pycom06g18490

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
22241896 .. 22243241
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGAAAGCGCCAGGATACGACACGTCCCTCTTCAAATTTCGTTCCTTCCATTCTTCTTCATTTCTTCTCAGTCCTCACAGTTGCGGTGAGCTGAGAGGGATGTCGAAGCTCATCATCCTCCACACTTCCTACCACCTCACCCCAATCCACCGCCGCGCCTGCAACTCCGTCGTTTCGCCCTCTCTCTTCACTCCCTCCTCCCTCCGCTCCCAATGCCTCCGTCCCCAATCCATCTCTCCCTCTCCCCCTCTTTCTCTGCGGAGCATTTCAGCTCGCGCCTTCGACTCGTCGTCGCCGGAGACCAAGGAAGAAGAATTGAAGGAGAGCGATGGCGAGATTGTAAATAAAACCACAACAGCAGACACCAAGGAATATCCGAGCGGCGAGTTTCAGTTCGAGAAGGCGAGCGCGTGGAAGAGCTTCGTCGTCAAGCTCCGCATGCTCATTGCCTTCCCTTGGCAGCGCGTCAAGAAAGGCAGCGTCTTCACCATCAAATTGCGCGGCCAGGTGTCTGATCAGCTGAAGAGTCGATTTTCGAGTGGACTTTCGCTGCCTCAAATTTGTGAGAATTTGATGAAAGCAGCGTACGATCCTCGAATTTCCGGTGTCTATCTCCAAATTGAAAGTTTGAATTGTGGCTGGGGCAAAGTCGAAGAAATCCGAAGACATATCTTGGATTTCAAGAAATCAGGTAAATTTGTTGTGGCCTATGTACCGGCTTGCGGAGAGAAAGAGTATTACCTTGCTAGTGCGTGCCAAGAGATATATGCACCTCCAAGTGCTTACTTTTCTTTGTTTGGGCTCACCGTTCAGTCCTCGTTCGTAAGAGGTGTTCTTGAGAAAGTTGGAATCGAGCCACAAGTGGAAAGGATTGGTAAATACAAAAGTGCCGGTCGATCAACTTGCGCGCACGACCATGTCCGAAGAGAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

312

Amino Acids

34.85

Weight (kDa)

9.42

Isoelectric Point (pI)

