pycom05g18680

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
21557617 .. 21558563
947 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 696 bp
ATGGAAGACGAGTCCTCGTATTACGACAGAACCAAGGAAGTCGAGTTCAATGAAACTAAAGCCGGAGTTAATGGCCTTGTGGACTCCGGGATCACAAAAGTGCCCAAGTTCCTCATCCATCCACCACATAGCCTTTCAAATTCAGAAACAACAGATGTAGACTTTAAGGTTCCTGTCATAGACTTCGACGGCTTTGATCAAGATTTTCGGCGTGCGCAGACTGTGAAGGAGATTCGTGAAGCATCTGAAACGTGGGGGTTTCATGAGCAGCCGAAGGAGCTGAAGATGGAGTGGTATTCTTGTGACTTGAAACGTAGAGTGAGATACTACTGCAATGGAGATCTGTTGGTGCAGAAAGCAGCGAATTGGAGAGACTCGATATTGTTTGATTTCCAGAACGGTCCATTGGACCCCGAGACATTTCCCTTCGTTTGCAGGTACTTCGCTAAACAAACTGTTCAAAAGTGCATGAAGACTGAACATCTGGTGTGCCATTACTACCCGAGCTGTCCAGAACCGGAGTTGACCCCCGGCACTACCAAGCATTCAGATCCATCCTCTTTAACTGTACTCTTGCAAGACAATCTCGGCGGCCTGCAAGTCCTCCATCAAGGTCACTGGGTTGATGTTCCTCCAACTCATGGAGCTCTTGTGGCCAACGTGGGTGATCTTATGCAAGTAAGCTCATCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

232

Amino Acids

26.33

Weight (kDa)

