pycom06g18910

Stabilization of polarity axis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
22476099 .. 22476602
504 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGATCTTGGGTGTAACCAACCTGTTTTTTCTTAAATCTCTTCGTAATATGCCCCATGTTGTTTCAGTTGGAAACCCTGCTCCTAATACAAACCGGCTTGCCCTTGCTTCCAGATCATCTACTGGAAGACTTACAGGTAGAGCTGAAGGATTTGGCTTTCAACAGCTTTCCTTGAAAAAATTCTCTCCTTCAAATTTGTTGAATGCTGTGAAGTTGAGGAGAGAGGGTCCTCTTTGTCTCATGACGGAACATAAGGAAGCCATTTGGAGCACTTACTCTGCTACAACTAAGCCGGACACTTCAATCCTAAATAGGCTTATAGATGCTGGGATGTCACCAAGGGTTGAGGAATCAATGTCAGTCGTTAACAATGAAATATTGCGGCGACATTTCTTGGAGCTCACCACTAACTTTTTGGCGCCTTTTGGCCCATATTTTAGGACTAGAACACCTTCAGAAGGATCGTTCTCCATTTGTAGACCCTCCCCCTCTTATTCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPA

Protein Analysis

168

Amino Acids

18.56

Weight (kDa)

10.27

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AccI GTMKAC 1 cut(s) 478
AciI CCGC 1 cut(s) 382
AclWI GGATC 1 cut(s) 469
AcsI RAATTY 2 cut(s) 179, 193
AcuI CTGAAG 2 cut(s) 165, 438
AcyI GRCGYC 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 5 cut(s) 161, 175, 192, 202, 303
AluBI AGCT 3 cut(s) 143, 166, 400
AluI AGCT 3 cut(s) 143, 166, 400
Alw21I GWGCWC 2 cut(s) 272, 402
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 1 cut(s) 469
AoxI GGCC 1 cut(s) 427
ApoI RAATTY 2 cut(s) 179, 193
Asp700I GAANNNNTTC 2 cut(s) 179, 451
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 2 cut(s) 227, 428
AsuHPI GGTGA 2 cut(s) 327, 394
AvaII GGWCC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 418
BanII GRGCYC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 2 cut(s) 272, 402
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 422
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 2 cut(s) 227, 428
BmiI GGNNCC 2 cut(s) 228, 420
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 93
Bse1I ACTGG 1 cut(s) 127
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 127
BseRI GAGGAG 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 326
BshFI GGCC 1 cut(s) 429
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 2 cut(s) 272, 402
BsiSI CCGG 2 cut(s) 94, 293
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 272, 402
Bsp143I GATC 3 cut(s) 3, 113, 461
BspACI CCGC 1 cut(s) 382
BspANI GGCC 1 cut(s) 429
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 228, 420
BspPI GGATC 1 cut(s) 469
BspT107I GGYRCC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 93
BsrI ACTGG 1 cut(s) 127
BssAI RCCGGY 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 3 cut(s) 3, 113, 461
BssNI GRCGYC 1 cut(s) 419
BssT1I CCWWGG 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 419
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 288
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 3 cut(s) 6, 116, 464
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 3 cut(s) 3, 113, 461
BstV2I GAAGAC 1 cut(s) 133
BsuRI GGCC 1 cut(s) 429
BtsCI GGATG 1 cut(s) 336
Cac8I GCNNGC 1 cut(s) 99
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 421
Cfr10I RCCGGY 1 cut(s) 93
Cfr13I GGNCC 2 cut(s) 227, 428
CviAII CATG 2 cut(s) 56, 241
CviJI RGCY 9 cut(s) 97, 143, 156, 166, 260, 292, 316, 400, 429
CviKI_1 RGCY 9 cut(s) 97, 143, 156, 166, 260, 292, 316, 400, 429
DdeI CTNAG 1 cut(s) 288
DinI GGCGCC 1 cut(s) 420
DpnI GATC 3 cut(s) 5, 115, 463
DpnII GATC 3 cut(s) 3, 113, 461
