) 104, 268, 500, 533, 560
MboI GATC 5 cut(s) 102, 266, 498, 531, 558
MboII GAAGA 4 cut(s) 97, 200, 387, 436
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 2 cut(s) 61, 361
Mly113I GGCGCC 2 cut(s) 370, 482
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 1 cut(s) 543
MroI TCCGGA 1 cut(s) 418
MroNI GCCGGC 1 cut(s) 408
MspI CCGG 2 cut(s) 409, 419
MspR9I CCNGG 1 cut(s) 452
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 1 cut(s) 452
MvnI CGCG 2 cut(s) 548, 577
MwoI GCNNNNNNNGC 2 cut(s) 20, 145
NaeI GCCGGC 1 cut(s) 410
NarI GGCGCC 2 cut(s) 370, 482
NdeII GATC 5 cut(s) 102, 266, 498, 531, 558
NgoMIV GCCGGC 1 cut(s) 408
NlaIII CATG 3 cut(s) 87, 117, 439
NlaIV GGNNCC 2 cut(s) 371, 483
NmeAIII GCCGAG 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 449
PctI GAATGC 1 cut(s) 214
PdiI GCCGGC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 586
PleI GAGTC 1 cut(s) 279
PluTI GGCGCC 2 cut(s) 373, 485
PpsI GAGTC 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 450
PspGI CCWGG 1 cut(s) 450
PspN4I GGNNCC 2 cut(s) 371, 483
PspPI GGNCC 3 cut(s) 138, 147, 601
PspXI VCTCGAGB 1 cut(s) 349
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 2 cut(s) 186, 491
RsaNI GTAC 2 cut(s) 185, 490
SapI GCTCTTC 1 cut(s) 449
Sau3AI GATC 5 cut(s) 102, 266, 498, 531, 558
Sau96I GGNCC 3 cut(s) 138, 147, 601
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 452
SduI GDGCHC 1 cut(s) 444
SetI ASST 6 cut(s) 95, 182, 325, 429, 623, 644
SfaNI GCATC 1 cut(s) 347
SfiI GGCCNNNNNGGCC 1 cut(s) 145
SfoI GGCGCC 2 cut(s) 371, 483
Sfr274I CTCGAG 1 cut(s) 349
SlaI CTCGAG 1 cut(s) 349
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 2 cut(s) 61, 361
SsiI CCGC 6 cut(s) 150, 212, 255, 282, 394, 523
SspDI GGCGCC 2 cut(s) 369, 481
SspMI CTAG 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 450
TaiI ACGT 1 cut(s) 182
TaqI TCGA 3 cut(s) 350, 403, 447
TasI AATT 2 cut(s) 61, 361
TatI WGTACW 1 cut(s) 489
TfiI GAWTC 1 cut(s) 586
TscAI CASTG 1 cut(s) 277
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 476
XapI RAATTY 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 349
XspI CTAG 1 cut(s) 643
ZraI GACGTC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.