pycom06g04370

deSI-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
5624816 .. 5627492
2677 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGGGAGAAGAAGGAACGGGGAAGGAAGGAGAGAAGATTATTCGGAATAACTTTGTAGAAATCTTCACTTCGCCGAGGTTTGTTTCGATCGGCGAGCCGATTCAATATACGATGCGGTTGTTTCCTTTGAGCTCAACGGGAAAAAAATCGCGCCCTTTTGTACCTCAATGTCTACGATCTCACGCCTGTGAACAACTACCTTTACTGGGTTTGATTCGGTCGATCTTCCACTCCGGCATAGAAGTTCATGGCATGGAGTATGGCTTTGGAGCCCATGAGTATCCCAGCAGTGGGGTTTTTGAGGTGGAACCAAGAAGTTGTCCTGGCTTCATCTTCAGAAGGTCAGTCCTGTTGGGAAGCACTGAAATGTCTCGCTCAGAAGTTCGGTCATTTATGGAGCATCTTTCTGGAAAGTATCGTGGAGATACCTATCATTTAATTGCCAAGAATTGCAACCATTTCACTGATGAAGTTTGTATGCAGCTAACTGGAAAACCTATACCTGGATGGGTAAATCGGCTGGCCCAAGTAGGTTCTTTCTGCAACTGTCTTCTCCCAGAAAACATTCAAATTTCAGCAGTGAGACATCTTCCTGACCATCCAGCATATTCTGGTATGTTCCCATTGGAAAAAAAAAAAAAAATGTTCTTTAAAGAGTTTTGCATCACAATCTTGCATATTCTTTCTGACTTCCTTTTCTGCAATATAGTTATACTTCCTCTTATGGGACTCAATCTTCTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C97

Protein Analysis

249

Amino Acids

28.05

Weight (kDa)

