pycom06g09080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
14257668 .. 14259804
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGTTTGACTCTAAACATTGGTGGTTTTTAATATTATTGGTTTCTTTTCAATTGTGTATTCAAGCTTCTTATAAAGTAAAACAAGAAAATGAGTTTTTTAATGGTTCATGGATTTTAAGTTGGTTTTGTTTTATTATATTTTCAAATATTTTAAATCCTCAAAATGAAAACTTTAAAAAAACAGGACTGCCTTTTGTGATGTATACACTAGAACCGCCACAGGTTCAAGTACGATTGATATTTCTTGATGGATACCGAGTCAGTAGATATTCGTTCTTAGCTCCACCAGACCTTGGCCGGCCCACTTGGGCGATCATATTCCGCATGCCACCAAAATGGACTAATTCAAACGTTCTAGTACGCCCGACTTTTCACGAACTTCTCTGCCATAAGGGGCATATAGTTTTAATACGACAACAACCAAGTGGGATAAAGACTTGGAATTCGCCTAATATCACTTTTTCAAGAAAAAGTAATGAAACGATTATAAATTACATTTCAACTGTTGAACGACATAACTACAAATTGAAACTACTCATTTTTTTAACAAATGATATTATCTACAATAGGAAAGATGGTGGGCTTATGAAAGTCAAACATAAAACATCTCATTTACAAGTGAAGAGGAATATCACTAGACGATATTTTTTTCTTTTAATTAAACGAATTAGATATTTGAAAACTGAACACTCTGCTTTGTGGCGACGCCACACTTTTAATGTGATTCTTGTGCTAAAAATGTTATTAATTAAACACTTGTATCTTCCAATAAAAAAAAATAACAGGACGTGTCACGTGGATATGTTAACCCAAATTCTTATGCCCCATCAAAGCTTCTATGGATATAATAAGGATTCTCAATTTTATATTTCAAATGCAATACTATCTCCAAAGAATTGGAGATGTCACATTTTAAACCTAAAATTTAAATTTGATAGCTCACGTGGCATTTTGATATATTTTCATGTTTCACTCTCCACCCGATATGTCAAATTAAACTATTATTTTATTACATTATTTTTTTTTCTTAATTTTTGTCTTATTTTATTACATTATGTTTTGCTCTCCTCCGCTGACTCGCCTTCTAATCCCCTTCTGCACAAGCTCAAGTACGCCATCCACCAGATCATCGTATCCGCGCCCGATTGGTTCCCCTTTTTGCCCGGCGCCTCCTACTGGGTCCCTCCTCCACGGTCGAGATTGCATGGCCTGGCTCAACCATGTCTATGGGCACCGTTAGAGGGTGGCCTTCTTCTGCTTATTTTATCAAAGGTTTGCTTCCCTTTTTTCGATTTTATTAATTGGGTTCTGTTCAGAATTGGGTTTTTAGCCTTCAATCAATTGAGATTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

452

Amino Acids

53.59

Weight (kDa)

10.02

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 72, 488
AccB1I GGYRCC 2 cut(s) 1166, 1231
AccB7I CCANNNNNTGG 1 cut(s) 293
AccI GTMKAC 1 cut(s) 203
AccII CGCG 1 cut(s) 1139
AciI CCGC 4 cut(s) 215, 322, 1071, 1137
AclI AACGTT 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 4 cut(s) 442, 813, 923, 929
AcvI CACGTG 2 cut(s) 796, 944
AcyI GRCGYC 2 cut(s) 706, 1167
AfaI GTAC 3 cut(s) 231, 360, 1112
AfiI CCNNNNNNNGG 3 cut(s) 293, 1176, 1241
AflIII ACRYGT 1 cut(s) 788
AjiI CACGTC 1 cut(s) 789
AjnI CCWGG 1 cut(s) 1209
AluBI AGCT 5 cut(s) 65, 281, 834, 939, 1105
AluI AGCT 5 cut(s) 65, 281, 834, 939, 1105
AoxI GGCC 4 cut(s) 295, 299, 1207, 1246
ApoI RAATTY 4 cut(s) 442, 813, 923, 929
ArsI GACNNNNNNTTYG 2 cut(s) 427, 459
AseI ATTAAT 2 cut(s) 746, 1299
AspLEI GCGC 2 cut(s) 1141, 1169
AspS9I GGNCC 2 cut(s) 300, 1180
AsuC2I CCSGG 1 cut(s) 1164
AvaII GGWCC 1 cut(s) 1180
BaeGI GKGCMC 1 cut(s) 1234
BaeI ACNNNNGTAYC 2 cut(s) 743, 776
BanI GGYRCC 2 cut(s) 1166, 1231
BarI GAAGNNNNNNTAC 2 cut(s) 49, 81
BbrPI CACGTG 2 cut(s) 796, 944
