pycom07g20980

membrane protein At1g06890-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
22890970 .. 22893118
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGGGGGAAGGCGAGAGGTTTCAGCTGGGGACAGTGGGAGCGTTAACCCTCTCTGTGGTCTCATCGGTCTCCATTGTCATTTGCAACAAGGCCCTCATCAGTTCTTTGGGTTTCACTTTTGCCACAACTTTGACAAGCTGGCATCTTTTAGTCACCTTTTGTTCCCTTCACGTGGCTTTAAAGATGAAATTCTTTGAACACAAACCGATCGATCAAACGACTGTTACGGGGTTTGGCATTCTGAATGGAATCTCAATTGGACTGTTAAATCTGAGCCTGGGCTTCAATTCGGTTGGCTTTTATCAGATGACTAAGCTGGCAATCATCCCTTGCACTGTACTATTGGAGACCCTTTTCCTCGCGAAGAAATTCAGTAGGAGCATTCAGGTTGCCCTCCTTGTTCTCCTCATTGGTGTTGGGATTGCAACAGTCACTGATGTTCAACTTAATGCTCTTGGAAGTATCTTGTCTCTTCTTGCAGTTATTACAACATGCGTTGCTCAAATTATGACCAATACCATCCAGAAGAAGTTTAAGGTTTCTTCAACTCAACTCCTGTATCAATCATGTCCATATCAGTCAGGAACTTTATTAATCACTGGCCCGTTTCTGGATTGGTTCTTGACGAATCAAAATGTATTTGCTTTCAAGTATACAAATCAAGTGTTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPT

Protein Analysis

225

Amino Acids

24.33

Weight (kDa)

9.18

Isoelectric Point (pI)

