pycom05g22910

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
25041172 .. 25043766
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGGAAATTGATGACCATATAGGATGTGCCATGAGTGGACTGATTGCTGATGCTCGTACACTTGTTGAACATGCGCGAGTTGAAACTCAGAATCATAGGTTCTCCTATGGTGAGCCAATGACTGTGGAGTCTACAACACAAGCTCTTTGTGATCTTGCCCTACGATTTGGTGAAGGTGATGAAGAGTCCATGTCTCGACCATTTGGGGTATCTCTCCTAATTGCTGGTCATGATGAGCACGGGCCCAGCTTGTACTACACTGATCCATCTGGCACATTTTGGCAGTGCAATGCAAAAGCTATCGGTTCAGGTTCGGAGGGAGCAGACAGCTCTCTTCAAGAGCAATACAACAAGGACTTGACTCTTCTAGAAGCTGAGACAATTGCTCTGTCTATCCTTAAGCAAGTTATGGAAGAAAAGGTGACACCCAACAATGTAGATATCGCAAAGGTTTCTCCAACATATCATCTGTATACCCCCTCTGAGGTGGAGGCCGTCATTGCTCTTCGGATTTGTTTCTGGTTAACCAGTAAAAGTCATTCGGTAGTGCAGTGCAATACTAAAACATTCCGAGGATATTATCATCTTACTTTGATGAGCAGATGCCGTCCTGCTTTGTTTTGTGCACAAATCTGTGGTCAATGCAGGCTAGCAGTTGAAATACATGTTGAAGTCGGTTTCCGTTTGTGTTTCTACTGCAGGCCAGCAGTAGAATTGCACCGTGCTATTAGATCACTTCCACGCTCTATACCTGTGTTTGATTGCAGAGGGGTAATTAGATTTGGAGCTATGAAGGCTGTTTCTTTTACAAGAAAATTAATGAAAATATGCCAGGATCGATTTTTTAGTGTAAAAATGTGGTTTTTCGTTAAAATGAACAGTATCAATGGTCGCTCTAAAACTTCATACTCAAAAGTTGAACTCGCTCTGCCAGGCGTTTCAAAATATATCGACAAGTTAATTGGTCCTCTTGCACACTGTTTATTTTTAGTCCGTAGATTGAATAAATTGAAGGAAAATCAAGGACCAAAGAGAAGCATGCTGTGCACGAGAAGAAAAAATGTGTTCGTTTGTCATTACCATTCCTTAGATCAGCAGATTTTCCAAACAATAAAAAATTGCTCAACTTGTTTACACGGGAAACGAGAAAACCGATTAAGGTGTTTGGATATATGCAATGAAGACCCATTGAAATTGCTTCGAGCAGAGGAAGATCCAGGAAAACTTGGAAAGAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019773 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome Proteasome_A_N

Protein Analysis

414

Amino Acids

46.78

Weight (kDa)

