pycom05g22860

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
25023534 .. 25025804
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1653 bp
ATGTATCCAAGTGTAAATCCGTATATACTACTGGCTCGTCGTCAATCTTTCAAATACACGCTTGTATGCACAAGTAACAAAAGAGAAATGAGACAAAGTAGCATTATCATCTTCTTCTTCCAATTGAAAGCCATGGATCCGGAACAGAAAAGAACGGTTTTGGAGGACTTGGACATGTTCGTTGAGAGCAAAGAGTACTACCAAAGGGTGGGATGGGCTTGGAAGCAAGGGTACCTTCTATATAGTCCTCCAGGCACTGAAAAATCGCCCCTAGTAGCTGCAATGGCTAATTACCTCGAGTTCGAAGTCTACGATTTGCATCTCTCACATTTGAAAACTGATTTGGACATAAAATATAAGCACAAAAACTGGTCAGAAGGTGGTCAGGTTAGTATTCTGCCACAAGAGATTTGCTTTAAATTTGATGTTTTAACTGCATGTCCGATTTCTTTTTGTGCGCAGCTTACCCTATCCGATCTACTAAACTTCACAGATGGGCTGTCGCCAAAGCAAGCTATGCACATTGCACATCCACACCTCTTAATGCACGTACGAGGGTTTCAAAACTTAATTTGGCTTCTATTTACCTCAAAATTTATGAAGACCACCCCTTGTTTGAAGAGATTAAAACCTTACTGGAAAACACAAAGGTCACTCCTGCACTCACGATTAACTGAGTTGAACTTCAGCATTCATCAGCCACATTTCATTTCTACTGATTTGATACTGTTCTTTCATTTATCTAGGAGCCAAAAGTCTCACGACAAAACTCAAAGCATGTTTGGTATTTTATTTCAATCCAATTTTTTAAACTCAAAAACAATTTTTAAGTTAGTTTTTAAACTTAAACTCACTCATTTTTTTTTTCCTCCTCCCCTCTCACTCCAAAGCTATCTCTCTCTTTTCTTCTTTCTCTCTACCCTTCTCCTTTATTTTTTTTACCTTTTTCGTCTCTCCTCCAATCCGCTCTCATTATTTTCTCGGCTTTTTTCTATCACTCATCTTCCTCTCTATCTCCTCCGATCCTCTCACTTCTTTCTCTTTTCTCCAATTATCTTTTACTTTCTTTCCTCTTCTCTCCTCTTTCTCTCTCAACCCTCTCTTTTTAGATCTCTTTATCCATTTTTGAGTTTTAAAGGAAATTCTGTACTTTCATTTGTTTGCAATCGTAAATGTAAGCCTCAAATGTCGGATAAGGACGCTGAGCTTCCAATGTGCATACCTTTGAAGATGTTTGATGACAAAACAATATTTGGTACTTTAAACACAGGGAAAACCTTAACTTTTCCGATATTAAACATGACGAGCATTCCCATGTGGCCGGAGGGATATGCCTTGATAAGAAATTTGAGATGCAGAATTAATTTTCATCCAGTTCATTATATTCGAACGTCGACTAAGCAGGTCAAGGTTGTCACCATCTTCTCCTTTGAAGAGAGTGTTCTTGTAAGGTTTACCACATTTGCACAAAAATTTCATCCCGTCTTTGTTTTTTTAATTAAGGTTTACCATGTTTACACAGAAAGAAATTCTCTGTCGACAGAAGTGCCAACTTTCGGGATATTGATTCGAGACAACATAGTGCCACTTGTTCTGTATGATGACATAAACTGGATCTCATCCTGTCTTGGGCGAGCTTTGGTAGGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA AAA_assoc

Protein Analysis

551

Amino Acids

64.42

Weight (kDa)

