pycom05g29200

DUF3700

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
29693874 .. 29694268
395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 255 bp
ATGGATTTCCTAGATGCAGTGGTACAGGCTGCATGTTCAAATGGTGTAACAGGCTGCATATTCGCAAGTGGAACAGGCCTTATGAGCTTTGTTCATCCAGTGCACAAGGTGAGGGTGACTAGGCGCGAAGACGACAATGGACAAGTTTGTGGAGTGATGTTTCAGATGGATCTGTGCCCGCGAATCCCCAGCATCCCGTGCACCGGTAGGGAATCAAATTGGGACGACAAGTGTTTTCTAACTGGTGAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

85

Amino Acids

9.22

Weight (kDa)

5.07

Isoelectric Point (pI)

25.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 7 - 55 3.9e-10 Aluminium induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 126, 181
AciI CCGC 1 cut(s) 179
AclWI GGATC 1 cut(s) 177
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 203
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 1 cut(s) 39
AluBI AGCT 1 cut(s) 87
AluI AGCT 1 cut(s) 87
Alw21I GWGCWC 2 cut(s) 105, 203
Alw44I GTGCAC 2 cut(s) 101, 199
AlwI GGATC 1 cut(s) 177
AoxI GGCC 1 cut(s) 76
ApaLI GTGCAC 2 cut(s) 101, 199
ApeKI GCWGC 2 cut(s) 29, 54
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 121, 127
BaeGI GKGCMC 3 cut(s) 105, 179, 203
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 2 cut(s) 105, 203
BbvI GCAGC 2 cut(s) 16, 41
BccI CCATC 1 cut(s) 160
BfaI CTAG 2 cut(s) 11, 120
BisI GCNGC 2 cut(s) 30, 55
BlsI GCNGC 2 cut(s) 31, 56
BmsI GCATC 2 cut(s) 4, 201
BpiI GAAGAC 1 cut(s) 135
BsaWI WCCGGW 1 cut(s) 203
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 98, 247
BseGI GGATG 2 cut(s) 94, 192
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 98, 247
BseSI GKGCMC 3 cut(s) 105, 179, 203
BseXI GCAGC 2 cut(s) 16, 41
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 2 cut(s) 126, 181
BshFI GGCC 1 cut(s) 78
BshTI ACCGGT 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 105, 203
BsiSI CCGG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 78
Bsp1286I GDGCHC 3 cut(s) 105, 179, 203
Bsp143I GATC 1 cut(s) 169
BspACI CCGC 1 cut(s) 179
BspANI GGCC 1 cut(s) 78
BspFNI CGCG 2 cut(s) 126, 181
BspPI GGATC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 98, 247
BssAI RCCGGY 1 cut(s) 203
BssMI GATC 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 179
BstF5I GGATG 2 cut(s) 94, 192
BstFNI CGCG 2 cut(s) 126, 181
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 1 cut(s) 172
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 84, 198
BstNSI RCATGY 1 cut(s) 36
BstSLI GKGCMC 3 cut(s) 105, 179, 203
BstUI CGCG 2 cut(s) 126, 181
BstV1I GCAGC 2 cut(s) 16, 41
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 1 cut(s) 78
BtsCI GGATG 2 cut(s) 94, 192
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 105
Cac8I GCNNGC 1 cut(s) 179
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 203
Csp6I GTAC 1 cut(s) 23
CspAI ACCGGT 1 cut(s) 203
CviAII CATG 1 cut(s) 33
CviJI RGCY 4 cut(s) 29, 54, 78, 87
CviKI_1 RGCY 4 cut(s) 29, 54, 78, 87
CviQI GTAC 1 cut(s) 23
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
DraIII CACNNNGTG 1 cut(s) 109
Eco147I AGGCCT 1 cut(s) 78
FaeI CATG 1 cut(s) 36
FaiI YATR 3 cut(s) 34, 59, 83
FaqI GGGAC 1 cut(s) 236
FatI CATG 1 cut(s) 32
FauI CCCGC 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 30, 55
FokI GGATG 2 cut(s) 81, 179
Fsp4HI GCNGC 2 cut(s) 30, 55
FspBI CTAG 2 cut(s) 11, 120
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 30, 55
GsaI CCCAGC 1 cut(s) 192
HaeIII GGCC 1 cut(s) 78
HapII CCGG 1 cut(s) 204
HhaI GCGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 36
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HinfI GANTC 2 cut(s) 183, 212
HpaII CCGG 1 cut(s) 204
HphI GGTGA 2 cut(s) 121, 127
Hpy166II GTNNAC 2 cut(s) 103, 201
Hpy188I TCNGA 1 cut(s) 165
Hpy8I GTNNAC 2 cut(s) 103, 201
HpyCH4V TGCA 5 cut(s) 17, 32, 57, 103, 201
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 198
Hsp92II CATG 1 cut(s) 36
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 169
LpnPI CCDG 7 cut(s) 11, 36, 60, 111, 202, 217, 228
Lsp1109I GCAGC 2 cut(s) 16, 41
LweI GCATC 2 cut(s) 4, 201
MaeI CTAG 2 cut(s) 11, 120
MaeIII GTNAC 2 cut(s) 46, 115
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 1 cut(s) 140
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 3 cut(s) 105, 179, 203
MluCI AATT 1 cut(s) 217
MnlI CCTC 1 cut(s) 105
MspI CCGG 1 cut(s) 204
MvnI CGCG 2 cut(s) 126, 181
MwoI GCNNNNNNNGC 2 cut(s) 84, 198
NdeII GATC 1 cut(s) 169
NlaIII CATG 1 cut(s) 36
NmuCI GTSAC 1 cut(s) 115
NspI RCATGY 1 cut(s) 36
PceI AGGCCT 1 cut(s) 78
PfeI GAWTC 2 cut(s) 183, 212
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 2 cut(s) 31, 56
PspFI CCCAGC 1 cut(s) 188
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SatI GCNGC 2 cut(s) 30, 55
Sau3AI GATC 1 cut(s) 169
SduI GDGCHC 3 cut(s) 105, 179, 203
SetI ASST 2 cut(s) 89, 111
SfaNI GCATC 2 cut(s) 4, 201
Sse9I AATT 1 cut(s) 217
SseBI AGGCCT 1 cut(s) 78
SsiI CCGC 1 cut(s) 179
SspMI CTAG 2 cut(s) 11, 120
StuI AGGCCT 1 cut(s) 78
TasI AATT 1 cut(s) 217
TfiI GAWTC 2 cut(s) 183, 212
TscAI CASTG 2 cut(s) 24, 105
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 2 cut(s) 29, 54
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 83
TspRI CASTG 2 cut(s) 24, 105
VneI GTGCAC 2 cut(s) 101, 199
XceI RCATGY 1 cut(s) 36
XspI CTAG 2 cut(s) 11, 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.