pycom06g18660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Reverse (-)
22321762 .. 22322256
495 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGTATCATCAGATTTCTATCCAAACCTCAGGTCATGAGGGAGCAAGGCCTAGAAAACTCGATGCCGATTTCGCCATTATTGTAGCAAATTCATTGAAGCTAATGACACCATCTCCATCCGTATCAGCCTCTTTGATCATCTCCGTCAGCTCCTTATACGTCAACGGTTGTCCCATTTTCGCCATGGACCCTGCCAGCTCTGCCGCAGTTATGTACCCATTACCGTCACGATCAAAAGAGCGAAAAACCTCCAAGAGTTGCTCTTGGTTTACCAAAATCTCCTCGTTCATATCGGGCAATATGGCACCGACCAACTCGTCGAACTCCACGGAACCATTGCCGTTTGCGTCCATGTTGGCTAGGAGGACGTGGATTTGGTCCCCCGAGGGCTTAAGGCCGAGCGAGCGGAGGAGCGCCGCAAGCTCCAAGATGGTGAGGCTGCCGTCAGAATCCATGTCGAATCTGGCAAAGATGTCTCTTAGCTGGTTGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

17.86

Weight (kDa)

10.08

Isoelectric Point (pI)

68.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 304
AccBSI CCGCTC 1 cut(s) 406
AciI CCGC 3 cut(s) 204, 406, 417
AcsI RAATTY 1 cut(s) 88
AfaI GTAC 1 cut(s) 215
AflII CTTAAG 1 cut(s) 391
AgsI TTSAA 1 cut(s) 97
AjiI CACGTC 1 cut(s) 369
AluBI AGCT 5 cut(s) 100, 150, 198, 423, 483
AluI AGCT 5 cut(s) 100, 150, 198, 423, 483
Alw26I GTCTC 1 cut(s) 480
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 2 cut(s) 47, 395
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 1 cut(s) 88
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 187, 378
AsuHPI GGTGA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 2 cut(s) 187, 378
AxyI CCTNAGG 1 cut(s) 28
BanI GGYRCC 1 cut(s) 304
BbvI GCAGC 1 cut(s) 426
BccI CCATC 3 cut(s) 118, 124, 424
BceAI ACGGC 2 cut(s) 325, 427
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 2 cut(s) 51, 360
BfoI RGCGCY 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 391
BisI GCNGC 3 cut(s) 204, 417, 440
BlsI GCNGC 3 cut(s) 205, 418, 441
Bme18I GGWCC 2 cut(s) 187, 378
BmeT110I CYCGRG 1 cut(s) 383
BmgBI CACGTC 1 cut(s) 369
BmgT120I GGNCC 2 cut(s) 187, 378
BmiI GGNNCC 4 cut(s) 189, 306, 333, 380
BmsI GCATC 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 183, 327, 384
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bse21I CCTNAGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 335
Bse8I GATNNNNATC 1 cut(s) 17
BseDI CCNNGG 3 cut(s) 183, 327, 384
BseGI GGATG 1 cut(s) 116
BseJI GATNNNNATC 1 cut(s) 17
BseMI GCAATG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 42
BseRI GAGGAG 2 cut(s) 271, 424
BseXI GCAGC 1 cut(s) 426
BshFI GGCC 2 cut(s) 49, 397
BshNI GGYRCC 1 cut(s) 304
BsiHKCI CYCGRG 1 cut(s) 383
BslFI GGGAC 2 cut(s) 156, 364
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 2 cut(s) 156, 364
BsnI GGCC 2 cut(s) 49, 397
BsoBI CYCGRG 1 cut(s) 383
Bsp143I GATC 2 cut(s) 135, 230
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 3 cut(s) 204, 406, 417
BspANI GGCC 2 cut(s) 49, 397
BspCNI CTCAG 1 cut(s) 41
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 4 cut(s) 189, 306, 333, 380
BspT107I GGYRCC 1 cut(s) 304
BspTI CTTAAG 1 cut(s) 391
BsrBI CCGCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 335
BssECI CCNNGG 3 cut(s) 183, 327, 384
BssMI GATC 2 cut(s) 135, 230
BssT1I CCWWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 167, 225
BstAFI CTTAAG 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 196, 404, 421
BstDEI CTNAG 2 cut(s) 28, 479
BstDSI CCRYGG 2 cut(s) 183, 327
BstF5I GGATG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 2 cut(s) 138, 233
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 2 cut(s) 135, 230
BstMWI GCNNNNNNNGC 4 cut(s) 71, 200, 403, 420
BstV1I GCAGC 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 28
BsuRI GGCC 2 cut(s) 49, 397
