pycom08g04520

positive regulation of Notch signaling pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Reverse (-)
3433913 .. 3437913
4001 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
ATGTTGTCTTGTGTTGTAGGGGTTAGAAATCTAAATCTTCCACTTTTAACCGATTTTGCGTCTTCTCGATCATACGGCCAGTGCATGGCAGTCGATCGGTGCAAAAATGGGTTAACCTCCGTGTTAGAAAAGCTCGAATTTGATCATGTTAAAGTACAAATTTGTTCATGTTTGCAGGCAGTGGAAGAAGTGGGAGTGGCAATGAGCGGAATGTACAAAACAATCAAACTACCTCAGGTGTGGAAACCTTCCCTTTACATGTACCTCTCCTTGGCCCTCAGTATTAACACTCAGGAAGGTAATTTTTATTGGTACACTGACCCTTCAGCTGGCCCTGCATTCTCTCAGGAGTTTGTTGGGATGATTTATGCAGTTGGTGCAGTGGCTTCGATAGTAGGAGTTCTGATCTACCACAAGACTCTGAAAAACTACCCTTTTCGGAACCTGGTCTTCTTTGCTCAACTTCTCTACGCCATGTCCGGACTTCTCGACCTAACTTTCATCTTAAGGTGGAACCTAGCTCTAGGCATTCCCGACTACTTCTTTGTGATCATGGAAGAATGCGTGTTTCGAATCGTTACCAAGATCCGATGGATGCCTATGATCGTACTGAGCACGAGGCTCTGCCCTCTAGGCATCGAGGGCACGTTCTTCGCGCTGTTGATGTGTATCGACAGCCTTGGCTCGCTGTCGTCGAAATGGGGTGGAGGAGCAGTGCTTCATGCCTTGAGTTTAGGTGCCCAATTTTTTAGGACCACCGCAACCGAGATCGTAATTGTCTATTTTACCCTCAACTCCGACGCGTCCCTAACCTACAGACCCTGGTCAAAGTCTTCTTCAACTCTCTCCCACTCCCTTTCTTCCTCCGGCTGTCGCGTCTTCTCTACGAACCCTAATTTTGCTGCAAACTTTTGGGTCGTCACTGTCAGCTCTCACATGGCGAACAAGCACACGATCATTCTAATGCAAACTTCTCACAACAAAGCAAGTAGAACATTTATGGACTATGATTCTATAAGTCAAGCAATGGATGGTATATGTGGACTATATGAGAGGAAGCTGAGGGAGTTAAATCCAGCAATTAGAGATATCTCTTACGACATCGGAGATCTCTACAATTTCATTGATGGTCTTGCTGACATGAGCGCTTTAGTTTATGACCACTCAATTCAGGGATATCTCCCATACGACAGACAGTGGATTAAACAACGGACGCTTCGACATCTGCAGAAACTTGCACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ER Lectin_C

Protein Analysis

415

Amino Acids

46.56

Weight (kDa)

8.43

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 55 - 259 1.1e-52 BT1 family
ER PF01133 316 - 412 5.9e-39 Enhancer of rudimentary
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 85
AccBSI CCGCTC 1 cut(s) 207
AccII CGCG 3 cut(s) 656, 803, 876
AccIII TCCGGA 1 cut(s) 479
AciI CCGC 2 cut(s) 207, 759
AclWI GGATC 1 cut(s) 580
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 2 cut(s) 137, 159
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 5 cut(s) 156, 215, 263, 314, 609
AfeI AGCGCT 1 cut(s) 1147
AfiI CCNNNNNNNGG 3 cut(s) 85, 271, 329
AflII CTTAAG 1 cut(s) 505
AflIII ACRYGT 2 cut(s) 258, 801
AgsI TTSAA 1 cut(s) 840
AjnI CCWGG 2 cut(s) 444, 821
AluBI AGCT 5 cut(s) 133, 329, 521, 930, 1060
AluI AGCT 5 cut(s) 133, 329, 521, 930, 1060
Alw21I GWGCWC 1 cut(s) 617
AlwI GGATC 1 cut(s) 580
