pycom05g11830

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
15398718 .. 15399680
963 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 963 bp
ATGGGGGGATGTGTTGCTTCCAAGCTGGAGGAAGAAGGAGGAGTTGTGACAATTTGTAGAGAGAGAAAGCGGCAGCTAAAGCTGGCGGTGCAGAAAAGATACGCCCTTGCGGAGGCGCACTGTAAGTACGGCCATGCTTTGTTTGCGGTGGCTGCCGCCGTGGACTTCTTTGTTGCAGGCTACTCTTCCCCTCCTAAACCTTACCTCATCACATTTTCGCCTGACTCTCCTCCTCAGCCGCCGGAGTCTGCGGAGTCGGCTCCGCTTCCGATCACTGAGAATGTGATAACCAACCCAATGTTTCTTCAGCAGAGACCGTCTGAGTCGACCCATGAAGCCATTGCTTGTCACTCATGTGGTTCTTCAACATCCTCCGACTCCTCTGAGGAGGAGAGAGAAGGAGAGGAGCGAAAAACAGACGTGAGAGAAGAACAGGGATGTGGGTACTTTTATATGCAAATGCCACCAGCAATGCCGTCACCGCAGACAGACTTTGGGTGGGATTTCTTCAACCCTTTCGACGTCGTGAGGCCGGAGGTCATAAGCGGGTACAGGCGGAGCTCCGACGAGGATTTGAGGGTGGTGAGGGAGGAGGAAGGGATACCTGAGCTGGAAGAGGAAGAAGGGGAGAGTCAGAGACAAGAAGAGCAGGAAGAAAACAAGGCGGTGGTGGTGGAAGAAAAAGGAAGCGGAGAACAGCGTGACGGCGGTGCTGAGGTGGTGAAGGTGGTTGAAGAGGCGGCTAATGCGAGGGAGGGGGAGCAAAAAGGGCTTGCTGTGATTAATACACCAGCTGAAGGGAGGGAGCTTCTGGAAGCACTGAAGGACATTGTGGACCTTCTCTCTAGGGCTTCTGATTCTGCAAAGGATGTGTCCAGGATGCTTGACGCTAATAAATTGCAAATGCAGTCTGGTTTGGAGGAAATTAAAGGTGGGTTCTTTTCAAAACTTGAAAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

321

Amino Acids

35.35

Weight (kDa)

4.59

Isoelectric Point (pI)

