pycom05g22770

DNA excision repair protein ERCC-6-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
24967184 .. 24968535
1352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1149 bp
ATGAATGTTAACAATCGAATTCGGGTATCGAAGTTTAGAATTAATCTGTTGACATATAACAATGTGCAATTACATAAGAAAAGTTTCCCGGCATTCATCACAACGTGTAAAACAAAATTAACAAGCATGTATAGACTAAACTCGATTCCACAAAAGTCGTGTTTCTTATCTGTCTTTTTCAATTCAAGCAATCTTGTTCCTTTCCCATCAGACATCCAAGTTTTCCATCTGAATGTTGCTCTTGAGAGGCACATACTCTCCGAGATACCCGCCCAGATGATCGTGCAGAGCGCCCTTGTCAATCCCCTGGTGATGGTAGCTTTTGCAGACGTACACCAGGACGCACACCAAAAACCCACCAACCACAATCCTCACAAAAACCCCATAATTCTCCCCGCCGTGCACCACCACCGACCCGAAAACCGCACTGATAACCCCGCAGATGGTCAAGTACCCGAAAACGAGCACGAAGGCCATCCCAACCTTCCCCTTGAGCAGCTCATAGCTCTTCTTCATGGCGGCGAACCCGTAGACGGGCTCGAGCACGGAGACCACGCTGGCCAAGTGCCAGAGCGCGGTGATGTAGACATGGACGACCAAGAAGAGGAGGAAGATGACAATGCCGGAGAAGAGGAGCAGAAAGGAATTCTGGGTATCGATGGCGAGGATGAGGAGGACGAGGAAGCCGACGAAGACGAGATTGTAGCAGAACATGAGGAGGGAGACCCAGAGGAAAGTGATGAAGAGGCGCTTGAAGACTTTTGGGATTGCGGAGAGGGTGTTGGAGAAGGAGACGGGCTTGGAGGTGTAGAGGGAGGCGGCGGTGAAGACGACGGCGGCGGTGGAGAGGAGGGAGAAGGCGAAGAGGAAGATGAGGTAGCAGAATTGGAAGAGGAGGAGCAGGGTCCATTCGTGGTGGAGCTGGGCGGTGTCGGTGGAAGGGCCTTGGAGCTGGTTGAGGAGAGGGTGGGTGAAGAGGGAAAGGTTTTGGGGGACTGTTTGGGGATGGAGGTGGATTCTCTGAGGATGTCAGGGATGGTGAGAAATTGGAGCTCCTCTGGGGCCAGATCCATTCGGATTTGGAGGGTCTGTTTGGACGAAAACAAAAGAAAAGTTGGGTTTTTGATTGATGGGGTGTTTGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

383

Amino Acids

42.09

Weight (kDa)

4.32

Isoelectric Point (pI)