AlwNI CAGNNNCTG 1 cut(s) 822
Aor13HI TCCGGA 1 cut(s) 479
Aor51HI AGCGCT 1 cut(s) 1147
AoxI GGCC 3 cut(s) 76, 273, 331
ApeKI GCWGC 1 cut(s) 902
ApoI RAATTY 2 cut(s) 137, 159
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AspLEI GCGC 2 cut(s) 658, 1148
AspS9I GGNCC 3 cut(s) 274, 332, 753
AsuII TTCGAA 1 cut(s) 571
AvaII GGWCC 1 cut(s) 753
AxyI CCTNAGG 1 cut(s) 234
BaeGI GKGCMC 2 cut(s) 647, 742
BanI GGYRCC 1 cut(s) 737
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 4 cut(s) 54, 442, 825, 871
Bbv12I GWGCWC 1 cut(s) 617
BbvCI CCTCAGC 1 cut(s) 1061
BbvI GCAGC 1 cut(s) 889
BccI CCATC 3 cut(s) 585, 1025, 1121
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 6 cut(s) 73, 107, 369, 403, 634, 668
BciT130I CCWGG 2 cut(s) 446, 823
BclI TGATCA 2 cut(s) 142, 549
BfaI CTAG 3 cut(s) 518, 524, 632
BfmI CTRYAG 2 cut(s) 814, 1226
BfoI RGCGCY 1 cut(s) 1149
BfrI CTTAAG 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 633
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 1 cut(s) 903
BlsI GCNGC 1 cut(s) 904
Bme1390I CCNGG 2 cut(s) 446, 823
Bme18I GGWCC 1 cut(s) 753
BmgT120I GGNCC 3 cut(s) 274, 332, 753
BmiI GGNNCC 3 cut(s) 443, 515, 739
BmrFI CCNGG 2 cut(s) 446, 823
BmsI GCATC 2 cut(s) 585, 645
BoxI GACNNNNGTC 1 cut(s) 823
BpiI GAAGAC 4 cut(s) 54, 442, 825, 871
Bpu10I CCTNAGC 1 cut(s) 1061
Bpu14I TTCGAA 1 cut(s) 571
BpuEI CTTGAG 1 cut(s) 748
BsaBI GATNNNNATC 1 cut(s) 668
BsaJI CCNNGG 3 cut(s) 270, 679, 821
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 271, 329
Bse1I ACTGG 1 cut(s) 79
Bse21I CCTNAGG 1 cut(s) 234
Bse3DI GCAATG 2 cut(s) 207, 1032
Bse8I GATNNNNATC 1 cut(s) 668
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 2 cut(s) 446, 823
BseDI CCNNGG 3 cut(s) 270, 679, 821
BseGI GGATG 3 cut(s) 366, 600, 1036
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 3 cut(s) 85, 271, 329
BseMI GCAATG 2 cut(s) 207, 1032
BseMII CTCAG 6 cut(s) 248, 292, 305, 359, 602, 1052
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 723
BseSI GKGCMC 2 cut(s) 647, 742
BseXI GCAGC 1 cut(s) 889
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 3 cut(s) 656, 803, 876
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 3 cut(s) 78, 275, 333
BshNI GGYRCC 1 cut(s) 737
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 617
BsiSI CCGG 2 cut(s) 480, 867
BslFI GGGAC 1 cut(s) 790
BslI CCNNNNNNNGG 3 cut(s) 85, 271, 329
BsmFI GGGAC 1 cut(s) 790
