pycom05g26470

urease accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
27737925 .. 27739612
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGCTGACCACCACCATGATCATCATGACCATGACGATCACCACCATCATGATCATGATCATGGTCATAGCCATGGGAGTTCAGCAGCACCAAATTCATTTGTGGGTCCAGATGGGAAGGTGTACCATAGCCACGATGGGCTGGCACCACACTCACACGAACCCATATACTCCCCCGGGTACTTCAGCAGAAGAGCTCCACCTCTCCTCTCCAGGAATTTCAATGAAAGGGCTTTCACCATTGGCATTGGTGGACCTGTTGGTACTGGGAAAACTGCTTTGATGTTGGCTCTGTGTAAACTTTTGCGTGACAAGTACAGTCTTGCTGCTGTGACAAATGATATATTCACAAAAGAGGATGGTGAGTTCTTGGTGAAGAATGGAGCACTTCCGGAAGAAAGAATACGTGCTGTGGAAACTGGGGGTTGCCCACATGCTGCAATTCGCGAAGACATCAGTATTAACCTTGGCCCTCTTGAGGAGCTTTCAAACTTGTACAAGACCGACATACTGCTTTGTGAATCTGGCGGAGATAATTTAGCCGCATACTTCAGCCGGGAGCTGGCTGACTATATCATTTACATAATCGATGTATCTGGTGGTGATAAAATTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006807 GO:0008150 GO:0008152 GO:0043085 GO:0044093 GO:0050790 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
cobW

Protein Analysis

206

Amino Acids

22.45

Weight (kDa)

