pycom07g27610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
N/A
Physical Location & Seq
Forward (+)
27266556 .. 27267980
1425 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGCAACAATACAAGGTGGATCTAGTATTAAAATTTAAAAGATCTAGATCATTTGATTTGATAGTCTTGATAGAAGAAACTCGGAGGGGATCTCTTCTCCTTTTTATCAGTTTGGTAACTCCAACGTCAACTCCTCCACCGGTCCACCCCCACCTTCTCTCTCCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCCCAGCAATTTGGATAGCGAGAGAGACAGTCCGAGTCTTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGTGAGGAGTAGGTGGTGGTGTTGCTGGGTTAGCGTTGAAGCACAGCGCGCGTGTTCTTCGTTTTTAACTAGGAAGCACAGACTTTTTGCAATGGAGGTGGCTGGCAATTCTGTGGTAGCAGGACCTACGAATGTTGTTTATCTGTCGACTATTTTGGGCCGTGATGTGTCAAATCCTGTTCACAAATGTGACTGGAAATGTCAGAACGAGCACGTCTGCGCGAATATGTACCTCTGCAAGCTAACTGGAGCCACTCACATCTGTGACAAAAACTGTGATCAGATGGATCCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

21.42

Weight (kDa)

9.05

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 413
AccII CGCG 3 cut(s) 315, 317, 488
AclWI GGATC 4 cut(s) 27, 97, 548, 561
AcsI RAATTY 1 cut(s) 32
AfaI GTAC 1 cut(s) 497
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 1 cut(s) 305
AjiI CACGTC 1 cut(s) 481
AleI CACNNNNGTG 2 cut(s) 453, 528
AluBI AGCT 1 cut(s) 508
AluI AGCT 1 cut(s) 508
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 4 cut(s) 27, 97, 548, 561
AoxI GGCC 1 cut(s) 424
ApoI RAATTY 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 139
AspLEI GCGC 3 cut(s) 315, 317, 488
AspS9I GGNCC 3 cut(s) 142, 389, 424
AsuHPI GGTGA 1 cut(s) 280
AvaII GGWCC 2 cut(s) 142, 389
BamHI GGATCC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 480
BccI CCATC 1 cut(s) 544
BceAI ACGGC 1 cut(s) 411
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 3 cut(s) 23, 45, 336
BglII AGATCT 1 cut(s) 41
Bme18I GGWCC 2 cut(s) 142, 389
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 3 cut(s) 142, 389, 424
BmiI GGNNCC 2 cut(s) 517, 555
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BpmI CTGGAG 1 cut(s) 534
BsaBI GATNNNNATC 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 115, 145
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 2 cut(s) 464, 517
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 46
BseJI GATNNNNATC 1 cut(s) 46
BseMI GCAATG 1 cut(s) 363
BseNI ACTGG 2 cut(s) 464, 517
BsePI GCGCGC 1 cut(s) 313
BseRI GAGGAG 3 cut(s) 123, 153, 286
BseYI CCCAGC 2 cut(s) 199, 291
Bsh1236I CGCG 3 cut(s) 315, 317, 488
BshFI GGCC 1 cut(s) 426
BshTI ACCGGT 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 215
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 6 cut(s) 19, 41, 47, 89, 544, 553
BspANI GGCC 1 cut(s) 426
BspFNI CGCG 3 cut(s) 315, 317, 488
BspLI GGNNCC 2 cut(s) 517, 555
BspPI GGATC 4 cut(s) 27, 97, 548, 561
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 2 cut(s) 464, 517
BssAI RCCGGY 1 cut(s) 139
BssHII GCGCGC 1 cut(s) 313
BssMI GATC 6 cut(s) 19, 41, 47, 89, 544, 553
Bst4CI ACNGT 2 cut(s) 226, 542
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 3 cut(s) 315, 370, 506
BstFNI CGCG 3 cut(s) 315, 317, 488
BstHHI GCGC 3 cut(s) 315, 317, 488
BstKTI GATC 6 cut(s) 22, 44, 50, 92, 547, 556
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 6 cut(s) 19, 41, 47, 89, 544, 553
BstMWI GCNNNNNNNGC 2 cut(s) 297, 314
BstUI CGCG 3 cut(s) 315, 317, 488
BstX2I RGATCY 4 cut(s) 19, 41, 89, 553
BstXI CCANNNNNNTGG 1 cut(s) 207
BstYI RGATCY 4 cut(s) 19, 41, 89, 553
BsuRI GGCC 1 cut(s) 426
BtrI CACGTC 1 cut(s) 481
Cac8I GCNNGC 3 cut(s) 315, 370, 506
CfoI GCGC 3 cut(s) 315, 317, 488
