pycom07g07300

Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
6624786 .. 6627344
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
ATGGTGGATTTTGCGGATTTCCCAACCATCAATCTCTTTCTTGCAAAGTACTTCTATGACAATGATACATCGATACACCATGTTCAAACTCATTTCAAGACAGATGAGGACCCAAAGGAAGAGACAAATAAAACCTTCACAAACCCACCACTTGAACCCCAAAACCGAAACCAAAAAGAATTCGCGCTCCCTCACTCTACAATGGCGAACCTGAGGACGGCGATGGACTCAGCCTTCTGGGACTTGAACGTTTCTTCGCCTCAGACCCTTGAGGGCTCGGCCAAGGCCGTTCCCGGCGACCCCTTTCCTCTCGACGGTGCTCGAGCCAGCCGAGCCCTTAGGATTCAGCAACTCTGTCTTCTGGGGGAAATGGGTGTGATTCATTTGTTGAAAGATAATTTGTCTCCTCCTCTGCTTCTAGAAAGAGAGATTGACTTCTTTCGTCTCCAATTCCTTTTGCCAGACCTTGAATATACTTCCTTACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3769

Protein Analysis

163

Amino Acids

18.46

Weight (kDa)

4.89

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 185
AciI CCGC 1 cut(s) 14
AclI AACGTT 1 cut(s) 249
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 217, 314
AgsI TTSAA 6 cut(s) 86, 97, 155, 247, 391, 470
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 3 cut(s) 116, 408, 449
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 2 cut(s) 279, 285
ApoI RAATTY 1 cut(s) 179
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 294
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 1 cut(s) 109
AxyI CCTNAGG 2 cut(s) 212, 338
BanII GRGCYC 2 cut(s) 278, 337
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 322
BccI CCATC 2 cut(s) 35, 217
BceAI ACGGC 2 cut(s) 234, 272
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 3 cut(s) 116, 408, 449
BfaI CTAG 1 cut(s) 419
BmcAI AGTACT 1 cut(s) 50
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 350
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 282
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 314
Bse21I CCTNAGG 2 cut(s) 212, 338
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 217, 314
BseMII CTCAG 3 cut(s) 203, 243, 275
BseRI GAGGAG 2 cut(s) 396, 399
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 2 cut(s) 281, 287
BshVI ATCGAT 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 2 cut(s) 217, 314
BsmAI GTCTC 3 cut(s) 116, 408, 449
BsmBI CGTCTC 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 2 cut(s) 281, 287
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 3 cut(s) 278, 322, 337
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 281, 287
BspCNI CTCAG 3 cut(s) 204, 242, 274
BspDI ATCGAT 1 cut(s) 71
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 1 cut(s) 111
BssECI CCNNGG 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 282
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 4 cut(s) 212, 229, 261, 338
BstFNI CGCG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 187
BstMAI GTCTC 3 cut(s) 116, 408, 449
BstMWI GCNNNNNNNGC 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 292
BstUI CGCG 1 cut(s) 185
BstV2I GAAGAC 1 cut(s) 350
Bsu15I ATCGAT 1 cut(s) 71
Bsu36I CCTNAGG 2 cut(s) 212, 338
BsuRI GGCC 2 cut(s) 281, 287
BsuTUI ATCGAT 1 cut(s) 71
BtgZI GCGATG 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 328
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 109
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 1 cut(s) 49
CviAII CATG 2 cut(s) 80, 486
CviJI RGCY 7 cut(s) 233, 276, 281, 287, 326, 330, 335
CviKI_1 RGCY 7 cut(s) 233, 276, 281, 287, 326, 330, 335
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 4 cut(s) 212, 229, 261, 338
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 282
Eco24I GRGCYC 2 cut(s) 278, 337
Eco47I GGWCC 1 cut(s) 109
Eco81I CCTNAGG 2 cut(s) 212, 338
Eco88I CYCGRG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 282
EcoT38I GRGCYC 2 cut(s) 278, 337
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 2 cut(s) 83, 489
FaiI YATR 4 cut(s) 57, 81, 474, 487
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 79, 485
FriOI GRGCYC 2 cut(s) 278, 337
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 186
HaeIII GGCC 2 cut(s) 281, 287
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 187
Hin1II CATG 2 cut(s) 83, 489
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 3 cut(s) 227, 343, 379
HpaII CCGG 1 cut(s) 294
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 97, 311, 419
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 2 cut(s) 145, 244
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 1 cut(s) 44
HpyF10VI GCNNNNNNNGC 1 cut(s) 332
HpyF3I CTNAG 4 cut(s) 212, 229, 261, 338
HpySE526I ACGT 1 cut(s) 249
Hsp92II CATG 2 cut(s) 83, 489
HspAI GCGC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 192
LpnPI CCDG 6 cut(s) 223, 224, 307, 340, 347, 474
MaeI CTAG 1 cut(s) 419
MaeII ACGT 1 cut(s) 249
MboII GAAGA 3 cut(s) 131, 246, 350
MhlI GDGCHC 3 cut(s) 278, 322, 337
MluCI AATT 3 cut(s) 179, 397, 449
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 8 cut(s) 100, 201, 207, 265, 270, 318, 417, 420
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 332
NciI CCSGG 1 cut(s) 294
NlaIII CATG 2 cut(s) 83, 489
NlaIV GGNNCC 1 cut(s) 111
NmeAIII GCCGAG 2 cut(s) 257, 356
PaeR7I CTCGAG 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PfeI GAWTC 2 cut(s) 343, 379
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 109
Psp1406I AACGTT 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 109
PspPPI RGGWCCY 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 321
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
Sau96I GGNCC 1 cut(s) 109
ScaI AGTACT 1 cut(s) 50
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 3 cut(s) 278, 322, 337
SetI ASST 4 cut(s) 137, 213, 252, 468
Sfr274I CTCGAG 1 cut(s) 321
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 269, 321
SmoI CTYRAG 2 cut(s) 269, 321
Sse9I AATT 3 cut(s) 179, 397, 449
SsiI CCGC 1 cut(s) 14
SspMI CTAG 1 cut(s) 419
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 252
TaqI TCGA 3 cut(s) 71, 312, 322
TasI AATT 3 cut(s) 179, 397, 449
TatI WGTACW 1 cut(s) 48
TfiI GAWTC 2 cut(s) 343, 379
TspDTI ATGAA 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 179
XbaI TCTAGA 1 cut(s) 418
XhoI CTCGAG 1 cut(s) 321
XspI CTAG 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.