9.58

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 459
Acc36I ACCTGC 1 cut(s) 1393
Acc65I GGTACC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 231
AccB7I CCANNNNNTGG 1 cut(s) 208
AccBSI CCGCTC 1 cut(s) 967
AccI GTMKAC 3 cut(s) 309, 1394, 1538
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 1 cut(s) 965
AclWI GGATC 4 cut(s) 131, 144, 1017, 1622
AcoI YGGCCR 1 cut(s) 1319
AcsI RAATTY 6 cut(s) 419, 593, 1141, 1345, 1472, 1528
AcuI CTGAAG 1 cut(s) 670
AfaI GTAC 5 cut(s) 197, 233, 552, 1149, 1258
AfiI CCNNNNNNNGG 3 cut(s) 208, 1556, 1629
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 9 cut(s) 52, 127, 334, 563, 619, 682, 797, 1228, 1433
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 6 cut(s) 278, 463, 515, 891, 1207, 1637
AluI AGCT 6 cut(s) 278, 463, 515, 891, 1207, 1637
Alw26I GTCTC 4 cut(s) 85, 762, 956, 1566
AlwI GGATC 4 cut(s) 131, 144, 1017, 1622
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 1319
ApeKI GCWGC 2 cut(s) 278, 460
ApoI RAATTY 6 cut(s) 419, 593, 1141, 1345, 1472, 1528
AseI ATTAAT 1 cut(s) 1362
Asp718I GGTACC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 460
AsuHPI GGTGA 1 cut(s) 1408
AsuII TTCGAA 2 cut(s) 303, 1387
AvaI CYCGRG 1 cut(s) 296
BamHI GGATCC 1 cut(s) 136
BanI GGYRCC 1 cut(s) 231
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 2 cut(s) 265, 472
BccI CCATC 3 cut(s) 207, 488, 1427
BcgI CGANNNNNNTGC 2 cut(s) 1528, 1562
BciT130I CCWGG 1 cut(s) 252
BciVI GTATCC 1 cut(s) 15
BcoDI GTCTC 4 cut(s) 85, 762, 956, 1566
BfaI CTAG 2 cut(s) 272, 744
BfuAI ACCTGC 1 cut(s) 1393
BfuI GTATCC 1 cut(s) 15
BglII AGATCT 1 cut(s) 1109
BisI GCNGC 2 cut(s) 279, 461
BlpI GCTNAGC 1 cut(s) 1203
BlsI GCNGC 2 cut(s) 280, 462
BmcAI AGTACT 1 cut(s) 197
Bme1390I CCNGG 1 cut(s) 252
BmeT110I CYCGRG 1 cut(s) 296
BmiI GGNNCC 3 cut(s) 138, 233, 749
BmrFI CCNGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 328, 1343
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 1203
Bpu14I TTCGAA 2 cut(s) 303, 1387
BsaAI YACGTR 1 cut(s) 550
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 1556, 1629
Bse1I ACTGG 5 cut(s) 36, 374, 641, 1373, 1616
Bse3DI GCAATG 2 cut(s) 288, 522
Bse8I GATNNNNATC 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 5 cut(s) 218, 529, 1369, 1477, 1619
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 208, 1556, 1629
BseMI GCAATG 2 cut(s) 288, 522
BseMII CTCAG 2 cut(s) 666, 1194
BseNI ACTGG 5 cut(s) 36, 374, 641, 1373, 1616
BseRI GAGGAG 4 cut(s) 861, 946, 1007, 1070
BseXI GCAGC 2 cut(s) 265, 472
BsgI GTGCAG 1 cut(s) 644
BshFI GGCC 1 cut(s) 1321
BshNI GGYRCC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 140, 1322
BsiWI CGTACG 1 cut(s) 550
BslI CCNNNNNNNGG 3 cut(s) 208, 1556, 1629
BsmAI GTCTC 4 cut(s) 85, 762, 956, 1566
BsmBI CGTCTC 1 cut(s) 956
BsmI GAATGC 2 cut(s) 690, 1308
BsnI GGCC 1 cut(s) 1321
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 2 cut(s) 303, 1387
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 5 cut(s) 136, 475, 1022, 1109, 1614
Bsp1720I GCTNAGC 1 cut(s) 1203
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 1 cut(s) 965
BspANI GGCC 1 cut(s) 1321
BspCNI CTCAG 2 cut(s) 667, 1195
BspEI TCCGGA 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 138, 233, 749