67.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 227 - 300 1.9e-12 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 207, 381
AccII CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
AciI CCGC 9 cut(s) 84, 151, 154, 205, 259, 381, 436, 501, 723
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 5 cut(s) 35, 558, 568, 597, 695
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 2 cut(s) 587, 714
AfiI CCNNNNNNNGG 2 cut(s) 456, 722
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 5 cut(s) 34, 319, 623, 631, 682
AhdI GACNNNNNGTC 1 cut(s) 289
AjiI CACGTC 1 cut(s) 24
AjnI CCWGG 2 cut(s) 10, 504
AluBI AGCT 6 cut(s) 91, 109, 272, 420, 433, 520
AluI AGCT 6 cut(s) 91, 109, 272, 420, 433, 520
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 584
AoxI GGCC 2 cut(s) 502, 705
ApeKI GCWGC 4 cut(s) 460, 477, 550, 581
ApoI RAATTY 5 cut(s) 35, 558, 568, 597, 695
Asp700I GAANNNNTTC 1 cut(s) 419
AspLEI GCGC 8 cut(s) 10, 158, 278, 410, 465, 501, 908, 910
AsuHPI GGTGA 4 cut(s) 98, 130, 478, 796
BanII GRGCYC 1 cut(s) 804
BbsI GAAGAC 2 cut(s) 475, 670
BbvI GCAGC 4 cut(s) 472, 489, 537, 593
BccI CCATC 3 cut(s) 239, 323, 497
BcgI CGANNNNNNTGC 4 cut(s) 151, 185, 885, 919
BciT130I CCWGG 2 cut(s) 12, 506
BciVI GTATCC 1 cut(s) 8
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 11
BfuI GTATCC 1 cut(s) 8
BisI GCNGC 7 cut(s) 154, 382, 461, 478, 502, 551, 582
BlsI GCNGC 7 cut(s) 155, 383, 462, 479, 503, 552, 583
Bme1390I CCNGG 2 cut(s) 12, 506
BmeRI GACNNNNNGTC 1 cut(s) 289
BmgBI CACGTC 1 cut(s) 24
BmrFI CCNGG 2 cut(s) 12, 506
BpiI GAAGAC 2 cut(s) 475, 670
BpuEI CTTGAG 1 cut(s) 857
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 303, 366, 455
BsaWI WCCGGW 1 cut(s) 602
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 722
Bse118I RCCGGY 2 cut(s) 715, 890
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 2 cut(s) 12, 506
BseDI CCNNGG 3 cut(s) 303, 366, 455
BseGI GGATG 2 cut(s) 105, 114
BseLI CCNNNNNNNGG 2 cut(s) 456, 722
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 82, 83
BsePI GCGCGC 1 cut(s) 906
BseRI GAGGAG 1 cut(s) 187
BseXI GCAGC 4 cut(s) 472, 489, 537, 593
BseYI CCCAGC 1 cut(s) 639
Bsh1236I CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
Bsh1285I CGRYCG 1 cut(s) 895
BshFI GGCC 2 cut(s) 504, 707
BsiEI CGRYCG 1 cut(s) 895
BsiSI CCGG 4 cut(s) 296, 603, 716, 891
BsiWI CGTACG 1 cut(s) 585
BslFI GGGAC 2 cut(s) 10, 207
BslI CCNNNNNNNGG 2 cut(s) 456, 722
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 2 cut(s) 10, 207
BsnI GGCC 2 cut(s) 504, 707
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 804
Bsp143I GATC 3 cut(s) 514, 589, 896
BspACI CCGC 9 cut(s) 84, 151, 154, 205, 259, 381, 436, 501, 723
BspANI GGCC 2 cut(s) 504, 707
BspCNI CTCAG 2 cut(s) 81, 84
BspFNI CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
BspPI GGATC 1 cut(s) 584
BspQI GCTCTTC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 293
BsrBI CCGCTC 2 cut(s) 207, 381
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 2 cut(s) 715, 890
BssAI RCCGGY 2 cut(s) 715, 890
BssECI CCNNGG 3 cut(s) 303, 366, 455
BssHII GCGCGC 1 cut(s) 906
BssMI GATC 3 cut(s) 514, 589, 896
BssT1I CCWWGG 3 cut(s) 303, 366, 455
Bst2UI CCWGG 2 cut(s) 12, 506
Bst4CI ACNGT 2 cut(s) 80, 808
Bst6I CTCTTC 5 cut(s) 35, 191, 410, 518, 919
BstC8I GCNNGC 7 cut(s) 160, 274, 406, 440, 721, 754, 908
BstDEI CTNAG 2 cut(s) 68, 92
BstF5I GGATG 2 cut(s) 105, 114
BstFNI CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 8 cut(s) 10, 158, 278, 410, 465, 501, 908, 910
BstKTI GATC 3 cut(s) 517, 592, 899
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 3 cut(s) 514, 589, 896
BstMCI CGRYCG 1 cut(s) 895
BstMWI GCNNNNNNNGC 3 cut(s) 159, 213, 439
BstNI CCWGG 2 cut(s) 12, 506
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 10, 504
BstUI CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
BstV1I GCAGC 4 cut(s) 472, 489, 537, 593
BstV2I GAAGAC 2 cut(s) 475, 670
BsuI GTATCC 1 cut(s) 8
BsuRI GGCC 2 cut(s) 504, 707
BtgZI GCGATG 1 cut(s) 342
BtrI CACGTC 1 cut(s) 24
BtsCI GGATG 2 cut(s) 105, 114
Cac8I GCNNGC 7 cut(s) 160, 274, 406, 440, 721, 754, 908
CfoI GCGC 8 cut(s) 10, 158, 278, 410, 465, 501, 908, 910
Cfr10I RCCGGY 2 cut(s) 715, 890
CseI GACGC 2 cut(s) 454, 469
Csp6I GTAC 2 cut(s) 586, 713
CviAII CATG 2 cut(s) 439, 917
CviQI GTAC 2 cut(s) 586, 713