8.86

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 1 - 151 1.4e-42 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 127
AccI GTMKAC 2 cut(s) 131, 473
AccII CGCG 1 cut(s) 76
AclWI GGATC 3 cut(s) 257, 843, 1208
AfaI GTAC 2 cut(s) 58, 254
AfiI CCNNNNNNNGG 1 cut(s) 484
AflII CTTAAG 1 cut(s) 398
AflIII ACRYGT 1 cut(s) 664
AjnI CCWGG 3 cut(s) 831, 931, 1216
AluBI AGCT 7 cut(s) 143, 249, 299, 330, 374, 788, 1237
AluI AGCT 7 cut(s) 143, 249, 299, 330, 374, 788, 1237
Alw21I GWGCWC 3 cut(s) 240, 628, 1049
Alw26I GTCTC 2 cut(s) 198, 371
Alw44I GTGCAC 2 cut(s) 624, 1045
AlwI GGATC 3 cut(s) 257, 843, 1208
AoxI GGCC 3 cut(s) 242, 492, 701
ApaI GGGCCC 1 cut(s) 246
ApaLI GTGCAC 2 cut(s) 624, 1045
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
AseI ATTAAT 1 cut(s) 818
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 4 cut(s) 242, 243, 965, 1025
AsuHPI GGTGA 4 cut(s) 122, 182, 188, 433
AsuNHI GCTAGC 1 cut(s) 649
AvaII GGWCC 2 cut(s) 965, 1025
BaeGI GKGCMC 3 cut(s) 246, 628, 1049
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 1048
BbsI GAAGAC 1 cut(s) 1188
Bbv12I GWGCWC 3 cut(s) 240, 628, 1049
BccI CCATC 1 cut(s) 274
BceAI ACGGC 2 cut(s) 479, 591
BciT130I CCWGG 3 cut(s) 833, 933, 1218
BcoDI GTCTC 2 cut(s) 198, 371
BfaI CTAG 2 cut(s) 368, 650
BfmI CTRYAG 1 cut(s) 697
BfrI CTTAAG 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 833, 933, 1218
Bme18I GGWCC 2 cut(s) 965, 1025
BmgT120I GGNCC 4 cut(s) 242, 243, 965, 1025
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 3 cut(s) 833, 933, 1218
BmsI GCATC 2 cut(s) 40, 593
BmtI GCTAGC 1 cut(s) 653
BpiI GAAGAC 1 cut(s) 1188
Bsa29I ATCGAT 1 cut(s) 838
BsaJI CCNNGG 1 cut(s) 571
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 295, 498, 1183
BseBI CCWGG 3 cut(s) 833, 933, 1218
BseCI ATCGAT 1 cut(s) 838
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 3 cut(s) 295, 498, 1183
BseMII CTCAG 3 cut(s) 101, 366, 474
BseNI ACTGG 1 cut(s) 528
BseSI GKGCMC 3 cut(s) 246, 628, 1049
BseYI CCCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 569
Bsh1236I CGCG 1 cut(s) 76
BshFI GGCC 3 cut(s) 244, 494, 703
BshVI ATCGAT 1 cut(s) 838
BsiHKAI GWGCWC 3 cut(s) 240, 628, 1049
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 2 cut(s) 198, 371
BsnI GGCC 3 cut(s) 244, 494, 703
Bsp120I GGGCCC 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 240, 246, 628, 1049
Bsp143I GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
BspANI GGCC 3 cut(s) 244, 494, 703
BspCNI CTCAG 3 cut(s) 100, 367, 475
BspDI ATCGAT 1 cut(s) 838
BspFNI CGCG 1 cut(s) 76
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 701
BspOI GCTAGC 1 cut(s) 653
BspPI GGATC 3 cut(s) 257, 843, 1208
BspQI GCTCTTC 1 cut(s) 510
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 3 cut(s) 295, 498, 1183
BsrI ACTGG 1 cut(s) 528
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
BssNAI GTATAC 1 cut(s) 474
BssSI CACGAG 1 cut(s) 1048
Bst1107I GTATAC 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 1048
Bst2UI CCWGG 3 cut(s) 833, 933, 1218
Bst4CI ACNGT 4 cut(s) 124, 722, 881, 980
Bst6I CTCTTC 4 cut(s) 177, 339, 369, 510
BstAFI CTTAAG 1 cut(s) 398
BstAPI GCANNNNNTGC 1 cut(s) 1044
BstC8I GCNNGC 5 cut(s) 647, 651, 701, 705, 1040
BstDEI CTNAG 4 cut(s) 87, 375, 483, 1087
BstF5I GGATG 1 cut(s) 29
BstFNI CGCG 1 cut(s) 76
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 6 cut(s) 154, 265, 734, 838, 1093, 1216
BstMAI GTCTC 2 cut(s) 198, 371
BstMBI GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
BstMWI GCNNNNNNNGC 3 cut(s) 500, 794, 1044
BstNI CCWGG 3 cut(s) 833, 933, 1218
BstNSI RCATGY 3 cut(s) 74, 668, 1042
BstSCI CCNGG 3 cut(s) 831, 931, 1216
BstSFI CTRYAG 1 cut(s) 697
BstSLI GKGCMC 3 cut(s) 246, 628, 1049
BstUI CGCG 1 cut(s) 76
BstV2I GAAGAC 1 cut(s) 1188
BstX2I RGATCY 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 1213
BstZ17I GTATAC 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 838
BsuRI GGCC 3 cut(s) 244, 494, 703
BsuTUI ATCGAT 1 cut(s) 838
BtsCI GGATG 1 cut(s) 29
BtsI GCAGTG 2 cut(s) 290, 557
BtsIMutI CAGTG 4 cut(s) 258, 290, 557, 976
Cac8I GCNNGC 5 cut(s) 647, 651, 701, 705, 1040
CciI TCATGA 1 cut(s) 229
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 4 cut(s) 242, 243, 965, 1025
ClaI ATCGAT 1 cut(s) 838
Csp6I GTAC 2 cut(s) 57, 253
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 6 cut(s) 31, 71, 190, 230, 665, 1039
CviQI GTAC 2 cut(s) 57, 253
DdeI CTNAG 4 cut(s) 87, 375, 483, 1087
