pycom05g32920

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
32127917 .. 32130447
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGGAGAAAGCTGGTTGTCTTAACTACTCTTACACCCCATGGGAATCAGAGAAGGGCGATGTCTGTGTGAGGCAAATATATTACAGATTTGATAAACGTGTGTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGCCTCTCTGATAGAAATGGTTGGCTTGTACAAGAGGTCTCGACCCTCCATGCAGTACCGCTTGGCGACTATTTCAATCTTCACGTTCGATACCAAATCGAGGATTTGCCCTCAACACCAAAGGGATGCCAGCAGCTGTACCTCGCTGTTGGTGGTGGCAGCCCGGGTGGTGCTTATTCTTCTAGAATAGCTCAAACTTCACCTGCAGAGGTTAATCTGCCACCACCTCCCGATCCGTCTGATGAAGACGCTGTTGTGGCTTATAATACGCTTCTAAATCGTAATGGTCTGACCACACCTGACGTAGTCCCTAAGTCCCGCAAGCCTAGTGACTCAGCAGGCGCAAAAGGAGACAACCAGCAGCCAGAGAGGCCCGCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

176

Amino Acids

19.27

Weight (kDa)

6.12

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VASt PF16016 1 - 86 5.2e-13 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AarI CACCTGC 1 cut(s) 355
Acc36I ACCTGC 1 cut(s) 355
AciI CCGC 4 cut(s) 145, 203, 463, 519
AclWI GGATC 1 cut(s) 371
AfaI GTAC 4 cut(s) 130, 174, 201, 284
AfiI CCNNNNNNNGG 1 cut(s) 244
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 1 cut(s) 220
AhlI ACTAGT 1 cut(s) 125
AluBI AGCT 3 cut(s) 11, 280, 335
AluI AGCT 3 cut(s) 11, 280, 335
Alw26I GTCTC 2 cut(s) 187, 489
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 280
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 1 cut(s) 515
ApeKI GCWGC 3 cut(s) 277, 303, 505
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 516
AsuC2I CCSGG 2 cut(s) 308, 309
AsuHPI GGTGA 2 cut(s) 133, 336
AvaI CYCGRG 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 396
BbvI GCAGC 3 cut(s) 289, 315, 517
BcnI CCSGG 2 cut(s) 308, 309
BcoDI GTCTC 2 cut(s) 187, 489
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 3 cut(s) 126, 327, 471
BfmI CTRYAG 1 cut(s) 348
BfuAI ACCTGC 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 514
BisI GCNGC 3 cut(s) 278, 304, 506
BlsI GCNGC 3 cut(s) 279, 305, 507
BmcAI AGTACT 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 308, 309
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 516
BmrFI CCNGG 2 cut(s) 308, 309
BmsI GCATC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 396
BpuMI CCSGG 2 cut(s) 308, 309
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 38, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 38, 307
BseGI GGATG 1 cut(s) 275
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 2 cut(s) 146, 492
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 289, 315, 517
BshFI GGCC 1 cut(s) 517
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 3 cut(s) 88, 437, 445
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 2 cut(s) 187, 489
BsmFI GGGAC 3 cut(s) 88, 437, 445
BsnI GGCC 1 cut(s) 517
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 307
Bsp1407I TGTACA 1 cut(s) 172
Bsp143I GATC 1 cut(s) 376
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 4 cut(s) 145, 203, 463, 519
BspANI GGCC 1 cut(s) 517
BspCNI CTCAG 2 cut(s) 145, 491
BspMAI CTGCAG 1 cut(s) 352
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 172
BssECI CCNNGG 2 cut(s) 38, 307
BssMI GATC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 38
BstAUI TGTACA 1 cut(s) 172
BstC8I GCNNGC 4 cut(s) 275, 467, 484, 519
BstDEI CTNAG 3 cut(s) 132, 456, 478
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 275
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 2 cut(s) 187, 489
BstMBI GATC 1 cut(s) 376
BstMWI GCNNNNNNNGC 2 cut(s) 401, 514
BstSCI CCNGG 2 cut(s) 306, 307
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 289, 315, 517
BstV2I GAAGAC 1 cut(s) 396
