pycom07g17800

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
20346003 .. 20347387
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
ATGGGGATCCTGATGGCAACGTGTTTGCTGGCTCTTTTCTATGGTTTCTCCTGCCTCTGTAAGTTACTTCTCAGAAGGAGAGACCAGGCATGCTATTTGCTGGCCTACGAGTGCCACATGCCACCAGACGACATGAAGCTCAGCACTGATTCATGCGTTAAAATCGTCCTGCGGAACAGGAATTTGGCACTGGAAGAATTCAGGTTTCTCTTGAAAACTATTACCCATTCGGGCATTGGCGAGGAAACTTACTGCGCAAGAAACATCATCCAAGGCCGAGAAGAAACTGCAACCCTAGAAGATGAACTTGTGGAGATGGATGGCATCATCTTTGACGCGCTGGACAAGCTTTTCGCTCGGGCTACCTCAATTTCTCCGTCGCAAATCGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCCTCCCTATCGTCACGTATAGTGAATCGTTACAAGATGAGGGACGACATCAAGAGCTTCAACCTCTCGGGAATGGGCTGCAGTAACCTCCAAGTTCTAGCACCAAAAATGCTTCCACTAAGAGAAATACTTAGGTATCTGGTGGCCTCTAGGCTTAACAATATTAGAACTAGTATTACAAGCCAAAAGCCAAAAGCAGCTGGGGGACTAGGGTTGAATCTCAAGACAGGAATAGAGCACTTCTGTGTTCACCCTGGTGGAAGAGCAATCATAGATGGGATTGGGAAGAGCTTAGGGCTAAGTGATTATGACGTTGAGCCGTCAAGAATGGCGCTTCACCGGTTCGGAAACACATCGGCGGCCGGATTTTGGTACGCTTTGGGATACATGGAGGCAAAGAAGAGGCTTAAGAAGGGGAACAGGATTCTGATGAGTGGATTTGGAGCTGGTTTTAAGTGCAACAATATTGTGTGGGAAGTGATGAAGGACTTGGATGATGTCAATGTGTGGAAAGACTGCATAGATAGTTACCCTAGGGATCTAAACCAAGTCAATCCGCTTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

331

Amino Acids

36.91

Weight (kDa)