5.74

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cobW PF02492 80 - 199 2.3e-22 CobW/HypB/UreG, nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AccII CGCG 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 2 cut(s) 528, 543
AcsI RAATTY 3 cut(s) 94, 217, 609
AcuI CTGAAG 2 cut(s) 169, 535
AfaI GTAC 5 cut(s) 125, 182, 265, 317, 497
AfiI CCNNNNNNNGG 2 cut(s) 478, 562
AgsI TTSAA 2 cut(s) 223, 489
AjnI CCWGG 1 cut(s) 212
AloI GAACNNNNNNTCC 2 cut(s) 350, 382
AluBI AGCT 3 cut(s) 197, 484, 562
AluI AGCT 3 cut(s) 197, 484, 562
Alw21I GWGCWC 2 cut(s) 199, 388
Ama87I CYCGRG 1 cut(s) 176
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 3 cut(s) 86, 326, 437
ApoI RAATTY 3 cut(s) 94, 217, 609
AspS9I GGNCC 3 cut(s) 107, 254, 470
AsuC2I CCSGG 3 cut(s) 177, 178, 557
AsuHPI GGTGA 5 cut(s) 32, 229, 374, 385, 614
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 107, 254
BanI GGYRCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 387, 419
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 2 cut(s) 199, 388
BbvI GCAGC 3 cut(s) 98, 313, 424
BccI CCATC 4 cut(s) 54, 107, 131, 353
BciT130I CCWGG 1 cut(s) 214
BclI TGATCA 3 cut(s) 19, 52, 58
BcnI CCSGG 3 cut(s) 177, 178, 557
BisI GCNGC 4 cut(s) 87, 327, 438, 543
BlsI GCNGC 4 cut(s) 88, 328, 439, 544
Bme1390I CCNGG 4 cut(s) 177, 178, 214, 557
Bme18I GGWCC 2 cut(s) 107, 254
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 3 cut(s) 107, 254, 470
BmiI GGNNCC 2 cut(s) 108, 147
BmrFI CCNGG 4 cut(s) 177, 178, 214, 557
BmrI ACTGGG 2 cut(s) 276, 429
BmuI ACTGGG 2 cut(s) 276, 429
BpiI GAAGAC 1 cut(s) 456
BpmI CTGGAG 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 3 cut(s) 177, 178, 557
Bsa29I ATCGAT 1 cut(s) 588
BsaAI YACGTR 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 57
BsaJI CCNNGG 4 cut(s) 73, 175, 176, 466
BsaWI WCCGGW 1 cut(s) 391
Bsc4I CCNNNNNNNGG 2 cut(s) 478, 562
Bse1I ACTGG 2 cut(s) 271, 424
Bse8I GATNNNNATC 1 cut(s) 57
BseAI TCCGGA 1 cut(s) 391
BseBI CCWGG 1 cut(s) 214
BseCI ATCGAT 1 cut(s) 588
BseDI CCNNGG 4 cut(s) 73, 175, 176, 466
BseGI GGATG 1 cut(s) 364
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 2 cut(s) 478, 562
BseNI ACTGG 2 cut(s) 271, 424
BseRI GAGGAG 2 cut(s) 197, 494
BseXI GCAGC 3 cut(s) 98, 313, 424
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 1 cut(s) 471
BshNI GGYRCC 1 cut(s) 145
BshVI ATCGAT 1 cut(s) 588
BsiHKAI GWGCWC 2 cut(s) 199, 388
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 3 cut(s) 177, 392, 556
BslI CCNNNNNNNGG 2 cut(s) 478, 562
BsnI GGCC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 2 cut(s) 199, 388
Bsp13I TCCGGA 1 cut(s) 391
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 4 cut(s) 19, 37, 52, 58
Bsp19I CCATGG 1 cut(s) 73
Bsp68I TCGCGA 1 cut(s) 447
BspACI CCGC 2 cut(s) 528, 543
BspANI GGCC 1 cut(s) 471
BspDI ATCGAT 1 cut(s) 588
BspEI TCCGGA 1 cut(s) 391
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 3 cut(s) 25, 49, 55
BspLI GGNNCC 2 cut(s) 108, 147
BspQI GCTCTTC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 2 cut(s) 271, 424
BssECI CCNNGG 4 cut(s) 73, 175, 176, 466
BssMI GATC 4 cut(s) 19, 37, 52, 58
BssT1I CCWWGG 2 cut(s) 73, 466
Bst2UI CCWGG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 495
BstBAI YACGTR 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 144, 564
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 447
BstKTI GATC 4 cut(s) 22, 40, 55, 61
BstMBI GATC 4 cut(s) 19, 37, 52, 58
BstNI CCWGG 1 cut(s) 214
BstNSI RCATGY 1 cut(s) 437
BstSCI CCNGG 4 cut(s) 175, 176, 212, 555
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 98, 313, 424
BstV2I GAAGAC 1 cut(s) 456
Bsu15I ATCGAT 1 cut(s) 588
BsuRI GGCC 1 cut(s) 471
BsuTUI ATCGAT 1 cut(s) 588
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 1 cut(s) 364
BtuMI TCGCGA 1 cut(s) 447
Cac8I GCNNGC 2 cut(s) 144, 564
CciI TCATGA 3 cut(s) 25, 49, 55
Cfr13I GGNCC 3 cut(s) 107, 254, 470
Cfr9I CCCGGG 1 cut(s) 176
ClaI ATCGAT 1 cut(s) 588
Csp6I GTAC 5 cut(s) 124, 181, 264, 316, 496
CviAII CATG 9 cut(s) 17, 26, 32, 50, 56, 62, 74, 434, 615
CviQI GTAC 5 cut(s) 124, 181, 264, 316, 496
DpnI GATC 4 cut(s) 21, 39, 54, 60
