pycom07g18750

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
21144261 .. 21145741
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGACTTCGTATGCTGATCAAAACAATGAAAGCCGAGATGAATGTCATCCTTCGCGTTCTAGTGACTACAATACTACCTCTTGGATTGGCTTCGGGGTTGTGATTCGCGTGAGTACAAATCCTTGCATGTTTCTTCTCAGTCCAATTCTTTGTCATTCCATGCCTCATCAAATGGAAGTGATTGAGTCGGCTGTGCAATTTAAAAGAGGCTCTGAGGAAGCAAGTAGGTTTCTTCCGAATGGTAATGGACAGATTGTTAATTTGGAAAGTAACACGATGTTACTTAACGAGGGGAAGGCAGATGATATACGTATGGGAGCTGAGGTAAAGAAGAAGAATGATTCGGAAATTGGTGATACGGTTTTCACAAATTGCGCACAAGAACAATCTGCTCTTCGTGAAACATTGCAGAACGGGACAATCAAGAATGTACAACAGCATGGTCAGTCGAATGAGGCCAGTGGTAGAAAAGGCCGTGGAAAGAAGTGGGGATGTAAAAAGGAAGTGGTGGATTTGAGAACTCTTTTGACTCTTTGTGCACGAGCGGTTGCAGCTAATGATAAGAGGATGCGCAATGAGTTGCTTAAGCATATCAGACAGCATTCTTCTCTTATGGCAATGGGAAACCCGCTGCAAAGACTAGCTCACTATTTTGCTGATGGTCTTGAGGCACGCATGGCTGGATCCGGTGCTCAGATTTACAAAGATCTTGTGTCCAAGCTAGCATCAGCTGCAGATGTCTTGAAGGCTTACTATCTATATCTTGTCGGCCTCTTCCACTTTTCCACATTGTTCGATATGAAGATAAATGTCCCGCATGATGCTCCAGAGAGGATTTTGATTGCGAGGGAGGTCTTCGGTAAGCAGGCAATGAATGTCATTGCATGTGAGGGTTCGGAGAGGATTGAGAGACCAGACATACAGGCAGTGGCAGGTGAGGAACCTGAGGGCCGGGTTTACGCAACTTTGAATCAGGAGATTGCGAAGGTAGCAAAGGACATCCTCTCTCTGTCATTTGTCAATTTGTGTCACGTAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

347

Amino Acids

38.49

Weight (kDa)

