Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
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No orthologs found for this gene in the current database.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 69 |
| AclWI | GGATC | 2 cut(s) | 16, 29 |
| AcsI | RAATTY | 3 cut(s) | 34, 153, 206 |
| AfaI | GTAC | 1 cut(s) | 182 |
| AgsI | TTSAA | 1 cut(s) | 190 |
| AjiI | CACGTC | 2 cut(s) | 18, 97 |
| AjnI | CCWGG | 1 cut(s) | 24 |
| Alw26I | GTCTC | 1 cut(s) | 100 |
| AlwI | GGATC | 2 cut(s) | 16, 29 |
| ApoI | RAATTY | 3 cut(s) | 34, 153, 206 |
| AseI | ATTAAT | 1 cut(s) | 89 |
| BamHI | GGATCC | 1 cut(s) | 21 |
| BauI | CACGAG | 1 cut(s) | 11 |
| BceAI | ACGGC | 2 cut(s) | 78, 90 |
| BciT130I | CCWGG | 1 cut(s) | 26 |
| BcoDI | GTCTC | 1 cut(s) | 100 |
| Bme1390I | CCNGG | 1 cut(s) | 26 |
| BmgBI | CACGTC | 2 cut(s) | 18, 97 |
| BmiI | GGNNCC | 1 cut(s) | 23 |
| BmrFI | CCNGG | 1 cut(s) | 26 |
| BmrI | ACTGGG | 1 cut(s) | 41 |
| BmsI | GCATC | 1 cut(s) | 31 |
| BmuI | ACTGGG | 1 cut(s) | 41 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 97, 127 |
| Bse1I | ACTGG | 1 cut(s) | 47 |
| BseBI | CCWGG | 1 cut(s) | 26 |
| BseGI | GGATG | 1 cut(s) | 46 |
| BseNI | ACTGG | 1 cut(s) | 47 |
| BsmAI | GTCTC | 1 cut(s) | 100 |
| Bsp143I | GATC | 1 cut(s) | 21 |
| BspACI | CCGC | 1 cut(s) | 69 |
| BspLI | GGNNCC | 1 cut(s) | 23 |
| BspPI | GGATC | 2 cut(s) | 16, 29 |
| BsrI | ACTGG | 1 cut(s) | 47 |
| BssMI | GATC | 1 cut(s) | 21 |
| BssSI | CACGAG | 1 cut(s) | 11 |
| Bst2BI | CACGAG | 1 cut(s) | 11 |
| Bst2UI | CCWGG | 1 cut(s) | 26 |
| BstC8I | GCNNGC | 1 cut(s) | 67 |
| BstF5I | GGATG | 1 cut(s) | 46 |
| BstKTI | GATC | 1 cut(s) | 24 |
| BstMAI | GTCTC | 1 cut(s) | 100 |
| BstMBI | GATC | 1 cut(s) | 21 |
| BstNI | CCWGG | 1 cut(s) | 26 |
| BstSCI | CCNGG | 1 cut(s) | 24 |
| BstX2I | RGATCY | 1 cut(s) | 21 |
| BstYI | RGATCY | 1 cut(s) | 21 |
| BtrI | CACGTC | 2 cut(s) | 18, 97 |
| BtsCI | GGATG | 1 cut(s) | 46 |
| BtsIMutI | CAGTG | 1 cut(s) | 54 |
| Cac8I | GCNNGC | 1 cut(s) | 67 |
| Csp6I | GTAC | 1 cut(s) | 181 |
| CviAII | CATG | 2 cut(s) | 110, 196 |
| CviJI | RGCY | 1 cut(s) | 65 |
| CviKI_1 | RGCY | 1 cut(s) | 65 |
| CviQI | GTAC | 1 cut(s) | 181 |
