pycom05g16640

alcohol dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
19923002 .. 19926130
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGTATTACTGCTTGTTATTGGAGGCCATTTTTTCTCAACATCCTCCCAAAAATAGGCAAAGAATTCCTTCTTTTTTCTTTCTAAGTAGAACACCCTGTGAATTACAAACCATGCACAAAATGCATAAGAGTTTTAGAACATTGGTGCACAATGACCATTTCTTGAAGCTCCTAAATAACCAAAAATCTAGGTTCCTCAGACCGGAACTTTACAGCACTTCGGCTCATGCTGGCCGTGCGATCACCAGCAAAGCGGCTGTTGCTTGGGAAGCAGGGAAGCCCTTGGTGATAGAGAAGGTGGAGGTTGCTCCACCGCAAGCCAATGAAGTTAGGGTCCAAATCAAATACACCTCTCTTTGTCACACTGATATCTACTTCTGGGAAGCTAAGGGCCAGACACCATTGTTCCCTAGGATTTTCGGACACGAAGCAGCTGGGCAAATGCTTATTTTTCGTGACAGCGTCGTTGAGAGTGTTGGTGAAGGCGTTAAGAACCTCAAGCCGGGGGATCATGTGCTTCCAGTTTTCACTGGGGAATGTGGGGATTGTCCACATTGCAAGTCTGAGGAAAGCAACATGTGTGAGCTGCTGAGAATCAATTGCGATAGAGGAGTCATGATTGGTGACGGGAAAGCAAGGTTTTCGCAGAATGGGACTCCGATTAATCACTTTCTCGGCACATCCACGTTTAGCGAGTACACAGTTATCCACGAAGGCTGCCTCGCCAAGATCGACCCAAAGGCACCGTTGGATAAAGTCTGCATCCTCAGTTGTGGTGTGTCGACCGGCTTAGGTGCCACTCTGAAAGTAGCAAAACCAAAGAAGGGTTCTTCAGTTGCTATCTTTGGACTTGGAGCTGTTGGCCTCGCTGCTGCAGAAGGTGCAAGAATTTCTGGGGCATCAAGGATTATTGGGGTAGACCTGAATCCCAAAAGATTTGAGCAAGGTATAGTTGAAATTTTACACATTGTCATCGTTAAATTACCATGGTTTAAAAACTATTGGCAAAACATAAGAAACCAATTCTTGCTGAAAACTTTGGTTTACCTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00071 ko00350 ko00592 ko01100 ko01110 ko01120 map00010 map00071 map00350 map00592 map01100 map01110 map01120
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

352

Amino Acids

39.02

Weight (kDa)

