pycom07g16280

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Reverse (-)
18721522 .. 18722016
495 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 363 bp
ATGCAAGCAGATGGCAAGAAGATTGTTGACCCCAACAAGCAAAATGCTTTATGTTCCCGCTTGAGAATTGAGCTCTTGCGTCCTCTTCGAGTGGCTGGGGTGAGCCGTGGTCCAGACACTGAGCTGCTGGTTGCTAACCCCGTTGAGTTGTCTGGCAGGGGTCATCCTCTTGTCTTTTATGATATCACACTGGCCCTCGAAGTTCTACAAACATGCATCTTTGCGGTTAAGATAGGCAGACACATGAACCGAGATCGAGAGTGGGAGGTATACAGGATCCTACTAGATGAAGGAGTAGGTTCTCTAGTGCCAAGGAACAAAATTGAGGAAGGTGTAAGAAACAAATTGATGGGTTGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.6

Weight (kDa)

8.68

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACT_ACR9_3rd PF24931 1 - 39 1.1e-12 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 40 - 118 1.1e-35 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 270
AciI CCGC 2 cut(s) 58, 224
AclWI GGATC 2 cut(s) 271, 284
AluBI AGCT 2 cut(s) 73, 124
AluI AGCT 2 cut(s) 73, 124
Alw21I GWGCWC 1 cut(s) 75
AlwI GGATC 2 cut(s) 271, 284
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 124
AspS9I GGNCC 2 cut(s) 110, 193
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 110
BamHI GGATCC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 1 cut(s) 111
BccI CCATC 2 cut(s) 5, 343
BceAI ACGGC 1 cut(s) 90
BfaI CTAG 2 cut(s) 284, 305
BisI GCNGC 1 cut(s) 125
BlsI GCNGC 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 2 cut(s) 110, 193
BmiI GGNNCC 1 cut(s) 278
BmsI GCATC 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 82
BsaJI CCNNGG 2 cut(s) 106, 311
Bse1I ACTGG 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 106, 311
BseGI GGATG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 95
BshFI GGCC 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 75
BsnI GGCC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 253, 276
BspACI CCGC 2 cut(s) 58, 224
BspANI GGCC 1 cut(s) 194
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 278
BspPI GGATC 2 cut(s) 271, 284
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 106, 311
BssMI GATC 2 cut(s) 253, 276
BssNAI GTATAC 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 311
Bst1107I GTATAC 1 cut(s) 271
Bst6I CTCTTC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 2 cut(s) 256, 279
BstMBI GATC 2 cut(s) 253, 276
BstNSI RCATGY 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
BstZ17I GTATAC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 2 cut(s) 117, 188
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 110, 193
CseI GACGC 1 cut(s) 68
CviAII CATG 2 cut(s) 213, 244
CviJI RGCY 5 cut(s) 73, 95, 105, 124, 194
CviKI_1 RGCY 5 cut(s) 73, 95, 105, 124, 194
DdeI CTNAG 1 cut(s) 120
DpnI GATC 2 cut(s) 255, 278
DpnII GATC 2 cut(s) 253, 276
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 75
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 73
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 311
EcoT22I ATGCAT 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 2 cut(s) 216, 247
FaiI YATR 5 cut(s) 52, 180, 214, 245, 271
FatI CATG 2 cut(s) 212, 243
FauI CCCGC 1 cut(s) 65
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 1 cut(s) 125
FokI GGATG 1 cut(s) 150
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 125
FspBI CTAG 2 cut(s) 284, 305
GluI GCNGC 1 cut(s) 125
GsaI CCCAGC 1 cut(s) 99
HaeIII GGCC 1 cut(s) 194
HgaI GACGC 1 cut(s) 68
Hin1II CATG 2 cut(s) 216, 247
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 2 cut(s) 28, 271
Hpy188III TCNNGA 2 cut(s) 113, 257
Hpy8I GTNNAC 2 cut(s) 28, 271
HpyAV CCTTC 2 cut(s) 284, 323
HpyCH4V TGCA 2 cut(s) 4, 216
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 2 cut(s) 216, 247
Kzo9I GATC 2 cut(s) 253, 276
LpnPI CCDG 7 cut(s) 81, 113, 126, 138, 142, 176, 259
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 1 cut(s) 225
MaeI CTAG 2 cut(s) 284, 305
MalI GATC 2 cut(s) 255, 278
MboI GATC 2 cut(s) 253, 276
MboII GAAGA 2 cut(s) 31, 77
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 66, 321, 344
MnlI CCTC 5 cut(s) 93, 177, 206, 259, 319
Mph1103I ATGCAT 1 cut(s) 218
MseI TTAA 1 cut(s) 228
NdeII GATC 2 cut(s) 253, 276
NlaIII CATG 2 cut(s) 216, 247
NlaIV GGNNCC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 218
NspI RCATGY 1 cut(s) 216
PkrI GCNGC 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 278
PspPI GGNCC 2 cut(s) 110, 193
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 276
SacI GAGCTC 1 cut(s) 75
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 1 cut(s) 125
Sau3AI GATC 2 cut(s) 253, 276
Sau96I GGNCC 2 cut(s) 110, 193
SduI GDGCHC 1 cut(s) 75
SetI ASST 5 cut(s) 75, 126, 270, 301, 334
SfaNI GCATC 1 cut(s) 225
SinI GGWCC 1 cut(s) 110
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 3 cut(s) 66, 321, 344
SsiI CCGC 2 cut(s) 58, 224
SspMI CTAG 2 cut(s) 284, 305
SstI GAGCTC 1 cut(s) 75
StyI CCWWGG 1 cut(s) 311
TaqI TCGA 3 cut(s) 88, 198, 256
TasI AATT 3 cut(s) 66, 321, 344
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 124, 195
TseI GCWGC 1 cut(s) 124
TspDTI ATGAA 2 cut(s) 260, 303
TspRI CASTG 2 cut(s) 124, 195
VpaK11BI GGWCC 1 cut(s) 110
XceI RCATGY 1 cut(s) 216
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 284, 305
Zsp2I ATGCAT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.