| DpnI | GATC | 1 cut(s) | 23 |
| DpnII | GATC | 1 cut(s) | 21 |
| EciI | GGCGGA | 1 cut(s) | 84 |
| EcoRII | CCWGG | 1 cut(s) | 24 |
| FaeI | CATG | 2 cut(s) | 113, 199 |
| FaiI | YATR | 8 cut(s) | 111, 116, 169, 173, 179, 197, 272, 277 |
| FatI | CATG | 2 cut(s) | 109, 195 |
| FokI | GGATG | 1 cut(s) | 53 |
| Hin1II | CATG | 2 cut(s) | 113, 199 |
| Hpy188III | TCNNGA | 2 cut(s) | 13, 190 |
| HpyCH4IV | ACGT | 2 cut(s) | 17, 96 |
| HpyCH4V | TGCA | 1 cut(s) | 261 |
| HpySE526I | ACGT | 2 cut(s) | 17, 96 |
| Hsp92II | CATG | 2 cut(s) | 113, 199 |
| Kzo9I | GATC | 1 cut(s) | 21 |
| LpnPI | CCDG | 5 cut(s) | 11, 38, 51, 60, 197 |
| LweI | GCATC | 1 cut(s) | 31 |
| MaeII | ACGT | 2 cut(s) | 17, 96 |
| MaeIII | GTNAC | 2 cut(s) | 13, 239 |
| MalI | GATC | 1 cut(s) | 23 |
| MboI | GATC | 1 cut(s) | 21 |
| MboII | GAAGA | 1 cut(s) | 65 |
| MflI | RGATCY | 1 cut(s) | 21 |
| MluCI | AATT | 3 cut(s) | 34, 153, 206 |
| MnlI | CCTC | 1 cut(s) | 192 |
| MseI | TTAA | 2 cut(s) | 89, 210 |
| MspR9I | CCNGG | 1 cut(s) | 26 |
| MvaI | CCWGG | 1 cut(s) | 26 |
| NdeII | GATC | 1 cut(s) | 21 |
| NlaIII | CATG | 2 cut(s) | 113, 199 |
| NlaIV | GGNNCC | 1 cut(s) | 23 |
| NmuCI | GTSAC | 1 cut(s) | 13 |
| PshBI | ATTAAT | 1 cut(s) | 89 |
| Psp6I | CCWGG | 1 cut(s) | 24 |
| PspGI | CCWGG | 1 cut(s) | 24 |
| PspN4I | GGNNCC | 1 cut(s) | 23 |
| PsuI | RGATCY | 1 cut(s) | 21 |
| RsaI | GTAC | 1 cut(s) | 182 |
| RsaNI | GTAC | 1 cut(s) | 181 |
| SaqAI | TTAA | 2 cut(s) | 89, 210 |
| Sau3AI | GATC | 1 cut(s) | 21 |
| ScrFI | CCNGG | 1 cut(s) | 26 |
| SetI | ASST | 5 cut(s) | 20, 30, 99, 186, 203 |
| SfaNI | GCATC | 1 cut(s) | 31 |
| Sse9I | AATT | 3 cut(s) | 34, 153, 206 |
| SsiI | CCGC | 1 cut(s) | 69 |
| StyD4I | CCNGG | 1 cut(s) | 24 |
| TaiI | ACGT | 2 cut(s) | 20, 99 |
| TasI | AATT | 3 cut(s) | 34, 153, 206 |
| Tru1I | TTAA | 2 cut(s) | 89, 210 |
| Tru9I | TTAA | 2 cut(s) | 89, 210 |
| TscAI | CASTG | 1 cut(s) | 54 |
| TseFI | GTSAC | 1 cut(s) | 13 |
| Tsp45I | GTSAC | 1 cut(s) | 13 |
| TspDTI | ATGAA | 1 cut(s) | 75 |
| TspRI | CASTG | 1 cut(s) | 54 |
| VspI | ATTAAT | 1 cut(s) | 89 |
| XapI | RAATTY | 3 cut(s) | 34, 153, 206 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 54 |