BsmI GAATGC 3 cut(s) 338, 528, 566
BsnI GGCC 3 cut(s) 78, 275, 333
Bsp119I TTCGAA 1 cut(s) 571
Bsp1286I GDGCHC 3 cut(s) 617, 647, 742
Bsp13I TCCGGA 1 cut(s) 479
Bsp1407I TGTACA 1 cut(s) 213
BspACI CCGC 2 cut(s) 207, 759
BspANI GGCC 3 cut(s) 78, 275, 333
BspCNI CTCAG 6 cut(s) 247, 291, 304, 358, 603, 1053
BspEI TCCGGA 1 cut(s) 479
BspFNI CGCG 3 cut(s) 656, 803, 876
BspLI GGNNCC 3 cut(s) 443, 515, 739
BspMAI CTGCAG 1 cut(s) 1230
BspPI GGATC 1 cut(s) 580
BspT104I TTCGAA 1 cut(s) 571
BspT107I GGYRCC 1 cut(s) 737
BspTI CTTAAG 1 cut(s) 505
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 207, 1032
BsrGI TGTACA 1 cut(s) 213
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 3 cut(s) 270, 679, 821
BssSI CACGAG 1 cut(s) 616
BssT1I CCWWGG 2 cut(s) 270, 679
Bst2BI CACGAG 1 cut(s) 616
Bst2UI CCWGG 2 cut(s) 446, 823
Bst4CI ACNGT 2 cut(s) 925, 1197
BstAFI CTTAAG 1 cut(s) 505
BstAPI GCANNNNNTGC 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 213
BstBI TTCGAA 1 cut(s) 571
BstC8I GCNNGC 3 cut(s) 177, 331, 686
BstDEI CTNAG 6 cut(s) 234, 278, 291, 345, 611, 1061
BstF5I GGATG 3 cut(s) 366, 600, 1036
BstFNI CGCG 3 cut(s) 656, 803, 876
BstH2I RGCGCY 1 cut(s) 1149
BstHHI GCGC 2 cut(s) 658, 1148
BstMCI CGRYCG 1 cut(s) 97
BstMWI GCNNNNNNNGC 4 cut(s) 335, 377, 633, 642
BstNI CCWGG 2 cut(s) 446, 823
BstNSI RCATGY 1 cut(s) 262
BstPAI GACNNNNGTC 1 cut(s) 823
BstSCI CCNGG 2 cut(s) 444, 821
BstSFI CTRYAG 2 cut(s) 814, 1226
BstSLI GKGCMC 2 cut(s) 647, 742
BstUI CGCG 3 cut(s) 656, 803, 876
BstV1I GCAGC 1 cut(s) 889
BstV2I GAAGAC 4 cut(s) 54, 442, 825, 871
BstX2I RGATCY 2 cut(s) 585, 1108
BstYI RGATCY 2 cut(s) 585, 1108
Bsu36I CCTNAGG 1 cut(s) 234
BsuRI GGCC 3 cut(s) 78, 275, 333
BtsCI GGATG 3 cut(s) 366, 600, 1036
BtsI GCAGTG 3 cut(s) 186, 387, 720
BtsIMutI CAGTG 7 cut(s) 86, 186, 315, 387, 720, 921, 1202
Cac8I GCNNGC 3 cut(s) 177, 331, 686
CaiI CAGNNNCTG 1 cut(s) 822
CfoI GCGC 2 cut(s) 658, 1148
Cfr13I GGNCC 3 cut(s) 274, 332, 753
CseI GACGC 5 cut(s) 48, 792, 809, 865, 1222
CsiI ACCWGGT 1 cut(s) 444
Csp6I GTAC 5 cut(s) 155, 214, 262, 313, 608
CviAII CATG 9 cut(s) 85, 146, 168, 259, 475, 553, 722, 937, 1141
CviQI GTAC 5 cut(s) 155, 214, 262, 313, 608
DdeI CTNAG 6 cut(s) 234, 278, 291, 345, 611, 1061
EaeI YGGCCR 1 cut(s) 76
Eco130I CCWWGG 2 cut(s) 270, 679
Eco32I GATATC 2 cut(s) 1090, 1178
Eco47I GGWCC 1 cut(s) 753
Eco47III AGCGCT 1 cut(s) 1147
Eco57I CTGAAG 1 cut(s) 309
