pycom05g29640

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
30037839 .. 30038414
576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGAAAAAGTCATATCCTTCTCTCTCATCACCCAGTCGTCGGCTTCTCTTGAAGCAAAGCAGAGGAGCAAAAGCACTTCCAACAATGGAGGTGCAAGGAATCCTCACCACCACCACACCAGAGCAAGACTACTACAATCTCCAGGGCATCAAATCCTCCAAATCCTTCTACACCTCCCCCAGAGGCCTCAAGCTCCTCACCCGCCAATGGATCCCCCTCCGTCGTCCACGTGGCATCATCTTCATGGTCCACGGCTACGGCTACGGCAATGACATCAGCTCGACCTTCCAAGCCACCCCGATCTTCCTCGCCCAAAACGGCTTCGCCTGCTTCGCTCTCGAGTCTCGACCTCGAGGGTCACGGCGGCCCCGACGGCCTCCGCGCCTTCGTCCCCGACGTTGGCCTCGTGGTCAACGACTGCCTATCGTTCTTCAATTTGGTCAAACAGCAGGACCCCCAATTTCATGGACTCCGCCGTTTTTTACACGGCGAGTCCATGGGCGGCGGGGCGGTTTGCCCCCCATGTGCAAAATCTCTGATAAAGTAAAACCCAGGTGGCCATTCCGCAGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

192

Amino Acids

22.04

Weight (kDa)

11.85

Isoelectric Point (pI)

