pycom08g02750

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Reverse (-)
2001104 .. 2002902
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGCCGACCTCTCCAGCTCAGCCTCTCGCCGCCAGCTTTCCGTCACCACTCCGTCCCAGATCTCCAAAGGTGGACAAGGTTCTGAGAATCTTATTCCCCTTTCACCGCAATGGCTTCTGCCTAAGCCGGGGGAGAGTAAGACAGGAATGTTAACTGGGGAAAAACCTCCCAGCCCAAATCCATCCTTTTGCAGTCGCTTGGATACCATGAAATCATCAGGTAATGGTGATGAAATTCGTGATACCCAGAAGAAGGATGTTTTCAGGCCATCTTTGATGGATATGGAAGCTGGTGGTCATCGTGAACGCTGGCGTGATGAAGAAAGAGACACCAACTCCTCAGGACGCAAAGATCGTTGGAGGGATGGTGATAAAGAAATTGGTGACACTCGCAAGATGGATCGATGGATGGAAAATTCATCTGCAAAGCAATTTGGAGAAGCACGTCGTGCCCCACTTGAGCGGTGGACTGACTCAAGTAACAAGGAATCCAATTATGAGCAACGGCGAGAGAACAAATGGAACATGCGCTGGGGGCCTAATGACAAGGAGGCAGAAGGTTTTCAAATTCGTGATACCCAGAAGATGGATGTTTTCAGCCCATCTTTGATGGATATGGAAGGAAGCTGGTGGTCGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

214

Amino Acids

24.46

Weight (kDa)

6.66

Isoelectric Point (pI)

