pycom07g24830

Belongs to the MT-A70-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Reverse (-)
25374341 .. 25375581
1241 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGAAAGAATCAGTATCCTACTTTGCTAGGAGGACCGAAGCTGGTCAGCAGTCTGATGGGAAATCAGCACCGAGTGATGAAGAGTTGGCATATCTGCAGGATGACAGGACAAGGAGTGATGTATGGATCTGGATCGATCAGAATTCATGGAGGAAGGACTCTGATGCCCATGGAGGCAAAGGAAGAGGACAGAAAGGTTCCATTTCACAGCCTCCATATGGAAATGCGGAGCCAGGTTCCTTCAATAGAAATGCTTCGCAAGGGATGAAAGTGGGGAGAGTTGGTAGAGGAGGAAGGGGTAGGCTTACTGGAAGAGATAGCCAACCGGTAGGAATCCCACTTCCAATAATGGGATCGCCGTTTGGCCCTCTTGGAATGCCACCACCTGGACCTATGCAGCCACTTACACCTAGCATGTCACCTGTTCCAGGTCCACCGATGTCCCATGGTGTCTTCATTCCTTCATTTTCTCCCACCGTGTGGCCTGGGGCTCGGGGAGTTGACATGAATATGTTAGGTGTCCCACCTGGTCTCTCCTCAGTCCCTCCTGGATCATCAGGCCCAAGATTTCCTCCTAACAGGGGTACTCTGACAAACCCTGCCATGTTTTTTAACCAATCTGGCCATGGAAGGGGAGTTCCTCCAAACATATGTGGCCCTGGTTTTAATCCTGCAGGACCAATGGGACGAGGAACTCCGGCTGATAAAAATCAAGGTGGTTGGGTTCCTCATAAAAGTGGTGGACCTCCTGGTAAAGCTCCTTCCAGAGGAGAGCAGAATGATCACTCTCAAAATTTTGTCGATACTGGTATGCGACCCCAGAATTTCGTCAGGGAGCTAGAGCTTACAAATGTTGCGGAGGATTACCCCAAGCCGAGGGATCTTATACAGAAAAAGGATGAGATTGTGACCAACTCTGCTTCACATCCCATGTATTACGAGTGTGACCTGAATTTCTTATTCAGGAGGTCAAGGAATGTGCTATATCTTGCATGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MT-A70

Protein Analysis

334

Amino Acids

35.91

Weight (kDa)

