pycom07g25780

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Reverse (-)
25972634 .. 25972903
270 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 270 bp
ATGGACTACGATTACAGAACCCGGTCCGGTCAAATGCCGAACCACCGTCCCGCCACGTCATCAGCGCCGTCTAGCCATCCGATGTACGGACCCCCGTTGTCGTCGTCCATGTACCCGCGGGTGGGTCAACAGAGCCACGCGGCGGTTCCGCCCCCGTACGGCGGTAACGCAGGCCGTCCTCTTCCCCACCACCAAACGGCAAACCCCCCTTCGTCTTCCTCCTGTAAGTTGAATCCAACCGATCATCTAATTTTTCCCCAAATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

90

Amino Acids

9.63

Weight (kDa)

9.43

Isoelectric Point (pI)

77.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 118, 140
AciI CCGC 7 cut(s) 51, 116, 118, 140, 143, 149, 162
AfaI GTAC 3 cut(s) 86, 113, 158
AfiI CCNNNNNNNGG 7 cut(s) 86, 121, 122, 142, 158, 161, 196
AgsI TTSAA 1 cut(s) 232
AjiI CACGTC 1 cut(s) 57
AoxI GGCC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 24, 89
AsuC2I CCSGG 1 cut(s) 22
AvaII GGWCC 2 cut(s) 24, 89
BbsI GAAGAC 1 cut(s) 207
BccI CCATC 1 cut(s) 84
BceAI ACGGC 4 cut(s) 52, 159, 175, 213
BcnI CCSGG 1 cut(s) 22
BfaI CTAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 22
Bme18I GGWCC 2 cut(s) 24, 89
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 24, 89
BmiI GGNNCC 2 cut(s) 91, 147
BmrFI CCNGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 22
BsaJI CCNNGG 1 cut(s) 116
BsaWI WCCGGW 1 cut(s) 26
Bsc4I CCNNNNNNNGG 7 cut(s) 86, 121, 122, 142, 158, 161, 196
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 76
BseLI CCNNNNNNNGG 7 cut(s) 86, 121, 122, 142, 158, 161, 196
Bsh1236I CGCG 2 cut(s) 118, 140
BshFI GGCC 1 cut(s) 174
BsiSI CCGG 2 cut(s) 22, 27
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 7 cut(s) 86, 121, 122, 142, 158, 161, 196
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 1 cut(s) 174
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 7 cut(s) 51, 116, 118, 140, 143, 149, 162
BspANI GGCC 1 cut(s) 174
BspFNI CGCG 2 cut(s) 118, 140
BspLI GGNNCC 2 cut(s) 91, 147
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 47
Bst6I CTCTTC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 172
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 1 cut(s) 76
BstFNI CGCG 2 cut(s) 118, 140
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 20
BstUI CGCG 2 cut(s) 118, 140
BstV2I GAAGAC 1 cut(s) 207
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 116
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 2 cut(s) 24, 89
Cfr42I CCGCGG 1 cut(s) 119
CpoI CGGWCCG 1 cut(s) 24
Csp6I GTAC 3 cut(s) 85, 112, 157
CspI CGGWCCG 1 cut(s) 24
CviAII CATG 1 cut(s) 109
CviJI RGCY 3 cut(s) 75, 135, 174
CviKI_1 RGCY 3 cut(s) 75, 135, 174
CviQI GTAC 3 cut(s) 85, 112, 157
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
EciI GGCGGA 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 24, 89
FaeI CATG 1 cut(s) 112
FaiI YATR 1 cut(s) 110
FaqI GGGAC 1 cut(s) 33
FatI CATG 1 cut(s) 108
FauI CCCGC 3 cut(s) 58, 111, 123
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 1 cut(s) 72
GlaI GCGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 1 cut(s) 174
HapII CCGG 2 cut(s) 22, 27
HhaI GCGC 1 cut(s) 67
Hin1II CATG 1 cut(s) 112
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HinfI GANTC 1 cut(s) 232
HpaII CCGG 2 cut(s) 22, 27
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 81
Hpy8I GTNNAC 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 1 cut(s) 47
HpyCH4IV ACGT 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 1 cut(s) 112
HspAI GCGC 1 cut(s) 65
KspI CCGCGG 1 cut(s) 119
Kzo9I GATC 1 cut(s) 241
LpnPI CCDG 4 cut(s) 35, 40, 156, 235
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 164
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 2 cut(s) 173, 207
MluCI AATT 2 cut(s) 249, 265
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 2 cut(s) 189, 229
MseI TTAA 1 cut(s) 268
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 2 cut(s) 22, 27
MspR9I CCNGG 1 cut(s) 22
MvnI CGCG 2 cut(s) 118, 140
NciI CCSGG 1 cut(s) 22
NdeII GATC 1 cut(s) 241
NlaIII CATG 1 cut(s) 112
NlaIV GGNNCC 2 cut(s) 91, 147
PfeI GAWTC 1 cut(s) 232
Pfl23II CGTACG 1 cut(s) 156
PkrI GCNGC 1 cut(s) 142
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 91, 147
PspPI GGNCC 2 cut(s) 24, 89
RsaI GTAC 3 cut(s) 86, 113, 158
RsaNI GTAC 3 cut(s) 85, 112, 157
Rsr2I CGGWCCG 1 cut(s) 24
RsrII CGGWCCG 1 cut(s) 24
SacII CCGCGG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 268
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 2 cut(s) 24, 89
ScrFI CCNGG 1 cut(s) 22
SetI ASST 1 cut(s) 59
Sfr303I CCGCGG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 119
SinI GGWCC 2 cut(s) 24, 89
Sse9I AATT 2 cut(s) 249, 265
SsiI CCGC 7 cut(s) 51, 116, 118, 140, 143, 149, 162
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 47
TaiI ACGT 1 cut(s) 59
TasI AATT 2 cut(s) 249, 265
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 1 cut(s) 268
Tru9I TTAA 1 cut(s) 268
TspGWI ACGGA 1 cut(s) 102
VpaK11BI GGWCC 2 cut(s) 24, 89
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.