pycom06g15650

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
20160486 .. 20161155
670 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGCTAGGGCGGGCCCCCGTTTTTTGCTTATTTCGCCTGTACTCATTTCGGCTGGGCACGAAGCTTCAACTATATATCAGCGTAAAAGTTTCAGTCAAGCTCCTCCTCATTTCCAAACCCTCTCCATTAAATTGCTCTCTATTTCTTGCTTTTCATGCTTGCTTGGATTTTGGGTTTTGTGAAGAGAGGATGAAGTGTAGCAGTGAGTCTTCTTCCAAACCGGACAGGAAGACAGTGGAGAGAAACAGAAGAATTCAAATGAAAAGTCTCTGCTTCAAGCTTGCTTCACTTGTCCCTCCCCAACACTTCAAAACCACCAACTCCAAGGGTTTGGTTATTGTAAAAGGAGGTAGAGATCAGAAACAGTTAATTAATATATATAAAGGCAAAACCATAAACGGGACGGAAATGAAAATGAAGATTGTGTATTGTGACAAGATTAGTTTCAAGGAAATGAATTGTGTGTGTCAGAGAGAGAGAGCAGAAGCAACATGTATGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

168

Amino Acids

19.16

Weight (kDa)

9.74

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 10
AcsI RAATTY 1 cut(s) 252
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 1 cut(s) 491
AgsI TTSAA 5 cut(s) 68, 257, 277, 310, 448
AluBI AGCT 3 cut(s) 64, 100, 280
AluI AGCT 3 cut(s) 64, 100, 280
Alw26I GTCTC 1 cut(s) 272
AoxI GGCC 1 cut(s) 12
ApaI GGGCCC 1 cut(s) 16
ApoI RAATTY 1 cut(s) 252
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AseI ATTAAT 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 12, 13
BaeGI GKGCMC 2 cut(s) 16, 59
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 201, 236
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 5
BmgT120I GGNCC 2 cut(s) 12, 13
BmiI GGNNCC 2 cut(s) 14, 15
BpiI GAAGAC 2 cut(s) 201, 236
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 1 cut(s) 195
BseLI CCNNNNNNNGG 1 cut(s) 399
BseRI GAGGAG 2 cut(s) 92, 95
BseSI GKGCMC 2 cut(s) 16, 59
BseYI CCCAGC 1 cut(s) 52
BshFI GGCC 1 cut(s) 14
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 2 cut(s) 278, 415
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 2 cut(s) 278, 415
BsnI GGCC 1 cut(s) 14
Bsp120I GGGCCC 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 16, 59
Bsp143I GATC 1 cut(s) 355
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 14
BspLI GGNNCC 2 cut(s) 14, 15
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 235, 366
Bst6I CTCTTC 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 12, 160, 282
BstF5I GGATG 1 cut(s) 195
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 2 cut(s) 33, 155
BstNSI RCATGY 1 cut(s) 495
BstSLI GKGCMC 2 cut(s) 16, 59
BstV2I GAAGAC 2 cut(s) 201, 236
BstXI CCANNNNNNTGG 1 cut(s) 331
BsuRI GGCC 1 cut(s) 14
BtsCI GGATG 1 cut(s) 195
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 2 cut(s) 208, 240
Cac8I GCNNGC 3 cut(s) 12, 160, 282
Cfr13I GGNCC 2 cut(s) 12, 13
Csp6I GTAC 1 cut(s) 40
CviAII CATG 2 cut(s) 155, 492
CviJI RGCY 5 cut(s) 14, 52, 64, 100, 280
CviKI_1 RGCY 5 cut(s) 14, 52, 64, 100, 280
CviQI GTAC 1 cut(s) 40
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 177
EarI CTCTTC 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 324
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 2 cut(s) 158, 495
FaiI YATR 9 cut(s) 73, 75, 156, 377, 379, 381, 395, 493, 497
FaqI GGGAC 2 cut(s) 278, 415
FatI CATG 2 cut(s) 154, 491
FauI CCCGC 1 cut(s) 3
FokI GGATG 1 cut(s) 202
FriOI GRGCYC 1 cut(s) 16
FspBI CTAG 1 cut(s) 5
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 221
Hin1II CATG 2 cut(s) 158, 495
HindIII AAGCTT 2 cut(s) 62, 278
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 360, 471
HpyCH4III ACNGT 2 cut(s) 235, 366
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 155
Hsp92II CATG 2 cut(s) 158, 495
Kzo9I GATC 1 cut(s) 355
LmnI GCTCC 1 cut(s) 105
LpnPI CCDG 4 cut(s) 38, 50, 211, 234
MaeI CTAG 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 431
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 6 cut(s) 194, 201, 204, 241, 261, 430
MhlI GDGCHC 2 cut(s) 16, 59
MluCI AATT 4 cut(s) 130, 252, 369, 457
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 6 cut(s) 113, 116, 130, 180, 306, 341
MseI TTAA 3 cut(s) 128, 368, 372
MspI CCGG 1 cut(s) 221
MwoI GCNNNNNNNGC 2 cut(s) 33, 155
NdeII GATC 1 cut(s) 355
NlaIII CATG 2 cut(s) 158, 495
NlaIV GGNNCC 2 cut(s) 14, 15
NmuCI GTSAC 1 cut(s) 431
NspI RCATGY 1 cut(s) 495
PacI TTAATTAA 1 cut(s) 372
PciI ACATGT 1 cut(s) 491
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PscI ACATGT 1 cut(s) 491
PshBI ATTAAT 1 cut(s) 372
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 14, 15
PspOMI GGGCCC 1 cut(s) 12
PspPI GGNCC 2 cut(s) 12, 13
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 3 cut(s) 128, 368, 372
Sau3AI GATC 1 cut(s) 355
Sau96I GGNCC 2 cut(s) 12, 13
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 2 cut(s) 16, 59
SetI ASST 4 cut(s) 66, 102, 282, 352
Sse9I AATT 4 cut(s) 130, 252, 369, 457
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 5
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 235, 366
TasI AATT 4 cut(s) 130, 252, 369, 457
TatI WGTACW 1 cut(s) 39
Tru1I TTAA 3 cut(s) 128, 368, 372
Tru9I TTAA 3 cut(s) 128, 368, 372
TscAI CASTG 2 cut(s) 208, 240
TseFI GTSAC 1 cut(s) 431
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 6 cut(s) 143, 206, 275, 425, 431, 470
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 2 cut(s) 208, 240
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 495
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.