70.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 351
AccII CGCG 1 cut(s) 382
AciI CCGC 8 cut(s) 202, 364, 380, 473, 502, 505, 510, 565
AclWI GGATC 2 cut(s) 205, 218
AcoI YGGCCR 1 cut(s) 557
AcvI CACGTG 1 cut(s) 230
AfiI CCNNNNNNNGG 3 cut(s) 39, 207, 399
AgsI TTSAA 2 cut(s) 52, 434
AjnI CCWGG 2 cut(s) 141, 551
AluBI AGCT 2 cut(s) 193, 279
AluI AGCT 2 cut(s) 193, 279
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 2 cut(s) 205, 218
AlwNI CAGNNNCTG 1 cut(s) 573
Ama87I CYCGRG 2 cut(s) 338, 351
AoxI GGCC 5 cut(s) 184, 365, 374, 401, 557
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 247, 366, 452
AsuHPI GGTGA 3 cut(s) 21, 97, 190
AvaI CYCGRG 2 cut(s) 338, 351
AvaII GGWCC 2 cut(s) 247, 452
BalI TGGCCA 1 cut(s) 559
BamHI GGATCC 1 cut(s) 210
BauI CACGAG 1 cut(s) 405
BbrPI CACGTG 1 cut(s) 230
BceAI ACGGC 8 cut(s) 268, 274, 280, 334, 377, 389, 460, 503
BciT130I CCWGG 2 cut(s) 143, 553
BcoDI GTCTC 1 cut(s) 348
BglI GCCNNNNNGGC 1 cut(s) 373
BisI GCNGC 2 cut(s) 365, 503
BlsI GCNGC 2 cut(s) 366, 504
Bme1390I CCNGG 2 cut(s) 143, 553
Bme18I GGWCC 2 cut(s) 247, 452
BmeT110I CYCGRG 2 cut(s) 338, 351
BmgT120I GGNCC 3 cut(s) 247, 366, 452
BmiI GGNNCC 3 cut(s) 212, 368, 454
BmrFI CCNGG 2 cut(s) 143, 553
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 156, 243
BmuI ACTGGG 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 173
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 4 cut(s) 142, 250, 496, 551
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 207, 399
Bse1I ACTGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 274
BseBI CCWGG 2 cut(s) 143, 553
BseDI CCNNGG 4 cut(s) 142, 250, 496, 551
BseLI CCNNNNNNNGG 3 cut(s) 39, 207, 399
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 1 cut(s) 33
BseRI GAGGAG 2 cut(s) 78, 185
Bsh1236I CGCG 1 cut(s) 382
BshFI GGCC 5 cut(s) 186, 367, 376, 403, 559
BsiHKCI CYCGRG 2 cut(s) 338, 351
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 39, 207, 399
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 375
BsnI GGCC 5 cut(s) 186, 367, 376, 403, 559
BsoBI CYCGRG 2 cut(s) 338, 351
Bsp143I GATC 2 cut(s) 210, 300
Bsp19I CCATGG 1 cut(s) 496
BspACI CCGC 8 cut(s) 202, 364, 380, 473, 502, 505, 510, 565
BspANI GGCC 5 cut(s) 186, 367, 376, 403, 559
BspFNI CGCG 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 212, 368, 454
BspPI GGATC 2 cut(s) 205, 218
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 4 cut(s) 142, 250, 496, 551
BssMI GATC 2 cut(s) 210, 300
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 496
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 2 cut(s) 143, 553
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 328
BstDSI CCRYGG 2 cut(s) 250, 496
BstFNI CGCG 1 cut(s) 382
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 2 cut(s) 213, 303
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 2 cut(s) 210, 300
BstMWI GCNNNNNNNGC 3 cut(s) 327, 332, 373
BstNI CCWGG 2 cut(s) 143, 553
BstSCI CCNGG 2 cut(s) 141, 551
BstUI CGCG 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 210
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 5 cut(s) 186, 367, 376, 403, 559
BtgI CCRYGG 2 cut(s) 250, 496
Cac8I GCNNGC 1 cut(s) 328
CaiI CAGNNNCTG 1 cut(s) 573
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 3 cut(s) 247, 366, 452
CviAII CATG 4 cut(s) 244, 465, 497, 523
DpnI GATC 2 cut(s) 212, 302
DpnII GATC 2 cut(s) 210, 300
EaeI YGGCCR 1 cut(s) 557
EciI GGCGGA 1 cut(s) 462
Eco130I CCWWGG 1 cut(s) 496
Eco147I AGGCCT 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 247, 452
Eco72I CACGTG 1 cut(s) 230
Eco88I CYCGRG 2 cut(s) 338, 351
EcoO109I RGGNCCY 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 141, 551
