pycom08g07880

acetyltransferase At3g50280-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Forward (+)
6362329 .. 6363083
755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGAGAAAACTACACCCAATATTTTCACAACCACCTGCCCTCGAGCGCTGGTTTCCGGACGTTTATGGCCCGATTTTTAACCTGCCTTGTGCAGATATGAAGATCATGGCCAGACTCGAGGGTCCGCAACTAAGAGAGAGTTTTCCACTTCTCTTCGGAATTCGTAGCAAACTCAAAGCCAAGGCAAATGCAAATTATTCTTCTAGTCCCAACACAATCTCCTCGTTCCAGTCCTTGCCTGCACTGGTCTGGAGGTGCATTATACAAGCATGTAATGCAACTCCAGGCCAAGTAACCACTTGTGTGGTGGGTTCCAATAAGAGACCAAAACCAGAGCTACCCTTGTCCCAACATTATTTTGGAAAATTTGTCAGTGCCTCAAGGGGGTTATTGCATGAGGCAGTGGTGAACCACAATGATACGGCGATACTACGACGTATAGATACATGGCTGCAGTCTCCTACCGCCTACCAATTTGGACGGCATCTGGATCCGTATCTTGTGACCATACGCAGTTCTCCGAGTTACGAAATGTATGGAAATGAATCCGGAATGGGGAAACAATTGGCGACGAGATACGGGTGGGTGGATAAGTATGACGGGAGAGTAGTATCGTATCCTGGTCGCCGAGGAGGCGGAAGCATCAACCTAAAAGCTTGTCTTTCGCCAGAATCGATGAAAGCTCTTGAATGTGCAAACTAA

Protein Analysis

234

Amino Acids

26.0

Weight (kDa)