9.18

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 107 - 244 5.7e-18 Alcohol dehydrogenase GroES-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 742, 794
AccI GTMKAC 2 cut(s) 782, 918
AciI CCGC 2 cut(s) 254, 314
AclWI GGATC 1 cut(s) 516
AcoI YGGCCR 1 cut(s) 232
AcsI RAATTY 3 cut(s) 63, 888, 957
AcuI CTGAAG 1 cut(s) 816
AdeI CACNNNGTG 1 cut(s) 98
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 4 cut(s) 54, 202, 502, 824
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 2 cut(s) 166, 956
AjnI CCWGG 1 cut(s) 1047
AjuI GAANNNNNNNTTGG 2 cut(s) 811, 843
AluBI AGCT 5 cut(s) 169, 386, 434, 586, 857
AluI AGCT 5 cut(s) 169, 386, 434, 586, 857
Alw21I GWGCWC 1 cut(s) 150
Alw44I GTGCAC 1 cut(s) 146
AlwI GGATC 1 cut(s) 516
AoxI GGCC 4 cut(s) 24, 232, 391, 862
ApaLI GTGCAC 1 cut(s) 146
ApeKI GCWGC 5 cut(s) 431, 586, 717, 869, 872
ApoI RAATTY 3 cut(s) 63, 888, 957
AseI ATTAAT 1 cut(s) 663
Asp700I GAANNNNTTC 1 cut(s) 67
AspA2I CCTAGG 1 cut(s) 410
AspS9I GGNCC 2 cut(s) 334, 391
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 4 cut(s) 235, 298, 491, 635
AvaII GGWCC 1 cut(s) 334
AvrII CCTAGG 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 150
BanI GGYRCC 2 cut(s) 742, 794
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 5 cut(s) 443, 573, 704, 856, 859
BceAI ACGGC 1 cut(s) 219
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciT130I CCWGG 1 cut(s) 1049
BcnI CCSGG 1 cut(s) 504
BfaI CTAG 2 cut(s) 189, 411
BfmI CTRYAG 1 cut(s) 873
BisI GCNGC 6 cut(s) 255, 432, 587, 718, 870, 873
BlnI CCTAGG 1 cut(s) 410
BlsI GCNGC 6 cut(s) 256, 433, 588, 719, 871, 874
Bme1390I CCNGG 2 cut(s) 504, 1049
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 334, 391
BmiI GGNNCC 4 cut(s) 194, 335, 744, 796
BmrFI CCNGG 2 cut(s) 504, 1049
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 771, 908
BmuI ACTGGG 1 cut(s) 540
Bpu10I CCTNAGC 2 cut(s) 387, 790
BpuEI CTTGAG 1 cut(s) 482
BpuMI CCSGG 1 cut(s) 504
BsaJI CCNNGG 4 cut(s) 282, 410, 503, 986
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 4 cut(s) 54, 202, 502, 824
Bse118I RCCGGY 1 cut(s) 785
Bse1I ACTGG 2 cut(s) 521, 535
Bse3DI GCAATG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 1049
BseDI CCNNGG 4 cut(s) 282, 410, 503, 986
BseGI GGATG 3 cut(s) 40, 680, 762
BseLI CCNNNNNNNGG 4 cut(s) 54, 202, 502, 824
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 4 cut(s) 211, 555, 581, 781
BseNI ACTGG 2 cut(s) 521, 535
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 5 cut(s) 443, 573, 704, 856, 859
BseYI CCCAGC 1 cut(s) 434
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 4 cut(s) 26, 234, 393, 864
BshNI GGYRCC 2 cut(s) 742, 794
BsiEI CGRYCG 1 cut(s) 786
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 3 cut(s) 203, 503, 786
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 4 cut(s) 54, 202, 502, 824
BsmFI GGGAC 1 cut(s) 667
BsnI GGCC 4 cut(s) 26, 234, 393, 864
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 240, 508, 729
Bsp19I CCATGG 1 cut(s) 986
BspACI CCGC 2 cut(s) 254, 314
BspANI GGCC 4 cut(s) 26, 234, 393, 864
BspCNI CTCAG 4 cut(s) 210, 556, 582, 780
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 4 cut(s) 194, 335, 744, 796
