pycom08g03680

G-protein coupled receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Forward (+)
2842574 .. 2844226
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
ATGCATCAAGCAACGTATAAATTTACTAAATTCATAGCACATCGGCAAATGAGAATTGCAACCTTTCGAATAGACACGCACACGGATTATCCACCACCACGTCATGTAACCAATCGCCAGCCCCAAAACGATGGCCACGAACACTCCGCCGTTAAACGACATGATCGCCAGCATAATCATGTACCCGATCGCCGCGTTGACTCCGAAAAGCACGCTCTCCGCCAGCCTCCCCGCCGAGAACCTACCTCCGGCGCCGCCGAGCTTGGCACTGAGCAGCGGGGAGGCCGCGCGCTTGATGCGCACGCGCAGGTCCTCGAGGTACTGGTAGAAGGCACGGACGAGAAGGCAGGCGAGCAGCGTGAGGGAGTAGCTCGTCCACGTGTCGGTTTTCCACGAGTCGAAGAGGAGCGTTACCTGCCGGCTCCAGTAGAAGGTCATGTGCATCATCTTCTTCGATTTCTAGAGAGAGAAAGTAGAAGCAGCGGATTTTTGGGGGATTTTTGGAGAACAAGACTTGCGAGAGACGGTGTTAATTGTTTGGTACTAGATGTGCGTTGGGATGTACCCTTGGAGTTGTTATTATTTACGGTTTTGTCTCCATCGGTTTCTAGACTATTTGGGCTTGATGGCAATTTTGTCCTAAGGAAAAAAAACCCAAAATGCAGTACAAGGATCGAAGATGGTAAAGAAAGTAAACGAAGGCCCAACGTAGTTTCTTCCCTTTCTCTCCAAAAGCGAGAGCAACTTGTCTGGATCGGATTTATCACTATTATGATTATTAGATCAGGAGGTCGCAAGATAGGAAATCAGCTTTATGCAAGTCCTCCTGTAAATGGCAAGTTTTGCTATTTTGATCTCATCAAGTGCCTTTTTAATAAGATCAAATCTTTTCAAGTTGGATTGTGGTATTATGAATGTAGTCTCACTTGTTCCAATCCAATATATTCAATCAAAACTTCTACATCAAAGGAGACTAGTTTTACACTAAAGAGAGAGGGAGTTCGACTAAGCCACACAACGGGTAGCCTACTTTGGTATCGAATTCTAAAGATCCACTTTGTATCTAATTGTCCAGAGCTCAAGGATCGGTTCGCTAGCCCACCTCACAAATCAAGGACTCTTATTTCTCTCTTTCACACAAACCAAAGGCACGAACTTCCTGTCCTTAGACTCGGAGCACTAAATTCTAGAGAGGAACCAACCTTATCTTCTTCTTGCTCTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0001101 GO:0003006 GO:0003674 GO:0004888 GO:0004930 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006082 GO:0006109 GO:0006520 GO:0006558 GO:0006570 GO:0006571 GO:0006725 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007186 GO:0007202 GO:0007275 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009094 GO:0009095 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009642 GO:0009645 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009738 GO:0009741 GO:0009742 GO:0009755 GO:0009785 GO:0009787 GO:0009788 GO:0009791 GO:0009845 GO:0009889 GO:0009908 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010154 GO:0010162 GO:0010231 GO:0010244 GO:0010431 GO:0010517 GO:0010518 GO:0010646 GO:0010647 GO:0010648 GO:0010675 GO:0010863 GO:0010919 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016053 GO:0017144 GO:0019220 GO:0019222 GO:0019438 GO:0019752 GO:0021700 GO:0022414 GO:0022611 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030522 GO:0031090 GO:0031224 GO:0031226 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0032960 GO:0033993 GO:0038023 GO:0042221 GO:0043085 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043255 GO:0043401 GO:0043436 GO:0044093 GO:0044214 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046394 GO:0048316 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048545 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051174 GO:0051336 GO:0051345 GO:0051716 GO:0060089 GO:0060191 GO:0060193 