7.62

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 172 - 254 8.8e-14 GRAS domain family
GRAS PF03514 258 - 305 4.2e-07 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 923
Acc16I TGCGCA 2 cut(s) 376, 572
Acc36I ACCTGC 1 cut(s) 923
AccBSI CCGCTC 1 cut(s) 545
AccII CGCG 2 cut(s) 55, 108
AciI CCGC 3 cut(s) 545, 629, 815
AclWI GGATC 2 cut(s) 678, 691
AfaI GTAC 2 cut(s) 115, 432
AflII CTTAAG 1 cut(s) 584
AgsI TTSAA 2 cut(s) 745, 970
AluBI AGCT 5 cut(s) 320, 554, 644, 721, 731
AluI AGCT 5 cut(s) 320, 554, 644, 721, 731
Alw21I GWGCWC 2 cut(s) 541, 694
Alw26I GTCTC 1 cut(s) 904
Alw44I GTGCAC 1 cut(s) 537
AlwI GGATC 2 cut(s) 678, 691
AoxI GGCC 4 cut(s) 456, 472, 769, 949
ApaLI GTGCAC 1 cut(s) 537
ApeKI GCWGC 3 cut(s) 551, 631, 731
AspLEI GCGC 2 cut(s) 377, 573
AspS9I GGNCC 1 cut(s) 949
AsuC2I CCSGG 1 cut(s) 953
AsuHPI GGTGA 2 cut(s) 365, 947
AsuNHI GCTAGC 1 cut(s) 721
AxyI CCTNAGG 1 cut(s) 945
BaeGI GKGCMC 1 cut(s) 541
BamHI GGATCC 1 cut(s) 683
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 1 cut(s) 847
Bbv12I GWGCWC 2 cut(s) 541, 694
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 3 cut(s) 563, 618, 718
BccI CCATC 1 cut(s) 653
BceAI ACGGC 1 cut(s) 459
BclI TGATCA 1 cut(s) 16
BcnI CCSGG 1 cut(s) 953
BcoDI GTCTC 1 cut(s) 904
BfaI CTAG 3 cut(s) 60, 641, 722
BfmI CTRYAG 1 cut(s) 732
BfrI CTTAAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 923
BglII AGATCT 1 cut(s) 706
BisI GCNGC 3 cut(s) 552, 632, 732
BlsI GCNGC 3 cut(s) 553, 633, 733
Bme1390I CCNGG 1 cut(s) 953
BmgT120I GGNCC 1 cut(s) 949
BmiI GGNNCC 2 cut(s) 685, 942
BmrFI CCNGG 1 cut(s) 953
BmsI GCATC 3 cut(s) 558, 734, 811
BmtI GCTAGC 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 847
BpmI CTGGAG 1 cut(s) 810
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 953
BsaAI YACGTR 2 cut(s) 311, 1033
BsaI GGTCTC 1 cut(s) 904
BsaJI CCNNGG 1 cut(s) 475
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bse1I ACTGG 1 cut(s) 459
Bse21I CCTNAGG 1 cut(s) 945
Bse3DI GCAATG 5 cut(s) 404, 580, 624, 876, 879
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 4 cut(s) 46, 497, 573, 999
BseMI GCAATG 5 cut(s) 404, 580, 624, 876, 879
BseMII CTCAG 5 cut(s) 151, 204, 312, 707, 936
BseNI ACTGG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 3 cut(s) 563, 618, 718
Bsh1236I CGCG 2 cut(s) 55, 108
BshFI GGCC 4 cut(s) 458, 474, 771, 951
BsiHKAI GWGCWC 2 cut(s) 541, 694
BsiSI CCGG 2 cut(s) 687, 952
BslFI GGGAC 2 cut(s) 430, 797
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 2 cut(s) 430, 797
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 4 cut(s) 458, 474, 771, 951
Bso31I GGTCTC 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 541, 694
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 3 cut(s) 16, 683, 706
BspACI CCGC 3 cut(s) 545, 629, 815
BspANI GGCC 4 cut(s) 458, 474, 771, 951
BspCNI CTCAG 5 cut(s) 150, 205, 313, 706, 937
BspFNI CGCG 2 cut(s) 55, 108
BspLI GGNNCC 2 cut(s) 685, 942
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 1 cut(s) 923
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 2 cut(s) 678, 691
