pycom05g24120

Root hair defective 3 GTP-binding protein (RHD3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
25691201 .. 25691602
402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 291 bp
ATGGATGCTGCATTGAAGGCAGCCACCACATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAAAAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATACTGTTGGGAACAGCACGAGGACTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCAAGTAATATTTTGCTCGATGCAGAACTCTCCCCAAAAATATCAGATTTTGGATTGGCAAAGCTTTATGATGACGAGAAAACCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

97

Amino Acids

11.32

Weight (kDa)

8.52

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 33
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 33, 69, 162
AgsI TTSAA 3 cut(s) 16, 52, 120
AluBI AGCT 1 cut(s) 264
AluI AGCT 1 cut(s) 264
AoxI GGCC 2 cut(s) 106, 172
ApeKI GCWGC 2 cut(s) 8, 20
BauI CACGAG 1 cut(s) 140
BbvI GCAGC 1 cut(s) 32
BclI TGATCA 1 cut(s) 66
BisI GCNGC 2 cut(s) 9, 21
BlsI GCNGC 2 cut(s) 10, 22
BmsI GCATC 1 cut(s) 210
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 69, 162
Bse1I ACTGG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 33, 69, 162
BseNI ACTGG 1 cut(s) 109
BseRI GAGGAG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 32
BshFI GGCC 2 cut(s) 108, 174
BslI CCNNNNNNNGG 3 cut(s) 33, 69, 162
BsnI GGCC 2 cut(s) 108, 174
Bsp1407I TGTACA 1 cut(s) 183
Bsp143I GATC 1 cut(s) 66
BspANI GGCC 2 cut(s) 108, 174
BspHI TCATGA 1 cut(s) 159
BsrGI TGTACA 1 cut(s) 183
BsrI ACTGG 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 140
BssT1I CCWWGG 1 cut(s) 175
Bst2BI CACGAG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 128
BstAUI TGTACA 1 cut(s) 183
BstENI CCTNNNNNAGG 2 cut(s) 67, 160
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 1 cut(s) 69
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 32
BsuRI GGCC 2 cut(s) 108, 174
BtsCI GGATG 1 cut(s) 10
CciI TCATGA 1 cut(s) 159
Csp6I GTAC 1 cut(s) 184
CviAII CATG 2 cut(s) 160, 288
CviJI RGCY 5 cut(s) 23, 62, 108, 174, 264
CviKI_1 RGCY 5 cut(s) 23, 62, 108, 174, 264
CviQI GTAC 1 cut(s) 184
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eco130I CCWWGG 1 cut(s) 175
EcoNI CCTNNNNNAGG 2 cut(s) 67, 160
EcoT14I CCWWGG 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 2 cut(s) 163, 291
FaiI YATR 5 cut(s) 41, 124, 161, 269, 289
FatI CATG 2 cut(s) 159, 287
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 9, 21
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 9, 21
GluI GCNGC 2 cut(s) 9, 21
HaeIII GGCC 2 cut(s) 108, 174
Hin1II CATG 2 cut(s) 163, 291
HindIII AAGCTT 1 cut(s) 262
HinfI GANTC 2 cut(s) 116, 166
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 3 cut(s) 49, 160, 190
HpyAV CCTTC 2 cut(s) 10, 166
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 3 cut(s) 11, 96, 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 163, 291
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 1 cut(s) 66
LpnPI CCDG 2 cut(s) 55, 90
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 88
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 175
MnlI CCTC 2 cut(s) 136, 156
MslI CAYNNNNRTG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 1 cut(s) 66
NlaIII CATG 2 cut(s) 163, 291
PagI TCATGA 1 cut(s) 159
PfeI GAWTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 10, 22
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 191
SatI GCNGC 2 cut(s) 9, 21
Sau3AI GATC 1 cut(s) 66
SchI GAGTC 1 cut(s) 175
SetI ASST 3 cut(s) 101, 158, 266
SfaNI GCATC 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 191
SspI AATATT 1 cut(s) 210
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 1 cut(s) 128
TaqI TCGA 3 cut(s) 114, 189, 218
TatI WGTACW 1 cut(s) 183
TfiI GAWTC 1 cut(s) 116
TseI GCWGC 2 cut(s) 8, 20
TspDTI ATGAA 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 33
XagI CCTNNNNNAGG 2 cut(s) 67, 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.