GO:0061458 GO:0062012 GO:0065007 GO:0065009 GO:0070887 GO:0071214 GO:0071215 GO:0071229 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071478 GO:0071482 GO:0071483 GO:0071495 GO:0071695 GO:0071704 GO:0071944 GO:0080090 GO:0089717 GO:0090351 GO:0090567 GO:0097305 GO:0097306 GO:0097437 GO:0098588 GO:0098805 GO:0104004 GO:1900274 GO:1901360 GO:1901362 GO:1901419 GO:1901420 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901700 GO:1901701 GO:1902221 GO:1902223 GO:1902930 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dicty_CAR

Protein Analysis

409

Amino Acids

46.85

Weight (kDa)

9.84

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 314
Acc16I TGCGCA 1 cut(s) 300
Acc36I ACCTGC 2 cut(s) 298, 423
AccB1I GGYRCC 1 cut(s) 251
AccII CGCG 4 cut(s) 195, 288, 290, 305
AciI CCGC 8 cut(s) 147, 193, 220, 232, 255, 277, 286, 483
AclWI GGATC 4 cut(s) 680, 761, 1045, 1092
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 4 cut(s) 20, 29, 1041, 1183
AcvI CACGTG 1 cut(s) 380
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 5 cut(s) 183, 321, 543, 564, 667
AfiI CCNNNNNNNGG 5 cut(s) 248, 383, 431, 833, 1018
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 2 cut(s) 893, 948
AhlI ACTAGT 1 cut(s) 974
AjiI CACGTC 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 1192, 1224
AloI GAACNNNNNNTCC 2 cut(s) 1146, 1178
AluBI AGCT 4 cut(s) 262, 371, 811, 1078
AluI AGCT 4 cut(s) 262, 371, 811, 1078
Alw21I GWGCWC 2 cut(s) 1080, 1180
Alw26I GTCTC 4 cut(s) 516, 600, 926, 965
AlwI GGATC 4 cut(s) 680, 761, 1045, 1092
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 3 cut(s) 133, 283, 701
ApeKI GCWGC 3 cut(s) 274, 355, 480
ApoI RAATTY 4 cut(s) 20, 29, 1041, 1183
ArsI GACNNNNNNTTYG 2 cut(s) 1146, 1178
AspLEI GCGC 5 cut(s) 254, 290, 292, 301, 307
AspS9I GGNCC 2 cut(s) 310, 702
AsuII TTCGAA 1 cut(s) 67
AsuNHI GCTAGC 1 cut(s) 1094
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 310
AxyI CCTNAGG 1 cut(s) 641
BaeI ACNNNNGTAYC 2 cut(s) 1019, 1052
BalI TGGCCA 1 cut(s) 135
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 1080
BauI CACGAG 1 cut(s) 393
BbrPI CACGTG 1 cut(s) 380
Bbv12I GWGCWC 2 cut(s) 1080, 1180
BbvI GCAGC 3 cut(s) 286, 367, 492
BccI CCATC 4 cut(s) 125, 607, 620, 674
BceAI ACGGC 1 cut(s) 134
BcoDI GTCTC 4 cut(s) 516, 600, 926, 965
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 6 cut(s) 461, 545, 609, 975, 1095, 1188
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 2 cut(s) 298, 423
BisI GCNGC 6 cut(s) 193, 255, 275, 286, 356, 481
BlsI GCNGC 6 cut(s) 194, 256, 276, 287, 357, 482
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 310, 702
BmiI GGNNCC 3 cut(s) 253, 423, 1197
BmsI GCATC 3 cut(s) 13, 286, 451
BmtI GCTAGC 1 cut(s) 1098
BpmI CTGGAG 1 cut(s) 408
Bpu14I TTCGAA 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 1064
BsaAI YACGTR 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 383, 431, 833, 1018