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 1 cut(s) 516
BspT107I GGYRCC 2 cut(s) 742, 794
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 785
BsrI ACTGG 2 cut(s) 521, 535
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 4 cut(s) 282, 410, 503, 986
BssMI GATC 3 cut(s) 240, 508, 729
BssT1I CCWWGG 3 cut(s) 282, 410, 986
Bst2UI CCWGG 1 cut(s) 1049
Bst4CI ACNGT 2 cut(s) 703, 747
BstAPI GCANNNNNTGC 2 cut(s) 121, 881
BstC8I GCNNGC 2 cut(s) 232, 318
BstDEI CTNAG 7 cut(s) 83, 197, 387, 564, 590, 767, 790
BstDSI CCRYGG 1 cut(s) 986
BstF5I GGATG 3 cut(s) 40, 680, 762
BstKTI GATC 3 cut(s) 243, 511, 732
BstMBI GATC 3 cut(s) 240, 508, 729
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 5 cut(s) 121, 236, 260, 269, 881
BstNI CCWGG 1 cut(s) 1049
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 2 cut(s) 502, 1047
BstSFI CTRYAG 1 cut(s) 873
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 5 cut(s) 443, 573, 704, 856, 859
BsuRI GGCC 4 cut(s) 26, 234, 393, 864
BtgI CCRYGG 1 cut(s) 986
BtsCI GGATG 3 cut(s) 40, 680, 762
BtsIMutI CAGTG 2 cut(s) 363, 528
Cac8I GCNNGC 2 cut(s) 232, 318
CciI TCATGA 1 cut(s) 615
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 2 cut(s) 334, 391
CseI GACGC 1 cut(s) 451
CsiI ACCWGGT 1 cut(s) 1047
Csp6I GTAC 1 cut(s) 697
CviAII CATG 6 cut(s) 112, 227, 512, 577, 616, 987
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 7 cut(s) 83, 197, 387, 564, 590, 767, 790
DpnI GATC 3 cut(s) 242, 510, 731
DpnII GATC 3 cut(s) 240, 508, 729
DraI TTTAAA 1 cut(s) 994
DraIII CACNNNGTG 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 282, 410, 986
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 816
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 1047
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 3 cut(s) 282, 410, 986
EcoT22I ATGCAT 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 282, 410, 986
FaeI CATG 6 cut(s) 115, 230, 515, 580, 619, 990
FaiI YATR 9 cut(s) 113, 126, 228, 513, 578, 617, 950, 988, 1013
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 1 cut(s) 667
FatI CATG 6 cut(s) 111, 226, 511, 576, 615, 986
FblI GTMKAC 2 cut(s) 782, 918
Fnu4HI GCNGC 6 cut(s) 255, 432, 587, 718, 870, 873
FokI GGATG 3 cut(s) 27, 667, 749
Fsp4HI GCNGC 6 cut(s) 255, 432, 587, 718, 870, 873
FspBI CTAG 2 cut(s) 189, 411
GluI GCNGC 6 cut(s) 255, 432, 587, 718, 870, 873
GsaI CCCAGC 1 cut(s) 438
HaeIII GGCC 4 cut(s) 26, 234, 393, 864
HapII CCGG 3 cut(s) 203, 503, 786
HgaI GACGC 1 cut(s) 451
Hin1II CATG 6 cut(s) 115, 230, 515, 580, 619, 990
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HinfI GANTC 4 cut(s) 594, 612, 655, 925
HpaII CCGG 3 cut(s) 203, 503, 786
HphI GGTGA 4 cut(s) 235, 298, 491, 635
Hpy166II GTNNAC 6 cut(s) 148, 551, 699, 783, 919, 1045
Hpy188I TCNGA 5 cut(s) 200, 422, 565, 660, 804
Hpy188III TCNNGA 3 cut(s) 163, 455, 616
Hpy8I GTNNAC 6 cut(s) 148, 551, 699, 783, 919, 1045
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 6 cut(s) 78, 289, 476, 707, 817, 872
HpyCH4III ACNGT 2 cut(s) 703, 747
HpyCH4IV ACGT 1 cut(s) 686
HpyCH4V TGCA 7 cut(s) 115, 124, 148, 558, 762, 875, 884
HpyF10VI GCNNNNNNNGC 5 cut(s) 121, 236, 260, 269, 881
HpyF3I CTNAG 7 cut(s) 83, 197, 387, 564, 590, 767, 790
HpySE526I ACGT 1 cut(s) 686