51.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 531, 585
AccII CGCG 1 cut(s) 576
AclWI GGATC 1 cut(s) 1062
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 2 cut(s) 18, 645
AdeI CACNNNGTG 1 cut(s) 105
AfaI GTAC 2 cut(s) 333, 453
AfiI CCNNNNNNNGG 6 cut(s) 313, 443, 533, 534, 575, 604
AflIII ACRYGT 1 cut(s) 104
AgsI TTSAA 3 cut(s) 181, 186, 755
AjnI CCWGG 2 cut(s) 306, 336
AluBI AGCT 6 cut(s) 320, 499, 506, 922, 952, 1053
AluI AGCT 6 cut(s) 320, 499, 506, 922, 952, 1053
Alw21I GWGCWC 4 cut(s) 405, 468, 546, 1055
Alw26I GTCTC 3 cut(s) 543, 717, 786
Alw44I GTGCAC 1 cut(s) 401
AlwI GGATC 1 cut(s) 1062
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 4 cut(s) 472, 559, 942, 1062
ApaLI GTGCAC 1 cut(s) 401
ApeKI GCWGC 1 cut(s) 496
ApoI RAATTY 2 cut(s) 18, 645
AseI ATTAAT 1 cut(s) 42
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 3 cut(s) 293, 576, 751
AspS9I GGNCC 3 cut(s) 905, 942, 1062
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 322, 590, 836, 983, 1051
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 905
BaeGI GKGCMC 1 cut(s) 405
BalI TGGCCA 1 cut(s) 561
BanII GRGCYC 2 cut(s) 540, 1055
BbsI GAAGAC 3 cut(s) 699, 762, 834
Bbv12I GWGCWC 4 cut(s) 405, 468, 546, 1055
BbvI GCAGC 1 cut(s) 508
BccI CCATC 9 cut(s) 214, 234, 307, 437, 483, 653, 1000, 1030, 1124
BceAI ACGGC 2 cut(s) 383, 850
BciT130I CCWGG 2 cut(s) 308, 338
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 3 cut(s) 543, 717, 786
BfoI RGCGCY 2 cut(s) 294, 752
BisI GCNGC 4 cut(s) 497, 520, 820, 838
BlsI GCNGC 4 cut(s) 498, 521, 821, 839
Bme1390I CCNGG 3 cut(s) 89, 308, 338
Bme18I GGWCC 1 cut(s) 905
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 3 cut(s) 905, 942, 1062
BmiI GGNNCC 2 cut(s) 906, 1063
BmrFI CCNGG 3 cut(s) 89, 308, 338
BpiI GAAGAC 3 cut(s) 699, 762, 834
BpuEI CTTGAG 2 cut(s) 263, 512
BpuMI CCSGG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 657
BsaI GGTCTC 2 cut(s) 543, 717
BsaJI CCNNGG 2 cut(s) 306, 945
BsaXI ACNNNNNCTCC 6 cut(s) 242, 272, 765, 795, 1075, 1105
Bsc4I CCNNNNNNNGG 6 cut(s) 313, 443, 533, 534, 575, 604
BseBI CCWGG 2 cut(s) 308, 338
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 2 cut(s) 306, 945
BseGI GGATG 6 cut(s) 213, 475, 673, 1011, 1032, 1041
BseLI CCNNNNNNNGG 6 cut(s) 313, 443, 533, 534, 575, 604
BseMII CTCAG 1 cut(s) 1013
BseRI GAGGAG 9 cut(s) 620, 647, 686, 731, 863, 908, 911, 974, 1045
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 1 cut(s) 508
BseYI CCCAGC 1 cut(s) 922
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 4 cut(s) 474, 561, 944, 1064
BshVI ATCGAT 1 cut(s) 657
BsiHKAI GWGCWC 4 cut(s) 405, 468, 546, 1055
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 89, 624
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 6 cut(s) 313, 443, 533, 534, 575, 604
BsmAI GTCTC 3 cut(s) 543, 717, 786
BsmBI CGTCTC 1 cut(s) 786
BsmFI GGGAC 1 cut(s) 1007
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 4 cut(s) 474, 561, 944, 1064
Bso31I GGTCTC 2 cut(s) 543, 717
BsoBI CYCGRG 1 cut(s) 539
Bsp1286I GDGCHC 5 cut(s) 405, 468, 540, 546, 1055
Bsp143I GATC 2 cut(s) 279, 1067
BspANI GGCC 4 cut(s) 474, 561, 944, 1064
BspCNI CTCAG 1 cut(s) 1014
