pycom05g14820

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
18368305 .. 18369682
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGATGACCTGGGAATCGAAAATTTCAATGCTGGAAGAGGGGCTTGAGATATCACAGCCAGCACTTGATCCTGACAACTCCGAGGTGCATCATCTTCTTACGGCGAGAGACAACAGAACAGAAGTACACAGGAACATAAACAATGCACTTCGCGGTGGAAGGAAGTGTGATGAAAACAGCACAGATCCTTCATGCACAGGGTTTGTAGAAATGATGGCTCCTGTTAATTCAAGAGCATCCTGGCGCTGCATGTGGCATATTATTCAGAATGACTTAGTTCATGCTTGGGTTTTGGGTTTCCAGCTGGGTTATTGTGCACAGACAATTTCTCCATCTTGTGAATTACATTTTTCTTACTGTTTTTCGCCTGTTCTCTTGTACAAATTTCGTACGTCTGAAGCAATCCTTTACAGGGCGATTGAACCAAAAATATCGGAATTGTTGATTCTGAGTACATTATTCATTAGGTATTCCTTCACTAGGGGGCTTGGCGGCAAACAAGGTCGGAAACTTCATACAACTCCCTCTCTATCCCTGCACGACTCTATGTTGTTAAGTTGTATAATTTGTATGATCAGTTTATCACTCACTAATGGCCACAGTCACCAATGCAGACAATTGCAGAAGCAAATGATCAACCTGCTCACAATAAAAGCTTGCCTAGTTCAGAACAACTGGTATCTGACCCTAGAACCGAGTTGA

Protein Analysis

234

Amino Acids

26.56

Weight (kDa)

