pycom06g18920

Stabilization of polarity axis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
N/A
Physical Location & Seq
Forward (+)
22476664 .. 22477871
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGAGATCTAACTGGCTGGACTTGTACCGGCGATTTTTACAGGGACCAAACTTTGCACCATGGTTTCAAAGAAGGCGTGCTGTTGCTGAACAAGAACAAGATAGACTGTGGAGGCACGCCAGAATGAAGACTGATATGCAGCAGTTGATGTCTAAGATGTCTGAATTGGAAATTATTGATTCCTTTAACTGTATTGAGAGGCATCTTCTTGAAGAAACACTGGCGCAGCAATCAGGAAGGGCTGGCGCAGACTCAACTGCAACCTGCCAAAAACTAAAGGGAGATCTGCTGGCAATTTTCAATGTACTTCCCAAGGACATGCAGCAACTTCTACTTTTAAACCCACAAAAGGCGGCTCTTCTAAAAGGAGCTCCTGAACATACAAAACTTCCCGGGCGCCCGCTTGCACAAGCTGGGTTTGTATCATCAACATCACCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPA

Protein Analysis

148

Amino Acids

16.85

Weight (kDa)

9.75

Isoelectric Point (pI)

63.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 396
AciI CCGC 2 cut(s) 353, 401
AcyI GRCGYC 1 cut(s) 397
AfaI GTAC 2 cut(s) 26, 306
AfiI CCNNNNNNNGG 1 cut(s) 349
AgsI TTSAA 3 cut(s) 68, 212, 301
AluBI AGCT 2 cut(s) 371, 413
AluI AGCT 2 cut(s) 371, 413
Alw21I GWGCWC 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 139, 226, 322
AspLEI GCGC 3 cut(s) 226, 248, 399
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 2 cut(s) 393, 394
AsuHPI GGTGA 1 cut(s) 426
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 44
BanI GGYRCC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 373
BbvI GCAGC 3 cut(s) 151, 238, 334
BcnI CCSGG 2 cut(s) 393, 394
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 272
BglII AGATCT 2 cut(s) 5, 283
BisI GCNGC 4 cut(s) 140, 227, 323, 354
BlsI GCNGC 4 cut(s) 141, 228, 324, 355
Bme1390I CCNGG 2 cut(s) 393, 394
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 45, 398
BmrFI CCNGG 2 cut(s) 393, 394
BmsI GCATC 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 134
BpuMI CCSGG 2 cut(s) 393, 394
BsaHI GRCGYC 1 cut(s) 397
BsaJI CCNNGG 3 cut(s) 59, 312, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 2 cut(s) 17, 225
BseDI CCNNGG 3 cut(s) 59, 312, 392
BseLI CCNNNNNNNGG 1 cut(s) 349
BseNI ACTGG 2 cut(s) 17, 225
BseXI GCAGC 3 cut(s) 151, 238, 334
BseYI CCCAGC 1 cut(s) 413
BshNI GGYRCC 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 2 cut(s) 28, 393
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 349
BsmFI GGGAC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 373
Bsp143I GATC 2 cut(s) 5, 283
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 2 cut(s) 353, 401
BspLI GGNNCC 2 cut(s) 45, 398
BspMI ACCTGC 1 cut(s) 272
BspQI GCTCTTC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 396
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 2 cut(s) 17, 225
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 3 cut(s) 59, 312, 392
BssMI GATC 2 cut(s) 5, 283
BssNI GRCGYC 1 cut(s) 397
BssT1I CCWWGG 2 cut(s) 59, 312
Bst4CI ACNGT 2 cut(s) 108, 191
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 1 cut(s) 397
BstC8I GCNNGC 6 cut(s) 78, 117, 244, 291, 401, 405
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 59
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 3 cut(s) 226, 248, 399
BstKTI GATC 2 cut(s) 8, 286
BstMBI GATC 2 cut(s) 5, 283
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 391, 392
BstV1I GCAGC 3 cut(s) 151, 238, 334
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 2 cut(s) 5, 283
BstYI RGATCY 2 cut(s) 5, 283
BtgI CCRYGG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 218
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 6 cut(s) 78, 117, 244, 291, 401, 405
CfoI GCGC 3 cut(s) 226, 248, 399
Cfr10I RCCGGY 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 44
Cfr9I CCCGGG 1 cut(s) 392
Csp6I GTAC 2 cut(s) 25, 305
CviAII CATG 2 cut(s) 60, 319