BspDI ATCGAT 1 cut(s) 657
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 906, 1063
BspPI GGATC 1 cut(s) 1062
BspQI GCTCTTC 1 cut(s) 513
BspTNI GGTCTC 2 cut(s) 543, 717
BssECI CCNNGG 2 cut(s) 306, 945
BssMI GATC 2 cut(s) 279, 1067
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 2 cut(s) 308, 338
Bst4CI ACNGT 1 cut(s) 998
Bst6I CTCTTC 7 cut(s) 513, 597, 624, 738, 858, 885, 969
BstC8I GCNNGC 1 cut(s) 559
BstDEI CTNAG 1 cut(s) 1022
BstF5I GGATG 6 cut(s) 213, 475, 673, 1011, 1032, 1041
BstFNI CGCG 1 cut(s) 576
BstH2I RGCGCY 2 cut(s) 294, 752
BstHHI GCGC 3 cut(s) 293, 576, 751
BstKTI GATC 2 cut(s) 282, 1070
BstMAI GTCTC 3 cut(s) 543, 717, 786
BstMBI GATC 2 cut(s) 279, 1067
BstNI CCWGG 2 cut(s) 308, 338
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 3 cut(s) 87, 306, 336
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 1 cut(s) 508
BstV2I GAAGAC 3 cut(s) 699, 762, 834
BstX2I RGATCY 1 cut(s) 1067
BstYI RGATCY 1 cut(s) 1067
Bsu15I ATCGAT 1 cut(s) 657
BsuRI GGCC 4 cut(s) 474, 561, 944, 1064
BsuTUI ATCGAT 1 cut(s) 657
BtsCI GGATG 6 cut(s) 213, 475, 673, 1011, 1032, 1041
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 1 cut(s) 559
CfoI GCGC 3 cut(s) 293, 576, 751
Cfr13I GGNCC 3 cut(s) 905, 942, 1062
ClaI ATCGAT 1 cut(s) 657
CseI GACGC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 332, 452
CviAII CATG 4 cut(s) 127, 515, 589, 713
CviQI GTAC 2 cut(s) 332, 452
DdeI CTNAG 1 cut(s) 1022
DpnI GATC 2 cut(s) 281, 1069
DpnII GATC 2 cut(s) 279, 1067
DraIII CACNNNGTG 1 cut(s) 105
EaeI YGGCCR 1 cut(s) 559
Eam1104I CTCTTC 7 cut(s) 513, 597, 624, 738, 858, 885, 969
EarI CTCTTC 7 cut(s) 513, 597, 624, 738, 858, 885, 969
Ecl136II GAGCTC 1 cut(s) 1053
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 2 cut(s) 540, 1055
Eco31I GGTCTC 2 cut(s) 543, 717
Eco47I GGWCC 1 cut(s) 905
Eco53kI GAGCTC 1 cut(s) 1053
Eco88I CYCGRG 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 1053
EcoO109I RGGNCCY 1 cut(s) 942
EcoRI GAATTC 2 cut(s) 18, 645
EcoRII CCWGG 2 cut(s) 306, 336
EcoT14I CCWWGG 1 cut(s) 945
EcoT38I GRGCYC 2 cut(s) 540, 1055
ErhI CCWWGG 1 cut(s) 945
Esp3I CGTCTC 1 cut(s) 786
FaeI CATG 4 cut(s) 130, 518, 592, 716
FalI AAGNNNNNCTT 2 cut(s) 735, 767
FaqI GGGAC 1 cut(s) 1007
FatI CATG 4 cut(s) 126, 514, 588, 712
FauI CCCGC 3 cut(s) 277, 403, 445
FblI GTMKAC 2 cut(s) 531, 585
Fnu4HI GCNGC 4 cut(s) 497, 520, 820, 838
FokI GGATG 6 cut(s) 200, 462, 680, 1018, 1039, 1048
FriOI GRGCYC 2 cut(s) 540, 1055
Fsp4HI GCNGC 4 cut(s) 497, 520, 820, 838
GlaI GCGC 3 cut(s) 292, 575, 750
GluI GCNGC 4 cut(s) 497, 520, 820, 838
GsaI CCCAGC 1 cut(s) 926
HaeII RGCGCY 2 cut(s) 294, 752
HaeIII GGCC 4 cut(s) 474, 561, 944, 1064
HapII CCGG 2 cut(s) 89, 624
HgaI GACGC 1 cut(s) 350
HhaI GCGC 3 cut(s) 293, 576, 751
Hin1II CATG 4 cut(s) 130, 518, 592, 716
Hin6I GCGC 3 cut(s) 291, 574, 749
HinP1I GCGC 3 cut(s) 291, 574, 749
HincII GTYRAC 2 cut(s) 10, 51
HindII GTYRAC 2 cut(s) 10, 51
HinfI GANTC 2 cut(s) 145, 1016
HpaI GTTAAC 1 cut(s) 10
HpaII CCGG 2 cut(s) 89, 624
HphI GGTGA 5 cut(s) 322, 590, 836, 983, 1051
Hpy166II GTNNAC 6 cut(s) 10, 51, 334, 403, 532, 586
Hpy188I TCNGA 5 cut(s) 211, 231, 262, 1023, 1077
