pycom05g20360

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
23132630 .. 23134542
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGACCCAATTATTGAATGAAAGAAGCAGTTATGCAGCTTCTTCTTCTCTCTTCGATTCCTCTGGTTTCTTTGACTTCTCTCTCGTTTCGGTAGATTACGTCTCTGTTGACATATCACACGTTTATACCACATTTCAGATCCCACCAAGGCAAAGAAAAATCCCAATTAAGATATGCCTGCATCTTTTGCTGCTCTTCCTCCTCGTAACTACCGCTGCAACATTTTCCGCAGCACAAGTCAACGATGCAACCACATTTCAGTCAATTGCAGATGGTGAGACCATAATCTCAGATGGTGGCACCTTTGAGCTTGGTTTTTTCAGTCCCGGTGCTTCAAATAAACGCTACGTGGGGATATGGTACAAGAAGATACCGGATATGACCGTTGTTTGGGTAGCAAACCGGGACTTTCCTCTCTCTGCTTCATCCGGTGTTCTGAAGGTCACCAACCCTGGGATTCTTGTCCTTGTCGACCAGAACAACACAGTTTGGTCCTCCAACACATCAATACCTACACAGAATCCAGTGGCACGGCTTTTGGATTCGGGAAATCTTGTTGTGATAGATGGTAATGATCACAACGTTGTCCCGGAGAATTTTCTGTGGCAGAGTTTTGATTACCCGGGTGATACGCTCCTTCCTGGTATGCAGCTTGGTAGGAACAAGGTAACAGGTTTTTATTGGCAACTTACATCATGGAAAAGTCCCCAGGATCCTTCTCAAGGAAGAACACCGGCTGATACGTGTGACAGCTATGGAATATGTGGGCCAAATGGTGCATGTAATATTGACAACCCCCCGCTATGTGGTTGCTTGGCAGGATTTAGACCAAATTTTCAACAAGAAGGGCCCCCCCGGCGTGCAACATATGCCTGTATGAGAATGACTCCACTGAATTGCAGTGGAGACGACTTTCTGAAGTACTCTGGGATGAAACTGCCAAGCACAGAAAAATCCTGGCATAACACAAGTATGAACCTCCATGAATGTGAGATGATGTGCAGAAAACTGAGATCGGACCAAAAGGAAGATCTGGAGTTACCATTATTTGACTTGGGTACTGTAACTTGTGCTACCAATGATTTTTCAAACCACAATAAAGTTGGGGAAGGTGGCTTCGGATCTGTCTATAAGGTGAAGTCACAGTCCACGCTACAATTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

389

Amino Acids

42.73

Weight (kDa)

