pycom05g21200

Metal tolerance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
23888356 .. 23888700
345 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 345 bp
ATGCAGATTGCTTTGTACACAATAGGGAATTGGGCGAAAACAGTGATGGAAAATGTGTGGTCACTGATAGGGAAGACAGCACCAGCAGAGTACTTGGCAAAATTGACATACCTGATATGGAACCATGACAAGGAGCTCAAGCACATTGACAAGGTGAGAGCATACACTTTTGGCTGCAACTATTTTGTGGAGGCTGACATTGTCTTGCCCGGAGATATGTCTCTCAGCCACGCACACAACATCGGAGAGACGCTTCAGGAAAAGATCGAAAACCTTCCTGAGGTGGAACGAGCTTTCGTTCATGTGAGACTACTCACAGGCATGAGCACAACAATGCTAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_efflux ZT_dimer

Protein Analysis

115

Amino Acids

13.0

Weight (kDa)

5.91

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ZT_dimer PF16916 34 - 102 7.5e-09 Cation efflux protein, cytoplasmic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 239
AfaI GTAC 2 cut(s) 17, 92
AfiI CCNNNNNNNGG 2 cut(s) 130, 280
AluBI AGCT 2 cut(s) 136, 293
AluI AGCT 2 cut(s) 136, 293
Alw21I GWGCWC 2 cut(s) 138, 329
Alw26I GTCTC 3 cut(s) 225, 242, 301
ApeKI GCWGC 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 166
AxyI CCTNAGG 1 cut(s) 279
BanII GRGCYC 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 2 cut(s) 138, 329
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 40
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 3 cut(s) 225, 242, 301
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
BmcAI AGTACT 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 210
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 280
Bse21I CCTNAGG 1 cut(s) 279
BseLI CCNNNNNNNGG 2 cut(s) 130, 280
BseMII CTCAG 2 cut(s) 238, 270
BseXI GCAGC 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 138, 329
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 2 cut(s) 130, 280
BsmAI GTCTC 3 cut(s) 225, 242, 301
BsmBI CGTCTC 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 138, 329
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 237, 271
BspLI GGNNCC 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 15
BssMI GATC 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 43
BstAUI TGTACA 1 cut(s) 15
BstDEI CTNAG 3 cut(s) 224, 279, 338
BstENI CCTNNNNNAGG 1 cut(s) 278
BstKTI GATC 1 cut(s) 267
BstMAI GTCTC 3 cut(s) 225, 242, 301
BstMBI GATC 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 208
BstV1I GCAGC 1 cut(s) 161
BstV2I GAAGAC 1 cut(s) 80
Bsu36I CCTNAGG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 48, 62
CseI GACGC 1 cut(s) 259
Csp6I GTAC 2 cut(s) 16, 91
CviAII CATG 3 cut(s) 125, 302, 322
CviJI RGCY 5 cut(s) 136, 174, 194, 228, 293
CviKI_1 RGCY 5 cut(s) 136, 174, 194, 228, 293
CviQI GTAC 2 cut(s) 16, 91
DdeI CTNAG 3 cut(s) 224, 279, 338
DpnI GATC 1 cut(s) 266
DpnII GATC 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 239
Eco81I CCTNAGG 1 cut(s) 279
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 138
Esp3I CGTCTC 1 cut(s) 242
FaeI CATG 3 cut(s) 128, 305, 325
FaiI YATR 7 cut(s) 109, 118, 126, 163, 218, 303, 323
FatI CATG 3 cut(s) 124, 301, 321
Fnu4HI GCNGC 1 cut(s) 175
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 175
GluI GCNGC 1 cut(s) 175
HapII CCGG 1 cut(s) 210
HgaI GACGC 1 cut(s) 259
Hin1II CATG 3 cut(s) 128, 305, 325
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 18
Hpy188I TCNGA 1 cut(s) 245
Hpy188III TCNNGA 2 cut(s) 257, 278
Hpy8I GTNNAC 1 cut(s) 18
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4V TGCA 2 cut(s) 4, 177
HpyF3I CTNAG 3 cut(s) 224, 279, 338
Hsp92II CATG 3 cut(s) 128, 305, 325
Kzo9I GATC 1 cut(s) 264
LmnI GCTCC 1 cut(s) 133
LpnPI CCDG 6 cut(s) 96, 125, 223, 242, 291, 303
Lsp1109I GCAGC 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 266
MboI GATC 1 cut(s) 264
MboII GAAGA 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 138, 329
MluCI AATT 2 cut(s) 28, 101
MnlI CCTC 2 cut(s) 184, 274
MroXI GAANNNNTTC 1 cut(s) 273
MslI CAYNNNNRTG 2 cut(s) 320, 332
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 210
NciI CCSGG 1 cut(s) 210
NdeII GATC 1 cut(s) 264
NlaIII CATG 3 cut(s) 128, 305, 325
NlaIV GGNNCC 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 273
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 176
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 2 cut(s) 17, 92
RsaNI GTAC 2 cut(s) 16, 91
RseI CAYNNNNRTG 2 cut(s) 320, 332
SacI GAGCTC 1 cut(s) 138
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 1 cut(s) 264
ScaI AGTACT 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 2 cut(s) 138, 329
SetI ASST 6 cut(s) 114, 138, 156, 276, 285, 295
SmiMI CAYNNNNRTG 2 cut(s) 320, 332
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 2 cut(s) 28, 101
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 267
TasI AATT 2 cut(s) 28, 101
TatI WGTACW 2 cut(s) 15, 90
TscAI CASTG 2 cut(s) 48, 69
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 1 cut(s) 174
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 2 cut(s) 48, 69
Tth111I GACNNNGTC 1 cut(s) 200
XagI CCTNNNNNAGG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 273
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.