pycom07g06890

DNA replication ATP-dependent helicase nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Reverse (-)
6170138 .. 6170778
641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGACCGTCTTTAGCCATTTATCTCCACAAATTCATTTGGGCTCTCATAGAGGCCGGATCTCTATCATCTTCATCCCAAAAGCCCCACTACACCACTTTTCATTTTTCATTTTGGAGCCGTACGTTCATTTTGGCGGGAAAATCACAGATCAGCAGAAAGCGAAATCCAAAACCCCCAAATCTCCAATCGCACTCCGAGAACTGAACCAGCAGACCAGGAACTGGAAAAGCTACCACACCATTGCTCCAATGGCTCCGAGAAAGAAACCCATTTCTTCCAACACGACGTCGTCTAAGAAATCCAACTCGAAAGCTCAGCCGCAGCCCTCCAAGTTCGGCATCCAACATTTTTTTGAGCGCCACACCCAGAACTCTCAGAAACCCAAAGAGGTCGCCGCTTCTTCCCCTCATCAGAACCCTAAAACCCTAGCCGCCACCGTCTCTCCCACCGTTGCTCCAGTACATTCGGTTCCAAAGATCCGAAAGCCTGCTCAGATTGATGCGGGCAATGCGGTTTTAGCTTTGGTGAATGATGAAATTCAGTCGATATCGGAATTGGGGAATAAGAATAGCATGCCGAAGTACACGCCAGAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 map03030
Pfam Domains
Protein Families
AAA_11 AAA_12 Cas_Cas4 Dna2

Protein Analysis

200

Amino Acids

22.08

Weight (kDa)