5.54

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 128 - 234 4e-36 D-mannose binding lectin
S_locus_glycop PF00954 242 - 278 2.9e-10 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 471
AciI CCGC 3 cut(s) 213, 228, 800
AclI AACGTT 1 cut(s) 582
AclWI GGATC 4 cut(s) 133, 707, 720, 1129
AcsI RAATTY 2 cut(s) 595, 832
AcuI CTGAAG 2 cut(s) 458, 938
AfaI GTAC 3 cut(s) 362, 923, 1060
AfiI CCNNNNNNNGG 4 cut(s) 390, 453, 722, 806
AflIII ACRYGT 2 cut(s) 118, 743
AgsI TTSAA 4 cut(s) 16, 336, 839, 1089
AjnI CCWGG 4 cut(s) 451, 640, 708, 956
AluBI AGCT 5 cut(s) 38, 310, 652, 753, 1163
AluI AGCT 5 cut(s) 38, 310, 652, 753, 1163
Alw26I GTCTC 3 cut(s) 106, 272, 900
AlwI GGATC 4 cut(s) 133, 707, 720, 1129
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 2 cut(s) 767, 848
ApaI GGGCCC 1 cut(s) 852
ApeKI GCWGC 5 cut(s) 35, 190, 215, 230, 649
ApoI RAATTY 2 cut(s) 595, 832
AspS9I GGNCC 5 cut(s) 492, 767, 848, 849, 1018
AsuC2I CCSGG 6 cut(s) 327, 404, 590, 623, 624, 856
AsuHPI GGTGA 4 cut(s) 287, 436, 638, 1147
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 2 cut(s) 492, 1018
BaeGI GKGCMC 1 cut(s) 852
BamHI GGATCC 1 cut(s) 712
BanI GGYRCC 1 cut(s) 299
BanII GRGCYC 1 cut(s) 852
BbvI GCAGC 5 cut(s) 47, 177, 202, 242, 661
BccI CCATC 3 cut(s) 266, 287, 560
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 4 cut(s) 453, 642, 710, 958
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 6 cut(s) 327, 404, 590, 623, 624, 856
BcoDI GTCTC 3 cut(s) 106, 272, 900
BglI GCCNNNNNGGC 1 cut(s) 856
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 5 cut(s) 36, 191, 216, 231, 650
BlsI GCNGC 5 cut(s) 37, 192, 217, 232, 651
BmcAI AGTACT 1 cut(s) 923
Bme18I GGWCC 2 cut(s) 492, 1018
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 5 cut(s) 492, 767, 848, 849, 1018
BmiI GGNNCC 4 cut(s) 301, 714, 850, 851
BmsI GCATC 2 cut(s) 190, 235
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpmI CTGGAG 1 cut(s) 1055
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 6 cut(s) 327, 404, 590, 623, 624, 856
BsaAI YACGTR 2 cut(s) 349, 744
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 6 cut(s) 146, 451, 452, 622, 708, 854
BsaWI WCCGGW 2 cut(s) 373, 428
Bsc4I CCNNNNNNNGG 4 cut(s) 390, 453, 722, 806
Bse118I RCCGGY 1 cut(s) 733
Bse1I ACTGG 1 cut(s) 524
BseBI CCWGG 4 cut(s) 453, 642, 710, 958
BseDI CCNNGG 6 cut(s) 146, 451, 452, 622, 708, 854
BseGI GGATG 2 cut(s) 425, 936
BseLI CCNNNNNNNGG 4 cut(s) 390, 453, 722, 806
BseMII CTCAG 2 cut(s) 303, 1001
BseNI ACTGG 1 cut(s) 524
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 852
BseXI GCAGC 5 cut(s) 47, 177, 202, 242, 661
BsgI GTGCAG 1 cut(s) 1021
BshFI GGCC 2 cut(s) 769, 850
BshNI GGYRCC 1 cut(s) 299
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 8 cut(s) 327, 374, 403, 429, 590, 623, 734, 856
BslFI GGGAC 4 cut(s) 309, 419, 572, 690
BslI CCNNNNNNNGG 4 cut(s) 390, 453, 722, 806
BsmAI GTCTC 3 cut(s) 106, 272, 900
BsmBI CGTCTC 2 cut(s) 106, 900
BsmFI GGGAC 4 cut(s) 309, 419, 572, 690
BsnI GGCC 2 cut(s) 769, 850
Bso31I GGTCTC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 622
Bsp120I GGGCCC 1 cut(s) 848
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
BspACI CCGC 3 cut(s) 213, 228, 800
BspANI GGCC 2 cut(s) 769, 850
BspCNI CTCAG 2 cut(s) 302, 1002
BspLI GGNNCC 4 cut(s) 301, 714, 850, 851
BspPI GGATC 4 cut(s) 133, 707, 720, 1129