BspMI ACCTGC 1 cut(s) 1393
BspPI GGATC 4 cut(s) 131, 144, 1017, 1622
BspT104I TTCGAA 2 cut(s) 303, 1387
BspT107I GGYRCC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 967
BsrDI GCAATG 2 cut(s) 288, 522
BsrI ACTGG 5 cut(s) 36, 374, 641, 1373, 1616
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 5 cut(s) 136, 475, 1022, 1109, 1614
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 157, 729
Bst6I CTCTTC 3 cut(s) 614, 1078, 1428
BstAPI GCANNNNNTGC 1 cut(s) 517
BstBAI YACGTR 1 cut(s) 550
BstBI TTCGAA 2 cut(s) 303, 1387
BstC8I GCNNGC 2 cut(s) 513, 1635
BstDEI CTNAG 3 cut(s) 675, 1203, 1398
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 5 cut(s) 218, 529, 1369, 1477, 1619
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 5 cut(s) 139, 478, 1025, 1112, 1617
BstMAI GTCTC 4 cut(s) 85, 762, 956, 1566
BstMBI GATC 5 cut(s) 136, 475, 1022, 1109, 1614
BstMWI GCNNNNNNNGC 2 cut(s) 284, 517
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 3 cut(s) 178, 441, 781
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 265, 472
BstV2I GAAGAC 1 cut(s) 608
BstX2I RGATCY 3 cut(s) 136, 1109, 1614
BstYI RGATCY 3 cut(s) 136, 1109, 1614
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 1321
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 5 cut(s) 218, 529, 1369, 1477, 1619
BtsIMutI CAGTG 1 cut(s) 255
BveI ACCTGC 1 cut(s) 1393
Cac8I GCNNGC 2 cut(s) 513, 1635
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 460
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 5 cut(s) 196, 232, 551, 1148, 1257
CviAII CATG 7 cut(s) 133, 175, 438, 778, 1300, 1315, 1511
CviQI GTAC 5 cut(s) 196, 232, 551, 1148, 1257
DdeI CTNAG 3 cut(s) 675, 1203, 1398
DpnI GATC 5 cut(s) 138, 477, 1024, 1111, 1616
DpnII GATC 5 cut(s) 136, 475, 1022, 1109, 1614
DraI TTTAAA 5 cut(s) 418, 810, 841, 1135, 1263
EaeI YGGCCR 1 cut(s) 1319
Eam1104I CTCTTC 3 cut(s) 614, 1078, 1428
EarI CTCTTC 3 cut(s) 614, 1078, 1428
Eco130I CCWWGG 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 670
Eco88I CYCGRG 1 cut(s) 296
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 956
FaeI CATG 7 cut(s) 136, 178, 441, 781, 1303, 1318, 1514
FalI AAGNNNNNCTT 2 cut(s) 219, 251
FatI CATG 7 cut(s) 132, 174, 437, 777, 1299, 1314, 1510
FblI GTMKAC 3 cut(s) 309, 1394, 1538
Fnu4HI GCNGC 2 cut(s) 279, 461
FokI GGATG 5 cut(s) 225, 516, 1356, 1464, 1606
Fsp4HI GCNGC 2 cut(s) 279, 461
FspBI CTAG 2 cut(s) 272, 744
FspI TGCGCA 1 cut(s) 459
GlaI GCGC 1 cut(s) 459
GluI GCNGC 2 cut(s) 279, 461
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 1 cut(s) 1321
HapII CCGG 2 cut(s) 140, 1322
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 1 cut(s) 460
Hin1II CATG 7 cut(s) 136, 178, 441, 781, 1303, 1318, 1514
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 2 cut(s) 1395, 1539
HindII GTYRAC 2 cut(s) 1395, 1539
HinfI GANTC 1 cut(s) 1567
HpaII CCGG 2 cut(s) 140, 1322
HphI GGTGA 1 cut(s) 1408
Hpy166II GTNNAC 6 cut(s) 310, 1395, 1455, 1507, 1516, 1539
Hpy188I TCNGA 6 cut(s) 376, 444, 475, 1022, 1192, 1290
Hpy188III TCNNGA 5 cut(s) 140, 666, 761, 1558, 1571
Hpy8I GTNNAC 6 cut(s) 310, 1395, 1455, 1507, 1516, 1539
Hpy99I CGWCG 2 cut(s) 42, 1396
HpyAV CCTTC 3 cut(s) 245, 371, 932
HpyCH4III ACNGT 2 cut(s) 157, 729