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 6 cut(s) 10, 51, 334, 403, 532, 586
Hpy99I CGWCG 2 cut(s) 692, 836
HpyAV CCTTC 5 cut(s) 464, 494, 782, 851, 933
HpyCH4III ACNGT 1 cut(s) 998
HpyCH4IV ACGT 2 cut(s) 104, 330
HpyCH4V TGCA 4 cut(s) 67, 286, 326, 403
HpyF3I CTNAG 1 cut(s) 1022
HpySE526I ACGT 2 cut(s) 104, 330
Hsp92II CATG 4 cut(s) 130, 518, 592, 716
HspAI GCGC 3 cut(s) 291, 574, 749
KspAI GTTAAC 1 cut(s) 10
Kzo9I GATC 2 cut(s) 279, 1067
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 6 cut(s) 634, 898, 919, 949, 1050, 1058
Lsp1109I GCAGC 1 cut(s) 508
MaeII ACGT 2 cut(s) 104, 330
MalI GATC 2 cut(s) 281, 1069
MboI GATC 2 cut(s) 279, 1067
MflI RGATCY 1 cut(s) 1067
MhlI GDGCHC 5 cut(s) 405, 468, 540, 546, 1055
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 9 cut(s) 18, 39, 68, 116, 181, 387, 645, 884, 1045
MluNI TGGCCA 1 cut(s) 561
MmeI TCCRAC 1 cut(s) 763
Mox20I TGGCCA 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 561
MseI TTAA 3 cut(s) 9, 42, 119
MslI CAYNNNNRTG 1 cut(s) 231
Msp20I TGGCCA 1 cut(s) 561
MspI CCGG 2 cut(s) 89, 624
MspR9I CCNGG 3 cut(s) 89, 308, 338
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 2 cut(s) 308, 338
MvnI CGCG 1 cut(s) 576
NciI CCSGG 1 cut(s) 89
NdeII GATC 2 cut(s) 279, 1067
NlaIII CATG 4 cut(s) 130, 518, 592, 716
NlaIV GGNNCC 2 cut(s) 906, 1063
NspI RCATGY 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 2 cut(s) 145, 1016
PkrI GCNGC 4 cut(s) 498, 521, 821, 839
PshBI ATTAAT 1 cut(s) 42
Psp124BI GAGCTC 1 cut(s) 1055
Psp6I CCWGG 2 cut(s) 306, 336
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 2 cut(s) 306, 336
PspN4I GGNNCC 2 cut(s) 906, 1063
PspPI GGNCC 3 cut(s) 905, 942, 1062
PspXI VCTCGAGB 1 cut(s) 539
PsuI RGATCY 1 cut(s) 1067
RsaI GTAC 2 cut(s) 333, 453
RsaNI GTAC 2 cut(s) 332, 452
RseI CAYNNNNRTG 1 cut(s) 231
SacI GAGCTC 1 cut(s) 1055
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 3 cut(s) 9, 42, 119
SatI GCNGC 4 cut(s) 497, 520, 820, 838
Sau3AI GATC 2 cut(s) 279, 1067
Sau96I GGNCC 3 cut(s) 905, 942, 1062
ScrFI CCNGG 3 cut(s) 89, 308, 338
SduI GDGCHC 5 cut(s) 405, 468, 540, 546, 1055
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 1 cut(s) 905
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 3 cut(s) 242, 491, 539
SmoI CTYRAG 3 cut(s) 242, 491, 539
Sse9I AATT 9 cut(s) 18, 39, 68, 116, 181, 387, 645, 884, 1045
SstI GAGCTC 1 cut(s) 1055
StyD4I CCNGG 3 cut(s) 87, 306, 336
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 1 cut(s) 998
TaiI ACGT 2 cut(s) 107, 333
TaqI TCGA 5 cut(s) 16, 29, 143, 540, 657
TasI AATT 9 cut(s) 18, 39, 68, 116, 181, 387, 645, 884, 1045
TauI GCSGC 3 cut(s) 522, 822, 840
TfiI GAWTC 2 cut(s) 145, 1016
Tru1I TTAA 3 cut(s) 9, 42, 119
Tru9I TTAA 3 cut(s) 9, 42, 119
TscAI CASTG 1 cut(s) 433
TseI GCWGC 1 cut(s) 496
TspDTI ATGAA 4 cut(s) 17, 85, 503, 756
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 1 cut(s) 433
VneI GTGCAC 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 905
VspI ATTAAT 1 cut(s) 42
XapI RAATTY 2 cut(s) 18, 645
XceI RCATGY 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 539
XmiI GTMKAC 2 cut(s) 531, 585
XmnI GAANNNNTTC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.