9.59

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 386, 480
AciI CCGC 2 cut(s) 227, 857
AclWI GGATC 5 cut(s) 134, 140, 361, 559, 888
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 4 cut(s) 142, 793, 823, 952
AdeI CACNNNGTG 2 cut(s) 74, 480
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 9 cut(s) 218, 350, 386, 428, 480, 581, 631, 767, 876
AgeI ACCGGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
AloI GAACNNNNNNTCC 2 cut(s) 621, 653
AluBI AGCT 4 cut(s) 41, 758, 838, 844
AluI AGCT 4 cut(s) 41, 758, 838, 844
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 5 cut(s) 134, 140, 361, 559, 888
Ama87I CYCGRG 1 cut(s) 492
AoxI GGCC 5 cut(s) 364, 482, 559, 622, 655
ApeKI GCWGC 1 cut(s) 397
ApoI RAATTY 4 cut(s) 142, 793, 823, 952
AsiGI ACCGGT 1 cut(s) 325
Asp700I GAANNNNTTC 1 cut(s) 253
AspS9I GGNCC 8 cut(s) 33, 365, 389, 431, 560, 656, 677, 743
AsuHPI GGTGA 1 cut(s) 411
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 5 cut(s) 33, 389, 431, 677, 743
BalI TGGCCA 1 cut(s) 624
BanII GRGCYC 1 cut(s) 493
BarI GAAGNNNNNNTAC 2 cut(s) 745, 777
BbsI GAAGAC 1 cut(s) 445
BbvI GCAGC 1 cut(s) 409
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 781
BcoDI GTCTC 1 cut(s) 536
BfaI CTAG 4 cut(s) 27, 411, 839, 1000
BfmI CTRYAG 2 cut(s) 95, 672
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
Bme1390I CCNGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
Bme18I GGWCC 5 cut(s) 33, 389, 431, 677, 743
BmeT110I CYCGRG 1 cut(s) 492
BmgT120I GGNCC 8 cut(s) 33, 365, 389, 431, 560, 656, 677, 743
BmiI GGNNCC 4 cut(s) 199, 231, 238, 726
BmrFI CCNGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 445
Bsa29I ATCGAT 1 cut(s) 135
BsaBI GATNNNNATC 2 cut(s) 131, 708
BsaI GGTCTC 1 cut(s) 536
BsaJI CCNNGG 6 cut(s) 169, 445, 485, 625, 658, 875
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 9 cut(s) 218, 350, 386, 428, 480, 581, 631, 767, 876
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 2 cut(s) 313, 811
Bse8I GATNNNNATC 2 cut(s) 131, 708
BseBI CCWGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 6 cut(s) 169, 445, 485, 625, 658, 875
BseGI GGATG 4 cut(s) 106, 270, 904, 925
BseJI GATNNNNATC 2 cut(s) 131, 708
BseLI CCNNNNNNNGG 9 cut(s) 218, 350, 386, 428, 480, 581, 631, 767, 876
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 2 cut(s) 313, 811
BseRI GAGGAG 3 cut(s) 303, 526, 783
BseXI GCAGC 1 cut(s) 409
BshFI GGCC 5 cut(s) 366, 484, 561, 624, 657
BshTI ACCGGT 1 cut(s) 325
BshVI ATCGAT 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 492
BsiSI CCGG 2 cut(s) 326, 698
BslFI GGGAC 4 cut(s) 427, 506, 527, 699
BslI CCNNNNNNNGG 9 cut(s) 218, 350, 386, 428, 480, 581, 631, 767, 876
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 4 cut(s) 427, 506, 527, 699
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 5 cut(s) 366, 484, 561, 624, 657
Bso31I GGTCTC 1 cut(s) 536
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 493
Bsp143I GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
Bsp19I CCATGG 3 cut(s) 169, 445, 625
BspACI CCGC 2 cut(s) 227, 857
BspANI GGCC 5 cut(s) 366, 484, 561, 624, 657
BspCNI CTCAG 1 cut(s) 551
BspDI ATCGAT 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 199, 231, 238, 726
BspMAI CTGCAG 2 cut(s) 99, 676
BspPI GGATC 5 cut(s) 134, 140, 361, 559, 888
BspTNI GGTCTC 1 cut(s) 536
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 2 cut(s) 313, 811
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 6 cut(s) 169, 445, 485, 625, 658, 875
BssMI GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
BssT1I CCWWGG 3 cut(s) 169, 445, 625
Bst2UI CCWGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 3 cut(s) 75, 178, 307
BstDEI CTNAG 1 cut(s) 538
BstDSI CCRYGG 3 cut(s) 169, 445, 625
BstENI CCTNNNNNAGG 2 cut(s) 426, 765
BstF5I GGATG 4 cut(s) 106, 270, 904, 925
BstKTI GATC 7 cut(s) 129, 135, 139, 356, 554, 784, 883
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
BstNI CCWGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
BstSFI CTRYAG 2 cut(s) 95, 672
BstV1I GCAGC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 445
BstX2I RGATCY 2 cut(s) 126, 880
BstYI RGATCY 2 cut(s) 126, 880
Bsu15I ATCGAT 1 cut(s) 135
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 5 cut(s) 366, 484, 561, 624, 657
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 3 cut(s) 169, 445, 625
BtsCI GGATG 4 cut(s) 106, 270, 904, 925
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 8 cut(s) 33, 365, 389, 431, 560, 656, 677, 743
ClaI ATCGAT 1 cut(s) 135
CsiI ACCWGGT 1 cut(s) 526
Csp6I GTAC 1 cut(s) 585
CspAI ACCGGT 1 cut(s) 325
CviAII CATG 9 cut(s) 147, 170, 415, 446, 505, 604, 626, 931, 993
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 1 cut(s) 538
DpnI GATC 7 cut(s) 128, 134, 138, 355, 553, 783, 882
DpnII GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
DraIII CACNNNGTG 2 cut(s) 74, 480
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 3 cut(s) 75, 178, 307
