pycom05g25240

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
26691354 .. 26691725
372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 372 bp
ATGGTGCGTATCGATTATGCCACATGGGGTATTAACCCTCCACACACGCATCCTCGCGCTACAGAAATCTTGACAGTCCTGGACGGGAGCCTCCAAGTGGGTATTATCACTTCCAACCCGGAAAACCGTCATATCAGCAAGGTGCTTCAGAAGGGTGATGTGTTTGTGTTCCCAGTAGGACTTGTTCATCACCAACACAATGTTGGAAATAGTAATGCAGTAGTCCTTGCTGCTCTCAGCAGCCAAAACCCTGGTGCCATTACCGTTGCAAATACAATATTTGGATCAAAGCCTGATATCCCTGCTGATGTTCTTGCAAAGTCTTTCCAAGTGGACAGCAATGTAGTCTTCGATTTGCAGGCAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

124

Amino Acids

13.23

Weight (kDa)

6.57

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 1 - 115 1.1e-38 Cupin
Cupin_2 PF07883 11 - 77 2e-09 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 254
AccII CGCG 1 cut(s) 57
AclWI GGATC 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 97
AjnI CCWGG 2 cut(s) 78, 250
AlwI GGATC 1 cut(s) 292
ApeKI GCWGC 2 cut(s) 230, 240
AspLEI GCGC 1 cut(s) 59
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 167, 182
BanI GGYRCC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 94, 126
BbsI GAAGAC 1 cut(s) 340
BbvI GCAGC 2 cut(s) 217, 252
BciT130I CCWGG 2 cut(s) 80, 252
BcnI CCSGG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 2 cut(s) 231, 241
BlsI GCNGC 2 cut(s) 232, 242
Bme1390I CCNGG 3 cut(s) 80, 119, 252
BmiI GGNNCC 2 cut(s) 89, 256
BmrFI CCNGG 3 cut(s) 80, 119, 252
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 58
BmuI ACTGGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 340
BpuMI CCSGG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 346
BseBI CCWGG 2 cut(s) 80, 252
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 217, 252
Bsh1236I CGCG 1 cut(s) 57
BshNI GGYRCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 12
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 97
Bsp143I GATC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 12
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 89, 256
BspPI GGATC 1 cut(s) 292
BspT107I GGYRCC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 1 cut(s) 284
Bst2UI CCWGG 2 cut(s) 80, 252
Bst4CI ACNGT 3 cut(s) 76, 128, 265
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 1 cut(s) 236
BstF5I GGATG 1 cut(s) 49
BstFNI CGCG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 59
BstKTI GATC 1 cut(s) 287
BstMBI GATC 1 cut(s) 284
BstNI CCWGG 2 cut(s) 80, 252
BstSCI CCNGG 3 cut(s) 78, 117, 250
BstSFI CTRYAG 1 cut(s) 60
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 2 cut(s) 217, 252
BstV2I GAAGAC 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 12
BsuTUI ATCGAT 1 cut(s) 12
BtsCI GGATG 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 59
ClaI ATCGAT 1 cut(s) 12
CviAII CATG 1 cut(s) 24
CviJI RGCY 3 cut(s) 90, 243, 292
CviKI_1 RGCY 3 cut(s) 90, 243, 292
DdeI CTNAG 1 cut(s) 236
DpnI GATC 1 cut(s) 286
DpnII GATC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 78, 250
EcoRV GATATC 1 cut(s) 298
FaeI CATG 1 cut(s) 27
FaiI YATR 3 cut(s) 18, 25, 132
FatI CATG 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 231, 241
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 2 cut(s) 231, 241
GlaI GCGC 1 cut(s) 58
GluI GCNGC 2 cut(s) 231, 241
HapII CCGG 1 cut(s) 119
HhaI GCGC 1 cut(s) 59
Hin1II CATG 1 cut(s) 27
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HpaII CCGG 1 cut(s) 119
HphI GGTGA 2 cut(s) 167, 182
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 150
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 3 cut(s) 76, 128, 265
HpyCH4V TGCA 4 cut(s) 218, 269, 317, 358
HpyF3I CTNAG 1 cut(s) 236
Hsp92II CATG 1 cut(s) 27
HspAI GCGC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 284
LmnI GCTCC 1 cut(s) 87
LpnPI CCDG 9 cut(s) 65, 92, 132, 186, 237, 264, 306, 315, 344
Lsp1109I GCAGC 2 cut(s) 217, 252
LweI GCATC 1 cut(s) 58
MalI GATC 1 cut(s) 286
MboI GATC 1 cut(s) 284
MboII GAAGA 1 cut(s) 340
MmeI TCCRAC 2 cut(s) 138, 184
MnlI CCTC 3 cut(s) 48, 63, 101
MseI TTAA 1 cut(s) 33
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 3 cut(s) 80, 119, 252
MvaI CCWGG 2 cut(s) 80, 252
MvnI CGCG 1 cut(s) 57
NciI CCSGG 1 cut(s) 119
NdeII GATC 1 cut(s) 284
NlaIII CATG 1 cut(s) 27
NlaIV GGNNCC 2 cut(s) 89, 256
PfoI TCCNGGA 1 cut(s) 78
PkrI GCNGC 2 cut(s) 232, 242
Psp6I CCWGG 2 cut(s) 78, 250
PspGI CCWGG 2 cut(s) 78, 250
PspN4I GGNNCC 2 cut(s) 89, 256
PsrI GAACNNNNNNTAC 2 cut(s) 168, 200
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 2 cut(s) 231, 241
Sau3AI GATC 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 80, 119, 252
SetI ASST 1 cut(s) 144
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 1 cut(s) 60
SspI AATATT 1 cut(s) 279
StyD4I CCNGG 3 cut(s) 78, 117, 250
TaaI ACNGT 3 cut(s) 76, 128, 265
TaqI TCGA 2 cut(s) 12, 351
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TseI GCWGC 2 cut(s) 230, 240
TspDTI ATGAA 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.