pycom07g24340

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
25081249 .. 25081917
669 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
ATGCACTTCCACCACACACCATACATCCATGGCTTCCAACGGCGACCGCCTTCCGTACCACCCAACCTCCAAGCCCTCCAAACCCTCACTATTCCCTTGGATAAAACGCAACGTCTACACCTTGCCCGTCATCCTCTTTCTATGCATCTGTTTTTACTTCTTCGGCCTCTGGCAACACGACGACACCACCGCAGTCACCAAAACCGTCACCTCGCCAACCTTCAATCTCCCATGCAAATCCTCCAACGAAATCCAAACAACACAACAACAATCACGTTACCCCTAGATTTCAACTCCCACCATAGCCCCAACTTCACCGCCACCACGTCCTCCCACTCCGCCGCCACCAACGAGTACCCGTGCTGCAACGCGAAATACAGCGAGTACACGCCGTGCGAGGACTCCCAGAGGTCGCTGAAGTTCGACCGGGACAGGCTTATATACAGAGAGAGGCACTGCCCCGAGAAGAGCGAGTTCCTTAAGTGCCGCTTCCCGGCACCGTGCGGCTACAAAAACGTGTTCTCCTGGCCGAGAAGCCGGGATCTGGCGTGGTACGCCAATGTGCCGCACAAGGAGTTGACGGTGGAGAAGACACGTTCAAATTCCTTGGGGGAGGCACCATGTTCCCTCACGGTGCGGACGCGTACATTGATGATATTGGCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

223

Amino Acids

25.85

Weight (kDa)

