pycom05g25030

Acyl-coenzyme A thioesterase 9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
26471436 .. 26471747
312 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 312 bp
ATGCGTTGTGGGAAGCGAGGTCGAAGATCTTCGAGAATCGAGAGGTTCTTTATGGACCCGCCCAAAGACGCGCCGCCGCAGAGTGAGTTGCTGAAGAAAACGCCGTCACATAACAGGACGAAGATTCTCTACAATGCATTTGTTGGTGTTTCGAAAACGTCGTTGAAGGCGAGGAGGGAACCTCGGCAAACTCCAGTTGGTGTTGCGGGAAATTCCTCTGATTCGGGCCGGGTTGGGTCGTTTGTTGTACCTTCTGCATGCTCAAGTCTGCAAACTCCAGTTGGTGTTGCAGATTCGGCAGATCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

104

Amino Acids

11.15

Weight (kDa)

10.66

Isoelectric Point (pI)

70.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AciI CCGC 4 cut(s) 59, 74, 77, 206
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 249
AgsI TTSAA 1 cut(s) 166
AoxI GGCC 1 cut(s) 226
ApoI RAATTY 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 55, 226
AsuC2I CCSGG 1 cut(s) 230
AsuII TTCGAA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BceAI ACGGC 1 cut(s) 88
BcnI CCSGG 1 cut(s) 230
BglII AGATCT 1 cut(s) 26
BisI GCNGC 2 cut(s) 74, 77
BlsI GCNGC 2 cut(s) 75, 78
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 2 cut(s) 55, 226
BmiI GGNNCC 2 cut(s) 57, 180
BmrFI CCNGG 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 166, 198
BpmI CTGGAG 2 cut(s) 177, 261
Bpu14I TTCGAA 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 194, 278
BseDI CCNNGG 1 cut(s) 182
BseNI ACTGG 2 cut(s) 194, 278
BseRI GAGGAG 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 228
BsiSI CCGG 1 cut(s) 229
BsnI GGCC 1 cut(s) 228
Bsp119I TTCGAA 1 cut(s) 152
Bsp143I GATC 2 cut(s) 26, 301
BspACI CCGC 4 cut(s) 59, 74, 77, 206
BspANI GGCC 1 cut(s) 228
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 57, 180
BspT104I TTCGAA 1 cut(s) 152
BsrI ACTGG 2 cut(s) 194, 278
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 2 cut(s) 26, 301
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 259
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 2 cut(s) 29, 304
BstMBI GATC 2 cut(s) 26, 301
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 228
BstUI CGCG 1 cut(s) 71
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BsuRI GGCC 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 259
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 55, 226
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 248
CviAII CATG 1 cut(s) 258
CviJI RGCY 1 cut(s) 228
CviKI_1 RGCY 1 cut(s) 228
CviQI GTAC 1 cut(s) 248
DpnI GATC 2 cut(s) 28, 303
DpnII GATC 2 cut(s) 26, 301
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 139
FaeI CATG 1 cut(s) 261
FaiI YATR 3 cut(s) 53, 111, 259
FatI CATG 1 cut(s) 257
FauI CCCGC 2 cut(s) 66, 199
Fnu4HI GCNGC 2 cut(s) 74, 77
Fsp4HI GCNGC 2 cut(s) 74, 77
GlaI GCGC 1 cut(s) 72
GluI GCNGC 2 cut(s) 74, 77
GsuI CTGGAG 2 cut(s) 177, 261
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 229
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 73
Hin1II CATG 1 cut(s) 261
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 4 cut(s) 36, 124, 221, 293
HpaII CCGG 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 220, 306
Hpy188III TCNNGA 2 cut(s) 33, 40
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 160, 261
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 4 cut(s) 137, 257, 271, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 1 cut(s) 261
HspAI GCGC 1 cut(s) 71
Kzo9I GATC 2 cut(s) 26, 301
LpnPI CCDG 4 cut(s) 100, 207, 242, 291
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 105
MalI GATC 2 cut(s) 28, 303
MboI GATC 2 cut(s) 26, 301
MboII GAAGA 4 cut(s) 21, 36, 106, 133
MflI RGATCY 1 cut(s) 26
MluCI AATT 1 cut(s) 211
MnlI CCTC 6 cut(s) 11, 36, 165, 168, 192, 226
Mph1103I ATGCAT 1 cut(s) 139
MroXI GAANNNNTTC 1 cut(s) 28
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 230
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 230
NdeII GATC 2 cut(s) 26, 301
NlaIII CATG 1 cut(s) 261
NlaIV GGNNCC 2 cut(s) 57, 180
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 105
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 1 cut(s) 261
NspV TTCGAA 1 cut(s) 152
PaeI GCATGC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 167
PdmI GAANNNNTTC 1 cut(s) 28
PfeI GAWTC 4 cut(s) 36, 124, 221, 293
PkrI GCNGC 2 cut(s) 75, 78
PspN4I GGNNCC 2 cut(s) 57, 180
PspPI GGNCC 2 cut(s) 55, 226
PsuI RGATCY 1 cut(s) 26
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
SatI GCNGC 2 cut(s) 74, 77
Sau3AI GATC 2 cut(s) 26, 301
Sau96I GGNCC 2 cut(s) 55, 226
ScrFI CCNGG 1 cut(s) 230
SetI ASST 6 cut(s) 22, 47, 161, 184, 253, 311
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
SphI GCATGC 1 cut(s) 261
Sse9I AATT 1 cut(s) 211
SsiI CCGC 4 cut(s) 59, 74, 77, 206
StyD4I CCNGG 1 cut(s) 228
TaiI ACGT 1 cut(s) 161
TaqI TCGA 4 cut(s) 22, 32, 39, 152
TasI AATT 1 cut(s) 211
TauI GCSGC 2 cut(s) 76, 79
TfiI GAWTC 4 cut(s) 36, 124, 221, 293
TseFI GTSAC 1 cut(s) 105
Tsp45I GTSAC 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.