pycom06g16950

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
21050585 .. 21052680
2096 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGGCTTCCAACATTGGGATAATGGACAGTGCTTACTTTGTTGGGAGGAATGAGATTTTGACGTGGATCAACAATCGCCTTCAGCTCAATCTCTCCCGCATTGAAGAGGCTGCATCTGGTGCTGTGCAATGTCAAATGATGGATATGACCTATCCTGGTGTTGTTCCAATGCACAAGGTCAATTTTGATGCGAAAACAGAATATGATATGATCCAGAATTATAAGGTTCTACAGGAAGTATTCAACAAGCTAAAAATTGAGAAGCCTCTTGAAGTCAATAGGCTTGTTAAAGGCAGGCCTTTGGACAACTTGGAATTCTTGCAATGGCTGAAACGTTACTGTGATTCCATAAATGGTGGCATTATGAATGAAAACTATAACCCTGTGGAGCGAAGGTGCAAGGGTGGGAAGGATCGATCCCAAAGGTGCTCAGAAAATCTCAAAATCTCTGCAAGCAAGCAATTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0000922 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009629 GO:0009630 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010005 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030496 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0055028 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

157

Amino Acids

18.05

Weight (kDa)

8.88

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 114 1.1e-10 Calponin homology (CH) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AciI CCGC 1 cut(s) 97
AclI AACGTT 1 cut(s) 334
AclWI GGATC 4 cut(s) 74, 205, 411, 420
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 3 cut(s) 104, 244, 272
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 1 cut(s) 154
AluBI AGCT 2 cut(s) 85, 250
AluI AGCT 2 cut(s) 85, 250
Alw21I GWGCWC 1 cut(s) 431
AlwI GGATC 4 cut(s) 74, 205, 411, 420
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 1 cut(s) 97
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 156
BfmI CTRYAG 1 cut(s) 230
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 156
BmsI GCATC 2 cut(s) 122, 178
Bsa29I ATCGAT 1 cut(s) 415
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse3DI GCAATG 2 cut(s) 134, 329
BseBI CCWGG 1 cut(s) 156
BseCI ATCGAT 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 2 cut(s) 134, 329
BseMII CTCAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 97
BshFI GGCC 1 cut(s) 298
BshVI ATCGAT 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 431
BslI CCNNNNNNNGG 1 cut(s) 15
BsnI GGCC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 4 cut(s) 66, 210, 412, 416
BspACI CCGC 1 cut(s) 97
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 1 cut(s) 443
BspDI ATCGAT 1 cut(s) 415
BspPI GGATC 4 cut(s) 74, 205, 411, 420
BsrDI GCAATG 2 cut(s) 134, 329
BssMI GATC 4 cut(s) 66, 210, 412, 416
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 2 cut(s) 29, 341
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 296, 454, 458
BstDEI CTNAG 1 cut(s) 430
BstKTI GATC 4 cut(s) 69, 213, 415, 419
BstMBI GATC 4 cut(s) 66, 210, 412, 416
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 415
BsuRI GGCC 1 cut(s) 298
BsuTUI ATCGAT 1 cut(s) 415
BtrI CACGTC 1 cut(s) 63
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 3 cut(s) 296, 454, 458
ClaI ATCGAT 1 cut(s) 415
CviJI RGCY 8 cut(s) 5, 85, 110, 250, 265, 283, 298, 328
CviKI_1 RGCY 8 cut(s) 5, 85, 110, 250, 265, 283, 298, 328
DdeI CTNAG 1 cut(s) 430
DpnI GATC 4 cut(s) 68, 212, 414, 418
DpnII GATC 4 cut(s) 66, 210, 412, 416
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 65
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 154
FaiI YATR 8 cut(s) 146, 204, 209, 222, 350, 365, 378, 469
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 111
HaeIII GGCC 1 cut(s) 298
HinfI GANTC 1 cut(s) 344
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 2 cut(s) 214, 269
HpyAV CCTTC 3 cut(s) 89, 387, 403
HpyCH4III ACNGT 2 cut(s) 29, 341
HpyCH4IV ACGT 2 cut(s) 62, 334
HpyCH4V TGCA 6 cut(s) 113, 127, 172, 322, 399, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 1 cut(s) 430
HpySE526I ACGT 2 cut(s) 62, 334
Kzo9I GATC 4 cut(s) 66, 210, 412, 416
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 7 cut(s) 102, 141, 168, 218, 227, 280, 396
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 2 cut(s) 122, 178
MaeII ACGT 2 cut(s) 62, 334
MaeIII GTNAC 1 cut(s) 335
MalI GATC 4 cut(s) 68, 212, 414, 418
MboI GATC 4 cut(s) 66, 210, 412, 416
MboII GAAGA 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 431
MluCI AATT 5 cut(s) 181, 217, 255, 314, 461
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 3 cut(s) 39, 100, 276
MseI TTAA 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 1 cut(s) 119
NdeII GATC 4 cut(s) 66, 210, 412, 416
PceI AGGCCT 1 cut(s) 298
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 1 cut(s) 112
PsiI TTATAA 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 334
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 4 cut(s) 66, 210, 412, 416
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 1 cut(s) 431
SetI ASST 9 cut(s) 65, 87, 152, 180, 228, 252, 337, 398, 428
SfaNI GCATC 2 cut(s) 122, 178
SfcI CTRYAG 1 cut(s) 230
Sse9I AATT 5 cut(s) 181, 217, 255, 314, 461
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 1 cut(s) 97
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 1 cut(s) 154
TaaI ACNGT 2 cut(s) 29, 341
TaiI ACGT 2 cut(s) 65, 337
TaqI TCGA 1 cut(s) 415
TasI AATT 5 cut(s) 181, 217, 255, 314, 461
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 34
TseI GCWGC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 380, 384
TspRI CASTG 1 cut(s) 34
XapI RAATTY 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.