8.66

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 170 2.5e-52 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 219 - 301 7.1e-13 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 223 - 293 4.5e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
Acc16I TGCGCA 1 cut(s) 256
AccII CGCG 1 cut(s) 338
AciI CCGC 3 cut(s) 172, 785, 983
AclWI GGATC 2 cut(s) 14, 972
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 2 cut(s) 181, 197
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 1 cut(s) 795
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 20
AgeI ACCGGT 1 cut(s) 765
AgsI TTSAA 3 cut(s) 214, 487, 643
AhlI ACTAGT 1 cut(s) 596
AjnI CCWGG 2 cut(s) 84, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AleI CACNNNNGTG 2 cut(s) 669, 681
AluBI AGCT 6 cut(s) 139, 349, 483, 626, 717, 872
AluI AGCT 6 cut(s) 139, 349, 483, 626, 717, 872
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 2 cut(s) 14, 972
Ama87I CYCGRG 2 cut(s) 357, 493
AoxI GGCC 4 cut(s) 102, 274, 570, 786
ApeKI GCWGC 2 cut(s) 504, 623
ApoI RAATTY 2 cut(s) 181, 197
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 765
AspA2I CCTAGG 1 cut(s) 959
AspLEI GCGC 3 cut(s) 257, 340, 760
AsuHPI GGTGA 2 cut(s) 668, 755
AvaI CYCGRG 2 cut(s) 357, 493
AvrII CCTAGG 1 cut(s) 959
BaeI ACNNNNGTAYC 2 cut(s) 545, 578
BamHI GGATCC 1 cut(s) 6
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 491, 635
BccI CCATC 4 cut(s) 7, 310, 314, 695
BceAI ACGGC 1 cut(s) 730
BciT130I CCWGG 2 cut(s) 86, 681
BciVI GTATCC 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 75
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 6 cut(s) 296, 524, 576, 597, 635, 960
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 761
BfrI CTTAAG 1 cut(s) 833
BfuI GTATCC 1 cut(s) 803
BisI GCNGC 3 cut(s) 505, 624, 786
BlnI CCTAGG 1 cut(s) 959
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 3 cut(s) 506, 625, 787
Bme1390I CCNGG 2 cut(s) 86, 681
BmeT110I CYCGRG 2 cut(s) 357, 493
BmiI GGNNCC 1 cut(s) 8
BmrFI CCNGG 2 cut(s) 86, 681
BmsI GCATC 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 718
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 632
BsaAI YACGTR 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 271, 679, 959
BsaWI WCCGGW 1 cut(s) 765
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse118I RCCGGY 1 cut(s) 765
Bse1I ACTGG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 86, 681
BseDI CCNNGG 3 cut(s) 271, 679, 959
BseGI GGATG 3 cut(s) 267, 325, 925
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMII CTCAG 2 cut(s) 85, 154
BseNI ACTGG 1 cut(s) 195
BseX3I CGGCCG 1 cut(s) 786
BseXI GCAGC 2 cut(s) 491, 635
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 789
BshFI GGCC 4 cut(s) 104, 276, 572, 788
BshTI ACCGGT 1 cut(s) 765
BsiEI CGRYCG 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 666
BsiHKCI CYCGRG 2 cut(s) 357, 493
BsiSI CCGG 2 cut(s) 766, 789
BslFI GGGAC 2 cut(s) 482, 645
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 2 cut(s) 482, 645
BsnI GGCC 4 cut(s) 104, 276, 572, 788
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 2 cut(s) 357, 493
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 2 cut(s) 6, 964
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 3 cut(s) 172, 785, 983
BspANI GGCC 4 cut(s) 104, 276, 572, 788
BspCNI CTCAG 2 cut(s) 84, 153
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 1 cut(s) 8
BspMAI CTGCAG 1 cut(s) 509
BspPI GGATC 2 cut(s) 14, 972
BspQI GCTCTTC 2 cut(s) 682, 707
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 765
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 765
BssECI CCNNGG 3 cut(s) 271, 679, 959
BssMI GATC 2 cut(s) 6, 964
BssT1I CCWWGG 2 cut(s) 271, 959
Bst2UI CCWGG 2 cut(s) 86, 681
Bst6I CTCTTC 3 cut(s) 682, 707, 821
BstAFI CTTAAG 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 3 cut(s) 30, 91, 102
BstDEI CTNAG 6 cut(s) 71, 140, 545, 557, 718, 725
BstF5I GGATG 3 cut(s) 267, 325, 925
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 761
BstHHI GCGC 3 cut(s) 257, 340, 760
BstKTI GATC 2 cut(s) 9, 967
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 6, 964
BstMCI CGRYCG 1 cut(s) 789
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 2 cut(s) 86, 681
BstNSI RCATGY 2 cut(s) 93, 121
BstSCI CCNGG 2 cut(s) 84, 679
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 1 cut(s) 338
BstV1I GCAGC 2 cut(s) 491, 635
BstX2I RGATCY 2 cut(s) 6, 964
BstYI RGATCY 2 cut(s) 6, 964
BstZI CGGCCG 1 cut(s) 786
BsuI GTATCC 1 cut(s) 803
BsuRI GGCC 4 cut(s) 104, 276, 572, 788
BtsCI GGATG 3 cut(s) 267, 325, 925
BtsIMutI CAGTG 2 cut(s) 144, 188
Cac8I GCNNGC 3 cut(s) 30, 91, 102
CfoI GCGC 3 cut(s) 257, 340, 760
Cfr10I RCCGGY 1 cut(s) 765
CseI GACGC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 799
CspAI ACCGGT 1 cut(s) 765