BtgI CCRYGG 2 cut(s) 183, 327
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 1 cut(s) 116
Cac8I GCNNGC 3 cut(s) 196, 404, 421
CciI TCATGA 1 cut(s) 34
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 2 cut(s) 187, 378
CseI GACGC 1 cut(s) 336
Csp6I GTAC 1 cut(s) 214
CviAII CATG 4 cut(s) 35, 184, 352, 454
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 2 cut(s) 28, 479
DpnI GATC 2 cut(s) 137, 232
DpnII GATC 2 cut(s) 135, 230
DrdI GACNNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 187, 378
Eco81I CCTNAGG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 383
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 4 cut(s) 38, 187, 355, 457
FaiI YATR 8 cut(s) 36, 157, 185, 212, 290, 302, 353, 455
FaqI GGGAC 2 cut(s) 156, 364
FatI CATG 4 cut(s) 34, 183, 351, 453
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 204, 417, 440
FokI GGATG 1 cut(s) 103
Fsp4HI GCNGC 3 cut(s) 204, 417, 440
FspBI CTAG 2 cut(s) 51, 360
GlaI GCGC 1 cut(s) 415
GluI GCNGC 3 cut(s) 204, 417, 440
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 2 cut(s) 49, 397
HgaI GACGC 1 cut(s) 336
HhaI GCGC 1 cut(s) 416
Hin1II CATG 4 cut(s) 38, 187, 355, 457
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 449, 460
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 2 cut(s) 163, 270
Hpy188I TCNGA 2 cut(s) 12, 448
Hpy188III TCNNGA 2 cut(s) 35, 228
Hpy8I GTNNAC 2 cut(s) 163, 270
Hpy99I CGWCG 1 cut(s) 322
HpyCH4III ACNGT 2 cut(s) 167, 225
HpyCH4IV ACGT 2 cut(s) 159, 368
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 200, 403, 420
HpyF3I CTNAG 2 cut(s) 28, 479
HpySE526I ACGT 2 cut(s) 159, 368
Hsp92II CATG 4 cut(s) 38, 187, 355, 457
HspAI GCGC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 2 cut(s) 135, 230
LmnI GCTCC 4 cut(s) 41, 155, 411, 428
LpnPI CCDG 5 cut(s) 15, 204, 208, 449, 469
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 1 cut(s) 52
MaeI CTAG 2 cut(s) 51, 360
MaeII ACGT 2 cut(s) 159, 368
MaeIII GTNAC 1 cut(s) 225
MalI GATC 2 cut(s) 137, 232
MbiI CCGCTC 1 cut(s) 406
MboI GATC 2 cut(s) 135, 230
MluCI AATT 1 cut(s) 88
MnlI CCTC 9 cut(s) 31, 37, 139, 259, 292, 357, 379, 402, 429
MseI TTAA 1 cut(s) 392
MspCI CTTAAG 1 cut(s) 391
MwoI GCNNNNNNNGC 4 cut(s) 71, 200, 403, 420
NcoI CCATGG 1 cut(s) 183
NdeII GATC 2 cut(s) 135, 230
NlaIII CATG 4 cut(s) 38, 187, 355, 457
NlaIV GGNNCC 4 cut(s) 189, 306, 333, 380
NmeAIII GCCGAG 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 225
PagI TCATGA 1 cut(s) 34
PceI AGGCCT 1 cut(s) 49
PfeI GAWTC 2 cut(s) 449, 460
PkrI GCNGC 3 cut(s) 205, 418, 441
PspN4I GGNNCC 4 cut(s) 189, 306, 333, 380
PspPI GGNCC 2 cut(s) 187, 378
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 3 cut(s) 204, 417, 440
Sau3AI GATC 2 cut(s) 135, 230
Sau96I GGNCC 2 cut(s) 187, 378
SfaNI GCATC 1 cut(s) 52
SinI GGWCC 2 cut(s) 187, 378
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 1 cut(s) 88
SseBI AGGCCT 1 cut(s) 49
SsiI CCGC 3 cut(s) 204, 406, 417
SspMI CTAG 2 cut(s) 51, 360
StuI AGGCCT 1 cut(s) 49
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 167, 225
TaiI ACGT 2 cut(s) 162, 371
TaqI TCGA 3 cut(s) 60, 320, 458
TasI AATT 1 cut(s) 88
TauI GCSGC 2 cut(s) 206, 419
TfiI GAWTC 2 cut(s) 449, 460
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 2 cut(s) 81, 277
TspGWI ACGGA 3 cut(s) 109, 133, 344
Vha464I CTTAAG 1 cut(s) 391
VpaK11BI GGWCC 2 cut(s) 187, 378
XapI RAATTY 1 cut(s) 88
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 460
XspI CTAG 2 cut(s) 51, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.