pycom07g25800

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
25988183 .. 25989796
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGGAAACGACGTCGTCTTATCGAAAATCCTTTATTCGTTGGTGACTTTCGGCGCCAAAAATATAAAAACAAAAATCCAAAGGAAAAGTAAAAGGAAGGAGCGAGCGATAATCGTAAAGAGGACGCCAGGTGGGACCCAGACAGACGGAATAGAATTTAAGCCAATCAACGGCAAGTCAACAACAGGTCGATTCCATAAGGAGGGCGGACCGCAGAAGCACTTTTATAAAAAGTCCTTTCCCCCATCGTCAAAAAATCAATCGCATTTCCCTACCTCTTCCGTTTCCGTTTCCGTTTCCGCTTCAATCGAATTCGATTCTCCCTCGTCTCCGTCTTCGTTTTCTCTCCCTTTAACTCTTCCAATCAGGTTTTCGCAGATGGCGTCCAAGCGGATCTTGAAGGAACTCAAAGATCTGCAGAAGGATCCTCCTACGTCCTGCAGCGCCGTACTCTTCCTGCACTGCATATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04013 ko04120 ko04141 ko04624 map04013 map04120 map04141 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

159

Amino Acids

17.54

Weight (kDa)

10.24

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AatII GACGTC 1 cut(s) 15
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 4 cut(s) 207, 212, 300, 391
AclWI GGATC 3 cut(s) 401, 419, 432
AcsI RAATTY 2 cut(s) 155, 311
AcyI GRCGYC 4 cut(s) 12, 54, 125, 383
AfaI GTAC 1 cut(s) 450
AfiI CCNNNNNNNGG 2 cut(s) 170, 202
AgsI TTSAA 2 cut(s) 306, 400
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 2 cut(s) 355, 387
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 3 cut(s) 401, 419, 432
ApeKI GCWGC 1 cut(s) 441
ApoI RAATTY 2 cut(s) 155, 311
AspLEI GCGC 2 cut(s) 56, 446
AspS9I GGNCC 2 cut(s) 135, 209
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 2 cut(s) 135, 209
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 253, 373
BceAI ACGGC 2 cut(s) 187, 431
BciT130I CCWGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 333
BfmI CTRYAG 2 cut(s) 416, 439
BfoI RGCGCY 2 cut(s) 57, 447
BglII AGATCT 1 cut(s) 412
BisI GCNGC 1 cut(s) 442
BlsI GCNGC 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 129
Bme18I GGWCC 2 cut(s) 135, 209
BmgT120I GGNCC 2 cut(s) 135, 209
BmiI GGNNCC 4 cut(s) 55, 136, 137, 426
BmrFI CCNGG 1 cut(s) 129
BpiI GAAGAC 1 cut(s) 327
BsaHI GRCGYC 4 cut(s) 12, 54, 125, 383
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 202
BseBI CCWGG 1 cut(s) 129
BseLI CCNNNNNNNGG 2 cut(s) 170, 202
BseXI GCAGC 1 cut(s) 453
BsgI GTGCAG 1 cut(s) 443
BshNI GGYRCC 1 cut(s) 53
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 2 cut(s) 170, 202
BsmAI GTCTC 1 cut(s) 333
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 393, 412, 424
BspACI CCGC 4 cut(s) 207, 212, 300, 391
BspLI GGNNCC 4 cut(s) 55, 136, 137, 426
BspMAI CTGCAG 2 cut(s) 420, 443
BspPI GGATC 3 cut(s) 401, 419, 432
BspT107I GGYRCC 1 cut(s) 53
BssMI GATC 3 cut(s) 393, 412, 424
BssNI GRCGYC 4 cut(s) 12, 54, 125, 383
Bst2UI CCWGG 1 cut(s) 129
Bst6I CTCTTC 3 cut(s) 283, 363, 458
BstACI GRCGYC 4 cut(s) 12, 54, 125, 383
BstC8I GCNNGC 1 cut(s) 105
BstH2I RGCGCY 2 cut(s) 57, 447
BstHHI GCGC 2 cut(s) 56, 446
BstKTI GATC 3 cut(s) 396, 415, 427
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 3 cut(s) 393, 412, 424
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 2 cut(s) 416, 439
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 3 cut(s) 393, 412, 424
BstYI RGATCY 3 cut(s) 393, 412, 424
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 105
CfoI GCGC 2 cut(s) 56, 446
Cfr13I GGNCC 2 cut(s) 135, 209
CpoI CGGWCCG 1 cut(s) 209
CseI GACGC 2 cut(s) 133, 372
Csp6I GTAC 1 cut(s) 449
CspI CGGWCCG 1 cut(s) 209
CviJI RGCY 1 cut(s) 163
CviKI_1 RGCY 1 cut(s) 163
CviQI GTAC 1 cut(s) 449