8.36

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C97 PF05903 68 - 188 7.9e-47 PPPDE putative peptidase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 172
AccII CGCG 1 cut(s) 151
AciI CCGC 1 cut(s) 115
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 319
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 5 cut(s) 206, 290, 291, 503, 725
AgsI TTSAA 2 cut(s) 104, 569
AjnI CCWGG 2 cut(s) 322, 502
AleI CACNNNNGTG 1 cut(s) 186
AluBI AGCT 2 cut(s) 132, 484
AluI AGCT 2 cut(s) 132, 484
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 375, 577
AoxI GGCC 1 cut(s) 522
ApeKI GCWGC 1 cut(s) 481
ApoI RAATTY 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 564
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 523
BanII GRGCYC 2 cut(s) 134, 274
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 493
BccI CCATC 2 cut(s) 501, 606
BciT130I CCWGG 2 cut(s) 324, 504
BciVI GTATCC 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 375, 577
BfuI GTATCC 1 cut(s) 291
BisI GCNGC 1 cut(s) 482
BlsI GCNGC 1 cut(s) 483
Bme1390I CCNGG 2 cut(s) 324, 504
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 2 cut(s) 271, 309
BmrFI CCNGG 2 cut(s) 324, 504
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 3 cut(s) 102, 409, 672
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 542
BsaBI GATNNNNATC 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 74
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 290, 291, 503, 725
Bse1I ACTGG 2 cut(s) 210, 493
Bse8I GATNNNNATC 1 cut(s) 429
BseBI CCWGG 2 cut(s) 324, 504
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 2 cut(s) 512, 598
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 5 cut(s) 206, 290, 291, 503, 725
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 2 cut(s) 210, 493
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 151
Bsh1285I CGRYCG 2 cut(s) 90, 221
BshFI GGCC 1 cut(s) 524
BsiEI CGRYCG 2 cut(s) 90, 221
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 5 cut(s) 206, 290, 291, 503, 725
BsmAI GTCTC 2 cut(s) 375, 577
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 1 cut(s) 524
Bsp1286I GDGCHC 2 cut(s) 134, 274
Bsp143I GATC 3 cut(s) 87, 176, 222
BspACI CCGC 1 cut(s) 115
BspANI GGCC 1 cut(s) 524
BspCNI CTCAG 1 cut(s) 389
BspFNI CGCG 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 271, 309
BsrI ACTGG 2 cut(s) 210, 493
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 3 cut(s) 87, 176, 222
Bst2UI CCWGG 2 cut(s) 324, 504
Bst4CI ACNGT 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 95, 522
BstDEI CTNAG 1 cut(s) 376
BstF5I GGATG 2 cut(s) 512, 598
BstFNI CGCG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 3 cut(s) 90, 179, 225
BstMAI GTCTC 2 cut(s) 375, 577
BstMBI GATC 3 cut(s) 87, 176, 222
BstMCI CGRYCG 2 cut(s) 90, 221
BstNI CCWGG 2 cut(s) 324, 504
BstSCI CCNGG 2 cut(s) 322, 502
BstUI CGCG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 493
BstV2I GAAGAC 1 cut(s) 542
BsuI GTATCC 1 cut(s) 291
BsuRI GGCC 1 cut(s) 524
BtsCI GGATG 2 cut(s) 512, 598
BtsI GCAGTG 2 cut(s) 295, 585
BtsIMutI CAGTG 4 cut(s) 295, 360, 462, 585
Cac8I GCNNGC 2 cut(s) 95, 522
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 523
Csp6I GTAC 1 cut(s) 161
CviAII CATG 3 cut(s) 248, 253, 275
CviJI RGCY 8 cut(s) 97, 132, 264, 272, 327, 484, 520, 524
CviKI_1 RGCY 8 cut(s) 97, 132, 264, 272, 327, 484, 520, 524
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 1 cut(s) 376
DpnI GATC 3 cut(s) 89, 178, 224
DpnII GATC 3 cut(s) 87, 176, 222
DraI TTTAAA 1 cut(s) 652
Ecl136II GAGCTC 1 cut(s) 132
Eco24I GRGCYC 2 cut(s) 134, 274
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 322, 502
EcoT38I GRGCYC 2 cut(s) 134, 274
FaeI CATG 3 cut(s) 251, 256, 278
FaqI GGGAC 1 cut(s) 741
FatI CATG 3 cut(s) 247, 252, 274
FblI GTMKAC 1 cut(s) 172
Fnu4HI GCNGC 1 cut(s) 482
FokI GGATG 2 cut(s) 519, 585
FriOI GRGCYC 2 cut(s) 134, 274
Fsp4HI GCNGC 1 cut(s) 482
GlaI GCGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 482
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 524
HapII CCGG 1 cut(s) 234
HhaI GCGC 1 cut(s) 153
Hin1II CATG 3 cut(s) 251, 256, 278
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 3 cut(s) 100, 214, 729
HpaII CCGG 1 cut(s) 234
Hpy166II GTNNAC 2 cut(s) 173, 191
Hpy188I TCNGA 4 cut(s) 45, 338, 379, 688
Hpy188III TCNNGA 3 cut(s) 408, 593, 744
Hpy8I GTNNAC 2 cut(s) 173, 191
HpyAV CCTTC 4 cut(s) 5, 16, 20, 333
HpyCH4III ACNGT 1 cut(s) 548
HpyCH4V TGCA 6 cut(s) 453, 481, 543, 663, 676, 702
HpyF3I CTNAG 1 cut(s) 376
Hsp92II CATG 3 cut(s) 251, 256, 278
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 3 cut(s) 87, 176, 222
LmnI GCTCC 2 cut(s) 269, 397
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 3 cut(s) 102, 409, 672
MalI GATC 3 cut(s) 89, 178, 224
MboI GATC 3 cut(s) 87, 176, 222
MboII GAAGA 8 cut(s) 20, 46, 55, 217, 325, 542, 581, 728
MhlI GDGCHC 2 cut(s) 134, 274
MluCI AATT 3 cut(s) 438, 448, 570
MlyI GAGTC 1 cut(s) 723
MnlI CCTC 4 cut(s) 69, 174, 295, 729
MroXI GAANNNNTTC 1 cut(s) 564
MseI TTAA 2 cut(s) 437, 651
MslI CAYNNNNRTG 2 cut(s) 186, 365
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 324, 504
MvaI CCWGG 2 cut(s) 324, 504
MvnI CGCG 1 cut(s) 151
NdeII GATC 3 cut(s) 87, 176, 222
NlaIII CATG 3 cut(s) 251, 256, 278
NlaIV GGNNCC 2 cut(s) 271, 309
NmeAIII GCCGAG 1 cut(s) 99
OliI CACNNNNGTG 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 2 cut(s) 100, 214
PkrI GCNGC 1 cut(s) 483
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 1 cut(s) 723
PpsI GAGTC 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 134
Psp6I CCWGG 2 cut(s) 322, 502
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 322, 502
PspN4I GGNNCC 2 cut(s) 271, 309
PspPI GGNCC 1 cut(s) 523
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
RseI CAYNNNNRTG 2 cut(s) 186, 365
SacI GAGCTC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 437, 651
SatI GCNGC 1 cut(s) 482
Sau3AI GATC 3 cut(s) 87, 176, 222
Sau96I GGNCC 1 cut(s) 523
SchI GAGTC 1 cut(s) 723
ScrFI CCNGG 2 cut(s) 324, 504
SduI GDGCHC 2 cut(s) 134, 274
SfaNI GCATC 3 cut(s) 102, 409, 672
SmiMI CAYNNNNRTG 2 cut(s) 186, 365
Sse9I AATT 3 cut(s) 438, 448, 570
SsiI CCGC 1 cut(s) 115
SstI GAGCTC 1 cut(s) 134
StyD4I CCNGG 2 cut(s) 322, 502
TaaI ACNGT 1 cut(s) 548
TaqI TCGA 2 cut(s) 86, 221
TaqII GACCGA 2 cut(s) 207, 375
TasI AATT 3 cut(s) 438, 448, 570
TfiI GAWTC 2 cut(s) 100, 214
Tru1I TTAA 2 cut(s) 437, 651
Tru9I TTAA 2 cut(s) 437, 651
TscAI CASTG 4 cut(s) 295, 367, 469, 585
TseI GCWGC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 236, 319, 483
TspRI CASTG 4 cut(s) 295, 367, 469, 585
XapI RAATTY 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.