HpyCH4IV ACGT 2 cut(s) 549, 1391
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 517
HpyF3I CTNAG 3 cut(s) 675, 1203, 1398
HpySE526I ACGT 2 cut(s) 549, 1391
Hsp92II CATG 7 cut(s) 136, 178, 441, 781, 1303, 1318, 1514
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 139
KpnI GGTACC 1 cut(s) 235
Kzo9I GATC 5 cut(s) 136, 475, 1022, 1109, 1614
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 2 cut(s) 265, 472
LweI GCATC 2 cut(s) 328, 1343
MaeI CTAG 2 cut(s) 272, 744
MaeII ACGT 2 cut(s) 549, 1391
MaeIII GTNAC 3 cut(s) 74, 651, 1414
MalI GATC 5 cut(s) 138, 477, 1024, 1111, 1616
MbiI CCGCTC 1 cut(s) 967
MboI GATC 5 cut(s) 136, 475, 1022, 1109, 1614
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 3 cut(s) 136, 1109, 1614
MmeI TCCRAC 1 cut(s) 1170
MroI TCCGGA 1 cut(s) 139
MslI CAYNNNNRTG 1 cut(s) 1313
MspI CCGG 2 cut(s) 140, 1322
MspR9I CCNGG 1 cut(s) 252
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 2 cut(s) 690, 1308
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 2 cut(s) 284, 517
NcoI CCATGG 1 cut(s) 132
NdeII GATC 5 cut(s) 136, 475, 1022, 1109, 1614
NlaIII CATG 7 cut(s) 136, 178, 441, 781, 1303, 1318, 1514
NlaIV GGNNCC 3 cut(s) 138, 233, 749
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 2 cut(s) 651, 1414
NsbI TGCGCA 1 cut(s) 459
NspI RCATGY 3 cut(s) 178, 441, 781
NspV TTCGAA 2 cut(s) 303, 1387
PacI TTAATTAA 1 cut(s) 1500
PaeR7I CTCGAG 1 cut(s) 296
PciI ACATGT 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 2 cut(s) 690, 1308
PfeI GAWTC 1 cut(s) 1567
Pfl23II CGTACG 1 cut(s) 550
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 2 cut(s) 280, 462
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 174
PshBI ATTAAT 1 cut(s) 1362
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspLI CGTACG 1 cut(s) 550
PspN4I GGNNCC 3 cut(s) 138, 233, 749
PspXI VCTCGAGB 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 136, 1109, 1614
RsaI GTAC 5 cut(s) 197, 233, 552, 1149, 1258
RsaNI GTAC 5 cut(s) 196, 232, 551, 1148, 1257
RseI CAYNNNNRTG 1 cut(s) 1313
SalI GTCGAC 2 cut(s) 1393, 1537
SatI GCNGC 2 cut(s) 279, 461
Sau3AI GATC 5 cut(s) 136, 475, 1022, 1109, 1614
ScaI AGTACT 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 252
SfaNI GCATC 2 cut(s) 328, 1343
Sfr274I CTCGAG 1 cut(s) 296
SfuI TTCGAA 2 cut(s) 303, 1387
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SsiI CCGC 1 cut(s) 965
SspI AATATT 1 cut(s) 1251
SspMI CTAG 2 cut(s) 272, 744
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 157, 729
TaiI ACGT 2 cut(s) 552, 1394
TaqI TCGA 6 cut(s) 297, 303, 1387, 1394, 1538, 1570
TatI WGTACW 2 cut(s) 195, 1147
TfiI GAWTC 1 cut(s) 1567
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 651, 1414
TseI GCWGC 2 cut(s) 278, 460
Tsp45I GTSAC 2 cut(s) 651, 1414
TspDTI ATGAA 8 cut(s) 614, 683, 697, 725, 1143, 1358, 1367, 1466
TspGWI ACGGA 1 cut(s) 9
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 208
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 6 cut(s) 419, 593, 1141, 1345, 1472, 1528
XceI RCATGY 3 cut(s) 178, 441, 781
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 3 cut(s) 309, 1394, 1538
XspI CTAG 2 cut(s) 272, 744
ZrmI AGTACT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.