pycom08g09140

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Reverse (-)
7370477 .. 7372039
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGACATTCCGTCAGGTCATAGACAGATCTTTTGGCTTTGATACTGCGGTTGAGGAGGCTCAACGTGCCATTAATGCAGCTCATGTTGAAGCTGAAAGTATTGAGAACGGTATTGGTGTTGTGAAGCTAATGGGACGCTACAGTGGTTTCATAGCTATGTATGCTACACTTGCCAGCCGAGATGTGGACTGTTGTTTGATTCCAGAGTCTCCCTTCTATCTTGAAGGGAAAGGTGGACTTTTTGAGTACATTGAAAAAAGACTAAAAGAAAATGGGCATATGGTTATTGTGATAGCTGAGGGTGCAGGACAAGATCTACTGTCAGAGAGCTTGCAATCCATGAACCAGCAGGATGCCTCAGGAAACAAGCTACTTAAAGATGTTGGGCTATGGATTTCTCAGAGGATAAAGAGTCACTTCGGAAGACAACAGAAGCTTGCCATTAATCTTAAGTATATAGATCCAACATACATGATCAGGGCTATTCCAAGCAATGCAGCCGACAATGTCTACTGCACACTCCTTGCTCAAAGTGCTGTTCATGGAGCAATGGCAGGGTACACAGGCTTCACATGTGGGCCTGTCAATGGAAGGCATTCTTATATTCCTTTCAATCATATTACAGAGAAGCAGAACAAGGTTGTGATTACTGATAGGATGTGGGCGCGACTCCTTTCTTCAACCAACCAACCGAGTTTCTTGAACCCTAAAGACGTCAAGGAAGTCCTAGAAGAGGAAGAGCCTCCAACCCAAATGTTGGAAGGGGAAAACTGTAAAGATAGCAAGTTCGCAACAAAAGACATCCCTACCATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

273

Amino Acids

30.25

Weight (kDa)