9.9

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 128 - 202 3.2e-23 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 496, 616
AccI GTMKAC 1 cut(s) 115
AccII CGCG 2 cut(s) 371, 643
AciI CCGC 9 cut(s) 47, 190, 318, 339, 342, 487, 504, 566, 637
AclWI GGATC 1 cut(s) 549
AcoI YGGCCR 1 cut(s) 527
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 437
AdeI CACNNNGTG 1 cut(s) 393
AfaI GTAC 5 cut(s) 57, 356, 386, 554, 646
AfiI CCNNNNNNNGG 2 cut(s) 493, 544
AflII CTTAAG 1 cut(s) 479
AflIII ACRYGT 3 cut(s) 516, 593, 641
AgsI TTSAA 3 cut(s) 224, 292, 600
AjiI CACGTC 1 cut(s) 327
AjnI CCWGG 1 cut(s) 524
AlwI GGATC 1 cut(s) 549
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 164, 527
ApeKI GCWGC 1 cut(s) 363
ApoI RAATTY 1 cut(s) 601
AsuC2I CCSGG 3 cut(s) 428, 494, 539
AsuHPI GGTGA 3 cut(s) 188, 200, 307
AvaI CYCGRG 1 cut(s) 461
BanI GGYRCC 2 cut(s) 496, 616
BbsI GAAGAC 1 cut(s) 596
BbvI GCAGC 1 cut(s) 350
BceAI ACGGC 2 cut(s) 56, 376
BciT130I CCWGG 1 cut(s) 526
BcnI CCSGG 3 cut(s) 428, 494, 539
BfaI CTAG 1 cut(s) 284
BfrI CTTAAG 1 cut(s) 479
BisI GCNGC 5 cut(s) 342, 364, 487, 505, 566
BlsI GCNGC 5 cut(s) 343, 365, 488, 506, 567
Bme1390I CCNGG 4 cut(s) 428, 494, 526, 539
BmeT110I CYCGRG 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 327
BmiI GGNNCC 2 cut(s) 498, 618
BmrFI CCNGG 4 cut(s) 428, 494, 526, 539
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 596
BpuMI CCSGG 3 cut(s) 428, 494, 539
BsaJI CCNNGG 3 cut(s) 28, 96, 606
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 506, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 493, 544
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 28, 96, 606
BseGI GGATG 2 cut(s) 24, 130
BseLI CCNNNNNNNGG 2 cut(s) 493, 544
BseXI GCAGC 1 cut(s) 350
Bsh1236I CGCG 2 cut(s) 371, 643
Bsh1285I CGRYCG 2 cut(s) 47, 427
BshFI GGCC 2 cut(s) 166, 529
BshNI GGYRCC 2 cut(s) 496, 616
BsiEI CGRYCG 2 cut(s) 47, 427
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 427, 494, 538
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 2 cut(s) 493, 544
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 2 cut(s) 166, 529
BsoBI CYCGRG 1 cut(s) 461
Bsp143I GATC 1 cut(s) 541
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 9 cut(s) 47, 190, 318, 339, 342, 487, 504, 566, 637
BspANI GGCC 2 cut(s) 166, 529
BspFNI CGCG 2 cut(s) 371, 643
BspLI GGNNCC 2 cut(s) 498, 618
BspPI GGATC 1 cut(s) 549
BspQI GCTCTTC 1 cut(s) 461
BspT107I GGYRCC 2 cut(s) 496, 616
BspTI CTTAAG 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 28, 96, 606
BssMI GATC 1 cut(s) 541
BssT1I CCWWGG 3 cut(s) 28, 96, 606
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 4 cut(s) 206, 501, 583, 634
Bst6I CTCTTC 1 cut(s) 461
BstAFI CTTAAG 1 cut(s) 479
BstDSI CCRYGG 1 cut(s) 28
BstF5I GGATG 2 cut(s) 24, 130
BstFNI CGCG 2 cut(s) 371, 643
BstKTI GATC 1 cut(s) 544
BstMBI GATC 1 cut(s) 541
BstMCI CGRYCG 2 cut(s) 47, 427
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 1 cut(s) 526
BstSCI CCNGG 4 cut(s) 426, 492, 524, 537
BstUI CGCG 2 cut(s) 371, 643
BstV1I GCAGC 1 cut(s) 350
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuRI GGCC 2 cut(s) 166, 529
BtgI CCRYGG 1 cut(s) 28
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 2 cut(s) 24, 130
BtsI GCAGTG 1 cut(s) 454
BtsIMutI CAGTG 1 cut(s) 454
CseI GACGC 1 cut(s) 649
Csp6I GTAC 5 cut(s) 56, 355, 385, 553, 645
CviAII CATG 3 cut(s) 29, 232, 621
CviJI RGCY 8 cut(s) 33, 74, 166, 306, 436, 507, 529, 537
CviKI_1 RGCY 8 cut(s) 33, 74, 166, 306, 436, 507, 529, 537
CviQI GTAC 5 cut(s) 56, 355, 385, 553, 645
DpnI GATC 1 cut(s) 543
DpnII GATC 1 cut(s) 541
DraIII CACNNNGTG 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