5.23

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 160 - 229 2.8e-21 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 216
Acc36I ACCTGC 1 cut(s) 426
AccB7I CCANNNNNTGG 1 cut(s) 641
AccI GTMKAC 1 cut(s) 159
AciI CCGC 1 cut(s) 591
AclWI GGATC 2 cut(s) 98, 545
AcoI YGGCCR 1 cut(s) 654
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 302
AfaI GTAC 2 cut(s) 440, 570
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 4 cut(s) 49, 138, 310, 461
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AleI CACNNNNGTG 1 cut(s) 98
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 4 cut(s) 280, 507, 647, 684
AluI AGCT 4 cut(s) 280, 507, 647, 684
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 366, 410
AlwI GGATC 2 cut(s) 98, 545
Ama87I CYCGRG 2 cut(s) 413, 502
AoxI GGCC 3 cut(s) 73, 592, 654
ApeKI GCWGC 2 cut(s) 268, 359
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 2 cut(s) 401, 409
AsuC2I CCSGG 2 cut(s) 88, 531
AsuHPI GGTGA 1 cut(s) 677
AvaI CYCGRG 2 cut(s) 413, 502
AvaII GGWCC 2 cut(s) 401, 409
BaeGI GKGCMC 1 cut(s) 105
BalI TGGCCA 1 cut(s) 656
BanII GRGCYC 1 cut(s) 649
BbsI GAAGAC 2 cut(s) 12, 479
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 2 cut(s) 280, 371
BccI CCATC 4 cut(s) 126, 280, 562, 615
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BclI TGATCA 1 cut(s) 196
BcnI CCSGG 2 cut(s) 88, 531
BcoDI GTCTC 2 cut(s) 366, 410
BfuAI ACCTGC 1 cut(s) 426
BglII AGATCT 1 cut(s) 340
BisI GCNGC 3 cut(s) 269, 360, 592
BlsI GCNGC 3 cut(s) 270, 361, 593
Bme1390I CCNGG 2 cut(s) 88, 531
Bme18I GGWCC 2 cut(s) 401, 409
BmeT110I CYCGRG 2 cut(s) 413, 502
BmgT120I GGNCC 2 cut(s) 401, 409
BmiI GGNNCC 2 cut(s) 171, 411
BmrFI CCNGG 2 cut(s) 88, 531
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 251
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 2 cut(s) 12, 479
BpuMI CCSGG 2 cut(s) 88, 531
BsaJI CCNNGG 2 cut(s) 33, 529
BsaWI WCCGGW 1 cut(s) 517
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 340
BseDI CCNNGG 2 cut(s) 33, 529
BseGI GGATG 4 cut(s) 114, 118, 554, 689
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 1 cut(s) 340
BseNI ACTGG 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 2 cut(s) 280, 371
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 3 cut(s) 75, 594, 656
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 2 cut(s) 413, 502
BsiSI CCGG 4 cut(s) 63, 87, 518, 531
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 2 cut(s) 366, 410
BsmI GAATGC 1 cut(s) 544
BsnI GGCC 3 cut(s) 75, 594, 656
BsoBI CYCGRG 2 cut(s) 413, 502
Bsp1286I GDGCHC 2 cut(s) 105, 649
Bsp143I GATC 5 cut(s) 90, 196, 340, 550, 667
BspACI CCGC 1 cut(s) 591
BspANI GGCC 3 cut(s) 75, 594, 656
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 171, 411
BspMI ACCTGC 1 cut(s) 426
BspPI GGATC 2 cut(s) 98, 545
BsrDI GCAATG 1 cut(s) 340
BsrI ACTGG 1 cut(s) 623
BssECI CCNNGG 2 cut(s) 33, 529
BssMI GATC 5 cut(s) 90, 196, 340, 550, 667
BssT1I CCWWGG 1 cut(s) 33
Bst4CI ACNGT 4 cut(s) 223, 401, 457, 568
BstC8I GCNNGC 2 cut(s) 213, 596
BstF5I GGATG 4 cut(s) 114, 118, 554, 689
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 5 cut(s) 93, 199, 343, 553, 670
BstMAI GTCTC 2 cut(s) 366, 410
BstMBI GATC 5 cut(s) 90, 196, 340, 550, 667
BstMWI GCNNNNNNNGC 2 cut(s) 277, 653
BstSCI CCNGG 2 cut(s) 86, 529
BstSLI GKGCMC 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 280, 371
BstV2I GAAGAC 2 cut(s) 12, 479
BstX2I RGATCY 2 cut(s) 340, 550
BstYI RGATCY 2 cut(s) 340, 550
BsuRI GGCC 3 cut(s) 75, 594, 656
BtsCI GGATG 4 cut(s) 114, 118, 554, 689
BtsIMutI CAGTG 1 cut(s) 616
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 213, 596
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 2 cut(s) 401, 409
Csp6I GTAC 2 cut(s) 439, 569
CviAII CATG 3 cut(s) 263, 469, 641
CviQI GTAC 2 cut(s) 439, 569
DpnI GATC 5 cut(s) 92, 198, 342, 552, 669
DpnII GATC 5 cut(s) 90, 196, 340, 550, 667
EaeI YGGCCR 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 401, 409
Eco53kI GAGCTC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 2 cut(s) 413, 502