CviAII CATG 5 cut(s) 90, 118, 133, 153, 814
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 6 cut(s) 71, 140, 545, 557, 718, 725
DpnI GATC 2 cut(s) 8, 966
DpnII GATC 2 cut(s) 6, 964
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 786
EagI CGGCCG 1 cut(s) 786
Eam1104I CTCTTC 3 cut(s) 682, 707, 821
EarI CTCTTC 3 cut(s) 682, 707, 821
EclXI CGGCCG 1 cut(s) 786
Eco130I CCWWGG 2 cut(s) 271, 959
Eco31I GGTCTC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 786
Eco88I CYCGRG 2 cut(s) 357, 493
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 2 cut(s) 84, 679
EcoT14I CCWWGG 2 cut(s) 271, 959
ErhI CCWWGG 2 cut(s) 271, 959
FaeI CATG 5 cut(s) 93, 121, 136, 156, 817
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FaqI GGGAC 2 cut(s) 482, 645
FatI CATG 5 cut(s) 89, 117, 132, 152, 813
Fnu4HI GCNGC 3 cut(s) 505, 624, 786
FokI GGATG 3 cut(s) 254, 332, 932
Fsp4HI GCNGC 3 cut(s) 505, 624, 786
FspBI CTAG 6 cut(s) 296, 524, 576, 597, 635, 960
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 256, 339, 759
GluI GCNGC 3 cut(s) 505, 624, 786
GsaI CCCAGC 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 761
HaeIII GGCC 4 cut(s) 104, 276, 572, 788
HapII CCGG 2 cut(s) 766, 789
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 257, 340, 760
Hin1II CATG 5 cut(s) 93, 121, 136, 156, 817
Hin6I GCGC 3 cut(s) 255, 338, 758
HinP1I GCGC 3 cut(s) 255, 338, 758
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 4 cut(s) 149, 451, 643, 850
HpaII CCGG 2 cut(s) 766, 789
HphI GGTGA 2 cut(s) 668, 755
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 3 cut(s) 74, 773, 855
Hpy188III TCNNGA 6 cut(s) 10, 211, 478, 495, 649, 750
Hpy8I GTNNAC 1 cut(s) 676
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 3 cut(s) 69, 832, 904
HpyCH4IV ACGT 3 cut(s) 20, 442, 738
HpyCH4V TGCA 5 cut(s) 290, 425, 507, 885, 945
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 6 cut(s) 71, 140, 545, 557, 718, 725
HpySE526I ACGT 3 cut(s) 20, 442, 738
Hsp92II CATG 5 cut(s) 93, 121, 136, 156, 817
HspAI GCGC 3 cut(s) 255, 338, 758
Kzo9I GATC 2 cut(s) 6, 964
LguI GCTCTTC 2 cut(s) 682, 707
LmnI GCTCC 1 cut(s) 869
Lsp1109I GCAGC 2 cut(s) 491, 635
LweI GCATC 1 cut(s) 333
MaeI CTAG 6 cut(s) 296, 524, 576, 597, 635, 960
MaeII ACGT 3 cut(s) 20, 442, 738
MaeIII GTNAC 5 cut(s) 63, 438, 455, 509, 953
MalI GATC 2 cut(s) 8, 966
MboI GATC 2 cut(s) 6, 964
MboII GAAGA 6 cut(s) 206, 293, 311, 699, 724, 838
MflI RGATCY 2 cut(s) 6, 964
MhlI GDGCHC 1 cut(s) 666
MluCI AATT 3 cut(s) 181, 197, 369
MseI TTAA 4 cut(s) 159, 582, 834, 879
MslI CAYNNNNRTG 2 cut(s) 669, 681
MspA1I CMGCKG 1 cut(s) 626
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 2 cut(s) 766, 789
MspR9I CCNGG 2 cut(s) 86, 681
MvaI CCWGG 2 cut(s) 86, 681
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 346
NdeII GATC 2 cut(s) 6, 964
NlaIII CATG 5 cut(s) 93, 121, 136, 156, 817
NlaIV GGNNCC 1 cut(s) 8
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 438
NsbI TGCGCA 1 cut(s) 256
NspI RCATGY 2 cut(s) 93, 121
OliI CACNNNNGTG 2 cut(s) 669, 681
PaeI GCATGC 1 cut(s) 93
PciSI GCTCTTC 2 cut(s) 682, 707
PfeI GAWTC 4 cut(s) 149, 451, 643, 850
PinAI ACCGGT 1 cut(s) 765
PkrI GCNGC 3 cut(s) 506, 625, 787
Ppu21I YACGTR 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 84, 679
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 2 cut(s) 84, 679
PspN4I GGNNCC 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 586, 618
PstI CTGCAG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 6, 964
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 2 cut(s) 669, 681
SapI GCTCTTC 2 cut(s) 682, 707
SaqAI TTAA 4 cut(s) 159, 582, 834, 879
SatI GCNGC 3 cut(s) 505, 624, 786
Sau3AI GATC 2 cut(s) 6, 964
ScrFI CCNGG 2 cut(s) 86, 681
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 669, 681
SmlI CTYRAG 2 cut(s) 647, 833
SmoI CTYRAG 2 cut(s) 647, 833
SpeI ACTAGT 1 cut(s) 596
SphI GCATGC 1 cut(s) 93
Sse9I AATT 3 cut(s) 181, 197, 369
SsiI CCGC 3 cut(s) 172, 785, 983
SspI AATATT 2 cut(s) 589, 892
SspMI CTAG 6 cut(s) 296, 524, 576, 597, 635, 960
StyD4I CCNGG 2 cut(s) 84, 679
StyI CCWWGG 2 cut(s) 271, 959
TaiI ACGT 3 cut(s) 23, 445, 741
TaqI TCGA 2 cut(s) 387, 410
TasI AATT 3 cut(s) 181, 197, 369
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 4 cut(s) 149, 451, 643, 850
Tru1I TTAA 4 cut(s) 159, 582, 834, 879
Tru9I TTAA 4 cut(s) 159, 582, 834, 879
TscAI CASTG 2 cut(s) 151, 195
TseFI GTSAC 1 cut(s) 438
TseI GCWGC 2 cut(s) 504, 623
Tsp45I GTSAC 1 cut(s) 438
TspDTI ATGAA 4 cut(s) 141, 149, 318, 923
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 2 cut(s) 151, 195
Vha464I CTTAAG 1 cut(s) 833
XapI RAATTY 2 cut(s) 181, 197
XceI RCATGY 2 cut(s) 93, 121
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 959
XspI CTAG 6 cut(s) 296, 524, 576, 597, 635, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.