61.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 586
AccBSI CCGCTC 1 cut(s) 463
AciI CCGC 3 cut(s) 31, 107, 463
AclWI GGATC 1 cut(s) 408
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 234, 415, 567
AdeI CACNNNGTG 1 cut(s) 449
AfiI CCNNNNNNNGG 3 cut(s) 128, 253, 586
AgsI TTSAA 1 cut(s) 566
AjiI CACGTC 1 cut(s) 446
AluBI AGCT 4 cut(s) 18, 37, 290, 627
AluI AGCT 4 cut(s) 18, 37, 290, 627
Alw26I GTCTC 1 cut(s) 321
AlwI GGATC 1 cut(s) 408
AoxI GGCC 3 cut(s) 3, 266, 536
ApoI RAATTY 3 cut(s) 234, 415, 567
Asp700I GAANNNNTTC 1 cut(s) 561
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 1 cut(s) 536
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 5 cut(s) 37, 96, 239, 380, 395
AxyI CCTNAGG 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 454
BceAI ACGGC 1 cut(s) 521
BciVI GTATCC 1 cut(s) 196
BcnI CCSGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 196
BglII AGATCT 1 cut(s) 60
BisI GCNGC 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 129
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 1 cut(s) 537
BmrFI CCNGG 1 cut(s) 129
BmrI ACTGGG 1 cut(s) 165
BmuI ACTGGG 1 cut(s) 165
Bpu10I CCTNAGC 1 cut(s) 123
Bpu1102I GCTNAGC 1 cut(s) 19
BpuEI CTTGAG 2 cut(s) 460, 479
BpuMI CCSGG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 352, 382
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 253, 586
Bse1I ACTGG 1 cut(s) 160
Bse21I CCTNAGG 1 cut(s) 340
Bse3DI GCAATG 1 cut(s) 116
BseCI ATCGAT 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 128
BseGI GGATG 5 cut(s) 182, 262, 370, 414, 595
BseLI CCNNNNNNNGG 3 cut(s) 128, 253, 586
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 33, 75, 354
BseNI ACTGG 1 cut(s) 160
BseRI GAGGAG 1 cut(s) 328
BseSI GKGCMC 1 cut(s) 454
BseYI CCCAGC 2 cut(s) 170, 531
BshFI GGCC 3 cut(s) 5, 268, 538
BshVI ATCGAT 1 cut(s) 403
BsiSI CCGG 1 cut(s) 128
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 3 cut(s) 128, 253, 586
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 40
BsnI GGCC 3 cut(s) 5, 268, 538
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 3 cut(s) 60, 352, 400
Bsp1720I GCTNAGC 1 cut(s) 19
BspACI CCGC 3 cut(s) 31, 107, 463
BspANI GGCC 3 cut(s) 5, 268, 538
BspCNI CTCAG 3 cut(s) 32, 76, 353
BspDI ATCGAT 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 537
BspPI GGATC 1 cut(s) 408
BsrBI CCGCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 116
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 128
BssMI GATC 3 cut(s) 60, 352, 400
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 35, 311
BstDEI CTNAG 4 cut(s) 19, 84, 123, 340
BstF5I GGATG 5 cut(s) 182, 262, 370, 414, 595
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 63, 355, 403
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 60, 352, 400
BstMWI GCNNNNNNNGC 2 cut(s) 449, 535
BstNSI RCATGY 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 127
BstSLI GKGCMC 1 cut(s) 454
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
Bsu15I ATCGAT 1 cut(s) 403
Bsu36I CCTNAGG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 196
BsuRI GGCC 3 cut(s) 5, 268, 538
BsuTUI ATCGAT 1 cut(s) 403
BtrI CACGTC 1 cut(s) 446
BtsCI GGATG 5 cut(s) 182, 262, 370, 414, 595
Cac8I GCNNGC 2 cut(s) 35, 311
CfoI GCGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 536
ClaI ATCGAT 1 cut(s) 403
CseI GACGC 1 cut(s) 354
CviAII CATG 2 cut(s) 208, 526
DdeI CTNAG 4 cut(s) 19, 84, 123, 340
DpnI GATC 3 cut(s) 62, 354, 402
DpnII GATC 3 cut(s) 60, 352, 400
DraIII CACNNNGTG 1 cut(s) 449
EaeI YGGCCR 1 cut(s) 3
Eco81I CCTNAGG 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 536
FaeI CATG 2 cut(s) 211, 529
FaiI YATR 5 cut(s) 209, 284, 498, 527, 617
FaqI GGGAC 1 cut(s) 40
FatI CATG 2 cut(s) 207, 525
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 5 cut(s) 169, 269, 377, 421, 602
Fsp4HI GCNGC 1 cut(s) 31
GlaI GCGC 1 cut(s) 530
GluI GCNGC 1 cut(s) 31
GsaI CCCAGC 2 cut(s) 174, 535
HaeIII GGCC 3 cut(s) 5, 268, 538
HapII CCGG 1 cut(s) 128
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 531
Hin1II CATG 2 cut(s) 211, 529
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 3 cut(s) 88, 473, 488
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 1 cut(s) 128
HphI GGTGA 5 cut(s) 37, 96, 239, 380, 395
Hpy166II GTNNAC 4 cut(s) 74, 153, 305, 468
Hpy188I TCNGA 1 cut(s) 85
Hpy188III TCNNGA 5 cut(s) 239, 302, 342, 572, 639
Hpy8I GTNNAC 4 cut(s) 74, 153, 305, 468
Hpy99I CGWCG 2 cut(s) 450, 640
HpyAV CCTTC 3 cut(s) 247, 551, 614
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 2 cut(s) 192, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 535
HpyF3I CTNAG 4 cut(s) 19, 84, 123, 340
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 2 cut(s) 211, 529
HspAI GCGC 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 153
Kzo9I GATC 3 cut(s) 60, 352, 400
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 43, 383, 479
MalI GATC 3 cut(s) 62, 354, 402
MbiI CCGCTC 1 cut(s) 463
MboI GATC 3 cut(s) 60, 352, 400
MboII GAAGA 3 cut(s) 262, 332, 595
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 6 cut(s) 234, 378, 415, 431, 493, 567
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 6 cut(s) 20, 34, 177, 349, 354, 544
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 1 cut(s) 109
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 129
MwoI GCNNNNNNNGC 2 cut(s) 449, 535
NciI CCSGG 1 cut(s) 129
NdeII GATC 3 cut(s) 60, 352, 400
NlaIII CATG 2 cut(s) 211, 529
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 2 cut(s) 43, 383
NspI RCATGY 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 2 cut(s) 88, 488
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 1 cut(s) 32
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PspFI CCCAGC 2 cut(s) 170, 531
PspN4I GGNNCC 1 cut(s) 537
PspPI GGNCC 1 cut(s) 536
PsuI RGATCY 1 cut(s) 60
RseI CAYNNNNRTG 1 cut(s) 109
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 3 cut(s) 60, 352, 400
Sau96I GGNCC 1 cut(s) 536
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 458, 475
SmoI CTYRAG 2 cut(s) 458, 475
Sse9I AATT 6 cut(s) 234, 378, 415, 431, 493, 567
SsiI CCGC 3 cut(s) 31, 107, 463
StyD4I CCNGG 1 cut(s) 127
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 403
TasI AATT 6 cut(s) 234, 378, 415, 431, 493, 567
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 88, 488
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TseFI GTSAC 2 cut(s) 43, 383
Tsp45I GTSAC 2 cut(s) 43, 383
TspDTI ATGAA 4 cut(s) 224, 246, 333, 408
TspGWI ACGGA 2 cut(s) 31, 42
Van91I CCANNNNNTGG 1 cut(s) 586
XapI RAATTY 3 cut(s) 234, 415, 567
XceI RCATGY 1 cut(s) 529
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmnI GAANNNNTTC 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.