9.59

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 57 - 230 4.5e-13 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
AasI GACNNNNNNGTC 1 cut(s) 120
Acc36I ACCTGC 2 cut(s) 43, 90
AccIII TCCGGA 2 cut(s) 55, 548
AciI CCGC 3 cut(s) 125, 465, 636
AclWI GGATC 2 cut(s) 485, 498
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 2 cut(s) 159, 365
AfeI AGCGCT 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 384, 555
AgsI TTSAA 1 cut(s) 689
AjnI CCWGG 2 cut(s) 283, 619
AleI CACNNNNGTG 1 cut(s) 302
AluBI AGCT 3 cut(s) 337, 656, 683
AluI AGCT 3 cut(s) 337, 656, 683
Alw26I GTCTC 2 cut(s) 316, 462
AlwI GGATC 2 cut(s) 485, 498
Ama87I CYCGRG 2 cut(s) 41, 116
Aor13HI TCCGGA 2 cut(s) 55, 548
Aor51HI AGCGCT 1 cut(s) 47
AoxI GGCC 3 cut(s) 67, 108, 286
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 2 cut(s) 159, 365
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 68, 122
AsuHPI GGTGA 1 cut(s) 418
AvaI CYCGRG 2 cut(s) 41, 116
AvaII GGWCC 1 cut(s) 122
BalI TGGCCA 1 cut(s) 110
BamHI GGATCC 1 cut(s) 490
BbvI GCAGC 1 cut(s) 438
BceAI ACGGC 2 cut(s) 438, 497
BciT130I CCWGG 2 cut(s) 285, 621
BciVI GTATCC 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 316, 462
BfaI CTAG 1 cut(s) 204
BfmI CTRYAG 1 cut(s) 452
BfoI RGCGCY 1 cut(s) 49
BfuAI ACCTGC 2 cut(s) 43, 90
BfuI GTATCC 1 cut(s) 627
BglI GCCNNNNNGGC 1 cut(s) 633
BisI GCNGC 1 cut(s) 452
BlsI GCNGC 1 cut(s) 453
Bme1390I CCNGG 2 cut(s) 285, 621
Bme18I GGWCC 1 cut(s) 122
BmeT110I CYCGRG 2 cut(s) 41, 116
BmgT120I GGNCC 2 cut(s) 68, 122
BmiI GGNNCC 3 cut(s) 123, 313, 492
BmrFI CCNGG 2 cut(s) 285, 621
BmsI GCATC 2 cut(s) 493, 651
BpmI CTGGAG 2 cut(s) 267, 271
BpuEI CTTGAG 1 cut(s) 364
Bsa29I ATCGAT 1 cut(s) 674
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 2 cut(s) 180, 628
BsaWI WCCGGW 2 cut(s) 55, 548
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 555
Bse1I ACTGG 2 cut(s) 229, 249
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 2 cut(s) 55, 548
BseBI CCWGG 2 cut(s) 285, 621
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 2 cut(s) 180, 628
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 384, 555
BseNI ACTGG 2 cut(s) 229, 249
BseRI GAGGAG 2 cut(s) 211, 645
BseXI GCAGC 1 cut(s) 438
BsgI GTGCAG 2 cut(s) 111, 225
BshFI GGCC 3 cut(s) 69, 110, 288
BshVI ATCGAT 1 cut(s) 674
BsiHKCI CYCGRG 2 cut(s) 41, 116
BsiSI CCGG 2 cut(s) 56, 549
BslFI GGGAC 2 cut(s) 192, 331
BslI CCNNNNNNNGG 2 cut(s) 384, 555
BsmAI GTCTC 2 cut(s) 316, 462
BsmFI GGGAC 2 cut(s) 192, 331
BsnI GGCC 3 cut(s) 69, 110, 288
Bso31I GGTCTC 1 cut(s) 316
BsoBI CYCGRG 2 cut(s) 41, 116
Bsp13I TCCGGA 2 cut(s) 55, 548
Bsp143I GATC 2 cut(s) 102, 490
BspACI CCGC 3 cut(s) 125, 465, 636
BspANI GGCC 3 cut(s) 69, 110, 288
BspDI ATCGAT 1 cut(s) 674
BspEI TCCGGA 2 cut(s) 55, 548
BspLI GGNNCC 3 cut(s) 123, 313, 492
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 2 cut(s) 43, 90
BspPI GGATC 2 cut(s) 485, 498
BspTNI GGTCTC 1 cut(s) 316
BsrI ACTGG 2 cut(s) 229, 249
BssECI CCNNGG 2 cut(s) 180, 628
BssMI GATC 2 cut(s) 102, 490
BssT1I CCWWGG 1 cut(s) 180
Bst2UI CCWGG 2 cut(s) 285, 621
Bst6I CTCTTC 1 cut(s) 158
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 105, 493
BstMAI GTCTC 2 cut(s) 316, 462
BstMBI GATC 2 cut(s) 102, 490
BstMWI GCNNNNNNNGC 2 cut(s) 275, 633
BstNI CCWGG 2 cut(s) 285, 621
BstNSI RCATGY 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 283, 619
BstSFI CTRYAG 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 490
BstXI CCANNNNNNTGG 1 cut(s) 304
BstYI RGATCY 1 cut(s) 490
Bsu15I ATCGAT 1 cut(s) 674
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 3 cut(s) 69, 110, 288
BsuTUI ATCGAT 1 cut(s) 674
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 3 cut(s) 242, 379, 408
BveI ACCTGC 2 cut(s) 43, 90
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 68, 122
ClaI ATCGAT 1 cut(s) 674
CviAII CATG 4 cut(s) 106, 270, 395, 447
CviJI RGCY 8 cut(s) 69, 110, 179, 288, 337, 451, 656, 683
CviKI_1 RGCY 8 cut(s) 69, 110, 179, 288, 337, 451, 656, 683
DdeI CTNAG 1 cut(s) 131
DpnI GATC 2 cut(s) 104, 492
DpnII GATC 2 cut(s) 102, 490
DrdI GACNNNNNNGTC 1 cut(s) 120
DseDI GACNNNNNNGTC 1 cut(s) 120