BspQI GCTCTTC 1 cut(s) 399
BspTI CTTAAG 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 904
BsrBI CCGCTC 1 cut(s) 545
BsrDI GCAATG 5 cut(s) 404, 580, 624, 876, 879
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 3 cut(s) 16, 683, 706
BssSI CACGAG 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 540
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 2 cut(s) 399, 779
BstAFI CTTAAG 1 cut(s) 584
BstAPI GCANNNNNTGC 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 430
BstBAI YACGTR 2 cut(s) 311, 1033
BstC8I GCNNGC 3 cut(s) 673, 723, 867
BstDEI CTNAG 5 cut(s) 137, 213, 321, 693, 945
BstDSI CCRYGG 1 cut(s) 475
BstF5I GGATG 4 cut(s) 46, 497, 573, 999
BstFNI CGCG 2 cut(s) 55, 108
BstHHI GCGC 2 cut(s) 377, 573
BstKTI GATC 3 cut(s) 19, 686, 709
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 3 cut(s) 16, 683, 706
BstMWI GCNNNNNNNGC 4 cut(s) 551, 677, 731, 989
BstNSI RCATGY 2 cut(s) 130, 888
BstSCI CCNGG 1 cut(s) 951
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 541
BstSNI TACGTA 1 cut(s) 311
BstUI CGCG 2 cut(s) 55, 108
BstV1I GCAGC 3 cut(s) 563, 618, 718
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 2 cut(s) 683, 706
BstYI RGATCY 2 cut(s) 683, 706
Bsu36I CCTNAGG 1 cut(s) 945
BsuRI GGCC 4 cut(s) 458, 474, 771, 951
BtgI CCRYGG 1 cut(s) 475
BtsCI GGATG 4 cut(s) 46, 497, 573, 999
BtsI GCAGTG 1 cut(s) 933
BtsIMutI CAGTG 2 cut(s) 466, 933
BveI ACCTGC 1 cut(s) 923
Cac8I GCNNGC 3 cut(s) 673, 723, 867
CfoI GCGC 2 cut(s) 377, 573
Cfr13I GGNCC 1 cut(s) 949
Csp6I GTAC 2 cut(s) 114, 431
CviAII CATG 6 cut(s) 127, 160, 440, 676, 818, 885
CviQI GTAC 2 cut(s) 114, 431
DdeI CTNAG 5 cut(s) 137, 213, 321, 693, 945
DpnI GATC 3 cut(s) 18, 685, 708
DpnII GATC 3 cut(s) 16, 683, 706
DraI TTTAAA 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 399, 779
EarI CTCTTC 2 cut(s) 399, 779
Eco105I TACGTA 1 cut(s) 311
Eco31I GGTCTC 1 cut(s) 904
Eco81I CCTNAGG 1 cut(s) 945
FaeI CATG 6 cut(s) 130, 163, 443, 679, 821, 888
FaqI GGGAC 2 cut(s) 430, 797
FatI CATG 6 cut(s) 126, 159, 439, 675, 817, 884
FauI CCCGC 2 cut(s) 636, 822
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 552, 632, 732
FokI GGATG 4 cut(s) 33, 504, 580, 986
Fsp4HI GCNGC 3 cut(s) 552, 632, 732
FspBI CTAG 3 cut(s) 60, 641, 722
FspI TGCGCA 2 cut(s) 376, 572
GlaI GCGC 2 cut(s) 376, 572
GluI GCNGC 3 cut(s) 552, 632, 732
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 4 cut(s) 458, 474, 771, 951
HapII CCGG 2 cut(s) 687, 952
HhaI GCGC 2 cut(s) 377, 573
Hin1II CATG 6 cut(s) 130, 163, 443, 679, 821, 888
Hin6I GCGC 2 cut(s) 375, 571
HinP1I GCGC 2 cut(s) 375, 571
HinfI GANTC 5 cut(s) 103, 185, 341, 529, 970
HpaII CCGG 2 cut(s) 687, 952
HphI GGTGA 2 cut(s) 365, 947
Hpy166II GTNNAC 2 cut(s) 539, 958
Hpy188I TCNGA 6 cut(s) 214, 237, 346, 596, 696, 898
Hpy188III TCNNGA 6 cut(s) 398, 424, 665, 742, 827, 974
Hpy8I GTNNAC 2 cut(s) 539, 958
HpyAV CCTTC 4 cut(s) 60, 289, 739, 979
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 2 cut(s) 310, 1032
HpyCH4V TGCA 8 cut(s) 126, 196, 409, 539, 551, 634, 734, 884