BccI CCATC 4 cut(s) 242, 569, 834, 1124
BciT130I CCWGG 1 cut(s) 1211
BciVI GTATCC 2 cut(s) 245, 1144
BcnI CCSGG 1 cut(s) 1164
BfaI CTAG 3 cut(s) 209, 356, 636
BfoI RGCGCY 1 cut(s) 1170
BfuI GTATCC 2 cut(s) 245, 1144
Bme1390I CCNGG 2 cut(s) 1164, 1211
Bme18I GGWCC 1 cut(s) 1180
BmgBI CACGTC 1 cut(s) 789
BmgT120I GGNCC 2 cut(s) 300, 1180
BmiI GGNNCC 5 cut(s) 1151, 1168, 1181, 1182, 1233
BmrFI CCNGG 2 cut(s) 1164, 1211
BmrI ACTGGG 1 cut(s) 1186
BmuI ACTGGG 1 cut(s) 1186
BpuEI CTTGAG 1 cut(s) 1091
BpuMI CCSGG 1 cut(s) 1164
BsaAI YACGTR 2 cut(s) 796, 944
BsaHI GRCGYC 2 cut(s) 706, 1167
BsaJI CCNNGG 2 cut(s) 292, 1190
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 1176, 1241
Bse118I RCCGGY 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 1181
BseBI CCWGG 1 cut(s) 1211
BseDI CCNNGG 2 cut(s) 292, 1190
BseGI GGATG 1 cut(s) 1116
BseLI CCNNNNNNNGG 3 cut(s) 293, 1176, 1241
BseNI ACTGG 1 cut(s) 1181
BseRI GAGGAG 2 cut(s) 1057, 1176
BseSI GKGCMC 1 cut(s) 1234
BsgI GTGCAG 1 cut(s) 1082
Bsh1236I CGCG 1 cut(s) 1139
Bsh1285I CGRYCG 1 cut(s) 1196
BshFI GGCC 4 cut(s) 297, 301, 1209, 1248
BshNI GGYRCC 2 cut(s) 1166, 1231
BsiEI CGRYCG 1 cut(s) 1196
BsiSI CCGG 2 cut(s) 298, 1164
BslFI GGGAC 1 cut(s) 1166
BslI CCNNNNNNNGG 3 cut(s) 293, 1176, 1241
BsmFI GGGAC 1 cut(s) 1166
BsnI GGCC 4 cut(s) 297, 301, 1209, 1248
Bsp1286I GDGCHC 1 cut(s) 1234
Bsp143I GATC 2 cut(s) 312, 1125
BspACI CCGC 4 cut(s) 215, 322, 1071, 1137
BspANI GGCC 4 cut(s) 297, 301, 1209, 1248
BspFNI CGCG 1 cut(s) 1139
BspLI GGNNCC 5 cut(s) 1151, 1168, 1181, 1182, 1233
BspT107I GGYRCC 2 cut(s) 1166, 1231
BsrFI RCCGGY 1 cut(s) 297
BsrI ACTGG 1 cut(s) 1181
BssAI RCCGGY 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 292, 1190
BssMI GATC 2 cut(s) 312, 1125
BssNAI GTATAC 1 cut(s) 204
BssNI GRCGYC 2 cut(s) 706, 1167
BssT1I CCWWGG 1 cut(s) 292
Bst1107I GTATAC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 1211
Bst4CI ACNGT 3 cut(s) 505, 1194, 1236
Bst6I CTCTTC 1 cut(s) 617
BstACI GRCGYC 2 cut(s) 706, 1167
BstBAI YACGTR 2 cut(s) 796, 944
BstC8I GCNNGC 2 cut(s) 299, 326
BstDEI CTNAG 1 cut(s) 277
BstDSI CCRYGG 1 cut(s) 1190
BstF5I GGATG 1 cut(s) 1116
BstFNI CGCG 1 cut(s) 1139
BstH2I RGCGCY 1 cut(s) 1170
BstHHI GCGC 2 cut(s) 1141, 1169
BstKTI GATC 2 cut(s) 315, 1128
BstMBI GATC 2 cut(s) 312, 1125
BstMCI CGRYCG 1 cut(s) 1196
BstMWI GCNNNNNNNGC 1 cut(s) 945
BstNI CCWGG 1 cut(s) 1211
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 1162, 1209
BstSLI GKGCMC 1 cut(s) 1234
BstUI CGCG 1 cut(s) 1139
BstXI CCANNNNNNTGG 3 cut(s) 336, 897, 1227
BstZ17I GTATAC 1 cut(s) 204
BsuI GTATCC 2 cut(s) 245, 1144