EcoICRI GAGCTC 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 33
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 3 cut(s) 266, 472, 644
FaiI YATR 6 cut(s) 129, 179, 264, 470, 642, 674
FatI CATG 3 cut(s) 262, 468, 640
FbaI TGATCA 1 cut(s) 196
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 269, 360, 592
FokI GGATG 4 cut(s) 101, 105, 541, 676
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 3 cut(s) 269, 360, 592
FspI TGCGCA 1 cut(s) 216
GlaI GCGC 1 cut(s) 216
GluI GCNGC 3 cut(s) 269, 360, 592
HaeIII GGCC 3 cut(s) 75, 594, 656
HapII CCGG 4 cut(s) 63, 87, 518, 531
HhaI GCGC 1 cut(s) 217
Hin1II CATG 3 cut(s) 266, 472, 644
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HinfI GANTC 4 cut(s) 11, 83, 232, 374
HpaII CCGG 4 cut(s) 63, 87, 518, 531
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 3 cut(s) 82, 160, 525
Hpy188I TCNGA 3 cut(s) 145, 247, 550
Hpy188III TCNNGA 5 cut(s) 200, 236, 263, 394, 512
Hpy8I GTNNAC 3 cut(s) 82, 160, 525
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 220, 268, 436
HpyCH4III ACNGT 4 cut(s) 223, 401, 457, 568
HpyCH4IV ACGT 3 cut(s) 251, 313, 660
HpyCH4V TGCA 7 cut(s) 333, 352, 435, 468, 577, 598, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 653
HpySE526I ACGT 3 cut(s) 251, 313, 660
Hsp92II CATG 3 cut(s) 266, 472, 644
HspAI GCGC 1 cut(s) 215
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 5 cut(s) 90, 196, 340, 550, 667
LmnI GCTCC 2 cut(s) 277, 644
Lsp1109I GCAGC 2 cut(s) 280, 371
LweI GCATC 1 cut(s) 251
MaeII ACGT 3 cut(s) 251, 313, 660
MaeIII GTNAC 2 cut(s) 302, 614
MalI GATC 5 cut(s) 92, 198, 342, 552, 669
MboI GATC 5 cut(s) 90, 196, 340, 550, 667
MboII GAAGA 3 cut(s) 17, 295, 484
MflI RGATCY 2 cut(s) 340, 550
MhlI GDGCHC 2 cut(s) 105, 649
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 2 cut(s) 139, 364
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 3 cut(s) 20, 77, 368
MmeI TCCRAC 1 cut(s) 659
MnlI CCTC 5 cut(s) 25, 122, 568, 614, 642
Mox20I TGGCCA 1 cut(s) 656
MscI TGGCCA 1 cut(s) 656
MseI TTAA 3 cut(s) 69, 165, 563
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 656
MspI CCGG 4 cut(s) 63, 87, 518, 531
MspR9I CCNGG 2 cut(s) 88, 531
Mva1269I GAATGC 1 cut(s) 544
MwoI GCNNNNNNNGC 2 cut(s) 277, 653
NciI CCSGG 2 cut(s) 88, 531
NdeII GATC 5 cut(s) 90, 196, 340, 550, 667
NlaIII CATG 3 cut(s) 266, 472, 644
NlaIV GGNNCC 2 cut(s) 171, 411
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 2 cut(s) 302, 614
NsbI TGCGCA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 98
PagI TCATGA 1 cut(s) 262
PctI GAATGC 1 cut(s) 544
PfeI GAWTC 1 cut(s) 232
PflMI CCANNNNNTGG 1 cut(s) 641
PfoI TCCNGGA 1 cut(s) 86
PkrI GCNGC 3 cut(s) 270, 361, 593
PleI GAGTC 3 cut(s) 19, 77, 368
PpsI GAGTC 3 cut(s) 19, 77, 368
Psp124BI GAGCTC 1 cut(s) 649
PspN4I GGNNCC 2 cut(s) 171, 411
PspPI GGNCC 2 cut(s) 401, 409
PsuI RGATCY 2 cut(s) 340, 550
RsaI GTAC 2 cut(s) 440, 570
RsaNI GTAC 2 cut(s) 439, 569
RseI CAYNNNNRTG 1 cut(s) 98
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 3 cut(s) 69, 165, 563
SatI GCNGC 3 cut(s) 269, 360, 592
Sau3AI GATC 5 cut(s) 90, 196, 340, 550, 667
Sau96I GGNCC 2 cut(s) 401, 409
SchI GAGTC 3 cut(s) 20, 77, 368
ScrFI CCNGG 2 cut(s) 88, 531
SduI GDGCHC 2 cut(s) 105, 649
SfaNI GCATC 1 cut(s) 251
SinI GGWCC 2 cut(s) 401, 409
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 2 cut(s) 139, 364
SsiI CCGC 1 cut(s) 591
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 2 cut(s) 86, 529
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 4 cut(s) 223, 401, 457, 568
TaiI ACGT 3 cut(s) 254, 316, 663
TaqI TCGA 3 cut(s) 42, 186, 377
TasI AATT 2 cut(s) 139, 364
TatI WGTACW 1 cut(s) 568
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 3 cut(s) 69, 165, 563
Tru9I TTAA 3 cut(s) 69, 165, 563
TscAI CASTG 1 cut(s) 623
TseFI GTSAC 2 cut(s) 302, 614
TseI GCWGC 2 cut(s) 268, 359
Tsp45I GTSAC 2 cut(s) 302, 614
TspDTI ATGAA 3 cut(s) 66, 251, 485
TspRI CASTG 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 401, 409
XapI RAATTY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.