17.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 21 - 181 2.8e-09 Triose-phosphate Transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 653
AccII CGCG 1 cut(s) 362
AcsI RAATTY 2 cut(s) 188, 368
AcvI CACGTG 1 cut(s) 172
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 3 cut(s) 55, 172, 610
AgsI TTSAA 5 cut(s) 197, 286, 443, 546, 649
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 3 cut(s) 25, 138, 316
AluI AGCT 3 cut(s) 25, 138, 316
Alw26I GTCTC 4 cut(s) 64, 73, 341, 474
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 2 cut(s) 90, 601
ApoI RAATTY 2 cut(s) 188, 368
AseI ATTAAT 1 cut(s) 593
Asp700I GAANNNNTTC 1 cut(s) 368
AspS9I GGNCC 2 cut(s) 91, 602
AsuHPI GGTGA 1 cut(s) 145
BaeI ACNNNNGTAYC 2 cut(s) 542, 575
BbrPI CACGTG 1 cut(s) 172
BccI CCATC 1 cut(s) 527
BciT130I CCWGG 1 cut(s) 278
BcoDI GTCTC 4 cut(s) 64, 73, 341, 474
Bme1390I CCNGG 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 91, 602
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 151
Bsa29I ATCGAT 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 172
BsaI GGTCTC 3 cut(s) 64, 73, 341
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 172, 610
Bse1I ACTGG 1 cut(s) 604
BseBI CCWGG 1 cut(s) 278
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 324, 519
BseLI CCNNNNNNNGG 3 cut(s) 55, 172, 610
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 1 cut(s) 395
BseYI CCCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 2 cut(s) 92, 603
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 3 cut(s) 55, 172, 610
BsmAI GTCTC 4 cut(s) 64, 73, 341, 474
BsmFI GGGAC 1 cut(s) 43
BsmI GAATGC 2 cut(s) 237, 381
BsnI GGCC 2 cut(s) 92, 603
Bso31I GGTCTC 3 cut(s) 64, 73, 341
Bsp143I GATC 2 cut(s) 207, 211
Bsp68I TCGCGA 1 cut(s) 362
BspANI GGCC 2 cut(s) 92, 603
BspCNI CTCAG 1 cut(s) 264
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 362
BspTNI GGTCTC 3 cut(s) 64, 73, 341
BsrI ACTGG 1 cut(s) 604
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 2 cut(s) 207, 211
BssNAI GTATAC 1 cut(s) 654
Bst1107I GTATAC 1 cut(s) 654
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 5 cut(s) 34, 223, 264, 337, 430
Bst6I CTCTTC 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 172
BstC8I GCNNGC 2 cut(s) 140, 318
BstDEI CTNAG 2 cut(s) 272, 312
BstF5I GGATG 2 cut(s) 324, 519
BstFNI CGCG 1 cut(s) 362
BstKTI GATC 2 cut(s) 210, 214
BstMAI GTCTC 4 cut(s) 64, 73, 341, 474
BstMBI GATC 2 cut(s) 207, 211
BstMCI CGRYCG 1 cut(s) 210
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 495
BstSCI CCNGG 1 cut(s) 276
BstUI CGCG 1 cut(s) 362
BstZ17I GTATAC 1 cut(s) 654
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 2 cut(s) 92, 603
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 2 cut(s) 324, 519
BtsIMutI CAGTG 4 cut(s) 39, 333, 432, 597
BtuMI TCGCGA 1 cut(s) 362
Cac8I GCNNGC 2 cut(s) 140, 318
CaiI CAGNNNCTG 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 91, 602
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 1 cut(s) 338
CviAII CATG 2 cut(s) 492, 567
CviJI RGCY 9 cut(s) 25, 92, 138, 176, 276, 282, 297, 316, 603
CviKI_1 RGCY 9 cut(s) 25, 92, 138, 176, 276, 282, 297, 316, 603
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 2 cut(s) 272, 312
DpnI GATC 2 cut(s) 209, 213
DpnII GATC 2 cut(s) 207, 211
DraI TTTAAA 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco31I GGTCTC 3 cut(s) 64, 73, 341
Eco72I CACGTG 1 cut(s) 172
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 276
FaeI CATG 2 cut(s) 495, 570
FaiI YATR 6 cut(s) 493, 509, 568, 574, 654, 673
FaqI GGGAC 1 cut(s) 43
FatI CATG 2 cut(s) 491, 566
FblI GTMKAC 1 cut(s) 653
FokI GGATG 2 cut(s) 311, 506
GsaI CCCAGC 1 cut(s) 29
HaeIII GGCC 2 cut(s) 92, 603
Hin1II CATG 2 cut(s) 495, 570
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 2 cut(s) 249, 628
HpaI GTTAAC 1 cut(s) 45
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 2 cut(s) 45, 654
Hpy188I TCNGA 3 cut(s) 243, 273, 306
Hpy188III TCNNGA 5 cut(s) 361, 523, 582, 611, 622
Hpy8I GTNNAC 2 cut(s) 45, 654
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 5 cut(s) 34, 223, 264, 337, 430
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 4 cut(s) 84, 333, 425, 479
HpyF3I CTNAG 2 cut(s) 272, 312
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 495, 570
KspAI GTTAAC 1 cut(s) 45
Kzo9I GATC 2 cut(s) 207, 211
LmnI GCTCC 2 cut(s) 38, 378
LweI GCATC 1 cut(s) 151
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 3 cut(s) 151, 223, 430
MalI GATC 2 cut(s) 209, 213
MboI GATC 2 cut(s) 207, 211
MboII GAAGA 4 cut(s) 376, 464, 534, 538
MfeI CAATTG 1 cut(s) 255
MluCI AATT 5 cut(s) 188, 255, 286, 368, 504
MnlI CCTC 6 cut(s) 9, 59, 104, 368, 404, 416
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 6 cut(s) 44, 179, 266, 447, 534, 593
MspA1I CMGCKG 1 cut(s) 25
MspR9I CCNGG 1 cut(s) 278
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 2 cut(s) 237, 381
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 362
NdeII GATC 2 cut(s) 207, 211
NlaIII CATG 2 cut(s) 495, 570
NmuCI GTSAC 2 cut(s) 151, 430
NruI TCGCGA 1 cut(s) 362
NspI RCATGY 1 cut(s) 495
PctI GAATGC 2 cut(s) 237, 381
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 249, 628
Ple19I CGATCG 1 cut(s) 210
PmaCI CACGTG 1 cut(s) 172
PmlI CACGTG 1 cut(s) 172
Ppu21I YACGTR 1 cut(s) 172
PshBI ATTAAT 1 cut(s) 593
Psp6I CCWGG 1 cut(s) 276
PspCI CACGTG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 25
PspGI CCWGG 1 cut(s) 276
PspPI GGNCC 2 cut(s) 91, 602
PstNI CAGNNNCTG 1 cut(s) 434
PvuI CGATCG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 25
RruI TCGCGA 1 cut(s) 362
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SaqAI TTAA 6 cut(s) 44, 179, 266, 447, 534, 593
Sau3AI GATC 2 cut(s) 207, 211
Sau96I GGNCC 2 cut(s) 91, 602
ScrFI CCNGG 1 cut(s) 278
SetI ASST 8 cut(s) 20, 27, 140, 158, 174, 318, 390, 540
SfaNI GCATC 1 cut(s) 151
Sse9I AATT 5 cut(s) 188, 255, 286, 368, 504
StyD4I CCNGG 1 cut(s) 276
TaaI ACNGT 5 cut(s) 34, 223, 264, 337, 430
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 210
TaqII GACCGA 1 cut(s) 55
TasI AATT 5 cut(s) 188, 255, 286, 368, 504
TatI WGTACW 1 cut(s) 337
TfiI GAWTC 2 cut(s) 249, 628
Tru1I TTAA 6 cut(s) 44, 179, 266, 447, 534, 593
Tru9I TTAA 6 cut(s) 44, 179, 266, 447, 534, 593
TscAI CASTG 4 cut(s) 39, 340, 439, 604
TseFI GTSAC 2 cut(s) 151, 430
Tsp45I GTSAC 2 cut(s) 151, 430
TspDTI ATGAA 2 cut(s) 200, 660
TspRI CASTG 4 cut(s) 39, 340, 439, 604
VspI ATTAAT 1 cut(s) 593
XapI RAATTY 2 cut(s) 188, 368
XceI RCATGY 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.