BsuRI GGCC 4 cut(s) 297, 301, 1209, 1248
BtgI CCRYGG 1 cut(s) 1190
BtrI CACGTC 1 cut(s) 789
BtsCI GGATG 1 cut(s) 1116
Cac8I GCNNGC 2 cut(s) 299, 326
CfoI GCGC 2 cut(s) 1141, 1169
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 2 cut(s) 300, 1180
CseI GACGC 1 cut(s) 714
Csp6I GTAC 3 cut(s) 230, 359, 1111
CviAII CATG 5 cut(s) 108, 325, 965, 1205, 1221
CviQI GTAC 3 cut(s) 230, 359, 1111
DdeI CTNAG 1 cut(s) 277
DinI GGCGCC 1 cut(s) 1168
DpnI GATC 2 cut(s) 314, 1127
DpnII GATC 2 cut(s) 312, 1125
DraI TTTAAA 4 cut(s) 153, 175, 915, 928
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 1180
Eco72I CACGTG 2 cut(s) 796, 944
EcoO109I RGGNCCY 1 cut(s) 1180
EcoRI GAATTC 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 1209
EcoT14I CCWWGG 1 cut(s) 292
EgeI GGCGCC 1 cut(s) 1168
EheI GGCGCC 1 cut(s) 1168
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 5 cut(s) 111, 328, 968, 1208, 1224
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 1 cut(s) 1166
FatI CATG 5 cut(s) 107, 324, 964, 1204, 1220
FblI GTMKAC 1 cut(s) 203
FokI GGATG 1 cut(s) 1103
FseI GGCCGGCC 1 cut(s) 301
FspBI CTAG 3 cut(s) 209, 356, 636
GlaI GCGC 2 cut(s) 1140, 1168
HaeII RGCGCY 1 cut(s) 1170
HaeIII GGCC 4 cut(s) 297, 301, 1209, 1248
HapII CCGG 2 cut(s) 298, 1164
HgaI GACGC 1 cut(s) 714
HhaI GCGC 2 cut(s) 1141, 1169
Hin1I GRCGYC 2 cut(s) 706, 1167
Hin1II CATG 5 cut(s) 111, 328, 968, 1208, 1224
Hin6I GCGC 2 cut(s) 1139, 1167
HinP1I GCGC 2 cut(s) 1139, 1167
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HindIII AAGCTT 2 cut(s) 63, 832
HinfI GANTC 5 cut(s) 8, 258, 724, 854, 1076
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 2 cut(s) 298, 1164
Hpy166II GTNNAC 2 cut(s) 204, 807
Hpy188I TCNGA 1 cut(s) 1316
Hpy188III TCNNGA 4 cut(s) 245, 374, 465, 1197
Hpy8I GTNNAC 2 cut(s) 204, 807
Hpy99I CGWCG 1 cut(s) 708
HpyAV CCTTC 4 cut(s) 1092, 1103, 1259, 1342
HpyCH4III ACNGT 3 cut(s) 505, 1194, 1236
HpyCH4IV ACGT 4 cut(s) 351, 788, 795, 943
HpyCH4V TGCA 3 cut(s) 878, 1099, 1204
HpyF10VI GCNNNNNNNGC 1 cut(s) 945
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 4 cut(s) 351, 788, 795, 943
Hsp92I GRCGYC 2 cut(s) 706, 1167
Hsp92II CATG 5 cut(s) 111, 328, 968, 1208, 1224
HspAI GCGC 2 cut(s) 1139, 1167
KasI GGCGCC 1 cut(s) 1166
KflI GGGWCCC 1 cut(s) 1180
KroI GCCGGC 1 cut(s) 297
KroNI GCCGGC 1 cut(s) 299
KspAI GTTAAC 1 cut(s) 807
Kzo9I GATC 2 cut(s) 312, 1125
LmnI GCTCC 1 cut(s) 286
MaeI CTAG 3 cut(s) 209, 356, 636
MaeII ACGT 4 cut(s) 351, 788, 795, 943
MaeIII GTNAC 2 cut(s) 791, 905
MalI GATC 2 cut(s) 314, 1127
MboI GATC 2 cut(s) 312, 1125
MboII GAAGA 3 cut(s) 634, 755, 1244
MfeI CAATTG 2 cut(s) 50, 1340