71.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF630 PF04783 3 - 59 3.3e-17 Protein of unknown function (DUF630)
DUF632 PF04782 270 - 311 5.2e-06 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 525
AccI GTMKAC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 1 cut(s) 955
AcuI CTGAAG 3 cut(s) 290, 816, 842
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 3 cut(s) 128, 446, 551
AfiI CCNNNNNNNGG 2 cut(s) 112, 797
AgsI TTSAA 5 cut(s) 366, 511, 734, 945, 953
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 1 cut(s) 875
AleI CACNNNNGTG 1 cut(s) 354
AluBI AGCT 7 cut(s) 25, 76, 82, 561, 610, 794, 808
AluI AGCT 7 cut(s) 25, 76, 82, 561, 610, 794, 808
Alw21I GWGCWC 1 cut(s) 563
Alw26I GTCTC 2 cut(s) 307, 631
AoxI GGCC 2 cut(s) 130, 530
ApeKI GCWGC 2 cut(s) 73, 152
ApoI RAATTY 1 cut(s) 955
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
AseI ATTAAT 1 cut(s) 783
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 835
AsuHPI GGTGA 3 cut(s) 471, 595, 733
AvaII GGWCC 1 cut(s) 835
BanII GRGCYC 1 cut(s) 563
Bbv12I GWGCWC 1 cut(s) 563
BbvCI CCTCAGC 2 cut(s) 234, 714
BbvI GCAGC 2 cut(s) 85, 139
BceAI ACGGC 4 cut(s) 143, 145, 460, 721
BciT130I CCWGG 1 cut(s) 877
BciVI GTATCC 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 307, 631
BfaI CTAG 1 cut(s) 846
BfuI GTATCC 1 cut(s) 594
BisI GCNGC 6 cut(s) 71, 74, 153, 156, 239, 741
BlsI GCNGC 6 cut(s) 72, 75, 154, 157, 240, 742
Bme1390I CCNGG 1 cut(s) 877
Bme18I GGWCC 1 cut(s) 835
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 835
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 877
BmsI GCATC 1 cut(s) 870
BpmI CTGGAG 1 cut(s) 47
Bpu10I CCTNAGC 3 cut(s) 234, 606, 714
BsaHI GRCGYC 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 159
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 797
Bse3DI GCAATG 2 cut(s) 339, 477
BseBI CCWGG 1 cut(s) 877
BseDI CCNNGG 1 cut(s) 159
BseGI GGATG 5 cut(s) 14, 368, 443, 874, 885
BseLI CCNNNNNNNGG 2 cut(s) 112, 797
BseMI GCAATG 2 cut(s) 339, 477
BseMII CTCAG 6 cut(s) 248, 267, 312, 375, 597, 705
BseRI GAGGAG 8 cut(s) 54, 219, 222, 370, 401, 404, 419, 605
BseXI GCAGC 2 cut(s) 85, 139
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 2 cut(s) 132, 532
BsiHKAI GWGCWC 1 cut(s) 563
BsiSI CCGG 2 cut(s) 242, 533
BslI CCNNNNNNNGG 2 cut(s) 112, 797
BsmAI GTCTC 2 cut(s) 307, 631
BsnI GGCC 2 cut(s) 132, 532
Bso31I GGTCTC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 563
Bsp143I GATC 1 cut(s) 270
BspANI GGCC 2 cut(s) 132, 532
BspCNI CTCAG 6 cut(s) 247, 268, 313, 376, 598, 706
BspLI GGNNCC 1 cut(s) 261
BspQI GCTCTTC 1 cut(s) 639
BspTNI GGTCTC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 339, 477
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 270
BssNI GRCGYC 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 877
Bst4CI ACNGT 2 cut(s) 122, 318
Bst6I CTCTTC 4 cut(s) 190, 609, 639, 729
BstACI GRCGYC 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 84, 178, 774
BstDEI CTNAG 6 cut(s) 234, 276, 321, 384, 606, 714
BstDSI CCRYGG 1 cut(s) 159
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 5 cut(s) 14, 368, 443, 874, 885
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 1 cut(s) 273
BstMAI GTCTC 2 cut(s) 307, 631
BstMBI GATC 1 cut(s) 270
BstMWI GCNNNNNNNGC 8 cut(s) 79, 88, 143, 152, 257, 481, 746, 769
BstNI CCWGG 1 cut(s) 877
BstSCI CCNGG 1 cut(s) 875
BstV1I GCAGC 2 cut(s) 85, 139
BsuI GTATCC 1 cut(s) 594
BsuRI GGCC 2 cut(s) 132, 532
BtgI CCRYGG 1 cut(s) 159
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 5 cut(s) 14, 368, 443, 874, 885
BtsIMutI CAGTG 3 cut(s) 118, 273, 818
Cac8I GCNNGC 3 cut(s) 84, 178, 774
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 835
CseI GACGC 1 cut(s) 896
Csp6I GTAC 3 cut(s) 127, 445, 550
CviAII CATG 3 cut(s) 134, 332, 354
CviQI GTAC 3 cut(s) 127, 445, 550
DdeI CTNAG 6 cut(s) 234, 276, 321, 384, 606, 714
DpnI GATC 1 cut(s) 272
DpnII GATC 1 cut(s) 270
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 4 cut(s) 190, 609, 639, 729
EarI CTCTTC 4 cut(s) 190, 609, 639, 729
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 835
Eco53kI GAGCTC 1 cut(s) 561