DinI GGCGCC 1 cut(s) 55
DpnI GATC 3 cut(s) 395, 414, 426
DpnII GATC 3 cut(s) 393, 412, 424
Eam1104I CTCTTC 3 cut(s) 283, 363, 458
EarI CTCTTC 3 cut(s) 283, 363, 458
EciI GGCGGA 1 cut(s) 222
Eco47I GGWCC 2 cut(s) 135, 209
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 127
EgeI GGCGCC 1 cut(s) 55
EheI GGCGCC 1 cut(s) 55
Esp3I CGTCTC 1 cut(s) 333
FaiI YATR 5 cut(s) 65, 198, 228, 467, 469
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 148
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 442
Fsp4HI GCNGC 1 cut(s) 442
GlaI GCGC 2 cut(s) 55, 445
GluI GCNGC 1 cut(s) 442
HaeII RGCGCY 2 cut(s) 57, 447
HgaI GACGC 2 cut(s) 133, 372
HhaI GCGC 2 cut(s) 56, 446
Hin1I GRCGYC 4 cut(s) 12, 54, 125, 383
Hin6I GCGC 2 cut(s) 54, 444
HinP1I GCGC 2 cut(s) 54, 444
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 2 cut(s) 192, 317
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 180
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 1 cut(s) 180
Hpy99I CGWCG 2 cut(s) 14, 17
HpyAV CCTTC 3 cut(s) 91, 394, 415
HpyCH4IV ACGT 2 cut(s) 12, 434
HpyCH4V TGCA 4 cut(s) 418, 441, 460, 465
HpySE526I ACGT 2 cut(s) 12, 434
Hsp92I GRCGYC 4 cut(s) 12, 54, 125, 383
HspAI GCGC 2 cut(s) 54, 444
KasI GGCGCC 1 cut(s) 53
KflI GGGWCCC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 393, 412, 424
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 7 cut(s) 114, 141, 152, 171, 352, 451, 470
Lsp1109I GCAGC 1 cut(s) 453
MaeII ACGT 2 cut(s) 12, 434
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 395, 414, 426
MboI GATC 3 cut(s) 393, 412, 424
MboII GAAGA 4 cut(s) 270, 327, 350, 445
MflI RGATCY 3 cut(s) 393, 412, 424
MluCI AATT 2 cut(s) 155, 311
Mly113I GGCGCC 1 cut(s) 54
MnlI CCTC 5 cut(s) 114, 196, 286, 334, 438
MseI TTAA 2 cut(s) 159, 353
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
NarI GGCGCC 1 cut(s) 54
NdeI CATATG 1 cut(s) 467
NdeII GATC 3 cut(s) 393, 412, 424
NlaIV GGNNCC 4 cut(s) 55, 136, 137, 426
NmuCI GTSAC 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PfeI GAWTC 2 cut(s) 192, 317
PflFI GACNNNGTC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 443
PluTI GGCGCC 1 cut(s) 57
PpuMI RGGWCCY 1 cut(s) 135
PsiI TTATAA 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PspN4I GGNNCC 4 cut(s) 55, 136, 137, 426
PspPI GGNCC 2 cut(s) 135, 209
PspPPI RGGWCCY 1 cut(s) 135
PstI CTGCAG 2 cut(s) 420, 443
PsuI RGATCY 3 cut(s) 393, 412, 424
PsyI GACNNNGTC 1 cut(s) 14
RsaI GTAC 1 cut(s) 450
RsaNI GTAC 1 cut(s) 449
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SaqAI TTAA 2 cut(s) 159, 353
SatI GCNGC 1 cut(s) 442
Sau3AI GATC 3 cut(s) 393, 412, 424
Sau96I GGNCC 2 cut(s) 135, 209
ScrFI CCNGG 1 cut(s) 129
SetI ASST 6 cut(s) 15, 133, 190, 278, 371, 437
SfcI CTRYAG 2 cut(s) 416, 439
SfoI GGCGCC 1 cut(s) 55
SinI GGWCC 2 cut(s) 135, 209
Sse9I AATT 2 cut(s) 155, 311
SsiI CCGC 4 cut(s) 207, 212, 300, 391
SspDI GGCGCC 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 127
TaiI ACGT 2 cut(s) 15, 437
TaqI TCGA 4 cut(s) 23, 190, 309, 315
TasI AATT 2 cut(s) 155, 311
TfiI GAWTC 2 cut(s) 192, 317
Tru1I TTAA 2 cut(s) 159, 353
Tru9I TTAA 2 cut(s) 159, 353
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 441
Tsp45I GTSAC 1 cut(s) 43
TspGWI ACGGA 5 cut(s) 162, 271, 277, 283, 321
TspRI CASTG 1 cut(s) 467
Tth111I GACNNNGTC 1 cut(s) 14
VpaK11BI GGWCC 2 cut(s) 135, 209
XapI RAATTY 2 cut(s) 155, 311
ZraI GACGTC 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.