pycom07g00710

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Reverse (-)
608524 .. 610121
1598 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 738 bp
ATGAGTGATGAGATGATGAGAGTGTCATTCTTTCCCTCCCTGGAGAAGCTCAGGGTAGAATATTGCCCTGTTCTGAAGGGATGGTGGAGGGAGCACTCTCATAACAGTGCTGCTTCTTCATCAAAGCAAAATCTGTCATTCCCGCTCCCTTCGTTTCCTTGTCTTTCTGGATTGACGATCAGGCATTGTCCTATGATAAATTCCATGCCTCTGTATCCAAATGTGGATAGAATAGATCTCTATAGGACCAGCTCGAAGGTTCTTCCTTCCTTGTTTGTTAGAGGAGCGTCTGATATTACACATGATGTTGGTGTTGATGTTTCTGCCTCTTCTTCTTCCCCTCCTCTCTCCAAATTAACTTATATGTCACTTTATGGAATTGAGGATTTAGAATGTATAACGTCGGAAGGGATGTGCAACCTCACATCACTCCGATTGCTTTCAATTGAAAATTGCCCATATTTGGCATCAATACCGGAAGAAATAAGCAATCTCACTTCACTTCAGAAGCTTCAAATTGGATATTGCCCTAATTTGGCATCACTGCCAGAAGGGATTCGTCGACTCCCCTATTTAAGTACATTGAAAATTTGGGAGTGCCTCATGTTATGGTTCAGATGCAAGAAAGAAACAGTTAAGGTGCGTTTTCTAACTTGTTTCAATTTACATTTGGCTAATTCAGTAGTAGCTCAACTTACTCGTAAGCTCATGCAAGTTTGCTCATTCCATCAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

246

Amino Acids

27.75

Weight (kDa)

8.47

Isoelectric Point (pI)