EarI CTCTTC 3 cut(s) 75, 178, 307
Eco130I CCWWGG 3 cut(s) 169, 445, 625
Eco24I GRGCYC 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 536
Eco47I GGWCC 5 cut(s) 33, 389, 431, 677, 743
Eco88I CYCGRG 1 cut(s) 492
EcoNI CCTNNNNNAGG 2 cut(s) 426, 765
EcoRI GAATTC 1 cut(s) 142
EcoRII CCWGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
EcoT14I CCWWGG 3 cut(s) 169, 445, 625
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 3 cut(s) 169, 445, 625
FaeI CATG 9 cut(s) 150, 173, 418, 449, 508, 607, 629, 934, 996
FaqI GGGAC 4 cut(s) 427, 506, 527, 699
FatI CATG 9 cut(s) 146, 169, 414, 445, 504, 603, 625, 930, 992
FauNDI CATATG 2 cut(s) 217, 650
FbaI TGATCA 1 cut(s) 781
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 4 cut(s) 113, 277, 911, 912
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 398
FspBI CTAG 4 cut(s) 27, 411, 839, 1000
GluI GCNGC 1 cut(s) 398
HaeIII GGCC 5 cut(s) 366, 484, 561, 624, 657
HapII CCGG 2 cut(s) 326, 698
Hin1II CATG 9 cut(s) 150, 173, 418, 449, 508, 607, 629, 934, 996
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 3 cut(s) 8, 158, 333
HpaII CCGG 2 cut(s) 326, 698
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 3 cut(s) 434, 502, 743
Hpy188I TCNGA 4 cut(s) 55, 141, 163, 591
Hpy188III TCNNGA 3 cut(s) 130, 765, 964
Hpy8I GTNNAC 3 cut(s) 434, 502, 743
HpyAV CCTTC 6 cut(s) 148, 250, 288, 471, 624, 771
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4V TGCA 4 cut(s) 97, 397, 674, 992
HpyF3I CTNAG 1 cut(s) 538
Hsp92II CATG 9 cut(s) 150, 173, 418, 449, 508, 607, 629, 934, 996
Ksp22I TGATCA 1 cut(s) 781
Kzo9I GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
LmnI GCTCC 3 cut(s) 229, 763, 835
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 154
MabI ACCWGGT 1 cut(s) 526
MaeI CTAG 4 cut(s) 27, 411, 839, 1000
MaeIII GTNAC 3 cut(s) 417, 907, 944
MalI GATC 7 cut(s) 128, 134, 138, 355, 553, 783, 882
MboI GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
MboII GAAGA 4 cut(s) 92, 195, 324, 445
MflI RGATCY 2 cut(s) 126, 880
MhlI GDGCHC 1 cut(s) 493
MlsI TGGCCA 1 cut(s) 624
MluCI AATT 4 cut(s) 142, 793, 823, 952
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 1 cut(s) 152
Mox20I TGGCCA 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 253
MscI TGGCCA 1 cut(s) 624
MseI TTAA 2 cut(s) 612, 666
MslI CAYNNNNRTG 2 cut(s) 509, 735
Msp20I TGGCCA 1 cut(s) 624
MspI CCGG 2 cut(s) 326, 698
MspR9I CCNGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
NcoI CCATGG 3 cut(s) 169, 445, 625
NdeI CATATG 2 cut(s) 217, 650
NdeII GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
NlaIII CATG 9 cut(s) 150, 173, 418, 449, 508, 607, 629, 934, 996
NlaIV GGNNCC 4 cut(s) 199, 231, 238, 726
NmeAIII GCCGAG 1 cut(s) 900
NmuCI GTSAC 3 cut(s) 417, 907, 944
NspI RCATGY 1 cut(s) 418
PctI GAATGC 1 cut(s) 381
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 2 cut(s) 8, 333
PflMI CCANNNNNTGG 2 cut(s) 386, 480
PfoI TCCNGGA 1 cut(s) 547
PinAI ACCGGT 1 cut(s) 325
PkrI GCNGC 1 cut(s) 399
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp6I CCWGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
PspGI CCWGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
PspN4I GGNNCC 4 cut(s) 199, 231, 238, 726
PspPI GGNCC 8 cut(s) 33, 365, 389, 431, 560, 656, 677, 743
PstI CTGCAG 2 cut(s) 99, 676
PsuI RGATCY 2 cut(s) 126, 880
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 2 cut(s) 509, 735
SaqAI TTAA 2 cut(s) 612, 666
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 7 cut(s) 126, 132, 136, 353, 551, 781, 880
Sau96I GGNCC 8 cut(s) 33, 365, 389, 431, 560, 656, 677, 743
SbfI CCTGCAGG 1 cut(s) 676
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 8 cut(s) 234, 387, 429, 486, 528, 549, 660, 750
SdaI CCTGCAGG 1 cut(s) 676
SduI GDGCHC 1 cut(s) 493
SexAI ACCWGGT 1 cut(s) 526
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 2 cut(s) 95, 672
SinI GGWCC 5 cut(s) 33, 389, 431, 677, 743
SmiMI CAYNNNNRTG 2 cut(s) 509, 735
Sse8387I CCTGCAGG 1 cut(s) 676
Sse9I AATT 4 cut(s) 142, 793, 823, 952
SsiI CCGC 2 cut(s) 227, 857
SspMI CTAG 4 cut(s) 27, 411, 839, 1000
StyD4I CCNGG 8 cut(s) 232, 385, 427, 484, 526, 547, 658, 748
StyI CCWWGG 3 cut(s) 169, 445, 625
TaaI ACNGT 1 cut(s) 478
TaqI TCGA 2 cut(s) 135, 801
TaqII GACCGA 1 cut(s) 50
TasI AATT 4 cut(s) 142, 793, 823, 952
TfiI GAWTC 2 cut(s) 8, 333
Tru1I TTAA 2 cut(s) 612, 666
Tru9I TTAA 2 cut(s) 612, 666
TseFI GTSAC 3 cut(s) 417, 907, 944
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 3 cut(s) 417, 907, 944
TspDTI ATGAA 7 cut(s) 17, 93, 135, 281, 445, 453, 521
Van91I CCANNNNNTGG 2 cut(s) 386, 480
VpaK11BI GGWCC 5 cut(s) 33, 389, 431, 677, 743
XagI CCTNNNNNAGG 2 cut(s) 426, 765
XapI RAATTY 4 cut(s) 142, 793, 823, 952
XceI RCATGY 1 cut(s) 418
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 4 cut(s) 27, 411, 839, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.