BspQI GCTCTTC 1 cut(s) 200
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 733
BsrI ACTGG 1 cut(s) 524
BssAI RCCGGY 1 cut(s) 733
BssECI CCNNGG 6 cut(s) 146, 451, 452, 622, 708, 854
BssMI GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 4 cut(s) 453, 642, 710, 958
Bst4CI ACNGT 4 cut(s) 385, 487, 1063, 1146
Bst6I CTCTTC 2 cut(s) 56, 200
BstAPI GCANNNNNTGC 2 cut(s) 187, 869
BstBAI YACGTR 2 cut(s) 349, 744
BstC8I GCNNGC 2 cut(s) 179, 861
BstDEI CTNAG 2 cut(s) 289, 1010
BstEII GGTNACC 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 720
BstF5I GGATG 2 cut(s) 425, 936
BstKTI GATC 6 cut(s) 141, 577, 715, 1016, 1033, 1124
BstMAI GTCTC 3 cut(s) 106, 272, 900
BstMBI GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
BstMWI GCNNNNNNNGC 3 cut(s) 187, 856, 869
BstNI CCWGG 4 cut(s) 453, 642, 710, 958
BstNSI RCATGY 1 cut(s) 783
BstPI GGTNACC 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 852
BstV1I GCAGC 5 cut(s) 47, 177, 202, 242, 661
BstX2I RGATCY 4 cut(s) 138, 712, 1030, 1121
BstYI RGATCY 4 cut(s) 138, 712, 1030, 1121
BsuRI GGCC 2 cut(s) 769, 850
BtsCI GGATG 2 cut(s) 425, 936
BtsI GCAGTG 1 cut(s) 907
BtsIMutI CAGTG 3 cut(s) 531, 890, 907
Cac8I GCNNGC 2 cut(s) 179, 861
Cfr10I RCCGGY 1 cut(s) 733
Cfr13I GGNCC 5 cut(s) 492, 767, 848, 849, 1018
Cfr9I CCCGGG 1 cut(s) 622
Csp6I GTAC 3 cut(s) 361, 922, 1059
CviAII CATG 3 cut(s) 696, 780, 983
CviQI GTAC 3 cut(s) 361, 922, 1059
DdeI CTNAG 2 cut(s) 289, 1010
DpnI GATC 6 cut(s) 140, 576, 714, 1015, 1032, 1123
DpnII GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
Eam1104I CTCTTC 2 cut(s) 56, 200
EarI CTCTTC 2 cut(s) 56, 200
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 272
Eco47I GGWCC 2 cut(s) 492, 1018
Eco57I CTGAAG 2 cut(s) 458, 938
Eco88I CYCGRG 1 cut(s) 622
Eco91I GGTNACC 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 720
EcoO109I RGGNCCY 2 cut(s) 848, 849
EcoO65I GGTNACC 1 cut(s) 442
EcoRII CCWGG 4 cut(s) 451, 640, 708, 956
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 852
ErhI CCWWGG 1 cut(s) 146
Esp3I CGTCTC 2 cut(s) 106, 900
FaeI CATG 3 cut(s) 699, 783, 986
FaqI GGGAC 4 cut(s) 309, 419, 572, 690
FatI CATG 3 cut(s) 695, 779, 982
FauI CCCGC 1 cut(s) 807
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 5 cut(s) 36, 191, 216, 231, 650
FokI GGATG 2 cut(s) 412, 943
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 5 cut(s) 36, 191, 216, 231, 650
GluI GCNGC 5 cut(s) 36, 191, 216, 231, 650
GsuI CTGGAG 1 cut(s) 1055
HaeIII GGCC 2 cut(s) 769, 850
HapII CCGG 8 cut(s) 327, 374, 403, 429, 590, 623, 734, 856
Hin1II CATG 3 cut(s) 699, 783, 986
HincII GTYRAC 3 cut(s) 109, 241, 472
HindII GTYRAC 3 cut(s) 109, 241, 472
HinfI GANTC 5 cut(s) 56, 457, 520, 542, 886
HpaII CCGG 8 cut(s) 327, 374, 403, 429, 590, 623, 734, 856
HphI GGTGA 4 cut(s) 287, 436, 638, 1147
Hpy166II GTNNAC 4 cut(s) 109, 241, 472, 1149
Hpy188I TCNGA 6 cut(s) 138, 292, 438, 918, 1018, 1121
Hpy188III TCNNGA 2 cut(s) 546, 1034
Hpy8I GTNNAC 4 cut(s) 109, 241, 472, 1149
HpyAV CCTTC 5 cut(s) 433, 647, 726, 839, 1103
HpyCH4III ACNGT 4 cut(s) 385, 487, 1063, 1146
HpyCH4IV ACGT 5 cut(s) 99, 120, 348, 582, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 856, 869
HpyF3I CTNAG 2 cut(s) 289, 1010
HpySE526I ACGT 5 cut(s) 99, 120, 348, 582, 743
Hsp92II CATG 3 cut(s) 699, 783, 986
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