EciI GGCGGA 1 cut(s) 328
Eco130I CCWWGG 3 cut(s) 28, 96, 606
Eco57I CTGAAG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 461
EcoRII CCWGG 1 cut(s) 524
EcoT14I CCWWGG 3 cut(s) 28, 96, 606
EcoT22I ATGCAT 1 cut(s) 147
ErhI CCWWGG 3 cut(s) 28, 96, 606
FaeI CATG 3 cut(s) 32, 235, 624
FaiI YATR 8 cut(s) 22, 30, 143, 233, 303, 440, 442, 622
FalI AAGNNNNNCTT 2 cut(s) 473, 505
FaqI GGGAC 1 cut(s) 443
FatI CATG 3 cut(s) 28, 231, 620
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 5 cut(s) 342, 364, 487, 505, 566
FokI GGATG 2 cut(s) 11, 117
Fsp4HI GCNGC 5 cut(s) 342, 364, 487, 505, 566
FspBI CTAG 1 cut(s) 284
GluI GCNGC 5 cut(s) 342, 364, 487, 505, 566
HaeIII GGCC 2 cut(s) 166, 529
HapII CCGG 3 cut(s) 427, 494, 538
HgaI GACGC 1 cut(s) 649
Hin1II CATG 3 cut(s) 32, 235, 624
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HinfI GANTC 1 cut(s) 401
HpaII CCGG 3 cut(s) 427, 494, 538
HphI GGTGA 3 cut(s) 188, 200, 307
Hpy166II GTNNAC 3 cut(s) 116, 387, 579
Hpy8I GTNNAC 3 cut(s) 116, 387, 579
Hpy99I CGWCG 1 cut(s) 183
HpyAV CCTTC 2 cut(s) 60, 230
HpyCH4III ACNGT 4 cut(s) 206, 501, 583, 634
HpyCH4IV ACGT 5 cut(s) 112, 275, 326, 516, 595
HpyCH4V TGCA 4 cut(s) 4, 145, 235, 366
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpySE526I ACGT 5 cut(s) 112, 275, 326, 516, 595
Hsp92II CATG 3 cut(s) 32, 235, 624
Kzo9I GATC 1 cut(s) 541
LguI GCTCTTC 1 cut(s) 461
LpnPI CCDG 9 cut(s) 155, 418, 419, 440, 507, 511, 530, 538, 551
Lsp1109I GCAGC 1 cut(s) 350
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 284
MaeII ACGT 5 cut(s) 112, 275, 326, 516, 595
MaeIII GTNAC 3 cut(s) 194, 206, 276
MalI GATC 1 cut(s) 543
MboI GATC 1 cut(s) 541
MboII GAAGA 3 cut(s) 152, 478, 601
MflI RGATCY 1 cut(s) 541
MluCI AATT 2 cut(s) 601, 664
MluI ACGCGT 1 cut(s) 641
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 61, 268
Mph1103I ATGCAT 1 cut(s) 147
MseI TTAA 1 cut(s) 480
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 3 cut(s) 427, 494, 538
MspR9I CCNGG 4 cut(s) 428, 494, 526, 539
MvaI CCWGG 1 cut(s) 526
MvnI CGCG 2 cut(s) 371, 643
MwoI GCNNNNNNNGC 1 cut(s) 554
NciI CCSGG 3 cut(s) 428, 494, 539
NcoI CCATGG 1 cut(s) 28
NdeII GATC 1 cut(s) 541
NlaIII CATG 3 cut(s) 32, 235, 624
NlaIV GGNNCC 2 cut(s) 498, 618
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 2 cut(s) 194, 206
NsiI ATGCAT 1 cut(s) 147
PciSI GCTCTTC 1 cut(s) 461
PkrI GCNGC 5 cut(s) 343, 365, 488, 506, 567
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Psp6I CCWGG 1 cut(s) 524
PspGI CCWGG 1 cut(s) 524
PspN4I GGNNCC 2 cut(s) 498, 618
PsuI RGATCY 1 cut(s) 541
RsaI GTAC 5 cut(s) 57, 356, 386, 554, 646
RsaNI GTAC 5 cut(s) 56, 355, 385, 553, 645
SapI GCTCTTC 1 cut(s) 461
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 5 cut(s) 342, 364, 487, 505, 566
Sau3AI GATC 1 cut(s) 541
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 4 cut(s) 428, 494, 526, 539
SfaNI GCATC 1 cut(s) 154
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 2 cut(s) 601, 664
SsiI CCGC 9 cut(s) 47, 190, 318, 339, 342, 487, 504, 566, 637
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 4 cut(s) 426, 492, 524, 537
StyI CCWWGG 3 cut(s) 28, 96, 606
TaaI ACNGT 4 cut(s) 206, 501, 583, 634
TaiI ACGT 5 cut(s) 115, 278, 329, 519, 598
TaqI TCGA 1 cut(s) 423
TasI AATT 2 cut(s) 601, 664
TatI WGTACW 1 cut(s) 384
TauI GCSGC 4 cut(s) 344, 489, 507, 568
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 461
TseFI GTSAC 2 cut(s) 194, 206
TseI GCWGC 1 cut(s) 363
Tsp45I GTSAC 2 cut(s) 194, 206
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 479
XapI RAATTY 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.