pycom07g12730

Golgin candidate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
14055776 .. 14056426
651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 492 bp
ATGCTGGGATTCTCTGACGAAGACAAGCAGAGAATAGGCGTCTCTCAAGGTGCAGGCAAAGGTGTTGTTCGTGGAGTTTTCTCTCTGCCTGGGCGCCTTGTTGGGGGAATTTTGGGAGGCAGCTCAGGTGGATTATCTGCAAATGCAGCTGCTGATAACCAGTCCTTTGCAGATCTGTGGGTTGATTTTCTTCTTAAGGAAACTAAAGAAAGGGAGAAAAGGGAGTTGGCAGACGGTTCAGGCAGATCTGATGAAGATTCATATAGAACTCCAAGCAAGGCACACACCGGTCAGGATCCCGACCACAGGACGACAACTAGCGGGTCTGAGCCTGGTTTCTCAAGAAGTAATTTATCACCAGTCCAAAACACAAGTTCCGCACCTTTTGGCAGTAATTTTCAGTCGGAGCATTCGGATTCCGAATTCTCAACAGTTCCTCTCACGTGGTCAGAAAGCAATCCATACGCCTCAAGAATGCTTCCTAGATTTTGA

Protein Analysis

164

Amino Acids

17.43

Weight (kDa)

5.36

Isoelectric Point (pI)