7.6

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 12 - 108 1.4e-39 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 648
AccII CGCG 1 cut(s) 153
AciI CCGC 2 cut(s) 153, 492
AclWI GGATC 2 cut(s) 62, 179
AcoI YGGCCR 1 cut(s) 595
AcsI RAATTY 2 cut(s) 21, 383
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 4 cut(s) 126, 380, 391, 454
AfiI CCNNNNNNNGG 2 cut(s) 412, 480
AgsI TTSAA 3 cut(s) 27, 231, 422
AjnI CCWGG 2 cut(s) 8, 239
AluBI AGCT 2 cut(s) 304, 656
AluI AGCT 2 cut(s) 304, 656
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 102
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 2 cut(s) 62, 179
AoxI GGCC 1 cut(s) 595
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 2 cut(s) 21, 383
AspLEI GCGC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 233
BccI CCATC 2 cut(s) 208, 340
BceAI ACGGC 1 cut(s) 117
BciT130I CCWGG 2 cut(s) 10, 241
BclI TGATCA 2 cut(s) 573, 633
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 3 cut(s) 480, 662, 689
BfoI RGCGCY 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 648
BisI GCNGC 2 cut(s) 247, 493
BlsI GCNGC 2 cut(s) 248, 494
Bme1390I CCNGG 2 cut(s) 10, 241
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 10, 241
BmsI GCATC 2 cut(s) 97, 245
BpuEI CTTGAG 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 9, 81
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 480
Bse1I ACTGG 1 cut(s) 680
BseBI CCWGG 2 cut(s) 10, 241
BseDI CCNNGG 2 cut(s) 9, 81
BseGI GGATG 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 412, 480
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 233
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 319
BsiWI CGTACG 1 cut(s) 389
BslI CCNNNNNNNGG 2 cut(s) 412, 480
BsmAI GTCTC 1 cut(s) 102
BsnI GGCC 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 67, 184, 573, 633
BspACI CCGC 2 cut(s) 153, 492
BspANI GGCC 1 cut(s) 597
BspCNI CTCAG 1 cut(s) 441
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 219
BspMI ACCTGC 1 cut(s) 648
BspPI GGATC 2 cut(s) 62, 179
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 680
BssECI CCNNGG 2 cut(s) 9, 81
BssMI GATC 4 cut(s) 67, 184, 573, 633
Bst2UI CCWGG 2 cut(s) 10, 241
Bst4CI ACNGT 2 cut(s) 359, 602
Bst6I CTCTTC 1 cut(s) 30
BstAUI TGTACA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 60, 658
BstDEI CTNAG 2 cut(s) 274, 449
BstENI CCTNNNNNAGG 1 cut(s) 478
BstF5I GGATG 1 cut(s) 236
BstFNI CGCG 1 cut(s) 153
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 4 cut(s) 70, 187, 576, 636
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 4 cut(s) 67, 184, 573, 633
BstNI CCWGG 2 cut(s) 10, 241
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 8, 239
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 1 cut(s) 597
BtsCI GGATG 1 cut(s) 236
BveI ACCTGC 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 60, 658
CfoI GCGC 1 cut(s) 246
Csp6I GTAC 4 cut(s) 125, 379, 390, 453
CviAII CATG 3 cut(s) 192, 250, 281
CviJI RGCY 7 cut(s) 43, 58, 218, 304, 487, 597, 656
CviKI_1 RGCY 7 cut(s) 43, 58, 218, 304, 487, 597, 656
CviQI GTAC 4 cut(s) 125, 379, 390, 453
DdeI CTNAG 2 cut(s) 274, 449
DpnI GATC 4 cut(s) 69, 186, 575, 635
DpnII GATC 4 cut(s) 67, 184, 573, 633
EaeI YGGCCR 1 cut(s) 595
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco32I GATATC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 478
EcoRII CCWGG 2 cut(s) 8, 239
EcoRV GATATC 1 cut(s) 51
FaeI CATG 3 cut(s) 195, 253, 284
FaiI YATR 9 cut(s) 137, 193, 251, 258, 282, 516, 548, 563, 572
FalI AAGNNNNNCTT 4 cut(s) 27, 59, 390, 422
FatI CATG 3 cut(s) 191, 249, 280
FbaI TGATCA 2 cut(s) 573, 633
Fnu4HI GCNGC 2 cut(s) 247, 493
FokI GGATG 1 cut(s) 223
Fsp4HI GCNGC 2 cut(s) 247, 493
FspBI CTAG 3 cut(s) 480, 662, 689
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 247, 493
GsaI CCCAGC 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 1 cut(s) 597
HhaI GCGC 1 cut(s) 246
Hin1II CATG 3 cut(s) 195, 253, 284
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 3 cut(s) 14, 445, 542
HphI GGTGA 1 cut(s) 596
Hpy166II GTNNAC 2 cut(s) 127, 317
Hpy188I TCNGA 8 cut(s) 82, 267, 397, 436, 450, 507, 669, 684
Hpy188III TCNNGA 2 cut(s) 71, 231
Hpy8I GTNNAC 2 cut(s) 127, 317
HpyAV CCTTC 3 cut(s) 153, 198, 484
HpyCH4III ACNGT 2 cut(s) 359, 602
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 8 cut(s) 88, 146, 195, 249, 317, 538, 612, 622
HpyF3I CTNAG 2 cut(s) 274, 449
HpySE526I ACGT 1 cut(s) 392
Hsp92II CATG 3 cut(s) 195, 253, 284
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 2 cut(s) 573, 633
Kzo9I GATC 4 cut(s) 67, 184, 573, 633
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 97, 245
MaeI CTAG 3 cut(s) 480, 662, 689
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 1 cut(s) 602
MalI GATC 4 cut(s) 69, 186, 575, 635
MboI GATC 4 cut(s) 67, 184, 573, 633
MboII GAAGA 2 cut(s) 47, 86
MfeI CAATTG 1 cut(s) 617
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 597
MluCI AATT 8 cut(s) 21, 226, 324, 341, 383, 437, 564, 617
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 3 cut(s) 31, 76, 535
Mox20I TGGCCA 1 cut(s) 597
MscI TGGCCA 1 cut(s) 597
MseI TTAA 2 cut(s) 225, 554
Msp20I TGGCCA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 10, 241
MunI CAATTG 1 cut(s) 617
MvaI CCWGG 2 cut(s) 10, 241
MvnI CGCG 1 cut(s) 153
NdeII GATC 4 cut(s) 67, 184, 573, 633
NlaIII CATG 3 cut(s) 195, 253, 284
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 602
NspI RCATGY 1 cut(s) 253
PfeI GAWTC 2 cut(s) 14, 445
Pfl23II CGTACG 1 cut(s) 389
PkrI GCNGC 2 cut(s) 248, 494
PleI GAGTC 1 cut(s) 536
PpsI GAGTC 1 cut(s) 536
Psp6I CCWGG 2 cut(s) 8, 239
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 2 cut(s) 8, 239
PspLI CGTACG 1 cut(s) 389
PspN4I GGNNCC 1 cut(s) 219
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 4 cut(s) 126, 380, 391, 454
RsaNI GTAC 4 cut(s) 125, 379, 390, 453
SaqAI TTAA 2 cut(s) 225, 554
SatI GCNGC 2 cut(s) 247, 493
Sau3AI GATC 4 cut(s) 67, 184, 573, 633
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 2 cut(s) 10, 241
SduI GDGCHC 1 cut(s) 319
SetI ASST 8 cut(s) 11, 87, 306, 395, 470, 505, 642, 658
SfaNI GCATC 2 cut(s) 97, 245
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 8 cut(s) 21, 226, 324, 341, 383, 437, 564, 617
SsiI CCGC 2 cut(s) 153, 492
SspMI CTAG 3 cut(s) 480, 662, 689
StyD4I CCNGG 2 cut(s) 8, 239
TaaI ACNGT 2 cut(s) 359, 602
TaiI ACGT 1 cut(s) 395
TaqI TCGA 1 cut(s) 17
TasI AATT 8 cut(s) 21, 226, 324, 341, 383, 437, 564, 617
TatI WGTACW 3 cut(s) 124, 378, 452
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 2 cut(s) 14, 445
Tru1I TTAA 2 cut(s) 225, 554
Tru9I TTAA 2 cut(s) 225, 554
TseFI GTSAC 1 cut(s) 602
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 1 cut(s) 602
TspDTI ATGAA 5 cut(s) 180, 186, 269, 451, 503
VneI GTGCAC 1 cut(s) 315
XagI CCTNNNNNAGG 1 cut(s) 478
XapI RAATTY 2 cut(s) 21, 383
XceI RCATGY 1 cut(s) 253
XspI CTAG 3 cut(s) 480, 662, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.