pycom05g07790

LisH domain and HEAT repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
10621888 .. 10622918
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGATTCAATCGGATCGGTACACTTCATTCCCTTTTTCCATAATGAAGTCTGAAACATCTAGGTGGGTTGTGTTCAACTGCCCTGATAAGTTGAGCACTGGGTTAGGTATATCTATTGGTCTAGCCTGGCTTGATATAGATCCACCCGTTGTTTTGGGGTACGATAGGTACGCGCCCAATGCCCATTGCCACCACACCTATGACATGATTCTTCAGAGCTTGGGCCTAAATTATGCCTTAGAATCTGGTTATGAAATTGGACACCATGGTTCTTGCCTTTCCTGTTCCACTTGCCTCTCTTGTGGCCACGCCCTAGTTTGCAATTATTGCCATGAGAGGATCTGGTATTCCTTTCAAGGGAAGCAACATTCACTTCTGGGAATGATATATCAGCTGGTTGTATTCAATGAAGCCTCACTCTTTATACTGCTACTGCAAGAGCACGTTGGAGGCATGAAATGTGCTGAAAGTCTTTTCCAGCATATCTTCCTCATTAATGGCTTCCCCATAAAGCTTCATCTGGGAGTTAATTCTGAACATCGCAAAATTTTCACTCATAACGAGCTTTTGGAAGAATCATCGTTTTCTGGTGATTGTATCTGTTTCTCAATGCCTTCCAGAGAGCTAATGGATCTTCAACTGTTAGGTACTCGCTCTTTAGTCCGTCATCAATGTGGATGCACTGTTTTCCTCCCTAATGGTTTTTACAAGATTTCCTGTCTCCAGATCGATCTTGGTATCCACTACCGAAGTAAGGTAGTTCTTCCTAGTAATGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.23

Weight (kDa)