Hsp92II CATG 6 cut(s) 115, 230, 515, 580, 619, 990
Kzo9I GATC 3 cut(s) 240, 508, 729
LmnI GCTCC 3 cut(s) 174, 313, 854
Lsp1109I GCAGC 5 cut(s) 443, 573, 704, 856, 859
LweI GCATC 2 cut(s) 771, 908
MabI ACCWGGT 1 cut(s) 1047
MaeI CTAG 2 cut(s) 189, 411
MaeII ACGT 1 cut(s) 686
MaeIII GTNAC 3 cut(s) 359, 455, 623
MalI GATC 3 cut(s) 242, 510, 731
MboI GATC 3 cut(s) 240, 508, 729
MboII GAAGA 1 cut(s) 822
MfeI CAATTG 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 7 cut(s) 63, 101, 598, 888, 957, 980, 1022
MlyI GAGTC 2 cut(s) 621, 649
MmeI TCCRAC 1 cut(s) 729
Mph1103I ATGCAT 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 67
MseI TTAA 4 cut(s) 489, 663, 978, 993
MspA1I CMGCKG 1 cut(s) 434
MspI CCGG 3 cut(s) 203, 503, 786
MspR9I CCNGG 2 cut(s) 504, 1049
MunI CAATTG 1 cut(s) 598
MvaI CCWGG 1 cut(s) 1049
MwoI GCNNNNNNNGC 5 cut(s) 121, 236, 260, 269, 881
NciI CCSGG 1 cut(s) 504
NcoI CCATGG 1 cut(s) 986
NdeII GATC 3 cut(s) 240, 508, 729
NlaIII CATG 6 cut(s) 115, 230, 515, 580, 619, 990
NlaIV GGNNCC 4 cut(s) 194, 335, 744, 796
NmeAIII GCCGAG 1 cut(s) 654
NmuCI GTSAC 3 cut(s) 359, 455, 623
NsiI ATGCAT 1 cut(s) 126
NspI RCATGY 1 cut(s) 580
PagI TCATGA 1 cut(s) 615
PciI ACATGT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 594, 925
PflFI GACNNNGTC 1 cut(s) 461
PkrI GCNGC 6 cut(s) 256, 433, 588, 719, 871, 874
PleI GAGTC 2 cut(s) 620, 649
PpsI GAGTC 2 cut(s) 620, 649
PscI ACATGT 1 cut(s) 576
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 1047
PspFI CCCAGC 1 cut(s) 434
PspGI CCWGG 1 cut(s) 1047
PspN4I GGNNCC 4 cut(s) 194, 335, 744, 796
PspPI GGNCC 2 cut(s) 334, 391
PstI CTGCAG 1 cut(s) 877
PsyI GACNNNGTC 1 cut(s) 461
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SalI GTCGAC 1 cut(s) 781
SaqAI TTAA 4 cut(s) 489, 663, 978, 993
SatI GCNGC 6 cut(s) 255, 432, 587, 718, 870, 873
Sau3AI GATC 3 cut(s) 240, 508, 729
Sau96I GGNCC 2 cut(s) 334, 391
SchI GAGTC 2 cut(s) 621, 649
ScrFI CCNGG 2 cut(s) 504, 1049
SduI GDGCHC 1 cut(s) 150
SexAI ACCWGGT 1 cut(s) 1047
SfaNI GCATC 2 cut(s) 771, 908
SfcI CTRYAG 1 cut(s) 873
SinI GGWCC 1 cut(s) 334
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 7 cut(s) 63, 101, 598, 888, 957, 980, 1022
SsiI CCGC 2 cut(s) 254, 314
SspMI CTAG 2 cut(s) 189, 411
StyD4I CCNGG 2 cut(s) 502, 1047
StyI CCWWGG 3 cut(s) 282, 410, 986
TaaI ACNGT 2 cut(s) 703, 747
TaiI ACGT 1 cut(s) 689
TaqI TCGA 2 cut(s) 732, 782
TasI AATT 7 cut(s) 63, 101, 598, 888, 957, 980, 1022
TatI WGTACW 1 cut(s) 696
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 594, 925
Tru1I TTAA 4 cut(s) 489, 663, 978, 993
Tru9I TTAA 4 cut(s) 489, 663, 978, 993
TscAI CASTG 2 cut(s) 370, 535
TseFI GTSAC 3 cut(s) 359, 455, 623
TseI GCWGC 5 cut(s) 431, 586, 717, 869, 872
Tsp45I GTSAC 3 cut(s) 359, 455, 623
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 2 cut(s) 370, 535
Tth111I GACNNNGTC 1 cut(s) 461
VneI GTGCAC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 334
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 3 cut(s) 63, 888, 957
XceI RCATGY 1 cut(s) 580
XmaJI CCTAGG 1 cut(s) 410
XmiI GTMKAC 2 cut(s) 782, 918
XmnI GAANNNNTTC 1 cut(s) 67
XspI CTAG 2 cut(s) 189, 411
Zsp2I ATGCAT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.