Eco81I CCTNAGG 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 444, 821
EcoRV GATATC 2 cut(s) 1090, 1178
EcoT14I CCWWGG 2 cut(s) 270, 679
ErhI CCWWGG 2 cut(s) 270, 679
FaeI CATG 9 cut(s) 88, 149, 171, 262, 478, 556, 725, 940, 1144
FaqI GGGAC 1 cut(s) 790
FatI CATG 9 cut(s) 84, 145, 167, 258, 474, 552, 721, 936, 1140
FbaI TGATCA 2 cut(s) 142, 549
Fnu4HI GCNGC 1 cut(s) 903
FokI GGATG 3 cut(s) 373, 607, 1043
Fsp4HI GCNGC 1 cut(s) 903
FspBI CTAG 3 cut(s) 518, 524, 632
GlaI GCGC 2 cut(s) 657, 1147
GluI GCNGC 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 1149
HaeIII GGCC 3 cut(s) 78, 275, 333
HapII CCGG 2 cut(s) 480, 867
HgaI GACGC 5 cut(s) 48, 792, 809, 865, 1222
HhaI GCGC 2 cut(s) 658, 1148
Hin1II CATG 9 cut(s) 88, 149, 171, 262, 478, 556, 725, 940, 1144
Hin6I GCGC 2 cut(s) 656, 1146
HinP1I GCGC 2 cut(s) 656, 1146
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 3 cut(s) 418, 573, 1010
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 2 cut(s) 480, 867
Hpy166II GTNNAC 3 cut(s) 114, 315, 1043
Hpy188I TCNGA 6 cut(s) 405, 423, 441, 590, 799, 1106
Hpy188III TCNNGA 6 cut(s) 66, 293, 347, 480, 488, 533
Hpy8I GTNNAC 3 cut(s) 114, 315, 1043
Hpy99I CGWCG 2 cut(s) 697, 803
HpyAV CCTTC 3 cut(s) 258, 290, 333
HpyCH4III ACNGT 2 cut(s) 925, 1197
HpyCH4IV ACGT 1 cut(s) 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 335, 377, 633, 642
HpyF3I CTNAG 6 cut(s) 234, 278, 291, 345, 611, 1061
HpySE526I ACGT 1 cut(s) 647
Hsp92II CATG 9 cut(s) 88, 149, 171, 262, 478, 556, 725, 940, 1144
HspAI GCGC 2 cut(s) 656, 1146
Kpn2I TCCGGA 1 cut(s) 479
Ksp22I TGATCA 2 cut(s) 142, 549
KspAI GTTAAC 1 cut(s) 114
LmnI GCTCC 1 cut(s) 710
Lsp1109I GCAGC 1 cut(s) 889
LweI GCATC 2 cut(s) 585, 645
MabI ACCWGGT 1 cut(s) 444
MaeI CTAG 3 cut(s) 518, 524, 632
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 2 cut(s) 577, 919
MbiI CCGCTC 1 cut(s) 207
MflI RGATCY 2 cut(s) 585, 1108
MhlI GDGCHC 3 cut(s) 617, 647, 742
MluCI AATT 9 cut(s) 137, 159, 301, 743, 774, 895, 1080, 1117, 1167
MluI ACGCGT 1 cut(s) 801
MlyI GAGTC 1 cut(s) 412
MmeI TCCRAC 1 cut(s) 822
MroI TCCGGA 1 cut(s) 479
MseI TTAA 7 cut(s) 47, 113, 150, 285, 506, 1070, 1203
MslI CAYNNNNRTG 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 505
MspI CCGG 2 cut(s) 480, 867
MspR9I CCNGG 2 cut(s) 446, 823
Mva1269I GAATGC 3 cut(s) 338, 528, 566
MvaI CCWGG 2 cut(s) 446, 823
MvnI CGCG 3 cut(s) 656, 803, 876