BsuRI GGCC 1 cut(s) 517
BtgI CCRYGG 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 275
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 4 cut(s) 275, 467, 484, 519
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 516
Cfr9I CCCGGG 1 cut(s) 307
CseI GACGC 1 cut(s) 401
Csp6I GTAC 4 cut(s) 129, 173, 200, 283
CviAII CATG 2 cut(s) 39, 194
CviJI RGCY 9 cut(s) 11, 169, 280, 306, 335, 404, 469, 508, 517
CviKI_1 RGCY 9 cut(s) 11, 169, 280, 306, 335, 404, 469, 508, 517
CviQI GTAC 4 cut(s) 129, 173, 200, 283
DdeI CTNAG 3 cut(s) 132, 456, 478
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 187
Eco88I CYCGRG 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 2 cut(s) 42, 197
FaiI YATR 5 cut(s) 40, 79, 111, 195, 408
FaqI GGGAC 3 cut(s) 88, 437, 445
FatI CATG 2 cut(s) 38, 193
FauI CCCGC 3 cut(s) 152, 470, 526
Fnu4HI GCNGC 3 cut(s) 278, 304, 506
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 3 cut(s) 278, 304, 506
FspBI CTAG 3 cut(s) 126, 327, 471
GlaI GCGC 1 cut(s) 487
GluI GCNGC 3 cut(s) 278, 304, 506
HaeIII GGCC 1 cut(s) 517
HapII CCGG 1 cut(s) 308
HgaI GACGC 1 cut(s) 401
HhaI GCGC 1 cut(s) 488
Hin1II CATG 2 cut(s) 42, 197
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HinfI GANTC 2 cut(s) 44, 476
HpaII CCGG 1 cut(s) 308
HphI GGTGA 2 cut(s) 133, 336
Hpy188I TCNGA 4 cut(s) 49, 154, 385, 435
Hpy188III TCNNGA 3 cut(s) 184, 327, 374
HpyAV CCTTC 1 cut(s) 46
HpyCH4IV ACGT 3 cut(s) 97, 228, 447
HpyCH4V TGCA 2 cut(s) 197, 350
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 514
HpyF3I CTNAG 3 cut(s) 132, 456, 478
HpySE526I ACGT 3 cut(s) 97, 228, 447
Hsp92II CATG 2 cut(s) 42, 197
HspAI GCGC 1 cut(s) 486
Kzo9I GATC 1 cut(s) 376
LpnPI CCDG 7 cut(s) 287, 321, 360, 456, 468, 515, 522
Lsp1109I GCAGC 3 cut(s) 289, 315, 517
LweI GCATC 1 cut(s) 260
MaeI CTAG 3 cut(s) 126, 327, 471
MaeII ACGT 3 cut(s) 97, 228, 447
MaeIII GTNAC 2 cut(s) 121, 473
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 215, 315, 401
MlyI GAGTC 1 cut(s) 470
MseI TTAA 2 cut(s) 21, 357
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 2 cut(s) 308, 309
MwoI GCNNNNNNNGC 2 cut(s) 401, 514
NciI CCSGG 2 cut(s) 308, 309
NcoI CCATGG 1 cut(s) 38
NdeII GATC 1 cut(s) 376
NlaIII CATG 2 cut(s) 42, 197
NmuCI GTSAC 2 cut(s) 121, 473
PaqCI CACCTGC 1 cut(s) 355
PfeI GAWTC 1 cut(s) 44
PflFI GACNNNGTC 1 cut(s) 449
PkrI GCNGC 3 cut(s) 279, 305, 507
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PsiI TTATAA 1 cut(s) 408
PspPI GGNCC 1 cut(s) 516
PstI CTGCAG 1 cut(s) 352
PstNI CAGNNNCTG 1 cut(s) 280
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 4 cut(s) 130, 174, 201, 284
RsaNI GTAC 4 cut(s) 129, 173, 200, 283
SaqAI TTAA 2 cut(s) 21, 357
SatI GCNGC 3 cut(s) 278, 304, 506
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 516
ScaI AGTACT 1 cut(s) 130
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 308, 309
SfaNI GCATC 1 cut(s) 260
SfcI CTRYAG 1 cut(s) 348
SmaI CCCGGG 1 cut(s) 309
SpeI ACTAGT 1 cut(s) 125
SsiI CCGC 4 cut(s) 145, 203, 463, 519
SspMI CTAG 3 cut(s) 126, 327, 471
StyD4I CCNGG 2 cut(s) 306, 307
StyI CCWWGG 1 cut(s) 38
TaiI ACGT 3 cut(s) 100, 231, 450
TaqI TCGA 3 cut(s) 185, 232, 243
TatI WGTACW 2 cut(s) 128, 172
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 2 cut(s) 21, 357
Tru9I TTAA 2 cut(s) 21, 357
TseFI GTSAC 2 cut(s) 121, 473
TseI GCWGC 3 cut(s) 277, 303, 505
Tsp45I GTSAC 2 cut(s) 121, 473
TspDTI ATGAA 1 cut(s) 402
TspGWI ACGGA 1 cut(s) 369
TspMI CCCGGG 1 cut(s) 307
Tth111I GACNNNGTC 1 cut(s) 449
XbaI TCTAGA 1 cut(s) 326
XmaI CCCGGG 1 cut(s) 307
XspI CTAG 3 cut(s) 126, 327, 471
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.