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 327, 425
Bse21I CCTNAGG 1 cut(s) 641
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 1 cut(s) 565
BseLI CCNNNNNNNGG 5 cut(s) 248, 383, 431, 833, 1018
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 2 cut(s) 327, 425
BsePI GCGCGC 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 3 cut(s) 286, 367, 492
Bsh1236I CGCG 4 cut(s) 195, 288, 290, 305
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 135, 285, 703
BshNI GGYRCC 1 cut(s) 251
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 1080, 1180
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 2 cut(s) 249, 419
BslI CCNNNNNNNGG 5 cut(s) 248, 383, 431, 833, 1018
BsmAI GTCTC 4 cut(s) 516, 600, 926, 965
BsmBI CGTCTC 1 cut(s) 516
BsnI GGCC 3 cut(s) 135, 285, 703
BsoBI CYCGRG 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 1080, 1180
Bsp143I GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
BspACI CCGC 8 cut(s) 147, 193, 220, 232, 255, 277, 286, 483
BspANI GGCC 3 cut(s) 135, 285, 703
BspCNI CTCAG 1 cut(s) 262
BspFNI CGCG 4 cut(s) 195, 288, 290, 305
BspLI GGNNCC 3 cut(s) 253, 423, 1197
BspMI ACCTGC 2 cut(s) 298, 423
BspOI GCTAGC 1 cut(s) 1098
BspPI GGATC 4 cut(s) 680, 761, 1045, 1092
BspT104I TTCGAA 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 2 cut(s) 327, 425
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 1 cut(s) 567
BssHII GCGCGC 1 cut(s) 288
BssMI GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 527, 589
Bst6I CTCTTC 1 cut(s) 396
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 843
BstBAI YACGTR 1 cut(s) 380
BstBI TTCGAA 1 cut(s) 67
BstDEI CTNAG 4 cut(s) 270, 641, 1007, 1166
BstF5I GGATG 1 cut(s) 565
BstFNI CGCG 4 cut(s) 195, 288, 290, 305
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 5 cut(s) 254, 290, 292, 301, 307
BstKTI GATC 9 cut(s) 166, 190, 675, 756, 785, 856, 882, 1053, 1087
BstMAI GTCTC 4 cut(s) 516, 600, 926, 965
BstMBI GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 4 cut(s) 296, 298, 415, 843
BstUI CGCG 4 cut(s) 195, 288, 290, 305
BstV1I GCAGC 3 cut(s) 286, 367, 492
BstX2I RGATCY 1 cut(s) 1050
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 1 cut(s) 1050
Bsu36I CCTNAGG 1 cut(s) 641
BsuRI GGCC 3 cut(s) 135, 285, 703
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 1 cut(s) 565
BtsIMutI CAGTG 1 cut(s) 267
BveI ACCTGC 2 cut(s) 298, 423
CfoI GCGC 5 cut(s) 254, 290, 292, 301, 307
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 2 cut(s) 310, 702
Csp6I GTAC 5 cut(s) 182, 320, 542, 563, 666
CviAII CATG 4 cut(s) 104, 161, 179, 437
CviQI GTAC 5 cut(s) 182, 320, 542, 563, 666
DdeI CTNAG 4 cut(s) 270, 641, 1007, 1166
DinI GGCGCC 1 cut(s) 253
DpnI GATC 9 cut(s) 165, 189, 674, 755, 784, 855, 881, 1052, 1086
DpnII GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
EaeI YGGCCR 1 cut(s) 133
Eam1104I CTCTTC 1 cut(s) 396
EarI CTCTTC 1 cut(s) 396
EciI GGCGGA 2 cut(s) 136, 209
Ecl136II GAGCTC 1 cut(s) 1078