EcoT14I CCWWGG 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 4 cut(s) 247, 468, 500, 526
FaiI YATR 5 cut(s) 13, 245, 466, 498, 524
FaqI GGGAC 1 cut(s) 375
FatI CATG 4 cut(s) 243, 464, 496, 522
FauI CCCGC 2 cut(s) 209, 498
Fnu4HI GCNGC 2 cut(s) 365, 503
Fsp4HI GCNGC 2 cut(s) 365, 503
GlaI GCGC 1 cut(s) 383
GluI GCNGC 2 cut(s) 365, 503
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 5 cut(s) 186, 367, 376, 403, 559
HhaI GCGC 1 cut(s) 384
Hin1II CATG 4 cut(s) 247, 468, 500, 526
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 1 cut(s) 413
HindII GTYRAC 1 cut(s) 413
HinfI GANTC 4 cut(s) 99, 341, 469, 492
HphI GGTGA 3 cut(s) 21, 97, 190
Hpy166II GTNNAC 3 cut(s) 227, 250, 413
Hpy188I TCNGA 1 cut(s) 538
Hpy188III TCNNGA 3 cut(s) 49, 338, 345
Hpy8I GTNNAC 3 cut(s) 227, 250, 413
Hpy99I CGWCG 4 cut(s) 42, 225, 375, 399
HpyAV CCTTC 4 cut(s) 27, 175, 295, 395
HpyCH4IV ACGT 2 cut(s) 229, 397
HpyCH4V TGCA 2 cut(s) 94, 528
HpyF10VI GCNNNNNNNGC 3 cut(s) 327, 332, 373
HpySE526I ACGT 2 cut(s) 229, 397
Hsp92II CATG 4 cut(s) 247, 468, 500, 526
HspAI GCGC 1 cut(s) 382
Kzo9I GATC 2 cut(s) 210, 300
LmnI GCTCC 2 cut(s) 65, 198
LpnPI CCDG 9 cut(s) 46, 128, 132, 155, 193, 340, 435, 538, 565
LweI GCATC 2 cut(s) 156, 243
MaeII ACGT 2 cut(s) 229, 397
MaeIII GTNAC 1 cut(s) 357
MalI GATC 2 cut(s) 212, 302
MboI GATC 2 cut(s) 210, 300
MboII GAAGA 3 cut(s) 232, 295, 422
MflI RGATCY 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 559
MluCI AATT 2 cut(s) 434, 459
MluNI TGGCCA 1 cut(s) 559
MlyI GAGTC 3 cut(s) 350, 463, 501
MmeI TCCRAC 1 cut(s) 104
Mox20I TGGCCA 1 cut(s) 559
MscI TGGCCA 1 cut(s) 559
MslI CAYNNNNRTG 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 559
MspR9I CCNGG 2 cut(s) 143, 553
MvaI CCWGG 2 cut(s) 143, 553
MvnI CGCG 1 cut(s) 382
MwoI GCNNNNNNNGC 3 cut(s) 327, 332, 373
NcoI CCATGG 1 cut(s) 496
NdeII GATC 2 cut(s) 210, 300
NlaIII CATG 4 cut(s) 247, 468, 500, 526
NlaIV GGNNCC 3 cut(s) 212, 368, 454
NmuCI GTSAC 1 cut(s) 357
PaeR7I CTCGAG 2 cut(s) 338, 351
PceI AGGCCT 1 cut(s) 186
PcsI WCGNNNNNNNCGW 4 cut(s) 367, 394, 403, 412
PfeI GAWTC 1 cut(s) 99
PkrI GCNGC 2 cut(s) 366, 504
PleI GAGTC 3 cut(s) 349, 463, 500
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 3 cut(s) 349, 463, 500
Ppu21I YACGTR 1 cut(s) 230
PpuMI RGGWCCY 1 cut(s) 452
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 2 cut(s) 141, 551
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 2 cut(s) 141, 551
PspN4I GGNNCC 3 cut(s) 212, 368, 454
PspPI GGNCC 3 cut(s) 247, 366, 452
PspPPI RGGWCCY 1 cut(s) 452
PspXI VCTCGAGB 1 cut(s) 351
PstNI CAGNNNCTG 1 cut(s) 573
PsuI RGATCY 1 cut(s) 210
RseI CAYNNNNRTG 1 cut(s) 242
SatI GCNGC 2 cut(s) 365, 503
Sau3AI GATC 2 cut(s) 210, 300
Sau96I GGNCC 3 cut(s) 247, 366, 452
SchI GAGTC 3 cut(s) 350, 463, 501
ScrFI CCNGG 2 cut(s) 143, 553
SetI ASST 9 cut(s) 93, 176, 195, 232, 281, 287, 352, 400, 557
SfaNI GCATC 2 cut(s) 156, 243
SfiI GGCCNNNNNGGCC 1 cut(s) 373
Sfr274I CTCGAG 2 cut(s) 338, 351
SinI GGWCC 2 cut(s) 247, 452
SlaI CTCGAG 2 cut(s) 338, 351
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 3 cut(s) 188, 338, 351
SmoI CTYRAG 3 cut(s) 188, 338, 351
Sse9I AATT 2 cut(s) 434, 459
SseBI AGGCCT 1 cut(s) 186
SsiI CCGC 8 cut(s) 202, 364, 380, 473, 502, 505, 510, 565
StuI AGGCCT 1 cut(s) 186
StyD4I CCNGG 2 cut(s) 141, 551
StyI CCWWGG 1 cut(s) 496
TaiI ACGT 2 cut(s) 232, 400
TaqI TCGA 4 cut(s) 281, 339, 346, 352
TasI AATT 2 cut(s) 434, 459
TauI GCSGC 2 cut(s) 367, 505
TfiI GAWTC 1 cut(s) 99
TseFI GTSAC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 357
TspDTI ATGAA 3 cut(s) 17, 232, 453
TspGWI ACGGA 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 247, 452
XhoI CTCGAG 2 cut(s) 338, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.