DpnII GATC 4 cut(s) 19, 37, 52, 58
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
EciI GGCGGA 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 2 cut(s) 73, 466
Eco24I GRGCYC 1 cut(s) 199
Eco47I GGWCC 2 cut(s) 107, 254
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 2 cut(s) 169, 535
Eco88I CYCGRG 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 73, 466
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 73, 466
FaeI CATG 9 cut(s) 20, 29, 35, 53, 59, 65, 77, 437, 618
FatI CATG 9 cut(s) 16, 25, 31, 49, 55, 61, 73, 433, 614
FbaI TGATCA 3 cut(s) 19, 52, 58
Fnu4HI GCNGC 4 cut(s) 87, 327, 438, 543
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 4 cut(s) 87, 327, 438, 543
GluI GCNGC 4 cut(s) 87, 327, 438, 543
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 471
HapII CCGG 3 cut(s) 177, 392, 556
Hin1II CATG 9 cut(s) 20, 29, 35, 53, 59, 65, 77, 437, 618
HinfI GANTC 1 cut(s) 521
HpaII CCGG 3 cut(s) 177, 392, 556
HphI GGTGA 5 cut(s) 32, 229, 374, 385, 614
Hpy166II GTNNAC 3 cut(s) 124, 254, 299
Hpy188III TCNNGA 7 cut(s) 26, 50, 56, 110, 392, 446, 476
Hpy8I GTNNAC 3 cut(s) 124, 254, 299
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 440
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 9 cut(s) 20, 29, 35, 53, 59, 65, 77, 437, 618
Kpn2I TCCGGA 1 cut(s) 391
Ksp22I TGATCA 3 cut(s) 19, 52, 58
Kzo9I GATC 4 cut(s) 19, 37, 52, 58
LguI GCTCTTC 1 cut(s) 187
LmnI GCTCC 4 cut(s) 202, 383, 481, 559
Lsp1109I GCAGC 3 cut(s) 98, 313, 424
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 2 cut(s) 308, 331
MalI GATC 4 cut(s) 21, 39, 54, 60
MboI GATC 4 cut(s) 19, 37, 52, 58
MboII GAAGA 4 cut(s) 204, 388, 407, 461
MhlI GDGCHC 2 cut(s) 199, 388
MluCI AATT 5 cut(s) 94, 217, 441, 535, 609
MnlI CCTC 5 cut(s) 213, 218, 349, 472, 483
MroI TCCGGA 1 cut(s) 391
MseI TTAA 1 cut(s) 462
MslI CAYNNNNRTG 6 cut(s) 15, 30, 48, 54, 60, 72
MspI CCGG 3 cut(s) 177, 392, 556
MspR9I CCNGG 4 cut(s) 177, 178, 214, 557
MvaI CCWGG 1 cut(s) 214
MvnI CGCG 1 cut(s) 447
NciI CCSGG 3 cut(s) 177, 178, 557
NcoI CCATGG 1 cut(s) 73
NdeII GATC 4 cut(s) 19, 37, 52, 58
NlaIII CATG 9 cut(s) 20, 29, 35, 53, 59, 65, 77, 437, 618
NlaIV GGNNCC 2 cut(s) 108, 147
NmuCI GTSAC 2 cut(s) 308, 331
NruI TCGCGA 1 cut(s) 447
NspI RCATGY 1 cut(s) 437
PagI TCATGA 3 cut(s) 25, 49, 55
PciSI GCTCTTC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 521
PfoI TCCNGGA 1 cut(s) 212
PkrI GCNGC 4 cut(s) 88, 328, 439, 544
Ppu21I YACGTR 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 1 cut(s) 212
PspGI CCWGG 1 cut(s) 212
PspN4I GGNNCC 2 cut(s) 108, 147
PspPI GGNCC 3 cut(s) 107, 254, 470
RruI TCGCGA 1 cut(s) 447
RsaI GTAC 5 cut(s) 125, 182, 265, 317, 497
RsaNI GTAC 5 cut(s) 124, 181, 264, 316, 496
RseI CAYNNNNRTG 6 cut(s) 15, 30, 48, 54, 60, 72
SacI GAGCTC 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 187
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 4 cut(s) 87, 327, 438, 543
Sau3AI GATC 4 cut(s) 19, 37, 52, 58
Sau96I GGNCC 3 cut(s) 107, 254, 470
ScrFI CCNGG 4 cut(s) 177, 178, 214, 557
SduI GDGCHC 2 cut(s) 199, 388
SetI ASST 8 cut(s) 123, 199, 205, 259, 409, 468, 486, 564
SinI GGWCC 2 cut(s) 107, 254
SmaI CCCGGG 1 cut(s) 178
SmiMI CAYNNNNRTG 6 cut(s) 15, 30, 48, 54, 60, 72
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 5 cut(s) 94, 217, 441, 535, 609
SsiI CCGC 2 cut(s) 528, 543
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 4 cut(s) 175, 176, 212, 555
StyI CCWWGG 2 cut(s) 73, 466
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 409
TaqI TCGA 1 cut(s) 588
TaqII GACCGA 1 cut(s) 518
TasI AATT 5 cut(s) 94, 217, 441, 535, 609
TatI WGTACW 2 cut(s) 315, 495
TauI GCSGC 1 cut(s) 545
TfiI GAWTC 1 cut(s) 521
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TseFI GTSAC 2 cut(s) 308, 331
TseI GCWGC 3 cut(s) 86, 326, 437
Tsp45I GTSAC 2 cut(s) 308, 331
TspDTI ATGAA 2 cut(s) 87, 240
TspMI CCCGGG 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 107, 254
XapI RAATTY 3 cut(s) 94, 217, 609
XceI RCATGY 1 cut(s) 437
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XmaI CCCGGG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.