10.27

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 222
AciI CCGC 6 cut(s) 135, 320, 396, 432, 503, 512
AclWI GGATC 2 cut(s) 65, 472
AcsI RAATTY 2 cut(s) 30, 537
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 3 cut(s) 122, 462, 584
AfiI CCNNNNNNNGG 1 cut(s) 222
AjnI CCWGG 1 cut(s) 215
AluBI AGCT 3 cut(s) 231, 314, 521
AluI AGCT 3 cut(s) 231, 314, 521
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 65, 472
AlwNI CAGNNNCTG 1 cut(s) 222
AoxI GGCC 1 cut(s) 52
ApeKI GCWGC 1 cut(s) 322
ApoI RAATTY 2 cut(s) 30, 537
AspLEI GCGC 1 cut(s) 360
AsuHPI GGTGA 1 cut(s) 538
BanII GRGCYC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 334
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 445
BfaI CTAG 1 cut(s) 428
BfoI RGCGCY 1 cut(s) 361
BisI GCNGC 4 cut(s) 320, 323, 396, 432
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 4 cut(s) 321, 324, 397, 433
Bme1390I CCNGG 1 cut(s) 217
BmiI GGNNCC 3 cut(s) 117, 255, 471
BmrFI CCNGG 1 cut(s) 217
BmsI GCATC 2 cut(s) 348, 490
BpmI CTGGAG 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 287
BsaXI ACNNNNNCTCC 6 cut(s) 229, 259, 427, 439, 457, 469
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 2 cut(s) 227, 458
Bse3DI GCAATG 2 cut(s) 240, 514
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 72, 339
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMI GCAATG 2 cut(s) 240, 514
BseMII CTCAG 3 cut(s) 329, 389, 506
BseNI ACTGG 2 cut(s) 227, 458
BseXI GCAGC 1 cut(s) 334
BshFI GGCC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 55
BsiWI CGTACG 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 445
BsmBI CGTCTC 1 cut(s) 445
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 3 cut(s) 57, 148, 477
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 6 cut(s) 135, 320, 396, 432, 503, 512
BspANI GGCC 1 cut(s) 54
BspCNI CTCAG 3 cut(s) 328, 388, 505
BspLI GGNNCC 3 cut(s) 117, 255, 471
BspPI GGATC 2 cut(s) 65, 472
BsrDI GCAATG 2 cut(s) 240, 514
BsrI ACTGG 2 cut(s) 227, 458
BssMI GATC 3 cut(s) 57, 148, 477
BssNI GRCGYC 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 3 cut(s) 7, 439, 451
BstACI GRCGYC 1 cut(s) 287
BstC8I GCNNGC 3 cut(s) 489, 505, 575
BstDEI CTNAG 4 cut(s) 294, 315, 375, 492
BstF5I GGATG 2 cut(s) 72, 339
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 3 cut(s) 60, 151, 480
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 3 cut(s) 57, 148, 477
BstMWI GCNNNNNNNGC 3 cut(s) 251, 509, 518
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 577
BstSCI CCNGG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 57, 477
BstYI RGATCY 2 cut(s) 57, 477
BsuRI GGCC 1 cut(s) 54
BtsCI GGATG 2 cut(s) 72, 339
Cac8I GCNNGC 3 cut(s) 489, 505, 575
CaiI CAGNNNCTG 1 cut(s) 222
CfoI GCGC 1 cut(s) 360
Csp6I GTAC 3 cut(s) 121, 461, 583
CviAII CATG 1 cut(s) 574
CviQI GTAC 3 cut(s) 121, 461, 583
DdeI CTNAG 4 cut(s) 294, 315, 375, 492
DpnI GATC 3 cut(s) 59, 150, 479
DpnII GATC 3 cut(s) 57, 148, 477
Eco24I GRGCYC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 215
EcoRV GATATC 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 44
Esp3I CGTCTC 1 cut(s) 445
FaeI CATG 1 cut(s) 577
FaiI YATR 2 cut(s) 48, 575
FatI CATG 1 cut(s) 573
FauI CCCGC 2 cut(s) 128, 496
Fnu4HI GCNGC 4 cut(s) 320, 323, 396, 432
FokI GGATG 2 cut(s) 59, 326
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 320, 323, 396, 432
FspBI CTAG 1 cut(s) 428
GlaI GCGC 1 cut(s) 359
GluI GCNGC 4 cut(s) 320, 323, 396, 432
GsuI CTGGAG 1 cut(s) 441
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 55
HhaI GCGC 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 1 cut(s) 577
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HpaII CCGG 1 cut(s) 55
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 1 cut(s) 585
Hpy188I TCNGA 7 cut(s) 197, 258, 378, 414, 482, 495, 553
Hpy8I GTNNAC 1 cut(s) 585
Hpy99I CGWCG 2 cut(s) 289, 292
HpyCH4III ACNGT 3 cut(s) 7, 439, 451
HpyCH4IV ACGT 2 cut(s) 123, 287
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 509, 518
HpyF3I CTNAG 4 cut(s) 294, 315, 375, 492
HpySE526I ACGT 2 cut(s) 123, 287
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 1 cut(s) 577
HspAI GCGC 1 cut(s) 358
Kzo9I GATC 3 cut(s) 57, 148, 477
LmnI GCTCC 4 cut(s) 115, 250, 259, 460
LpnPI CCDG 8 cut(s) 68, 202, 208, 221, 229, 380, 471, 501
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 2 cut(s) 348, 490
MaeI CTAG 1 cut(s) 428
MaeII ACGT 2 cut(s) 123, 287
MalI GATC 3 cut(s) 59, 150, 479
MboI GATC 3 cut(s) 57, 148, 477
MboII GAAGA 3 cut(s) 61, 267, 393
MflI RGATCY 2 cut(s) 57, 477
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 3 cut(s) 30, 537, 554
MmeI TCCRAC 3 cut(s) 303, 327, 367
MnlI CCTC 4 cut(s) 44, 337, 382, 417
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 251, 509, 518
NdeII GATC 3 cut(s) 57, 148, 477
NlaIII CATG 1 cut(s) 577
NlaIV GGNNCC 3 cut(s) 117, 255, 471
NspI RCATGY 1 cut(s) 577
PaeI GCATGC 1 cut(s) 577
Pfl23II CGTACG 1 cut(s) 120
PflFI GACNNNGTC 1 cut(s) 289
PflMI CCANNNNNTGG 1 cut(s) 222
PkrI GCNGC 4 cut(s) 321, 324, 397, 433
Psp6I CCWGG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 215
PspLI CGTACG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 117, 255, 471
PsrI GAACNNNNNNTAC 2 cut(s) 453, 485
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 2 cut(s) 57, 477
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 3 cut(s) 122, 462, 584
RsaNI GTAC 3 cut(s) 121, 461, 583
SatI GCNGC 4 cut(s) 320, 323, 396, 432
Sau3AI GATC 3 cut(s) 57, 148, 477
ScrFI CCNGG 1 cut(s) 217
SduI GDGCHC 1 cut(s) 44
SetI ASST 6 cut(s) 126, 233, 290, 316, 393, 523
SfaNI GCATC 2 cut(s) 348, 490
SphI GCATGC 1 cut(s) 577
Sse9I AATT 3 cut(s) 30, 537, 554
SsiI CCGC 6 cut(s) 135, 320, 396, 432, 503, 512
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 215
TaaI ACNGT 3 cut(s) 7, 439, 451
TaiI ACGT 2 cut(s) 126, 290
TaqI TCGA 2 cut(s) 308, 545
TasI AATT 3 cut(s) 30, 537, 554
TatI WGTACW 2 cut(s) 460, 582
TauI GCSGC 3 cut(s) 322, 398, 434
TseI GCWGC 1 cut(s) 322
TspDTI ATGAA 6 cut(s) 23, 61, 90, 97, 116, 549
Tth111I GACNNNGTC 1 cut(s) 289
Van91I CCANNNNNTGG 1 cut(s) 222
XapI RAATTY 2 cut(s) 30, 537
XceI RCATGY 1 cut(s) 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XspI CTAG 1 cut(s) 428
ZraI GACGTC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.