pycom08g20110

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
N/A
Physical Location & Seq
Forward (+)
19989459 .. 19989891
433 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 357 bp
ATGCAGTCCACCGCTCCAGCTGCTACTAACGCTGCAAATGGTCCTTCTTCCTACTCAAATGGTGTCAATAATGGCGATTCATCTTCTTCTGAAAACGCTTGTACCCATCACCATGGAGACTTAGTTGGTGCTGGTGGCCCTGGTACTGGAGGATCTCTTGAAGAGGCTGGATTTTGGTATAAGAACTATGTGGCCTCGTTTCGTACTTGGAATCTCAAGCTTGGTAAAGTGCAGCAAAAAGTGTCAATACATAACCTGAAATGTCATAACAATCAATCCCTTTGTTTTGACTCGTTCATTATCTACGTGCTTATGTGCATGTATATACGGACCTTCTCTCAGCTTGAATGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0070013
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

12.78

Weight (kDa)

6.99

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 14
AciI CCGC 1 cut(s) 12
AclWI GGATC 1 cut(s) 160
AfaI GTAC 3 cut(s) 103, 145, 205
AfiI CCNNNNNNNGG 1 cut(s) 146
AgsI TTSAA 2 cut(s) 161, 347
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 3 cut(s) 20, 220, 343
AluI AGCT 3 cut(s) 20, 220, 343
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 1 cut(s) 160
AoxI GGCC 2 cut(s) 136, 192
ApeKI GCWGC 3 cut(s) 20, 32, 232
AspS9I GGNCC 3 cut(s) 41, 137, 330
AsuHPI GGTGA 1 cut(s) 101
AvaII GGWCC 2 cut(s) 41, 330
BaeI ACNNNNGTAYC 2 cut(s) 85, 118
BbvI GCAGC 3 cut(s) 7, 19, 244
BccI CCATC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 111
BisI GCNGC 3 cut(s) 21, 33, 233
BlsI GCNGC 3 cut(s) 22, 34, 234
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 2 cut(s) 41, 330
BmgT120I GGNCC 3 cut(s) 41, 137, 330
BmrFI CCNGG 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 200
BsaAI YACGTR 1 cut(s) 307
BsaJI CCNNGG 2 cut(s) 112, 139
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 112, 139
BseLI CCNNNNNNNGG 1 cut(s) 146
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 151
BseXI GCAGC 3 cut(s) 7, 19, 244
BsgI GTGCAG 1 cut(s) 251
BshFI GGCC 2 cut(s) 138, 194
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 111
BsnI GGCC 2 cut(s) 138, 194
Bsp143I GATC 1 cut(s) 152
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 1 cut(s) 12
BspANI GGCC 2 cut(s) 138, 194
BspCNI CTCAG 1 cut(s) 352
BspPI GGATC 1 cut(s) 160
BsrBI CCGCTC 1 cut(s) 14
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 112, 139
BssMI GATC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 156
BstBAI YACGTR 1 cut(s) 307
BstDEI CTNAG 2 cut(s) 121, 339
BstDSI CCRYGG 1 cut(s) 112
BstKTI GATC 1 cut(s) 155
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 1 cut(s) 152
BstMWI GCNNNNNNNGC 2 cut(s) 20, 29
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 7, 19, 244
BstX2I RGATCY 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 113
BstYI RGATCY 1 cut(s) 152
BsuRI GGCC 2 cut(s) 138, 194
BtgI CCRYGG 1 cut(s) 112
Cfr13I GGNCC 3 cut(s) 41, 137, 330
Csp6I GTAC 3 cut(s) 102, 144, 204
CviAII CATG 2 cut(s) 113, 319
CviJI RGCY 6 cut(s) 20, 138, 167, 194, 220, 343
CviKI_1 RGCY 6 cut(s) 20, 138, 167, 194, 220, 343
CviQI GTAC 3 cut(s) 102, 144, 204
DdeI CTNAG 2 cut(s) 121, 339
DpnI GATC 1 cut(s) 154
DpnII GATC 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco130I CCWWGG 1 cut(s) 112
Eco47I GGWCC 2 cut(s) 41, 330
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 116, 322
FaiI YATR 9 cut(s) 114, 180, 189, 252, 267, 314, 320, 324, 326
FatI CATG 2 cut(s) 112, 318
Fnu4HI GCNGC 3 cut(s) 21, 33, 233
Fsp4HI GCNGC 3 cut(s) 21, 33, 233
GluI GCNGC 3 cut(s) 21, 33, 233
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 2 cut(s) 138, 194
Hin1II CATG 2 cut(s) 116, 322
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 77, 211, 290
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 158
Hpy8I GTNNAC 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 54, 343
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 4 cut(s) 4, 35, 232, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 29
HpyF3I CTNAG 2 cut(s) 121, 339
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 116, 322
Kzo9I GATC 1 cut(s) 152
LmnI GCTCC 1 cut(s) 19
LpnPI CCDG 7 cut(s) 30, 117, 126, 132, 153, 153, 269
Lsp1109I GCAGC 3 cut(s) 7, 19, 244
MaeII ACGT 1 cut(s) 306
MalI GATC 1 cut(s) 154
MbiI CCGCTC 1 cut(s) 14
MboI GATC 1 cut(s) 152
MboII GAAGA 4 cut(s) 39, 75, 78, 173
MflI RGATCY 1 cut(s) 152
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 3 cut(s) 143, 157, 205
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 20, 29
NcoI CCATGG 1 cut(s) 112
NdeII GATC 1 cut(s) 152
NlaIII CATG 2 cut(s) 116, 322
NspI RCATGY 1 cut(s) 322
PfeI GAWTC 2 cut(s) 77, 211
PkrI GCNGC 3 cut(s) 22, 34, 234
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Ppu21I YACGTR 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 3 cut(s) 41, 137, 330
PsuI RGATCY 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 103, 145, 205
RsaNI GTAC 3 cut(s) 102, 144, 204
RseI CAYNNNNRTG 1 cut(s) 111
SatI GCNGC 3 cut(s) 21, 33, 233
Sau3AI GATC 1 cut(s) 152
Sau96I GGNCC 3 cut(s) 41, 137, 330
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 141
SetI ASST 6 cut(s) 22, 222, 258, 309, 335, 345
SinI GGWCC 2 cut(s) 41, 330
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SsiI CCGC 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 112
TaiI ACGT 1 cut(s) 309
TfiI GAWTC 2 cut(s) 77, 211
TseI GCWGC 3 cut(s) 20, 32, 232
TspDTI ATGAA 2 cut(s) 69, 286
TspGWI ACGGA 1 cut(s) 343
VpaK11BI GGWCC 2 cut(s) 41, 330
XceI RCATGY 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.