LguI GCTCTTC 1 cut(s) 200
LmnI GCTCC 1 cut(s) 639
Lsp1109I GCAGC 5 cut(s) 47, 177, 202, 242, 661
LweI GCATC 2 cut(s) 190, 235
MaeII ACGT 5 cut(s) 99, 120, 348, 582, 743
MaeIII GTNAC 7 cut(s) 205, 442, 667, 746, 1038, 1063, 1140
MalI GATC 6 cut(s) 140, 576, 714, 1015, 1032, 1123
MboI GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
MboII GAAGA 7 cut(s) 33, 36, 43, 187, 379, 738, 1040
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 4 cut(s) 138, 712, 1030, 1121
MhlI GDGCHC 1 cut(s) 852
MluCI AATT 7 cut(s) 8, 165, 264, 595, 832, 895, 1157
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 6 cut(s) 70, 209, 212, 423, 505, 989
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 2 cut(s) 971, 987
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 8 cut(s) 327, 374, 403, 429, 590, 623, 734, 856
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 4 cut(s) 453, 642, 710, 958
MwoI GCNNNNNNNGC 3 cut(s) 187, 856, 869
NciI CCSGG 6 cut(s) 327, 404, 590, 623, 624, 856
NdeI CATATG 1 cut(s) 868
NdeII GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
NlaIII CATG 3 cut(s) 699, 783, 986
NlaIV GGNNCC 4 cut(s) 301, 714, 850, 851
NmuCI GTSAC 3 cut(s) 442, 746, 1140
NspI RCATGY 1 cut(s) 783
PasI CCCWGGG 1 cut(s) 452
PciSI GCTCTTC 1 cut(s) 200
PfeI GAWTC 4 cut(s) 56, 457, 520, 542
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 5 cut(s) 37, 192, 217, 232, 651
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
Ppu21I YACGTR 2 cut(s) 349, 744
Psp1406I AACGTT 1 cut(s) 582
Psp6I CCWGG 4 cut(s) 451, 640, 708, 956
PspEI GGTNACC 1 cut(s) 442
PspGI CCWGG 4 cut(s) 451, 640, 708, 956
PspN4I GGNNCC 4 cut(s) 301, 714, 850, 851
PspOMI GGGCCC 1 cut(s) 848
PspPI GGNCC 5 cut(s) 492, 767, 848, 849, 1018
PsuI RGATCY 4 cut(s) 138, 712, 1030, 1121
RsaI GTAC 3 cut(s) 362, 923, 1060
RsaNI GTAC 3 cut(s) 361, 922, 1059
RseI CAYNNNNRTG 2 cut(s) 971, 987
SalI GTCGAC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 200
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 5 cut(s) 36, 191, 216, 231, 650
Sau3AI GATC 6 cut(s) 138, 574, 712, 1013, 1030, 1121
Sau96I GGNCC 5 cut(s) 492, 767, 848, 849, 1018
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 1 cut(s) 880
SduI GDGCHC 1 cut(s) 852
SfaNI GCATC 2 cut(s) 190, 235
SinI GGWCC 2 cut(s) 492, 1018
SmaI CCCGGG 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 971, 987
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 7 cut(s) 8, 165, 264, 595, 832, 895, 1157
SsiI CCGC 3 cut(s) 213, 228, 800
SspI AATATT 1 cut(s) 787
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 4 cut(s) 385, 487, 1063, 1146
TaiI ACGT 5 cut(s) 102, 123, 351, 585, 746
TaqI TCGA 2 cut(s) 54, 471
TasI AATT 7 cut(s) 8, 165, 264, 595, 832, 895, 1157
TatI WGTACW 1 cut(s) 921
TfiI GAWTC 4 cut(s) 56, 457, 520, 542
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 531, 897, 907
TseFI GTSAC 3 cut(s) 442, 746, 1140
TseI GCWGC 5 cut(s) 35, 190, 215, 230, 649
Tsp45I GTSAC 3 cut(s) 442, 746, 1140
TspDTI ATGAA 5 cut(s) 33, 414, 947, 989, 999
TspMI CCCGGG 1 cut(s) 622
TspRI CASTG 3 cut(s) 531, 897, 907
VpaK11BI GGWCC 2 cut(s) 492, 1018
XagI CCTNNNNNAGG 1 cut(s) 720
XapI RAATTY 2 cut(s) 595, 832
XceI RCATGY 1 cut(s) 783
XcmI CCANNNNNNNNNTGG 1 cut(s) 1100
XmaI CCCGGG 1 cut(s) 622
XmiI GTMKAC 1 cut(s) 471
ZrmI AGTACT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.