pycom07g21710

positive regulation of polynucleotide adenylyltransferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
23320081 .. 23320854
774 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGTCCAATTTGCGGGTAGCGGTTCTTAAAACGTGCCAATCGTCCCAACGACGCCGTTTAGAACACCTGCAGCAAAACGAAATAGAAGAAGCGAACTGCAGCCCCCCAAACCCCCAAACACACCGACCGCCCGATCCCAAATATATAATATCCCCAAAACCAAAAACCTCAAAAACCAGACGCACGCGCGCTCTCCGAATCACACTGAATCCGACAAACACCGGGGGAGAGAGAGCTATGGAGGAGCAACATGAGCACGAGGTTTACGGCGCCGAGATCCCCAACGACGACGGCGAAGACGTCGACATGTCGTCCAGGGCCGACGGGGCCGAAGACGAAGACTACAACGACGACCCTAACTCCAAGGAGCTGGAGGACATGAAGAAGCGGCTGAAAGAGATAGAGGAGGAAGCCGGCGCTCTCCGCGACATGCAGGCCAAGGTCGAGAAGGAGATGGGCGCTGTACAAGGTATCCAATTTTACTTTTCGTTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko05164 map03015 map05164
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.87

Weight (kDa)

5.04

Isoelectric Point (pI)