Eco130I CCWWGG 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 1080
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 1078
Eco72I CACGTG 1 cut(s) 380
Eco81I CCTNAGG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 1078
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 1041
EcoT14I CCWWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1080
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 4 cut(s) 107, 164, 182, 440
FalI AAGNNNNNCTT 2 cut(s) 1040, 1072
FatI CATG 4 cut(s) 103, 160, 178, 436
FauI CCCGC 2 cut(s) 239, 270
Fnu4HI GCNGC 6 cut(s) 193, 255, 275, 286, 356, 481
FokI GGATG 1 cut(s) 572
FriOI GRGCYC 1 cut(s) 1080
Fsp4HI GCNGC 6 cut(s) 193, 255, 275, 286, 356, 481
FspAI RTGCGCAY 1 cut(s) 300
FspBI CTAG 6 cut(s) 461, 545, 609, 975, 1095, 1188
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 5 cut(s) 253, 289, 291, 300, 306
GluI GCNGC 6 cut(s) 193, 255, 275, 286, 356, 481
GsuI CTGGAG 1 cut(s) 408
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 3 cut(s) 135, 285, 703
HapII CCGG 2 cut(s) 249, 419
HhaI GCGC 5 cut(s) 254, 290, 292, 301, 307
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 107, 164, 182, 440
Hin6I GCGC 5 cut(s) 252, 288, 290, 299, 305
HinP1I GCGC 5 cut(s) 252, 288, 290, 299, 305
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 4 cut(s) 200, 396, 1117, 1170
HpaII CCGG 2 cut(s) 249, 419
Hpy166II GTNNAC 3 cut(s) 199, 377, 695
Hpy188I TCNGA 3 cut(s) 205, 758, 1175
Hpy188III TCNNGA 6 cut(s) 461, 609, 751, 786, 1073, 1188
Hpy8I GTNNAC 3 cut(s) 199, 377, 695
HpyAV CCTTC 4 cut(s) 323, 337, 425, 693
HpyCH4III ACNGT 2 cut(s) 527, 589
HpyCH4IV ACGT 4 cut(s) 14, 100, 379, 708
HpyCH4V TGCA 5 cut(s) 4, 59, 442, 663, 818
HpyF10VI GCNNNNNNNGC 4 cut(s) 296, 298, 415, 843
HpyF3I CTNAG 4 cut(s) 270, 641, 1007, 1166
HpySE526I ACGT 4 cut(s) 14, 100, 379, 708
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 107, 164, 182, 440
HspAI GCGC 5 cut(s) 252, 288, 290, 299, 305
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 418
KroNI GCCGGC 1 cut(s) 420
Kzo9I GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
LmnI GCTCC 3 cut(s) 406, 427, 1175
Lsp1109I GCAGC 3 cut(s) 286, 367, 492
LweI GCATC 3 cut(s) 13, 286, 451
MaeI CTAG 6 cut(s) 461, 545, 609, 975, 1095, 1188
MaeII ACGT 4 cut(s) 14, 100, 379, 708
MaeIII GTNAC 2 cut(s) 106, 410
MalI GATC 9 cut(s) 165, 189, 674, 755, 784, 855, 881, 1052, 1086
MboI GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
MboII GAAGA 7 cut(s) 413, 440, 443, 689, 708, 1200, 1203
MflI RGATCY 1 cut(s) 1050
MhlI GDGCHC 2 cut(s) 1080, 1180
MlsI TGGCCA 1 cut(s) 135
MluCI AATT 8 cut(s) 20, 29, 54, 532, 631, 1041, 1066, 1183
MluNI TGGCCA 1 cut(s) 135
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 4 cut(s) 194, 405, 1111, 1164
MmeI TCCRAC 1 cut(s) 877
Mox20I TGGCCA 1 cut(s) 135
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 418
MscI TGGCCA 1 cut(s) 135
MseI TTAA 3 cut(s) 153, 531, 873