45.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 93
AciI CCGC 2 cut(s) 321, 378
AclWI GGATC 2 cut(s) 290, 303
AcsI RAATTY 2 cut(s) 108, 422
AcvI CACGTG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 39, 94
AfiI CCNNNNNNNGG 2 cut(s) 103, 306
AflII CTTAAG 1 cut(s) 194
AgeI ACCGGT 1 cut(s) 287
AjnI CCWGG 2 cut(s) 88, 331
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 2 cut(s) 123, 149
AluI AGCT 2 cut(s) 123, 149
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 2 cut(s) 290, 303
AlwNI CAGNNNCTG 1 cut(s) 152
ApeKI GCWGC 3 cut(s) 120, 146, 149
ApoI RAATTY 2 cut(s) 108, 422
AsiGI ACCGGT 1 cut(s) 287
AspLEI GCGC 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 348
BamHI GGATCC 1 cut(s) 295
BanI GGYRCC 1 cut(s) 93
BbrPI CACGTG 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 3 cut(s) 132, 136, 158
BciT130I CCWGG 2 cut(s) 90, 333
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 2 cut(s) 318, 483
BfoI RGCGCY 1 cut(s) 97
BfrI CTTAAG 1 cut(s) 194
BglII AGATCT 2 cut(s) 172, 245
BisI GCNGC 3 cut(s) 121, 147, 150
BlsI GCNGC 3 cut(s) 122, 148, 151
Bme1390I CCNGG 2 cut(s) 90, 333
BmiI GGNNCC 2 cut(s) 95, 297
BmrFI CCNGG 2 cut(s) 90, 333
BpiI GAAGAC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 124
BpuEI CTTGAG 3 cut(s) 30, 325, 454
BsaAI YACGTR 1 cut(s) 444
BsaHI GRCGYC 2 cut(s) 39, 94
BsaJI CCNNGG 1 cut(s) 89
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 306
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 160, 359
BseBI CCWGG 2 cut(s) 90, 333
BseDI CCNNGG 1 cut(s) 89
BseLI CCNNNNNNNGG 2 cut(s) 103, 306
BseMII CTCAG 2 cut(s) 138, 318
BseNI ACTGG 2 cut(s) 160, 359
BseXI GCAGC 3 cut(s) 132, 136, 158
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 93
BshTI ACCGGT 1 cut(s) 287
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 2 cut(s) 103, 306
BsmAI GTCTC 1 cut(s) 46
BsmBI CGTCTC 1 cut(s) 46
BsmI GAATGC 2 cut(s) 409, 480
Bsp143I GATC 3 cut(s) 172, 245, 295
BspACI CCGC 2 cut(s) 321, 378
BspCNI CTCAG 2 cut(s) 137, 319
BspLI GGNNCC 2 cut(s) 95, 297
BspPI GGATC 2 cut(s) 290, 303
BspT107I GGYRCC 1 cut(s) 93
BspTI CTTAAG 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 160, 359
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 89
BssMI GATC 3 cut(s) 172, 245, 295
BssNI GRCGYC 2 cut(s) 39, 94
Bst2UI CCWGG 2 cut(s) 90, 333
Bst4CI ACNGT 2 cut(s) 236, 433
BstACI GRCGYC 2 cut(s) 39, 94
BstAFI CTTAAG 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 444
BstC8I GCNNGC 1 cut(s) 55
BstDEI CTNAG 2 cut(s) 124, 327
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 3 cut(s) 175, 248, 298
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 3 cut(s) 172, 245, 295
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 2 cut(s) 90, 333
BstSCI CCNGG 2 cut(s) 88, 331
BstV1I GCAGC 3 cut(s) 132, 136, 158
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 3 cut(s) 172, 245, 295
BstYI RGATCY 3 cut(s) 172, 245, 295
Cac8I GCNNGC 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 1 cut(s) 96
Cfr10I RCCGGY 1 cut(s) 287
CseI GACGC 1 cut(s) 28
CspAI ACCGGT 1 cut(s) 287
CviJI RGCY 3 cut(s) 123, 149, 331
CviKI_1 RGCY 3 cut(s) 123, 149, 331
DdeI CTNAG 2 cut(s) 124, 327
DinI GGCGCC 1 cut(s) 95
DpnI GATC 3 cut(s) 174, 247, 297
DpnII GATC 3 cut(s) 172, 245, 295
Eco72I CACGTG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 88, 331
EgeI GGCGCC 1 cut(s) 95
EheI GGCGCC 1 cut(s) 95
Esp3I CGTCTC 1 cut(s) 46
FaiI YATR 3 cut(s) 262, 264, 463
FauI CCCGC 1 cut(s) 314
Fnu4HI GCNGC 3 cut(s) 121, 147, 150
Fsp4HI GCNGC 3 cut(s) 121, 147, 150
FspBI CTAG 2 cut(s) 318, 483
GlaI GCGC 1 cut(s) 95
GluI GCNGC 3 cut(s) 121, 147, 150
GsaI CCCAGC 1 cut(s) 8
HaeII RGCGCY 1 cut(s) 97
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 96
Hin1I GRCGYC 2 cut(s) 39, 94
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HinfI GANTC 3 cut(s) 9, 257, 416
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 348
Hpy188I TCNGA 7 cut(s) 16, 250, 328, 406, 415, 421, 451
Hpy188III TCNNGA 4 cut(s) 293, 299, 342, 471
HpyCH4III ACNGT 2 cut(s) 236, 433
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 4 cut(s) 53, 140, 146, 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 2 cut(s) 124, 327
HpySE526I ACGT 1 cut(s) 443
Hsp92I GRCGYC 2 cut(s) 39, 94
HspAI GCGC 1 cut(s) 94
KasI GGCGCC 1 cut(s) 93
Kzo9I GATC 3 cut(s) 172, 245, 295
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 3 cut(s) 132, 136, 158
MaeI CTAG 2 cut(s) 318, 483
MaeII ACGT 1 cut(s) 443
MalI GATC 3 cut(s) 174, 247, 297
MboI GATC 3 cut(s) 172, 245, 295
MboII GAAGA 3 cut(s) 32, 182, 266
MflI RGATCY 3 cut(s) 172, 245, 295
MluCI AATT 4 cut(s) 108, 349, 394, 422
Mly113I GGCGCC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 3 cut(s) 110, 447, 478
MseI TTAA 1 cut(s) 195
MspA1I CMGCKG 1 cut(s) 149
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 90, 333
Mva1269I GAATGC 2 cut(s) 409, 480
MvaI CCWGG 2 cut(s) 90, 333
MwoI GCNNNNNNNGC 1 cut(s) 146
NarI GGCGCC 1 cut(s) 94
NdeII GATC 3 cut(s) 172, 245, 295
NlaIV GGNNCC 2 cut(s) 95, 297
PctI GAATGC 2 cut(s) 409, 480
PfeI GAWTC 3 cut(s) 9, 257, 416
PinAI ACCGGT 1 cut(s) 287
PkrI GCNGC 3 cut(s) 122, 148, 151
PluTI GGCGCC 1 cut(s) 97
PmaCI CACGTG 1 cut(s) 444
PmlI CACGTG 1 cut(s) 444
Ppu21I YACGTR 1 cut(s) 444
Psp6I CCWGG 2 cut(s) 88, 331
PspCI CACGTG 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 88, 331
PspN4I GGNNCC 2 cut(s) 95, 297
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 3 cut(s) 172, 245, 295
PvuII CAGCTG 1 cut(s) 149
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 121, 147, 150
Sau3AI GATC 3 cut(s) 172, 245, 295
ScrFI CCNGG 2 cut(s) 90, 333
SetI ASST 7 cut(s) 52, 64, 125, 130, 151, 385, 446
SfoI GGCGCC 1 cut(s) 95
SmlI CTYRAG 4 cut(s) 45, 194, 340, 469
SmoI CTYRAG 4 cut(s) 45, 194, 340, 469
Sse9I AATT 4 cut(s) 108, 349, 394, 422
SsiI CCGC 2 cut(s) 321, 378
SspDI GGCGCC 1 cut(s) 93
SspMI CTAG 2 cut(s) 318, 483
StyD4I CCNGG 2 cut(s) 88, 331
TaaI ACNGT 2 cut(s) 236, 433
TaiI ACGT 1 cut(s) 446
TasI AATT 4 cut(s) 108, 349, 394, 422
TfiI GAWTC 3 cut(s) 9, 257, 416
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseI GCWGC 3 cut(s) 120, 146, 149
TspDTI ATGAA 2 cut(s) 249, 267
Vha464I CTTAAG 1 cut(s) 194
XapI RAATTY 2 cut(s) 108, 422
XspI CTAG 2 cut(s) 318, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.