63.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 75
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 303
AccII CGCG 3 cut(s) 187, 189, 426
AciI CCGC 5 cut(s) 13, 20, 128, 388, 424
AclWI GGATC 2 cut(s) 128, 271
AcyI GRCGYC 3 cut(s) 52, 270, 300
AfaI GTAC 1 cut(s) 465
AfiI CCNNNNNNNGG 1 cut(s) 12
AflIII ACRYGT 1 cut(s) 306
AhdI GACNNNNNGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 314
AluBI AGCT 2 cut(s) 236, 370
AluI AGCT 2 cut(s) 236, 370
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 2 cut(s) 128, 271
AoxI GGCC 3 cut(s) 318, 327, 435
ApeKI GCWGC 2 cut(s) 70, 99
AspLEI GCGC 5 cut(s) 189, 191, 272, 419, 461
AspS9I GGNCC 2 cut(s) 318, 327
AsuC2I CCSGG 1 cut(s) 223
BanI GGYRCC 1 cut(s) 269
BauI CACGAG 1 cut(s) 257
BbsI GAAGAC 3 cut(s) 303, 339, 345
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 2 cut(s) 82, 111
BccI CCATC 1 cut(s) 448
BceAI ACGGC 3 cut(s) 39, 283, 307
BciT130I CCWGG 1 cut(s) 316
BciVI GTATCC 1 cut(s) 482
BcnI CCSGG 1 cut(s) 223
BfmI CTRYAG 2 cut(s) 68, 97
BfoI RGCGCY 3 cut(s) 273, 420, 462
BfuAI ACCTGC 1 cut(s) 75
BfuI GTATCC 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 3 cut(s) 71, 100, 389
BlsI GCNGC 3 cut(s) 72, 101, 390
Bme1390I CCNGG 2 cut(s) 223, 316
BmeRI GACNNNNNGTC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 318, 327
BmiI GGNNCC 2 cut(s) 271, 328
BmrFI CCNGG 2 cut(s) 223, 316
BpiI GAAGAC 3 cut(s) 303, 339, 345
BpmI CTGGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 3 cut(s) 52, 270, 300
BsaJI CCNNGG 4 cut(s) 222, 315, 363, 438
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse118I RCCGGY 1 cut(s) 413
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 4 cut(s) 222, 315, 363, 438
BseLI CCNNNNNNNGG 1 cut(s) 12
BsePI GCGCGC 1 cut(s) 187
BseRI GAGGAG 2 cut(s) 257, 419
BseXI GCAGC 2 cut(s) 82, 111
Bsh1236I CGCG 3 cut(s) 187, 189, 426
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 3 cut(s) 320, 329, 437
BshNI GGYRCC 1 cut(s) 269
BsiEI CGRYCG 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 2 cut(s) 222, 414
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 28
BsnI GGCC 3 cut(s) 320, 329, 437
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 463
Bsp143I GATC 2 cut(s) 133, 276
BspACI CCGC 5 cut(s) 13, 20, 128, 388, 424
BspANI GGCC 3 cut(s) 320, 329, 437
BspFNI CGCG 3 cut(s) 187, 189, 426
BspLI GGNNCC 2 cut(s) 271, 328
BspMAI CTGCAG 2 cut(s) 72, 101
BspMI ACCTGC 1 cut(s) 75
BspPI GGATC 2 cut(s) 128, 271
BspT107I GGYRCC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 413
BsrGI TGTACA 1 cut(s) 463
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 4 cut(s) 222, 315, 363, 438
BssHII GCGCGC 1 cut(s) 187
BssMI GATC 2 cut(s) 133, 276
BssNI GRCGYC 3 cut(s) 52, 270, 300
BssSI CACGAG 1 cut(s) 257
BssT1I CCWWGG 2 cut(s) 363, 438
Bst2BI CACGAG 1 cut(s) 257
Bst2UI CCWGG 1 cut(s) 316
BstACI GRCGYC 3 cut(s) 52, 270, 300
BstAUI TGTACA 1 cut(s) 463
BstC8I GCNNGC 4 cut(s) 185, 189, 415, 435
BstFNI CGCG 3 cut(s) 187, 189, 426
BstH2I RGCGCY 3 cut(s) 273, 420, 462
BstHHI GCGC 5 cut(s) 189, 191, 272, 419, 461
BstKTI GATC 2 cut(s) 136, 279
BstMBI GATC 2 cut(s) 133, 276
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 3 cut(s) 253, 326, 423
BstNI CCWGG 1 cut(s) 316
BstNSI RCATGY 2 cut(s) 310, 433
BstSCI CCNGG 2 cut(s) 221, 314
BstSFI CTRYAG 2 cut(s) 68, 97
BstUI CGCG 3 cut(s) 187, 189, 426
BstV1I GCAGC 2 cut(s) 82, 111
BstV2I GAAGAC 3 cut(s) 303, 339, 345
BstX2I RGATCY 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 370
BstYI RGATCY 1 cut(s) 276
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 3 cut(s) 320, 329, 437
BtsIMutI CAGTG 1 cut(s) 203
BveI ACCTGC 1 cut(s) 75
Cac8I GCNNGC 4 cut(s) 185, 189, 415, 435
CfoI GCGC 5 cut(s) 189, 191, 272, 419, 461
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 2 cut(s) 318, 327
CseI GACGC 2 cut(s) 60, 189
Csp6I GTAC 1 cut(s) 464
CviAII CATG 4 cut(s) 251, 307, 379, 430
CviJI RGCY 8 cut(s) 102, 236, 320, 329, 370, 391, 413, 437
CviKI_1 RGCY 8 cut(s) 102, 236, 320, 329, 370, 391, 413, 437
CviQI GTAC 1 cut(s) 464