5.74

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 7 - 181 5e-25 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 717
AccB7I CCANNNNNTGG 1 cut(s) 757
AccI GTMKAC 1 cut(s) 510
AccII CGCG 1 cut(s) 667
AciI CCGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 455
AcyI GRCGYC 1 cut(s) 714
AfaI GTAC 2 cut(s) 248, 560
AfiI CCNNNNNNNGG 4 cut(s) 184, 587, 733, 757
AflII CTTAAG 1 cut(s) 449
AflIII ACRYGT 1 cut(s) 572
AgsI TTSAA 6 cut(s) 89, 224, 254, 613, 681, 703
AhdI GACNNNNNGTC 1 cut(s) 9
AluBI AGCT 8 cut(s) 80, 92, 127, 155, 296, 330, 370, 436
AluI AGCT 8 cut(s) 80, 92, 127, 155, 296, 330, 370, 436
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 1 cut(s) 455
AoxI GGCC 1 cut(s) 578
ApeKI GCWGC 2 cut(s) 77, 497
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AseI ATTAAT 2 cut(s) 72, 444
Asp700I GAANNNNTTC 1 cut(s) 595
AspLEI GCGC 1 cut(s) 667
AspS9I GGNCC 1 cut(s) 578
AxyI CCTNAGG 1 cut(s) 358
BaeI ACNNNNGTAYC 2 cut(s) 33, 66
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 1 cut(s) 430
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 89, 509
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 213
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 1 cut(s) 139
BfrI CTTAAG 1 cut(s) 449
BglII AGATCT 2 cut(s) 26, 313
BisI GCNGC 2 cut(s) 78, 498
BlsI GCNGC 2 cut(s) 79, 499
BmeRI GACNNNNNGTC 1 cut(s) 9
BmgT120I GGNCC 1 cut(s) 578
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 714
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 587, 733, 757
Bse21I CCTNAGG 1 cut(s) 358
Bse3DI GCAATG 2 cut(s) 499, 555
BseGI GGATG 3 cut(s) 358, 663, 801
BseLI CCNNNNNNNGG 4 cut(s) 184, 587, 733, 757
BseMI GCAATG 2 cut(s) 499, 555
BseMII CTCAG 3 cut(s) 288, 372, 413
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 89, 509
BsgI GTGCAG 2 cut(s) 324, 499
Bsh1236I CGCG 1 cut(s) 667
BshFI GGCC 1 cut(s) 580
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 4 cut(s) 184, 587, 733, 757
BsmAI GTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 595
BsnI GGCC 1 cut(s) 580
Bsp143I GATC 4 cut(s) 26, 313, 460, 474
BspACI CCGC 1 cut(s) 47
BspANI GGCC 1 cut(s) 580
BspCNI CTCAG 3 cut(s) 289, 371, 412
BspFNI CGCG 1 cut(s) 667
BspHI TCATGA 1 cut(s) 815
BspPI GGATC 1 cut(s) 455
BspQI GCTCTTC 1 cut(s) 732
BspTI CTTAAG 1 cut(s) 449
BsrDI GCAATG 2 cut(s) 499, 555
BssMI GATC 4 cut(s) 26, 313, 460, 474
BssNI GRCGYC 1 cut(s) 714
Bst4CI ACNGT 5 cut(s) 110, 143, 191, 321, 773
Bst6I CTCTTC 2 cut(s) 726, 732
BstACI GRCGYC 1 cut(s) 714
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 175, 332, 438
BstDEI CTNAG 3 cut(s) 297, 358, 399
BstENI CCTNNNNNAGG 1 cut(s) 731
BstF5I GGATG 3 cut(s) 358, 663, 801
BstFNI CGCG 1 cut(s) 667
BstHHI GCGC 1 cut(s) 667
BstKTI GATC 4 cut(s) 29, 316, 463, 477
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 4 cut(s) 26, 313, 460, 474
BstMWI GCNNNNNNNGC 6 cut(s) 65, 74, 161, 170, 302, 533
BstNSI RCATGY 1 cut(s) 576
BstSFI CTRYAG 1 cut(s) 139
BstUI CGCG 1 cut(s) 667
BstV1I GCAGC 2 cut(s) 89, 509
BstV2I GAAGAC 1 cut(s) 430
BstX2I RGATCY 3 cut(s) 26, 313, 460
BstYI RGATCY 3 cut(s) 26, 313, 460
Bsu36I CCTNAGG 1 cut(s) 358
BsuRI GGCC 1 cut(s) 580
BtsCI GGATG 3 cut(s) 358, 663, 801
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 3 cut(s) 175, 332, 438
CciI TCATGA 1 cut(s) 815
CfoI GCGC 1 cut(s) 667
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 247, 559
CviAII CATG 7 cut(s) 83, 340, 472, 542, 573, 811, 816
CviQI GTAC 2 cut(s) 247, 559
DdeI CTNAG 3 cut(s) 297, 358, 399
DpnI GATC 4 cut(s) 28, 315, 462, 476
DpnII GATC 4 cut(s) 26, 313, 460, 474
DriI GACNNNNNGTC 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 726, 732
Eam1105I GACNNNNNGTC 1 cut(s) 9
EarI CTCTTC 2 cut(s) 726, 732
Eco81I CCTNAGG 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 731
FaeI CATG 7 cut(s) 86, 343, 475, 545, 576, 814, 819