6.41

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 173
AclWI GGATC 4 cut(s) 21, 134, 347, 639
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 546
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 4 cut(s) 20, 161, 170, 649
AfiI CCNNNNNNNGG 2 cut(s) 357, 754
AgsI TTSAA 5 cut(s) 8, 76, 356, 406, 638
AjnI CCWGG 1 cut(s) 125
AluBI AGCT 5 cut(s) 219, 394, 514, 565, 625
AluI AGCT 5 cut(s) 219, 394, 514, 565, 625
Alw21I GWGCWC 2 cut(s) 98, 444
Alw26I GTCTC 1 cut(s) 725
AlwI GGATC 4 cut(s) 21, 134, 347, 639
AoxI GGCC 2 cut(s) 223, 304
ApoI RAATTY 1 cut(s) 546
AseI ATTAAT 1 cut(s) 495
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 602
BalI TGGCCA 1 cut(s) 306
Bbv12I GWGCWC 2 cut(s) 98, 444
BciT130I CCWGG 1 cut(s) 127
BciVI GTATCC 1 cut(s) 749
BcoDI GTCTC 1 cut(s) 725
BfaI CTAG 4 cut(s) 60, 122, 314, 768
BfuI GTATCC 1 cut(s) 749
Bme1390I CCNGG 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 127
BmrI ACTGGG 1 cut(s) 108
BmsI GCATC 1 cut(s) 668
BmuI ACTGGG 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 707
Bsa29I ATCGAT 1 cut(s) 729
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 357, 754
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 184
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 127
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 683
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 2 cut(s) 357, 754
BseMI GCAATG 1 cut(s) 184
BseNI ACTGG 1 cut(s) 103
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 2 cut(s) 225, 306
BshVI ATCGAT 1 cut(s) 729
BsiHKAI GWGCWC 2 cut(s) 98, 444
BslI CCNNNNNNNGG 2 cut(s) 357, 754
BsmAI GTCTC 1 cut(s) 725
BsnI GGCC 2 cut(s) 225, 306
Bsp1286I GDGCHC 2 cut(s) 98, 444
Bsp143I GATC 6 cut(s) 13, 139, 339, 631, 726, 730
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 2 cut(s) 225, 306
BspDI ATCGAT 1 cut(s) 729
BspFNI CGCG 1 cut(s) 173
BspPI GGATC 4 cut(s) 21, 134, 347, 639
BsrDI GCAATG 1 cut(s) 184
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 6 cut(s) 13, 139, 339, 631, 726, 730
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 642, 685
BstAPI GCANNNNNTGC 1 cut(s) 327
BstDEI CTNAG 1 cut(s) 238
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 683
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 6 cut(s) 16, 142, 342, 634, 729, 733
BstMAI GTCTC 1 cut(s) 725
BstMBI GATC 6 cut(s) 13, 139, 339, 631, 726, 730
BstMWI GCNNNNNNNGC 2 cut(s) 179, 327
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 125
BstUI CGCG 1 cut(s) 173
BstX2I RGATCY 3 cut(s) 139, 339, 631
BstYI RGATCY 3 cut(s) 139, 339, 631
Bsu15I ATCGAT 1 cut(s) 729
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 2 cut(s) 225, 306
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 524
BtsCI GGATG 1 cut(s) 683
BtsIMutI CAGTG 2 cut(s) 96, 681
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 223
ClaI ATCGAT 1 cut(s) 729
Csp6I GTAC 4 cut(s) 19, 160, 169, 648
CviAII CATG 4 cut(s) 205, 266, 332, 454
CviQI GTAC 4 cut(s) 19, 160, 169, 648
DdeI CTNAG 1 cut(s) 238
DpnI GATC 6 cut(s) 15, 141, 341, 633, 728, 732
DpnII GATC 6 cut(s) 13, 139, 339, 631, 726, 730
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 4 cut(s) 208, 269, 335, 457
FatI CATG 4 cut(s) 204, 265, 331, 453
FokI GGATG 1 cut(s) 690
FspBI CTAG 4 cut(s) 60, 122, 314, 768
GlaI GCGC 1 cut(s) 174
GsuI CTGGAG 1 cut(s) 707
HaeIII GGCC 2 cut(s) 225, 306
HhaI GCGC 1 cut(s) 175
Hin1II CATG 4 cut(s) 208, 269, 335, 457
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HindIII AAGCTT 1 cut(s) 512
HinfI GANTC 4 cut(s) 4, 208, 242, 575
HphI GGTGA 1 cut(s) 602
Hpy166II GTNNAC 1 cut(s) 21
Hpy188I TCNGA 4 cut(s) 13, 52, 216, 535
Hpy188III TCNNGA 2 cut(s) 618, 724
Hpy8I GTNNAC 1 cut(s) 21
HpyAV CCTTC 1 cut(s) 624
HpyCH4III ACNGT 2 cut(s) 642, 685
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 3 cut(s) 321, 436, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 327
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 4 cut(s) 208, 269, 335, 457
HspAI GCGC 1 cut(s) 173
Kzo9I GATC 6 cut(s) 13, 139, 339, 631, 726, 730
LweI GCATC 1 cut(s) 668
MaeI CTAG 4 cut(s) 60, 122, 314, 768
MaeII ACGT 1 cut(s) 444
MalI GATC 6 cut(s) 15, 141, 341, 633, 728, 732
MboI GATC 6 cut(s) 13, 139, 339, 631, 726, 730
MboII GAAGA 5 cut(s) 203, 478, 584, 626, 755
MflI RGATCY 3 cut(s) 139, 339, 631
MhlI GDGCHC 2 cut(s) 98, 444
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 5 cut(s) 229, 255, 322, 529, 546
MluNI TGGCCA 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 6 cut(s) 305, 330, 424, 443, 500, 701
Mox20I TGGCCA 1 cut(s) 306
MscI TGGCCA 1 cut(s) 306
MseI TTAA 2 cut(s) 495, 528
MslI CAYNNNNRTG 3 cut(s) 61, 198, 672
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 394
MspR9I CCNGG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 179, 327
NcoI CCATGG 1 cut(s) 265
NdeII GATC 6 cut(s) 13, 139, 339, 631, 726, 730
NlaIII CATG 4 cut(s) 208, 269, 335, 457
PfeI GAWTC 4 cut(s) 4, 208, 242, 575
PshBI ATTAAT 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 125
PspGI CCWGG 1 cut(s) 125
PspPI GGNCC 1 cut(s) 223
PsuI RGATCY 3 cut(s) 139, 339, 631
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 4 cut(s) 20, 161, 170, 649
RsaNI GTAC 4 cut(s) 19, 160, 169, 648
RseI CAYNNNNRTG 3 cut(s) 61, 198, 672
SaqAI TTAA 2 cut(s) 495, 528
Sau3AI GATC 6 cut(s) 13, 139, 339, 631, 726, 730
Sau96I GGNCC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 127
SduI GDGCHC 2 cut(s) 98, 444
SfaNI GCATC 1 cut(s) 668
SmiMI CAYNNNNRTG 3 cut(s) 61, 198, 672
Sse9I AATT 5 cut(s) 229, 255, 322, 529, 546
SspMI CTAG 4 cut(s) 60, 122, 314, 768
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 642, 685
TaiI ACGT 1 cut(s) 447
TaqI TCGA 1 cut(s) 729
TasI AATT 5 cut(s) 229, 255, 322, 529, 546
TfiI GAWTC 4 cut(s) 4, 208, 242, 575
Tru1I TTAA 2 cut(s) 495, 528
Tru9I TTAA 2 cut(s) 495, 528
TscAI CASTG 2 cut(s) 103, 688
TspDTI ATGAA 6 cut(s) 15, 59, 267, 423, 470, 506
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 2 cut(s) 103, 688
VspI ATTAAT 1 cut(s) 495
XapI RAATTY 1 cut(s) 546
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 731
XspI CTAG 4 cut(s) 60, 122, 314, 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.