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 165, 438
EcoICRI GAGCTC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 402
EgeI GGCGCC 1 cut(s) 420
EheI GGCGCC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 338
FaeI CATG 2 cut(s) 59, 244
FaiI YATR 6 cut(s) 50, 57, 242, 252, 320, 433
FatI CATG 2 cut(s) 55, 240
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 1 cut(s) 343
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 444
GlaI GCGC 1 cut(s) 420
GluI GCNGC 1 cut(s) 383
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 1 cut(s) 429
HapII CCGG 2 cut(s) 94, 293
HhaI GCGC 1 cut(s) 421
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 2 cut(s) 59, 244
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 1 cut(s) 350
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 2 cut(s) 94, 293
HphI GGTGA 2 cut(s) 327, 394
Hpy166II GTNNAC 2 cut(s) 367, 479
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 2 cut(s) 111, 241
Hpy8I GTNNAC 2 cut(s) 367, 479
HpyAV CCTTC 4 cut(s) 140, 198, 452, 462
HpyF3I CTNAG 1 cut(s) 288
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 2 cut(s) 59, 244
HspAI GCGC 1 cut(s) 419
KasI GGCGCC 1 cut(s) 418
KspAI GTTAAC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 3, 113, 461
LmnI GCTCC 3 cut(s) 85, 267, 397
LpnPI CCDG 8 cut(s) 35, 90, 107, 108, 120, 124, 306, 312
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 13, 333
MalI GATC 3 cut(s) 5, 115, 463
MboI GATC 3 cut(s) 3, 113, 461
MboII GAAGA 2 cut(s) 32, 138
MhlI GDGCHC 2 cut(s) 272, 402
MluCI AATT 2 cut(s) 179, 193
Mly113I GGCGCC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 6 cut(s) 210, 217, 240, 340, 493, 499
MroXI GAANNNNTTC 2 cut(s) 179, 451
MseI TTAA 3 cut(s) 33, 366, 502
MspI CCGG 2 cut(s) 94, 293
Mva1269I GAATGC 1 cut(s) 208
NarI GGCGCC 1 cut(s) 419
NdeII GATC 3 cut(s) 3, 113, 461
NlaIII CATG 2 cut(s) 59, 244
NlaIV GGNNCC 2 cut(s) 228, 420
NmuCI GTSAC 1 cut(s) 333
PagI TCATGA 1 cut(s) 240
PctI GAATGC 1 cut(s) 208
PdmI GAANNNNTTC 2 cut(s) 179, 451
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 1 cut(s) 384
PluTI GGCGCC 1 cut(s) 422
PpuMI RGGWCCY 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 227
PspFI CCCAGC 1 cut(s) 326
PspN4I GGNNCC 2 cut(s) 228, 420
PspPI GGNCC 2 cut(s) 227, 428
PspPPI RGGWCCY 1 cut(s) 227
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 3 cut(s) 33, 366, 502
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 3 cut(s) 3, 113, 461
Sau96I GGNCC 2 cut(s) 227, 428
SduI GDGCHC 2 cut(s) 272, 402
SetI ASST 6 cut(s) 24, 139, 145, 168, 402, 454
SfaNI GCATC 1 cut(s) 313
SfoI GGCGCC 1 cut(s) 420
SinI GGWCC 1 cut(s) 227
Sse9I AATT 2 cut(s) 179, 193
SsiI CCGC 1 cut(s) 382
SspDI GGCGCC 1 cut(s) 418
SspI AATATT 1 cut(s) 378
SspMI CTAG 1 cut(s) 444
SstI GAGCTC 1 cut(s) 402
StyI CCWWGG 1 cut(s) 338
TasI AATT 2 cut(s) 179, 193
TauI GCSGC 1 cut(s) 385
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 3 cut(s) 33, 366, 502
Tru9I TTAA 3 cut(s) 33, 366, 502
TseFI GTSAC 1 cut(s) 333
Tsp45I GTSAC 1 cut(s) 333
TspDTI ATGAA 1 cut(s) 387
TspGWI ACGGA 2 cut(s) 260, 487
VpaK11BI GGWCC 1 cut(s) 227
XagI CCTNNNNNAGG 1 cut(s) 456
XapI RAATTY 2 cut(s) 179, 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 478
XmnI GAANNNNTTC 2 cut(s) 179, 451
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.