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 3 cut(s) 142, 389, 424
Csp6I GTAC 1 cut(s) 496
CspAI ACCGGT 1 cut(s) 139
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviJI RGCY 4 cut(s) 368, 426, 508, 518
CviKI_1 RGCY 4 cut(s) 368, 426, 508, 518
CviQI GTAC 1 cut(s) 496
DpnI GATC 6 cut(s) 21, 43, 49, 91, 546, 555
DpnII GATC 6 cut(s) 19, 41, 47, 89, 544, 553
DraI TTTAAA 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco47I GGWCC 2 cut(s) 142, 389
EcoO109I RGGNCCY 1 cut(s) 389
FaiI YATR 2 cut(s) 494, 562
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 1 cut(s) 413
FspBI CTAG 3 cut(s) 23, 45, 336
GlaI GCGC 3 cut(s) 314, 316, 487
GsaI CCCAGC 2 cut(s) 203, 295
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 1 cut(s) 426
HapII CCGG 1 cut(s) 140
HhaI GCGC 3 cut(s) 315, 317, 488
Hin6I GCGC 3 cut(s) 313, 315, 486
HinP1I GCGC 3 cut(s) 313, 315, 486
HincII GTYRAC 2 cut(s) 129, 414
HindII GTYRAC 2 cut(s) 129, 414
HinfI GANTC 1 cut(s) 231
HpaII CCGG 1 cut(s) 140
HphI GGTGA 1 cut(s) 280
Hpy166II GTNNAC 4 cut(s) 129, 145, 414, 448
Hpy188I TCNGA 4 cut(s) 84, 230, 471, 549
Hpy188III TCNNGA 2 cut(s) 45, 67
Hpy8I GTNNAC 4 cut(s) 129, 145, 414, 448
HpyAV CCTTC 2 cut(s) 164, 259
HpyCH4III ACNGT 2 cut(s) 226, 542
HpyCH4IV ACGT 2 cut(s) 125, 480
HpyCH4V TGCA 3 cut(s) 4, 356, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 314
HpySE526I ACGT 2 cut(s) 125, 480
HspAI GCGC 3 cut(s) 313, 315, 486
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 6 cut(s) 19, 41, 47, 89, 544, 553
LmnI GCTCC 1 cut(s) 515
LpnPI CCDG 8 cut(s) 153, 213, 277, 354, 372, 445, 456, 498
MaeI CTAG 3 cut(s) 23, 45, 336
MaeII ACGT 2 cut(s) 125, 480
MaeIII GTNAC 3 cut(s) 115, 455, 530
MalI GATC 6 cut(s) 21, 43, 49, 91, 546, 555
MboI GATC 6 cut(s) 19, 41, 47, 89, 544, 553
MboII GAAGA 3 cut(s) 86, 86, 315
MflI RGATCY 4 cut(s) 19, 41, 89, 553
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 3 cut(s) 32, 204, 373
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 8 cut(s) 78, 144, 174, 178, 182, 264, 355, 509
MseI TTAA 3 cut(s) 29, 36, 332
MslI CAYNNNNRTG 2 cut(s) 453, 528
MspI CCGG 1 cut(s) 140
MvnI CGCG 3 cut(s) 315, 317, 488
MwoI GCNNNNNNNGC 2 cut(s) 297, 314
NdeII GATC 6 cut(s) 19, 41, 47, 89, 544, 553
NlaIV GGNNCC 2 cut(s) 517, 555
NmuCI GTSAC 2 cut(s) 455, 530
OliI CACNNNNGTG 2 cut(s) 453, 528
PauI GCGCGC 1 cut(s) 313
PinAI ACCGGT 1 cut(s) 139
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PpuMI RGGWCCY 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 389
PspFI CCCAGC 2 cut(s) 199, 291
PspN4I GGNNCC 2 cut(s) 517, 555
PspPI GGNCC 3 cut(s) 142, 389, 424
PspPPI RGGWCCY 1 cut(s) 389
PsuI RGATCY 4 cut(s) 19, 41, 89, 553
PteI GCGCGC 1 cut(s) 313
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 2 cut(s) 453, 528
SalI GTCGAC 1 cut(s) 412
SaqAI TTAA 3 cut(s) 29, 36, 332
Sau3AI GATC 6 cut(s) 19, 41, 47, 89, 544, 553
Sau96I GGNCC 3 cut(s) 142, 389, 424
SchI GAGTC 1 cut(s) 240
SduI GDGCHC 1 cut(s) 480
SinI GGWCC 2 cut(s) 142, 389
SmiMI CAYNNNNRTG 2 cut(s) 453, 528
Sse9I AATT 3 cut(s) 32, 204, 373
SspMI CTAG 3 cut(s) 23, 45, 336
TaaI ACNGT 2 cut(s) 226, 542
TaiI ACGT 2 cut(s) 128, 483
TaqI TCGA 1 cut(s) 413
TasI AATT 3 cut(s) 32, 204, 373
Tru1I TTAA 3 cut(s) 29, 36, 332
Tru9I TTAA 3 cut(s) 29, 36, 332
TseFI GTSAC 2 cut(s) 455, 530
Tsp45I GTSAC 2 cut(s) 455, 530
VpaK11BI GGWCC 2 cut(s) 142, 389
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 3 cut(s) 23, 45, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.