50.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 114 - 200 2.6e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 562
AciI CCGC 1 cut(s) 143
AcsI RAATTY 2 cut(s) 199, 588
AcuI CTGAAG 2 cut(s) 95, 488
AfaI GTAC 1 cut(s) 580
AfiI CCNNNNNNNGG 2 cut(s) 463, 535
AgsI TTSAA 5 cut(s) 444, 449, 515, 586, 661
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 5 cut(s) 49, 252, 511, 689, 706
AluI AGCT 5 cut(s) 49, 252, 511, 689, 706
Alw21I GWGCWC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 2 cut(s) 199, 588
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 246
AvaII GGWCC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 97
BccI CCATC 2 cut(s) 75, 735
BciT130I CCWGG 1 cut(s) 41
BciVI GTATCC 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 241
BfuI GTATCC 1 cut(s) 225
BglII AGATCT 1 cut(s) 235
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 3 cut(s) 476, 548, 608
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 39
BsaWI WCCGGW 1 cut(s) 475
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 535
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 86, 417
BseLI CCNNNNNNNGG 2 cut(s) 463, 535
BseMII CTCAG 1 cut(s) 64
BseRI GAGGAG 2 cut(s) 297, 333
BseXI GCAGC 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 1 cut(s) 476
BslI CCNNNNNNNGG 2 cut(s) 463, 535
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 177, 235
BspACI CCGC 1 cut(s) 143
BspCNI CTCAG 1 cut(s) 63
BsrBI CCGCTC 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 177, 235
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 107, 634
Bst6I CTCTTC 1 cut(s) 334
BstDEI CTNAG 1 cut(s) 50
BstF5I GGATG 2 cut(s) 86, 417
BstKTI GATC 2 cut(s) 180, 238
BstMBI GATC 2 cut(s) 177, 235
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 39
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuI GTATCC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 86, 417
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 2 cut(s) 112, 542
Cfr13I GGNCC 1 cut(s) 246
CseI GACGC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 579
CviAII CATG 4 cut(s) 205, 302, 604, 709
CviJI RGCY 6 cut(s) 49, 252, 511, 674, 689, 706
CviKI_1 RGCY 6 cut(s) 49, 252, 511, 674, 689, 706
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 1 cut(s) 50
DpnI GATC 2 cut(s) 179, 237
DpnII GATC 2 cut(s) 177, 235
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 246
Eco57I CTGAAG 2 cut(s) 95, 488
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 4 cut(s) 208, 305, 607, 712
FatI CATG 4 cut(s) 204, 301, 603, 708
FauI CCCGC 1 cut(s) 150
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 1 cut(s) 111
FokI GGATG 2 cut(s) 93, 424
Fsp4HI GCNGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 111
GsuI CTGGAG 1 cut(s) 62
HapII CCGG 1 cut(s) 476
HgaI GACGC 1 cut(s) 276
Hin1II CATG 4 cut(s) 208, 305, 607, 712
HincII GTYRAC 1 cut(s) 563
HindII GTYRAC 1 cut(s) 563
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 2 cut(s) 556, 564
HpaII CCGG 1 cut(s) 476
Hpy166II GTNNAC 1 cut(s) 563
Hpy188I TCNGA 6 cut(s) 75, 292, 406, 434, 507, 617
Hpy188III TCNNGA 1 cut(s) 168
Hpy8I GTNNAC 1 cut(s) 563
Hpy99I CGWCG 2 cut(s) 406, 564
HpyAV CCTTC 6 cut(s) 70, 159, 250, 276, 401, 545
HpyCH4III ACNGT 2 cut(s) 107, 634
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 3 cut(s) 417, 621, 712
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 4 cut(s) 208, 305, 607, 712
Kzo9I GATC 2 cut(s) 177, 235
LmnI GCTCC 3 cut(s) 91, 150, 284
LpnPI CCDG 9 cut(s) 26, 37, 53, 81, 153, 166, 262, 489, 561
Lsp1109I GCAGC 1 cut(s) 97
LweI GCATC 3 cut(s) 476, 548, 608
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 366
MalI GATC 2 cut(s) 179, 237
MbiI CCGCTC 1 cut(s) 145
MboI GATC 2 cut(s) 177, 235
MboII GAAGA 6 cut(s) 108, 254, 321, 324, 327, 491
MfeI CAATTG 2 cut(s) 444, 731
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 1 cut(s) 384
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 3 cut(s) 356, 575, 636
MslI CAYNNNNRTG 1 cut(s) 105
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 1 cut(s) 41
MunI CAATTG 2 cut(s) 444, 731
MvaI CCWGG 1 cut(s) 41
NdeII GATC 2 cut(s) 177, 235
NlaIII CATG 4 cut(s) 208, 305, 607, 712
NmuCI GTSAC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 39
PspGI CCWGG 1 cut(s) 39
PspPI GGNCC 1 cut(s) 246
PsuI RGATCY 1 cut(s) 235
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
RseI CAYNNNNRTG 1 cut(s) 105
SalI GTCGAC 1 cut(s) 561
SaqAI TTAA 3 cut(s) 356, 575, 636
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 2 cut(s) 177, 235
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 96
SetI ASST 9 cut(s) 51, 254, 261, 404, 423, 513, 642, 691, 708
SfaNI GCATC 3 cut(s) 476, 548, 608
SfcI CTRYAG 1 cut(s) 241
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 105
SsiI CCGC 1 cut(s) 143
SspI AATATT 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 107, 634
TaiI ACGT 1 cut(s) 404
TaqI TCGA 2 cut(s) 254, 562
TatI WGTACW 1 cut(s) 578
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 3 cut(s) 356, 575, 636
Tru9I TTAA 3 cut(s) 356, 575, 636
TscAI CASTG 2 cut(s) 112, 549
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 1 cut(s) 108
TspRI CASTG 2 cut(s) 112, 549
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 2 cut(s) 199, 588
XmiI GTMKAC 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.