CviJI RGCY 5 cut(s) 16, 242, 356, 371, 413
CviKI_1 RGCY 5 cut(s) 16, 242, 356, 371, 413
CviQI GTAC 2 cut(s) 25, 305
DdeI CTNAG 1 cut(s) 153
DinI GGCGCC 1 cut(s) 398
DpnI GATC 2 cut(s) 7, 285
DpnII GATC 2 cut(s) 5, 283
DraI TTTAAA 1 cut(s) 339
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 2 cut(s) 59, 312
Eco24I GRGCYC 1 cut(s) 373
Eco47I GGWCC 1 cut(s) 44
Eco53kI GAGCTC 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 392
EcoICRI GAGCTC 1 cut(s) 371
EcoT14I CCWWGG 2 cut(s) 59, 312
EcoT38I GRGCYC 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 398
EheI GGCGCC 1 cut(s) 398
ErhI CCWWGG 2 cut(s) 59, 312
FaeI CATG 2 cut(s) 63, 322
FaiI YATR 4 cut(s) 61, 137, 320, 381
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 59, 318
FauI CCCGC 1 cut(s) 408
Fnu4HI GCNGC 4 cut(s) 140, 227, 323, 354
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 4 cut(s) 140, 227, 323, 354
GlaI GCGC 3 cut(s) 225, 247, 398
GluI GCNGC 4 cut(s) 140, 227, 323, 354
GsaI CCCAGC 1 cut(s) 417
HaeII RGCGCY 1 cut(s) 400
HapII CCGG 2 cut(s) 28, 393
HhaI GCGC 3 cut(s) 226, 248, 399
Hin1I GRCGYC 1 cut(s) 397
Hin1II CATG 2 cut(s) 63, 322
Hin6I GCGC 3 cut(s) 224, 246, 397
HinP1I GCGC 3 cut(s) 224, 246, 397
HinfI GANTC 2 cut(s) 179, 251
HpaII CCGG 2 cut(s) 28, 393
HphI GGTGA 1 cut(s) 426
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 3 cut(s) 209, 234, 374
HpyAV CCTTC 2 cut(s) 66, 231
HpyCH4III ACNGT 2 cut(s) 108, 191
HpyCH4V TGCA 5 cut(s) 56, 139, 260, 322, 407
HpyF3I CTNAG 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 397
Hsp92II CATG 2 cut(s) 63, 322
HspAI GCGC 3 cut(s) 224, 246, 397
KasI GGCGCC 1 cut(s) 396
Kzo9I GATC 2 cut(s) 5, 283
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 2 cut(s) 368, 376
Lsp1109I GCAGC 3 cut(s) 151, 238, 334
LweI GCATC 1 cut(s) 211
MalI GATC 2 cut(s) 7, 285
MboI GATC 2 cut(s) 5, 283
MboII GAAGA 4 cut(s) 139, 197, 224, 350
MflI RGATCY 2 cut(s) 5, 283
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 3 cut(s) 164, 171, 294
Mly113I GGCGCC 1 cut(s) 397
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 2 cut(s) 105, 192
MseI TTAA 2 cut(s) 186, 338
MspI CCGG 2 cut(s) 28, 393
MspR9I CCNGG 2 cut(s) 393, 394
NarI GGCGCC 1 cut(s) 397
NciI CCSGG 2 cut(s) 393, 394
NcoI CCATGG 1 cut(s) 59
NdeII GATC 2 cut(s) 5, 283
NlaIII CATG 2 cut(s) 63, 322
NlaIV GGNNCC 2 cut(s) 45, 398
NspI RCATGY 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 141, 228, 324, 355
PleI GAGTC 1 cut(s) 245
PluTI GGCGCC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 373
PspFI CCCAGC 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 45, 398
PspPI GGNCC 1 cut(s) 44
PsuI RGATCY 2 cut(s) 5, 283
RsaI GTAC 2 cut(s) 26, 306
RsaNI GTAC 2 cut(s) 25, 305
SacI GAGCTC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 186, 338
SatI GCNGC 4 cut(s) 140, 227, 323, 354
Sau3AI GATC 2 cut(s) 5, 283
Sau96I GGNCC 1 cut(s) 44
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 393, 394
SduI GDGCHC 1 cut(s) 373
SetI ASST 3 cut(s) 266, 373, 415
SfaNI GCATC 1 cut(s) 211
SfoI GGCGCC 1 cut(s) 398
SinI GGWCC 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 394
Sse9I AATT 3 cut(s) 164, 171, 294
SsiI CCGC 2 cut(s) 353, 401
SspDI GGCGCC 1 cut(s) 396
SstI GAGCTC 1 cut(s) 373
StyD4I CCNGG 2 cut(s) 391, 392
StyI CCWWGG 2 cut(s) 59, 312
TaaI ACNGT 2 cut(s) 108, 191
TasI AATT 3 cut(s) 164, 171, 294
TatI WGTACW 1 cut(s) 304
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 2 cut(s) 186, 338
Tru9I TTAA 2 cut(s) 186, 338
TscAI CASTG 1 cut(s) 225
TseI GCWGC 3 cut(s) 139, 226, 322
TspDTI ATGAA 1 cut(s) 140
TspMI CCCGGG 1 cut(s) 392
TspRI CASTG 1 cut(s) 225
VpaK11BI GGWCC 1 cut(s) 44
XceI RCATGY 1 cut(s) 322
XmaI CCCGGG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.