Eco57I CTGAAG 3 cut(s) 290, 816, 842
EcoICRI GAGCTC 1 cut(s) 561
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 875
EcoT38I GRGCYC 1 cut(s) 563
FaeI CATG 3 cut(s) 137, 335, 357
FaiI YATR 6 cut(s) 135, 333, 355, 453, 455, 542
FatI CATG 3 cut(s) 133, 331, 353
FauI CCCGC 1 cut(s) 539
FblI GTMKAC 1 cut(s) 326
Fnu4HI GCNGC 6 cut(s) 71, 74, 153, 156, 239, 741
FokI GGATG 5 cut(s) 21, 355, 450, 881, 892
FriOI GRGCYC 1 cut(s) 563
Fsp4HI GCNGC 6 cut(s) 71, 74, 153, 156, 239, 741
FspBI CTAG 1 cut(s) 846
GlaI GCGC 1 cut(s) 117
GluI GCNGC 6 cut(s) 71, 74, 153, 156, 239, 741
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 2 cut(s) 132, 532
HapII CCGG 2 cut(s) 242, 533
HgaI GACGC 1 cut(s) 896
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 3 cut(s) 137, 335, 357
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HinfI GANTC 7 cut(s) 224, 245, 254, 323, 377, 631, 857
HpaII CCGG 2 cut(s) 242, 533
HphI GGTGA 3 cut(s) 471, 595, 733
Hpy166II GTNNAC 3 cut(s) 163, 327, 835
Hpy188I TCNGA 7 cut(s) 270, 322, 376, 385, 565, 636, 856
Hpy188III TCNNGA 2 cut(s) 526, 812
Hpy8I GTNNAC 3 cut(s) 163, 327, 835
Hpy99I CGWCG 3 cut(s) 524, 527, 569
HpyAV CCTTC 8 cut(s) 29, 392, 590, 617, 718, 791, 817, 848
HpyCH4III ACNGT 2 cut(s) 122, 318
HpyCH4IV ACGT 2 cut(s) 420, 522
HpyCH4V TGCA 6 cut(s) 91, 176, 457, 863, 901, 907
HpyF10VI GCNNNNNNNGC 8 cut(s) 79, 88, 143, 152, 257, 481, 746, 769
HpyF3I CTNAG 6 cut(s) 234, 276, 321, 384, 606, 714
HpySE526I ACGT 2 cut(s) 420, 522
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 3 cut(s) 137, 335, 357
HspAI GCGC 1 cut(s) 116
Kzo9I GATC 1 cut(s) 270
LguI GCTCTTC 1 cut(s) 639
LmnI GCTCC 6 cut(s) 265, 406, 558, 566, 760, 805
Lsp1109I GCAGC 2 cut(s) 85, 139
LweI GCATC 1 cut(s) 870
MaeI CTAG 1 cut(s) 846
MaeII ACGT 2 cut(s) 420, 522
MaeIII GTNAC 4 cut(s) 46, 347, 477, 701
MalI GATC 1 cut(s) 272
MboI GATC 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 563
MluCI AATT 4 cut(s) 51, 896, 924, 955
MlyI GAGTC 6 cut(s) 218, 254, 263, 332, 371, 640
MmeI TCCRAC 2 cut(s) 399, 588
MseI TTAA 3 cut(s) 783, 927, 961
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 2 cut(s) 242, 533
MspR9I CCNGG 1 cut(s) 877
MvaI CCWGG 1 cut(s) 877
MwoI GCNNNNNNNGC 8 cut(s) 79, 88, 143, 152, 257, 481, 746, 769
NdeII GATC 1 cut(s) 270
NlaIII CATG 3 cut(s) 137, 335, 357
NlaIV GGNNCC 1 cut(s) 261
NmuCI GTSAC 4 cut(s) 46, 347, 477, 701
OliI CACNNNNGTG 1 cut(s) 354
PciSI GCTCTTC 1 cut(s) 639
PfeI GAWTC 1 cut(s) 857
PfoI TCCNGGA 1 cut(s) 875
PkrI GCNGC 6 cut(s) 72, 75, 154, 157, 240, 742
PleI GAGTC 6 cut(s) 218, 253, 262, 331, 371, 639
PpsI GAGTC 6 cut(s) 218, 253, 262, 331, 371, 639
PshBI ATTAAT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 875
PspGI CCWGG 1 cut(s) 875
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 835
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 3 cut(s) 128, 446, 551
RsaNI GTAC 3 cut(s) 127, 445, 550
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 563
SalI GTCGAC 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 3 cut(s) 783, 927, 961
SatI GCNGC 6 cut(s) 71, 74, 153, 156, 239, 741
Sau3AI GATC 1 cut(s) 270
Sau96I GGNCC 1 cut(s) 835
SchI GAGTC 6 cut(s) 218, 254, 263, 332, 371, 640
ScrFI CCNGG 1 cut(s) 877
SduI GDGCHC 1 cut(s) 563
SfaNI GCATC 1 cut(s) 870
SinI GGWCC 1 cut(s) 835
SmiMI CAYNNNNRTG 1 cut(s) 354
Sse9I AATT 4 cut(s) 51, 896, 924, 955
SspMI CTAG 1 cut(s) 846
SstI GAGCTC 1 cut(s) 563
StyD4I CCNGG 1 cut(s) 875
TaaI ACNGT 2 cut(s) 122, 318
TaiI ACGT 2 cut(s) 423, 525
TaqI TCGA 2 cut(s) 326, 519
TasI AATT 4 cut(s) 51, 896, 924, 955
TauI GCSGC 4 cut(s) 73, 158, 241, 743
TfiI GAWTC 1 cut(s) 857
Tru1I TTAA 3 cut(s) 783, 927, 961
Tru9I TTAA 3 cut(s) 783, 927, 961
TscAI CASTG 3 cut(s) 125, 280, 825
TseFI GTSAC 4 cut(s) 46, 347, 477, 701
TseI GCWGC 2 cut(s) 73, 152
Tsp45I GTSAC 4 cut(s) 46, 347, 477, 701
TspDTI ATGAA 1 cut(s) 348
TspRI CASTG 3 cut(s) 125, 280, 825
VpaK11BI GGWCC 1 cut(s) 835
VspI ATTAAT 1 cut(s) 783
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 955
XmiI GTMKAC 1 cut(s) 326
XspI CTAG 1 cut(s) 846
ZraI GACGTC 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.