DpnI GATC 6 cut(s) 153, 264, 733, 837, 1092, 1215
DpnII GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
DrdI GACNNNNNNGTC 1 cut(s) 127
DseDI GACNNNNNNGTC 1 cut(s) 127
Eam1104I CTCTTC 4 cut(s) 177, 339, 369, 510
EarI CTCTTC 4 cut(s) 177, 339, 369, 510
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 965, 1025
EcoRII CCWGG 3 cut(s) 831, 931, 1216
EcoRV GATATC 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 6 cut(s) 34, 74, 193, 233, 668, 1042
FatI CATG 6 cut(s) 30, 70, 189, 229, 664, 1038
FblI GTMKAC 2 cut(s) 131, 473
FokI GGATG 1 cut(s) 36
FriOI GRGCYC 1 cut(s) 246
FspBI CTAG 2 cut(s) 368, 650
GlaI GCGC 1 cut(s) 75
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 3 cut(s) 244, 494, 703
HhaI GCGC 1 cut(s) 76
Hin1II CATG 6 cut(s) 34, 74, 193, 233, 668, 1042
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 1235
HinfI GANTC 4 cut(s) 91, 128, 185, 361
HpaI GTTAAC 1 cut(s) 525
HphI GGTGA 4 cut(s) 122, 182, 188, 433
Hpy166II GTNNAC 8 cut(s) 38, 59, 132, 474, 525, 626, 1047, 1133
Hpy188I TCNGA 5 cut(s) 90, 316, 484, 510, 572
Hpy188III TCNNGA 4 cut(s) 195, 230, 338, 368
Hpy8I GTNNAC 8 cut(s) 38, 59, 132, 474, 525, 626, 1047, 1133
HpyAV CCTTC 3 cut(s) 167, 787, 1006
HpyCH4III ACNGT 4 cut(s) 124, 722, 881, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 794, 1044
HpyF3I CTNAG 4 cut(s) 87, 375, 483, 1087
Hsp92II CATG 6 cut(s) 34, 74, 193, 233, 668, 1042
HspAI GCGC 1 cut(s) 74
KspAI GTTAAC 1 cut(s) 525
Kzo9I GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 2 cut(s) 320, 785
LweI GCATC 2 cut(s) 40, 593
MaeI CTAG 2 cut(s) 368, 650
MaeIII GTNAC 1 cut(s) 421
MalI GATC 6 cut(s) 153, 264, 733, 837, 1092, 1215
MboI GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
MboII GAAGA 8 cut(s) 194, 326, 356, 425, 497, 1065, 1193, 1223
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 4 cut(s) 240, 246, 628, 1049
MlyI GAGTC 3 cut(s) 137, 194, 355
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 8 cut(s) 310, 478, 484, 490, 566, 761, 978, 1201
MseI TTAA 6 cut(s) 399, 524, 818, 870, 959, 1157
MspCI CTTAAG 1 cut(s) 398
MspR9I CCNGG 3 cut(s) 833, 933, 1218
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 3 cut(s) 833, 933, 1218
MvnI CGCG 1 cut(s) 76
MwoI GCNNNNNNNGC 3 cut(s) 500, 794, 1044
NdeII GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
NheI GCTAGC 1 cut(s) 649
NlaIII CATG 6 cut(s) 34, 74, 193, 233, 668, 1042
NlaIV GGNNCC 1 cut(s) 244
NmuCI GTSAC 1 cut(s) 421
NspI RCATGY 3 cut(s) 74, 668, 1042
PaeI GCATGC 1 cut(s) 1042
PagI TCATGA 1 cut(s) 229
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 510
PcsI WCGNNNNNNNCGW 1 cut(s) 1150
PfeI GAWTC 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 1216
PleI GAGTC 3 cut(s) 136, 193, 355
PpsI GAGTC 3 cut(s) 136, 193, 355
PscI ACATGT 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 818
Psp6I CCWGG 3 cut(s) 831, 931, 1216
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 3 cut(s) 831, 931, 1216
PspN4I GGNNCC 1 cut(s) 244
PspOMI GGGCCC 1 cut(s) 242
PspPI GGNCC 4 cut(s) 242, 243, 965, 1025
PstI CTGCAG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 1213
RsaI GTAC 2 cut(s) 58, 254
RsaNI GTAC 2 cut(s) 57, 253
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 6 cut(s) 399, 524, 818, 870, 959, 1157
Sau3AI GATC 6 cut(s) 151, 262, 731, 835, 1090, 1213
Sau96I GGNCC 4 cut(s) 242, 243, 965, 1025
SchI GAGTC 3 cut(s) 137, 194, 355
ScrFI CCNGG 3 cut(s) 833, 933, 1218
SduI GDGCHC 4 cut(s) 240, 246, 628, 1049
SfaNI GCATC 2 cut(s) 40, 593
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 2 cut(s) 965, 1025
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SphI GCATGC 1 cut(s) 1042
SspMI CTAG 2 cut(s) 368, 650
StyD4I CCNGG 3 cut(s) 831, 931, 1216
TaaI ACNGT 4 cut(s) 124, 722, 881, 980
TaqI TCGA 4 cut(s) 196, 838, 951, 1201
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 91
Tru1I TTAA 6 cut(s) 399, 524, 818, 870, 959, 1157
Tru9I TTAA 6 cut(s) 399, 524, 818, 870, 959, 1157
TscAI CASTG 4 cut(s) 265, 290, 557, 983
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 6 cut(s) 195, 806, 836, 890, 894, 1194
TspGWI ACGGA 2 cut(s) 671, 983
TspRI CASTG 4 cut(s) 265, 290, 557, 983
Vha464I CTTAAG 1 cut(s) 398
VneI GTGCAC 2 cut(s) 624, 1045
VpaK11BI GGWCC 2 cut(s) 965, 1025
VspI ATTAAT 1 cut(s) 818
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 3 cut(s) 74, 668, 1042
XmiI GTMKAC 2 cut(s) 131, 473
XspI CTAG 2 cut(s) 368, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.