MwoI GCNNNNNNNGC 4 cut(s) 335, 377, 633, 642
NlaIII CATG 9 cut(s) 88, 149, 171, 262, 478, 556, 725, 940, 1144
NlaIV GGNNCC 3 cut(s) 443, 515, 739
NmuCI GTSAC 1 cut(s) 919
NspI RCATGY 1 cut(s) 262
NspV TTCGAA 1 cut(s) 571
PciI ACATGT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 692, 1216
PctI GAATGC 3 cut(s) 338, 528, 566
PfeI GAWTC 2 cut(s) 573, 1010
PflMI CCANNNNNTGG 1 cut(s) 85
PkrI GCNGC 1 cut(s) 904
Ple19I CGATCG 1 cut(s) 97
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
PscI ACATGT 1 cut(s) 258
PshAI GACNNNNGTC 1 cut(s) 823
Psp6I CCWGG 2 cut(s) 444, 821
PspGI CCWGG 2 cut(s) 444, 821
PspN4I GGNNCC 3 cut(s) 443, 515, 739
PspPI GGNCC 3 cut(s) 274, 332, 753
PstI CTGCAG 1 cut(s) 1230
PstNI CAGNNNCTG 1 cut(s) 822
PsuI RGATCY 2 cut(s) 585, 1108
PvuI CGATCG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 5 cut(s) 156, 215, 263, 314, 609
RsaNI GTAC 5 cut(s) 155, 214, 262, 313, 608
RseI CAYNNNNRTG 1 cut(s) 962
SaqAI TTAA 7 cut(s) 47, 113, 150, 285, 506, 1070, 1203
SatI GCNGC 1 cut(s) 903
Sau96I GGNCC 3 cut(s) 274, 332, 753
SchI GAGTC 1 cut(s) 412
ScrFI CCNGG 2 cut(s) 446, 823
SduI GDGCHC 3 cut(s) 617, 647, 742
SexAI ACCWGGT 1 cut(s) 444
SfaNI GCATC 2 cut(s) 585, 645
SfcI CTRYAG 2 cut(s) 814, 1226
SfuI TTCGAA 1 cut(s) 571
SinI GGWCC 1 cut(s) 753
SmiMI CAYNNNNRTG 1 cut(s) 962
SmlI CTYRAG 2 cut(s) 505, 727
SmoI CTYRAG 2 cut(s) 505, 727
Sse9I AATT 9 cut(s) 137, 159, 301, 743, 774, 895, 1080, 1117, 1167
SsiI CCGC 2 cut(s) 207, 759
SspMI CTAG 3 cut(s) 518, 524, 632
StyD4I CCNGG 2 cut(s) 444, 821
StyI CCWWGG 2 cut(s) 270, 679
TaaI ACNGT 2 cut(s) 925, 1197
TaiI ACGT 1 cut(s) 650
TasI AATT 9 cut(s) 137, 159, 301, 743, 774, 895, 1080, 1117, 1167
TatI WGTACW 2 cut(s) 154, 213
TfiI GAWTC 2 cut(s) 573, 1010
Tru1I TTAA 7 cut(s) 47, 113, 150, 285, 506, 1070, 1203
Tru9I TTAA 7 cut(s) 47, 113, 150, 285, 506, 1070, 1203
TscAI CASTG 7 cut(s) 86, 186, 322, 387, 720, 928, 1202
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 1 cut(s) 902
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 4 cut(s) 156, 490, 710, 1111
TspGWI ACGGA 2 cut(s) 109, 1225
TspRI CASTG 7 cut(s) 86, 186, 322, 387, 720, 928, 1202
Van91I CCANNNNNTGG 1 cut(s) 85
Vha464I CTTAAG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 753
XapI RAATTY 2 cut(s) 137, 159
XceI RCATGY 1 cut(s) 262
XspI CTAG 3 cut(s) 518, 524, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.