DinI GGCGCC 1 cut(s) 271
DpnI GATC 2 cut(s) 135, 278
DpnII GATC 2 cut(s) 133, 276
DriI GACNNNNNGTC 1 cut(s) 310
Eam1105I GACNNNNNGTC 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 363, 438
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 2 cut(s) 363, 438
EgeI GGCGCC 1 cut(s) 271
EheI GGCGCC 1 cut(s) 271
ErhI CCWWGG 2 cut(s) 363, 438
FaeI CATG 4 cut(s) 254, 310, 382, 433
FaiI YATR 7 cut(s) 144, 146, 239, 252, 308, 380, 431
FaqI GGGAC 1 cut(s) 28
FatI CATG 4 cut(s) 250, 306, 378, 429
FauI CCCGC 1 cut(s) 6
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 3 cut(s) 71, 100, 389
Fsp4HI GCNGC 3 cut(s) 71, 100, 389
GlaI GCGC 5 cut(s) 188, 190, 271, 418, 460
GluI GCNGC 3 cut(s) 71, 100, 389
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 3 cut(s) 273, 420, 462
HaeIII GGCC 3 cut(s) 320, 329, 437
HapII CCGG 2 cut(s) 222, 414
HgaI GACGC 2 cut(s) 60, 189
HhaI GCGC 5 cut(s) 189, 191, 272, 419, 461
Hin1I GRCGYC 3 cut(s) 52, 270, 300
Hin1II CATG 4 cut(s) 254, 310, 382, 433
Hin6I GCGC 5 cut(s) 187, 189, 270, 417, 459
HinP1I GCGC 5 cut(s) 187, 189, 270, 417, 459
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 2 cut(s) 198, 208
HpaII CCGG 2 cut(s) 222, 414
Hpy166II GTNNAC 2 cut(s) 265, 304
Hpy188I TCNGA 2 cut(s) 197, 213
Hpy188III TCNNGA 1 cut(s) 445
Hpy8I GTNNAC 2 cut(s) 265, 304
Hpy99I CGWCG 6 cut(s) 54, 290, 293, 305, 326, 353
HpyAV CCTTC 1 cut(s) 442
HpyCH4IV ACGT 2 cut(s) 32, 300
HpyCH4V TGCA 3 cut(s) 70, 99, 433
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 326, 423
HpySE526I ACGT 2 cut(s) 32, 300
Hsp92I GRCGYC 3 cut(s) 52, 270, 300
Hsp92II CATG 4 cut(s) 254, 310, 382, 433
HspAI GCGC 5 cut(s) 187, 189, 270, 417, 459
KasI GGCGCC 1 cut(s) 269
KroI GCCGGC 1 cut(s) 413
KroNI GCCGGC 1 cut(s) 415
Kzo9I GATC 2 cut(s) 133, 276
LmnI GCTCC 2 cut(s) 244, 367
LpnPI CCDG 8 cut(s) 80, 190, 235, 301, 328, 356, 419, 427
Lsp1109I GCAGC 2 cut(s) 82, 111
MaeII ACGT 2 cut(s) 32, 300
MalI GATC 2 cut(s) 135, 278
MboI GATC 2 cut(s) 133, 276
MboII GAAGA 5 cut(s) 98, 308, 344, 350, 394
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 2 cut(s) 7, 476
Mly113I GGCGCC 1 cut(s) 270
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 6 cut(s) 178, 235, 253, 367, 397, 400
MroNI GCCGGC 1 cut(s) 413
MseI TTAA 1 cut(s) 27
MspI CCGG 2 cut(s) 222, 414
MspR9I CCNGG 2 cut(s) 223, 316
MvaI CCWGG 1 cut(s) 316
MvnI CGCG 3 cut(s) 187, 189, 426
MwoI GCNNNNNNNGC 3 cut(s) 253, 326, 423
NaeI GCCGGC 1 cut(s) 415
NarI GGCGCC 1 cut(s) 270
NciI CCSGG 1 cut(s) 223
NdeII GATC 2 cut(s) 133, 276
NgoMIV GCCGGC 1 cut(s) 413
NlaIII CATG 4 cut(s) 254, 310, 382, 433
NlaIV GGNNCC 2 cut(s) 271, 328
NmeAIII GCCGAG 1 cut(s) 298
NspI RCATGY 2 cut(s) 310, 433
PaqCI CACCTGC 1 cut(s) 75
PauI GCGCGC 1 cut(s) 187
PciI ACATGT 1 cut(s) 306
PcsI WCGNNNNNNNCGW 3 cut(s) 38, 291, 297
PdiI GCCGGC 1 cut(s) 415
PfeI GAWTC 2 cut(s) 198, 208
PkrI GCNGC 3 cut(s) 72, 101, 390
PluTI GGCGCC 1 cut(s) 273
PscI ACATGT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 271, 328
PspPI GGNCC 2 cut(s) 318, 327
PstI CTGCAG 2 cut(s) 72, 101
PsuI RGATCY 1 cut(s) 276
PteI GCGCGC 1 cut(s) 187
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 3 cut(s) 71, 100, 389
Sau3AI GATC 2 cut(s) 133, 276
Sau96I GGNCC 2 cut(s) 318, 327
ScrFI CCNGG 2 cut(s) 223, 316
SduI GDGCHC 1 cut(s) 258
SetI ASST 9 cut(s) 35, 69, 170, 238, 264, 303, 372, 444, 472
SfcI CTRYAG 2 cut(s) 68, 97
SfiI GGCCNNNNNGGCC 1 cut(s) 326
SfoI GGCGCC 1 cut(s) 271
Sse9I AATT 2 cut(s) 7, 476
SsiI CCGC 5 cut(s) 13, 20, 128, 388, 424
SspDI GGCGCC 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 221, 314
StyI CCWWGG 2 cut(s) 363, 438
TaiI ACGT 2 cut(s) 35, 303
TaqI TCGA 2 cut(s) 303, 444
TasI AATT 2 cut(s) 7, 476
TatI WGTACW 1 cut(s) 463
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 2 cut(s) 198, 208
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TscAI CASTG 1 cut(s) 210
TseI GCWGC 2 cut(s) 70, 99
TspDTI ATGAA 1 cut(s) 395
TspRI CASTG 1 cut(s) 210
XceI RCATGY 2 cut(s) 310, 433
XmiI GTMKAC 1 cut(s) 303
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.