pycom05g20290

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
23101519 .. 23102067
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
ATGATCCTTTGTCGATTGGCTGTTACTATTGTGTACTATTTTGCATATCAAAGAAAAAGCATCCTACTAGATTGGCCTAAGCGTTTTGACATTATTAATGGGATTGCTCGGGGGCTCCGCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATCGAGATCTCAAAGCCGGCAACATTCTATTAGACAGTGAATTTAACCCCAAAATTTTAGACTTTGGCCTGGCAAGAAGTTTTGGAGGAAATGAAACTACAGCAACGACAAATAAAGTGGTTGGAACATATGGTTACATGTCCCCAGAATATGCGATCGATGGGCTCTACTCGATAAAATCTGACATGTATGGCTTTGGTGTTATGGTGCTTGAGATAGTGAAGGGAAAAGAAACAGAGGATTTTCTCATCCAGACCACAGCCTTAACCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

143

Amino Acids

16.14

Weight (kDa)

8.52

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 23 - 128 1.8e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 27 - 129 6.4e-26 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 118
AcsI RAATTY 2 cut(s) 194, 207
AfaI GTAC 1 cut(s) 35
AflIII ACRYGT 2 cut(s) 291, 339
AjnI CCWGG 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 2 cut(s) 74, 220
ApoI RAATTY 2 cut(s) 194, 207
AseI ATTAAT 1 cut(s) 96
AvaI CYCGRG 1 cut(s) 108
BanII GRGCYC 2 cut(s) 117, 321
BccI CCATC 1 cut(s) 308
BciT130I CCWGG 1 cut(s) 224
BfaI CTAG 3 cut(s) 68, 138, 427
BfmI CTRYAG 1 cut(s) 252
BglII AGATCT 1 cut(s) 160
Bme1390I CCNGG 1 cut(s) 224
BmeT110I CYCGRG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 69
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 312
Bse118I RCCGGY 1 cut(s) 170
BseBI CCWGG 1 cut(s) 224
BseCI ATCGAT 1 cut(s) 312
BseGI GGATG 2 cut(s) 60, 402
BseMII CTCAG 1 cut(s) 134
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 76, 222
BshVI ATCGAT 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 2 cut(s) 76, 222
BsoBI CYCGRG 1 cut(s) 108
Bsp1286I GDGCHC 2 cut(s) 117, 321
Bsp143I GATC 3 cut(s) 3, 160, 309
BspACI CCGC 1 cut(s) 118
BspANI GGCC 2 cut(s) 76, 222
BspCNI CTCAG 1 cut(s) 135
BspDI ATCGAT 1 cut(s) 312
BspLI GGNNCC 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssMI GATC 3 cut(s) 3, 160, 309
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 2 cut(s) 78, 143
BstF5I GGATG 2 cut(s) 60, 402
BstKTI GATC 3 cut(s) 6, 163, 312
BstMBI GATC 3 cut(s) 3, 160, 309
BstMCI CGRYCG 1 cut(s) 312
BstNI CCWGG 1 cut(s) 224
BstNSI RCATGY 2 cut(s) 295, 343
BstSCI CCNGG 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 160
BstYI RGATCY 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 2 cut(s) 76, 222
BsuTUI ATCGAT 1 cut(s) 312
BtsCI GGATG 2 cut(s) 60, 402
BtsIMutI CAGTG 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 170
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 34
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 2 cut(s) 292, 340
CviJI RGCY 8 cut(s) 20, 76, 115, 170, 222, 319, 348, 416
CviKI_1 RGCY 8 cut(s) 20, 76, 115, 170, 222, 319, 348, 416
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 78, 143
DpnI GATC 3 cut(s) 5, 162, 311
DpnII GATC 3 cut(s) 3, 160, 309
Eco24I GRGCYC 2 cut(s) 117, 321
Eco88I CYCGRG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 222
EcoT38I GRGCYC 2 cut(s) 117, 321
FaeI CATG 2 cut(s) 295, 343
FaiI YATR 8 cut(s) 46, 283, 285, 293, 306, 341, 345, 359
FaqI GGGAC 1 cut(s) 280
FatI CATG 2 cut(s) 291, 339
FauNDI CATATG 1 cut(s) 283
FokI GGATG 2 cut(s) 47, 389
FriOI GRGCYC 2 cut(s) 117, 321
FspBI CTAG 3 cut(s) 68, 138, 427
HaeIII GGCC 2 cut(s) 76, 222
HapII CCGG 1 cut(s) 171
Hin1II CATG 2 cut(s) 295, 343
HinfI GANTC 1 cut(s) 134
HpaII CCGG 1 cut(s) 171
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 4 cut(s) 131, 138, 158, 406
Hpy8I GTNNAC 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 191
HpyCH4V TGCA 1 cut(s) 44
HpyF3I CTNAG 2 cut(s) 78, 143
Hsp92II CATG 2 cut(s) 295, 343
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 3, 160, 309
LmnI GCTCC 1 cut(s) 120
LpnPI CCDG 5 cut(s) 184, 209, 236, 312, 419
LweI GCATC 1 cut(s) 69
MaeI CTAG 3 cut(s) 68, 138, 427
MaeIII GTNAC 2 cut(s) 22, 287
MalI GATC 3 cut(s) 5, 162, 311
MboI GATC 3 cut(s) 3, 160, 309
MboII GAAGA 1 cut(s) 116
MflI RGATCY 1 cut(s) 160
MhlI GDGCHC 2 cut(s) 117, 321
MluCI AATT 3 cut(s) 150, 194, 207
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 2 cut(s) 233, 385
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 3 cut(s) 96, 198, 419
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
NaeI GCCGGC 1 cut(s) 172
NdeI CATATG 1 cut(s) 283
NdeII GATC 3 cut(s) 3, 160, 309
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 295, 343
NlaIV GGNNCC 1 cut(s) 116
NspI RCATGY 2 cut(s) 295, 343
PciI ACATGT 2 cut(s) 291, 339
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 134
Ple19I CGATCG 1 cut(s) 312
PscI ACATGT 2 cut(s) 291, 339
PshBI ATTAAT 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 116
PsuI RGATCY 1 cut(s) 160
PvuI CGATCG 1 cut(s) 312
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
SaqAI TTAA 3 cut(s) 96, 198, 419
Sau3AI GATC 3 cut(s) 3, 160, 309
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 2 cut(s) 117, 321
SetI ASST 1 cut(s) 425
SfaNI GCATC 1 cut(s) 69
SfcI CTRYAG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 3 cut(s) 150, 194, 207
SsiI CCGC 1 cut(s) 118
SspMI CTAG 3 cut(s) 68, 138, 427
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 191
TaqI TCGA 4 cut(s) 13, 157, 312, 326
TasI AATT 3 cut(s) 150, 194, 207
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 3 cut(s) 96, 198, 419
Tru9I TTAA 3 cut(s) 96, 198, 419
TscAI CASTG 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 116, 143, 261
TspRI CASTG 1 cut(s) 196
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 2 cut(s) 194, 207
XbaI TCTAGA 1 cut(s) 137
XceI RCATGY 2 cut(s) 295, 343
XspI CTAG 3 cut(s) 68, 138, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.