MslI CAYNNNNRTG 2 cut(s) 177, 770
Msp20I TGGCCA 1 cut(s) 135
MspA1I CMGCKG 2 cut(s) 277, 483
MspI CCGG 2 cut(s) 249, 419
MvnI CGCG 4 cut(s) 195, 288, 290, 305
MwoI GCNNNNNNNGC 4 cut(s) 296, 298, 415, 843
NaeI GCCGGC 1 cut(s) 420
NarI GGCGCC 1 cut(s) 252
NdeII GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
NgoMIV GCCGGC 1 cut(s) 418
NheI GCTAGC 1 cut(s) 1094
NlaIII CATG 4 cut(s) 107, 164, 182, 440
NlaIV GGNNCC 3 cut(s) 253, 423, 1197
NmeAIII GCCGAG 2 cut(s) 260, 283
NsbI TGCGCA 1 cut(s) 300
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 67
PaeR7I CTCGAG 1 cut(s) 314
PauI GCGCGC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 420
PkrI GCNGC 6 cut(s) 194, 256, 276, 287, 357, 482
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 4 cut(s) 194, 404, 1111, 1164
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 380
PmlI CACGTG 1 cut(s) 380
PpsI GAGTC 4 cut(s) 194, 404, 1111, 1164
Ppu21I YACGTR 1 cut(s) 380
PpuMI RGGWCCY 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 1080
Psp5II RGGWCCY 1 cut(s) 310
PspCI CACGTG 1 cut(s) 380
PspN4I GGNNCC 3 cut(s) 253, 423, 1197
PspPI GGNCC 2 cut(s) 310, 702
PspPPI RGGWCCY 1 cut(s) 310
PspXI VCTCGAGB 1 cut(s) 314
PsuI RGATCY 1 cut(s) 1050
PteI GCGCGC 1 cut(s) 288
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 5 cut(s) 183, 321, 543, 564, 667
RsaNI GTAC 5 cut(s) 182, 320, 542, 563, 666
RseI CAYNNNNRTG 2 cut(s) 177, 770
SacI GAGCTC 1 cut(s) 1080
SaqAI TTAA 3 cut(s) 153, 531, 873
SatI GCNGC 6 cut(s) 193, 255, 275, 286, 356, 481
Sau3AI GATC 9 cut(s) 163, 187, 672, 753, 782, 853, 879, 1050, 1084
Sau96I GGNCC 2 cut(s) 310, 702
SchI GAGTC 4 cut(s) 194, 405, 1111, 1164
SduI GDGCHC 2 cut(s) 1080, 1180
SfaNI GCATC 3 cut(s) 13, 286, 451
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 67
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 2 cut(s) 177, 770
SmlI CTYRAG 2 cut(s) 314, 1079
SmoI CTYRAG 2 cut(s) 314, 1079
SpeI ACTAGT 1 cut(s) 974
Sse9I AATT 8 cut(s) 20, 29, 54, 532, 631, 1041, 1066, 1183
SsiI CCGC 8 cut(s) 147, 193, 220, 232, 255, 277, 286, 483
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 6 cut(s) 461, 545, 609, 975, 1095, 1188
SstI GAGCTC 1 cut(s) 1080
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 527, 589
TaiI ACGT 4 cut(s) 17, 103, 382, 711
TaqI TCGA 7 cut(s) 67, 315, 399, 454, 675, 1003, 1039
TasI AATT 8 cut(s) 20, 29, 54, 532, 631, 1041, 1066, 1183
TatI WGTACW 1 cut(s) 665
TauI GCSGC 3 cut(s) 195, 257, 288
Tru1I TTAA 3 cut(s) 153, 531, 873
Tru9I TTAA 3 cut(s) 153, 531, 873
TscAI CASTG 1 cut(s) 274
TseI GCWGC 3 cut(s) 274, 355, 480
TspDTI ATGAA 2 cut(s) 22, 927
TspGWI ACGGA 2 cut(s) 98, 350
TspRI CASTG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 4 cut(s) 20, 29, 1041, 1183
XbaI TCTAGA 3 cut(s) 460, 608, 1187
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 6 cut(s) 461, 545, 609, 975, 1095, 1188
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.