FalI AAGNNNNNCTT 6 cut(s) 222, 254, 401, 433, 583, 615
FaqI GGGAC 1 cut(s) 147
FatI CATG 7 cut(s) 82, 339, 471, 541, 572, 810, 815
FauNDI CATATG 1 cut(s) 279
FbaI TGATCA 1 cut(s) 474
FblI GTMKAC 1 cut(s) 510
Fnu4HI GCNGC 2 cut(s) 78, 498
FokI GGATG 3 cut(s) 365, 670, 788
Fsp4HI GCNGC 2 cut(s) 78, 498
FspBI CTAG 1 cut(s) 728
GlaI GCGC 1 cut(s) 666
GluI GCNGC 2 cut(s) 78, 498
HaeIII GGCC 1 cut(s) 580
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 667
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 7 cut(s) 86, 343, 475, 545, 576, 814, 819
Hin6I GCGC 1 cut(s) 665
HinP1I GCGC 1 cut(s) 665
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 4 cut(s) 199, 206, 412, 669
Hpy166II GTNNAC 4 cut(s) 187, 236, 511, 561
Hpy188I TCNGA 3 cut(s) 325, 402, 422
Hpy188III TCNNGA 5 cut(s) 203, 221, 360, 700, 816
Hpy8I GTNNAC 4 cut(s) 187, 236, 511, 561
HpyAV CCTTC 4 cut(s) 218, 223, 585, 755
HpyCH4III ACNGT 5 cut(s) 110, 143, 191, 321, 773
HpyCH4IV ACGT 2 cut(s) 64, 714
HpyCH4V TGCA 5 cut(s) 77, 305, 334, 497, 516
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 74, 161, 170, 302, 533
HpyF3I CTNAG 3 cut(s) 297, 358, 399
HpySE526I ACGT 2 cut(s) 64, 714
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 7 cut(s) 86, 343, 475, 545, 576, 814, 819
HspAI GCGC 1 cut(s) 665
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 4 cut(s) 26, 313, 460, 474
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 2 cut(s) 89, 509
LweI GCATC 1 cut(s) 343
MaeI CTAG 1 cut(s) 728
MaeII ACGT 2 cut(s) 64, 714
MaeIII GTNAC 1 cut(s) 413
MalI GATC 4 cut(s) 28, 315, 462, 476
MboI GATC 4 cut(s) 26, 313, 460, 474
MboII GAAGA 4 cut(s) 435, 669, 743, 749
MflI RGATCY 3 cut(s) 26, 313, 460
MlyI GAGTC 3 cut(s) 215, 421, 663
MmeI TCCRAC 3 cut(s) 488, 738, 770
MnlI CCTC 7 cut(s) 46, 49, 292, 367, 396, 727, 753
MroXI GAANNNNTTC 1 cut(s) 595
MseI TTAA 4 cut(s) 72, 375, 444, 450
MslI CAYNNNNRTG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 449
Mva1269I GAATGC 1 cut(s) 595
MvnI CGCG 1 cut(s) 667
MwoI GCNNNNNNNGC 6 cut(s) 65, 74, 161, 170, 302, 533
NdeI CATATG 1 cut(s) 279
NdeII GATC 4 cut(s) 26, 313, 460, 474
NlaIII CATG 7 cut(s) 86, 343, 475, 545, 576, 814, 819
NmeAIII GCCGAG 1 cut(s) 203
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 576
PagI TCATGA 1 cut(s) 815
PciI ACATGT 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 732
PctI GAATGC 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 757
PkrI GCNGC 2 cut(s) 79, 499
PleI GAGTC 3 cut(s) 214, 420, 663
PpsI GAGTC 3 cut(s) 214, 420, 663
PscI ACATGT 1 cut(s) 572
PshBI ATTAAT 2 cut(s) 72, 444
PspPI GGNCC 1 cut(s) 578
PsuI RGATCY 3 cut(s) 26, 313, 460
PsyI GACNNNGTC 1 cut(s) 506
RsaI GTAC 2 cut(s) 248, 560
RsaNI GTAC 2 cut(s) 247, 559
RseI CAYNNNNRTG 1 cut(s) 155
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 4 cut(s) 72, 375, 444, 450
SatI GCNGC 2 cut(s) 78, 498
Sau3AI GATC 4 cut(s) 26, 313, 460, 474
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 3 cut(s) 215, 421, 663
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SsiI CCGC 1 cut(s) 47
SspMI CTAG 1 cut(s) 728
TaaI ACNGT 5 cut(s) 110, 143, 191, 321, 773
TaiI ACGT 2 cut(s) 67, 717
TatI WGTACW 1 cut(s) 246
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 4 cut(s) 72, 375, 444, 450
Tru9I TTAA 4 cut(s) 72, 375, 444, 450
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 2 cut(s) 77, 497
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 3 cut(s) 139, 356, 530
TspRI CASTG 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 757
Vha464I CTTAAG 1 cut(s) 449
VspI ATTAAT 2 cut(s) 72, 444
XagI CCTNNNNNAGG 1 cut(s) 731
XceI RCATGY 1 cut(s) 576
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 510
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 1 cut(s) 728
ZraI GACGTC 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.