DdeI CTNAG 2 cut(s) 68, 92
DpnI GATC 3 cut(s) 516, 591, 898
DpnII GATC 3 cut(s) 514, 589, 896
DriI GACNNNNNGTC 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 5 cut(s) 35, 191, 410, 518, 919
Eam1105I GACNNNNNGTC 1 cut(s) 289
EarI CTCTTC 5 cut(s) 35, 191, 410, 518, 919
Eco130I CCWWGG 3 cut(s) 303, 366, 455
Eco24I GRGCYC 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 10, 504
EcoT14I CCWWGG 3 cut(s) 303, 366, 455
EcoT38I GRGCYC 1 cut(s) 804
ErhI CCWWGG 3 cut(s) 303, 366, 455
FaeI CATG 2 cut(s) 442, 920
FaiI YATR 6 cut(s) 440, 669, 711, 766, 768, 918
FaqI GGGAC 2 cut(s) 10, 207
FatI CATG 2 cut(s) 438, 916
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 7 cut(s) 154, 382, 461, 478, 502, 551, 582
FokI GGATG 2 cut(s) 101, 112
FriOI GRGCYC 1 cut(s) 804
Fsp4HI GCNGC 7 cut(s) 154, 382, 461, 478, 502, 551, 582
FspBI CTAG 1 cut(s) 747
GlaI GCGC 8 cut(s) 9, 157, 277, 409, 464, 500, 907, 909
GluI GCNGC 7 cut(s) 154, 382, 461, 478, 502, 551, 582
GsaI CCCAGC 1 cut(s) 643
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 2 cut(s) 504, 707
HapII CCGG 4 cut(s) 296, 603, 716, 891
HgaI GACGC 2 cut(s) 454, 469
HhaI GCGC 8 cut(s) 10, 158, 278, 410, 465, 501, 908, 910
Hin1II CATG 2 cut(s) 442, 920
Hin6I GCGC 8 cut(s) 8, 156, 276, 408, 463, 499, 906, 908
HinP1I GCGC 8 cut(s) 8, 156, 276, 408, 463, 499, 906, 908
HinfI GANTC 3 cut(s) 284, 526, 849
HpaII CCGG 4 cut(s) 296, 603, 716, 891
HphI GGTGA 4 cut(s) 98, 130, 478, 796
Hpy166II GTNNAC 1 cut(s) 542
Hpy188I TCNGA 4 cut(s) 378, 514, 662, 923
Hpy188III TCNNGA 4 cut(s) 397, 469, 682, 836
Hpy8I GTNNAC 1 cut(s) 542
Hpy99I CGWCG 4 cut(s) 173, 292, 295, 428
HpyAV CCTTC 5 cut(s) 55, 289, 313, 394, 460
HpyCH4III ACNGT 2 cut(s) 80, 808
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 2 cut(s) 162, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 213, 439
HpyF3I CTNAG 2 cut(s) 68, 92
HpySE526I ACGT 1 cut(s) 23
Hsp92II CATG 2 cut(s) 442, 920
HspAI GCGC 8 cut(s) 8, 156, 276, 408, 463, 499, 906, 908
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 3 cut(s) 514, 589, 896
LguI GCTCTTC 1 cut(s) 410
LmnI GCTCC 3 cut(s) 212, 261, 438
Lsp1109I GCAGC 4 cut(s) 472, 489, 537, 593
MaeI CTAG 1 cut(s) 747
MaeII ACGT 1 cut(s) 23
MalI GATC 3 cut(s) 516, 591, 898
MbiI CCGCTC 2 cut(s) 207, 381
MboI GATC 3 cut(s) 514, 589, 896
MhlI GDGCHC 1 cut(s) 804
MlyI GAGTC 2 cut(s) 278, 535
MmeI TCCRAC 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 419
MslI CAYNNNNRTG 1 cut(s) 915
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 4 cut(s) 296, 603, 716, 891
MspR9I CCNGG 2 cut(s) 12, 506
MvaI CCWGG 2 cut(s) 12, 506
MvnI CGCG 6 cut(s) 156, 276, 410, 465, 501, 908
MwoI GCNNNNNNNGC 3 cut(s) 159, 213, 439
NdeII GATC 3 cut(s) 514, 589, 896
NlaIII CATG 2 cut(s) 442, 920
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PauI GCGCGC 1 cut(s) 906
PciSI GCTCTTC 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 849
Pfl23II CGTACG 1 cut(s) 585
PflFI GACNNNGTC 1 cut(s) 917
PkrI GCNGC 7 cut(s) 155, 383, 462, 479, 503, 552, 583
PleI GAGTC 2 cut(s) 278, 534
PpsI GAGTC 2 cut(s) 278, 534
Psp6I CCWGG 2 cut(s) 10, 504
PspFI CCCAGC 1 cut(s) 639
PspGI CCWGG 2 cut(s) 10, 504
PspLI CGTACG 1 cut(s) 585
PsrI GAACNNNNNNTAC 2 cut(s) 816, 848
PsyI GACNNNGTC 1 cut(s) 917
PteI GCGCGC 1 cut(s) 906
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 2 cut(s) 587, 714
RsaNI GTAC 2 cut(s) 586, 713
RseI CAYNNNNRTG 1 cut(s) 915
SapI GCTCTTC 1 cut(s) 410
SatI GCNGC 7 cut(s) 154, 382, 461, 478, 502, 551, 582
Sau3AI GATC 3 cut(s) 514, 589, 896
SchI GAGTC 2 cut(s) 278, 535
ScrFI CCNGG 2 cut(s) 12, 506
SduI GDGCHC 1 cut(s) 804
SmiMI CAYNNNNRTG 1 cut(s) 915
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
SphI GCATGC 1 cut(s) 442
SsiI CCGC 9 cut(s) 84, 151, 154, 205, 259, 381, 436, 501, 723
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 2 cut(s) 10, 504
StyI CCWWGG 3 cut(s) 303, 366, 455
TaaI ACNGT 2 cut(s) 80, 808
TaiI ACGT 1 cut(s) 26
TaqI TCGA 9 cut(s) 104, 282, 396, 529, 536, 595, 651, 852, 895
TauI GCSGC 3 cut(s) 156, 384, 504
TfiI GAWTC 1 cut(s) 849
TseI GCWGC 4 cut(s) 460, 477, 550, 581
TspDTI ATGAA 3 cut(s) 17, 48, 590
TspGWI ACGGA 2 cut(s) 157, 209
Tth111I GACNNNGTC 1 cut(s) 917
XapI RAATTY 5 cut(s) 35, 558, 568, 597, 695
XceI RCATGY 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.