HpyF10VI GCNNNNNNNGC 4 cut(s) 551, 677, 731, 989
HpyF3I CTNAG 5 cut(s) 137, 213, 321, 693, 945
HpySE526I ACGT 2 cut(s) 310, 1032
Hsp92II CATG 6 cut(s) 130, 163, 443, 679, 821, 888
HspAI GCGC 2 cut(s) 375, 571
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 16, 683, 706
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 2 cut(s) 317, 829
Lsp1109I GCAGC 3 cut(s) 563, 618, 718
LweI GCATC 3 cut(s) 558, 734, 811
MaeI CTAG 3 cut(s) 60, 641, 722
MaeII ACGT 2 cut(s) 310, 1032
MaeIII GTNAC 4 cut(s) 62, 269, 279, 1028
MalI GATC 3 cut(s) 18, 685, 708
MbiI CCGCTC 1 cut(s) 545
MboI GATC 3 cut(s) 16, 683, 706
MboII GAAGA 9 cut(s) 125, 224, 343, 346, 386, 597, 766, 814, 847
MflI RGATCY 2 cut(s) 683, 706
MhlI GDGCHC 2 cut(s) 541, 694
MluCI AATT 6 cut(s) 144, 197, 259, 348, 370, 1021
MlyI GAGTC 2 cut(s) 194, 523
MseI TTAA 4 cut(s) 201, 258, 285, 585
MspA1I CMGCKG 2 cut(s) 631, 731
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 2 cut(s) 687, 952
MspR9I CCNGG 1 cut(s) 953
Mva1269I GAATGC 1 cut(s) 601
MvnI CGCG 2 cut(s) 55, 108
MwoI GCNNNNNNNGC 4 cut(s) 551, 677, 731, 989
NciI CCSGG 1 cut(s) 953
NdeII GATC 3 cut(s) 16, 683, 706
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 6 cut(s) 130, 163, 443, 679, 821, 888
NlaIV GGNNCC 2 cut(s) 685, 942
NmeAIII GCCGAG 1 cut(s) 59
NmuCI GTSAC 2 cut(s) 62, 1028
NsbI TGCGCA 2 cut(s) 376, 572
NspI RCATGY 2 cut(s) 130, 888
PaqCI CACCTGC 1 cut(s) 923
PciSI GCTCTTC 1 cut(s) 399
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 3 cut(s) 103, 341, 970
PkrI GCNGC 3 cut(s) 553, 633, 733
PleI GAGTC 2 cut(s) 193, 523
PpsI GAGTC 2 cut(s) 193, 523
Ppu21I YACGTR 2 cut(s) 311, 1033
PspN4I GGNNCC 2 cut(s) 685, 942
PspPI GGNCC 1 cut(s) 949
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 683, 706
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 2 cut(s) 115, 432
RsaNI GTAC 2 cut(s) 114, 431
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 4 cut(s) 201, 258, 285, 585
SatI GCNGC 3 cut(s) 552, 632, 732
Sau3AI GATC 3 cut(s) 16, 683, 706
Sau96I GGNCC 1 cut(s) 949
SchI GAGTC 2 cut(s) 194, 523
ScrFI CCNGG 1 cut(s) 953
SduI GDGCHC 2 cut(s) 541, 694
SfaNI GCATC 3 cut(s) 558, 734, 811
SfcI CTRYAG 1 cut(s) 732
SmlI CTYRAG 2 cut(s) 584, 665
SmoI CTYRAG 2 cut(s) 584, 665
SnaBI TACGTA 1 cut(s) 311
Sse9I AATT 6 cut(s) 144, 197, 259, 348, 370, 1021
SsiI CCGC 3 cut(s) 545, 629, 815
SspMI CTAG 3 cut(s) 60, 641, 722
StyD4I CCNGG 1 cut(s) 951
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 2 cut(s) 313, 1035
TaqI TCGA 2 cut(s) 449, 795
TasI AATT 6 cut(s) 144, 197, 259, 348, 370, 1021
TatI WGTACW 2 cut(s) 113, 430
TfiI GAWTC 3 cut(s) 103, 341, 970
Tru1I TTAA 4 cut(s) 201, 258, 285, 585
Tru9I TTAA 4 cut(s) 201, 258, 285, 585
TscAI CASTG 2 cut(s) 466, 933
TseFI GTSAC 2 cut(s) 62, 1028
TseI GCWGC 3 cut(s) 551, 631, 731
Tsp45I GTSAC 2 cut(s) 62, 1028
TspDTI ATGAA 4 cut(s) 42, 54, 815, 887
TspRI CASTG 2 cut(s) 466, 933
Vha464I CTTAAG 1 cut(s) 584
VneI GTGCAC 1 cut(s) 537
XceI RCATGY 2 cut(s) 130, 888
XspI CTAG 3 cut(s) 60, 641, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.