MhlI GDGCHC 1 cut(s) 1234
Mly113I GGCGCC 1 cut(s) 1167
MlyI GAGTC 3 cut(s) 2, 267, 1070
MnlI CCTC 7 cut(s) 168, 618, 1078, 1180, 1194, 1197, 1234
MroNI GCCGGC 1 cut(s) 297
MslI CAYNNNNRTG 2 cut(s) 334, 1225
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 2 cut(s) 298, 1164
MspR9I CCNGG 2 cut(s) 1164, 1211
MunI CAATTG 2 cut(s) 50, 1340
MvaI CCWGG 1 cut(s) 1211
MvnI CGCG 1 cut(s) 1139
MwoI GCNNNNNNNGC 1 cut(s) 945
NaeI GCCGGC 1 cut(s) 299
NarI GGCGCC 1 cut(s) 1167
NciI CCSGG 1 cut(s) 1164
NdeII GATC 2 cut(s) 312, 1125
NgoMIV GCCGGC 1 cut(s) 297
NlaIII CATG 5 cut(s) 111, 328, 968, 1208, 1224
NlaIV GGNNCC 5 cut(s) 1151, 1168, 1181, 1182, 1233
NmuCI GTSAC 2 cut(s) 791, 905
NspI RCATGY 1 cut(s) 328
PacI TTAATTAA 2 cut(s) 660, 750
PaeI GCATGC 1 cut(s) 328
PdiI GCCGGC 1 cut(s) 299
PfeI GAWTC 2 cut(s) 724, 854
PflMI CCANNNNNTGG 1 cut(s) 293
PleI GAGTC 3 cut(s) 2, 266, 1070
PluTI GGCGCC 1 cut(s) 1170
PmaCI CACGTG 2 cut(s) 796, 944
PmlI CACGTG 2 cut(s) 796, 944
PpsI GAGTC 3 cut(s) 2, 266, 1070
Ppu21I YACGTR 2 cut(s) 796, 944
PpuMI RGGWCCY 1 cut(s) 1180
PshBI ATTAAT 2 cut(s) 746, 1299
PsiI TTATAA 2 cut(s) 72, 488
Psp1406I AACGTT 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 1180
Psp6I CCWGG 1 cut(s) 1209
PspCI CACGTG 2 cut(s) 796, 944
PspGI CCWGG 1 cut(s) 1209
PspN4I GGNNCC 5 cut(s) 1151, 1168, 1181, 1182, 1233
PspPI GGNCC 2 cut(s) 300, 1180
PspPPI RGGWCCY 1 cut(s) 1180
RigI GGCCGGCC 1 cut(s) 301
RsaI GTAC 3 cut(s) 231, 360, 1112
RsaNI GTAC 3 cut(s) 230, 359, 1111
RseI CAYNNNNRTG 2 cut(s) 334, 1225
Sau3AI GATC 2 cut(s) 312, 1125
Sau96I GGNCC 2 cut(s) 300, 1180
SchI GAGTC 3 cut(s) 2, 267, 1070
ScrFI CCNGG 2 cut(s) 1164, 1211
SduI GDGCHC 1 cut(s) 1234
SfoI GGCGCC 1 cut(s) 1168
SinI GGWCC 1 cut(s) 1180
SmiI ATTTAAAT 1 cut(s) 928
SmiMI CAYNNNNRTG 2 cut(s) 334, 1225
SmlI CTYRAG 1 cut(s) 1106
SmoI CTYRAG 1 cut(s) 1106
SphI GCATGC 1 cut(s) 328
SsiI CCGC 4 cut(s) 215, 322, 1071, 1137
SspDI GGCGCC 1 cut(s) 1166
SspI AATATT 2 cut(s) 33, 148
SspMI CTAG 3 cut(s) 209, 356, 636
StyD4I CCNGG 2 cut(s) 1162, 1209
StyI CCWWGG 1 cut(s) 292
SwaI ATTTAAAT 1 cut(s) 928
TaaI ACNGT 3 cut(s) 505, 1194, 1236
TaiI ACGT 4 cut(s) 354, 791, 798, 946
TaqI TCGA 2 cut(s) 1196, 1290
TfiI GAWTC 2 cut(s) 724, 854
TseFI GTSAC 2 cut(s) 791, 905
Tsp45I GTSAC 2 cut(s) 791, 905
TspDTI ATGAA 5 cut(s) 96, 180, 492, 602, 953
Van91I CCANNNNNTGG 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 1180
VspI ATTAAT 2 cut(s) 746, 1299
XapI RAATTY 4 cut(s) 442, 813, 923, 929
XceI RCATGY 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 203
XspI CTAG 3 cut(s) 209, 356, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.