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 122
Eco47III AGCGCT 1 cut(s) 47
Eco88I CYCGRG 2 cut(s) 41, 116
EcoRI GAATTC 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 283, 619
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 4 cut(s) 109, 273, 398, 450
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 2 cut(s) 192, 331
FatI CATG 4 cut(s) 105, 269, 394, 446
Fnu4HI GCNGC 1 cut(s) 452
Fsp4HI GCNGC 1 cut(s) 452
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 47
GluI GCNGC 1 cut(s) 452
GsuI CTGGAG 2 cut(s) 267, 271
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 3 cut(s) 69, 110, 288
HapII CCGG 2 cut(s) 56, 549
HhaI GCGC 1 cut(s) 48
Hin1II CATG 4 cut(s) 109, 273, 398, 450
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 654
HinfI GANTC 3 cut(s) 114, 545, 671
HpaII CCGG 2 cut(s) 56, 549
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 2 cut(s) 158, 522
Hpy188III TCNNGA 5 cut(s) 56, 250, 488, 549, 686
Hpy8I GTNNAC 1 cut(s) 409
Hpy99I CGWCG 2 cut(s) 438, 574
HpyCH4IV ACGT 2 cut(s) 60, 436
HpyCH4V TGCA 8 cut(s) 92, 191, 242, 258, 278, 394, 454, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 633
HpyF3I CTNAG 1 cut(s) 131
HpySE526I ACGT 2 cut(s) 60, 436
Hsp92II CATG 4 cut(s) 109, 273, 398, 450
HspAI GCGC 1 cut(s) 46
Kpn2I TCCGGA 2 cut(s) 55, 548
Kzo9I GATC 2 cut(s) 102, 490
Lsp1109I GCAGC 1 cut(s) 438
LweI GCATC 2 cut(s) 493, 651
MaeI CTAG 1 cut(s) 204
MaeII ACGT 2 cut(s) 60, 436
MaeIII GTNAC 3 cut(s) 292, 502, 524
MalI GATC 2 cut(s) 104, 492
MboI GATC 2 cut(s) 102, 490
MboII GAAGA 3 cut(s) 112, 145, 192
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 1 cut(s) 490
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 5 cut(s) 159, 193, 365, 473, 563
MluNI TGGCCA 1 cut(s) 110
MlyI GAGTC 1 cut(s) 108
MnlI CCTC 8 cut(s) 50, 112, 232, 246, 388, 391, 623, 626
Mox20I TGGCCA 1 cut(s) 110
MroI TCCGGA 2 cut(s) 55, 548
MscI TGGCCA 1 cut(s) 110
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 302
Msp20I TGGCCA 1 cut(s) 110
MspI CCGG 2 cut(s) 56, 549
MspR9I CCNGG 2 cut(s) 285, 621
MunI CAATTG 1 cut(s) 563
MvaI CCWGG 2 cut(s) 285, 621
MwoI GCNNNNNNNGC 2 cut(s) 275, 633
NdeII GATC 2 cut(s) 102, 490
NlaIII CATG 4 cut(s) 109, 273, 398, 450
NlaIV GGNNCC 3 cut(s) 123, 313, 492
NmeAIII GCCGAG 1 cut(s) 653
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 273
OliI CACNNNNGTG 1 cut(s) 302
PaeR7I CTCGAG 2 cut(s) 41, 116
PaqCI CACCTGC 1 cut(s) 43
PcsI WCGNNNNNNNCGW 1 cut(s) 671
PfeI GAWTC 2 cut(s) 545, 671
PkrI GCNGC 1 cut(s) 453
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
Psp6I CCWGG 2 cut(s) 283, 619
PspGI CCWGG 2 cut(s) 283, 619
PspN4I GGNNCC 3 cut(s) 123, 313, 492
PspPI GGNCC 2 cut(s) 68, 122
PspXI VCTCGAGB 2 cut(s) 41, 116
PstI CTGCAG 1 cut(s) 456
PsuI RGATCY 1 cut(s) 490
RseI CAYNNNNRTG 1 cut(s) 302
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 452
Sau3AI GATC 2 cut(s) 102, 490
Sau96I GGNCC 2 cut(s) 68, 122
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 2 cut(s) 285, 621
SetI ASST 9 cut(s) 37, 63, 84, 257, 339, 439, 651, 658, 685
SfaNI GCATC 2 cut(s) 493, 651
SfcI CTRYAG 1 cut(s) 452
Sfr274I CTCGAG 2 cut(s) 41, 116
SinI GGWCC 1 cut(s) 122
SlaI CTCGAG 2 cut(s) 41, 116
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 3 cut(s) 41, 116, 379
SmoI CTYRAG 3 cut(s) 41, 116, 379
Sse9I AATT 5 cut(s) 159, 193, 365, 473, 563
SsiI CCGC 3 cut(s) 125, 465, 636
SspI AATATT 1 cut(s) 21
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 283, 619
StyI CCWWGG 1 cut(s) 180
TaiI ACGT 2 cut(s) 63, 439
TaqI TCGA 3 cut(s) 42, 117, 674
TasI AATT 5 cut(s) 159, 193, 365, 473, 563
TfiI GAWTC 2 cut(s) 545, 671
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 3 cut(s) 249, 379, 408
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 3 cut(s) 113, 558, 692
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 3 cut(s) 249, 379, 408
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 2 cut(s) 159, 365
XceI RCATGY 1 cut(s) 273
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 356
XhoI CTCGAG 2 cut(s) 41, 116
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.